Wyniki wyszukiwania

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Factors affecting in vitro formation of cormlets in Gladiolus hybridus Hort. and their field performance

100%

Kumar A., Palni L. M. S., Sood A.

Acta Physiologiae Plantarum

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2011

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tom 33

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nr 2

The effect of number of important factors on in vitro cormlet formation has been investigated in Gladiolus hybridus Hort. Sucrose concentration of 232 mM was found to be best for producing higher number of cormlets per flask, whereas, the average mass of a cormlet increased with increase in sucrose concentration. Amongst three cultivars (cvs), maximum number of cormlets produced per flask was recorded in cv ‘Her Majesty’, but the average cormlet mass was higher in case of cv ‘Bright Eye’. Although the number of cormlets produced was found to be marginally higher at 30°C when compared with 20°C, the average cormlet mass was higher at the lower temperature. Both the number of cormlets formed per culture flask as well as the average fresh mass of a cormlet increased with increase in the size (volume) of the culture flask used. The known inhibitors of gibberellin biosynthesis used in this study suppressed cormlet formation, and the maximum inhibition was recorded in case of maleic acid hydrazide. Polyamines were found to be beneficial for cormlet formation, and amongst the polyamines used, incorporation of spermidine in the culture medium resulted in maximum number of cormlet formation per culture flask. Field trials indicated that the performance of such in vitro produced cormlets was comparable to that of conventionally produced cormels of the same weight range. The plants raised from in vitro produced cormlets were found to be morphologically similar to the mother plant.

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Podophyllotoxin content in Podophyllum hexandrum Royle plants of known age of seed origin and grown at a lower altilude

88%

Pandey H., Nandi S. K., Kumar A., Palni U. T., Palni L. M. S.

Acta Physiologiae Plantarum

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2007

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tom 29

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nr 2

Podophyllum hexandrum Royle, an important alpine herb, and a source of the highly valued aryltetralin-type lignan, podophyllotoxin, has been subjected to heavy collection from the wild due to ever increasing demand. The present study deals with an attempt to bring this plant under cultivation at a relatively lower altitude and to evaluate (1) various growth parameters including above and below ground biomass accumulation, net assimilation rate and relative growth rate, etc., and (2) podophyllotoxin content from the resulting above and below ground biomass in seed raised plants of a known age series of 1–5 years. The podophyllotoxin content was estimated on the basis of HPLC analyses. The levels were found to increase with the plant age and the maximum amount was found in 5-year old plants. This study demonstrates that (1) seeds can be conveniently used for raising healthy propagules in easily approachable locations at a relatively lower altitude, and that the plants can be maintained in such sites over long periods, and (2) this approach of ‘‘conservation through cultivation’’ can be suggested as an effective tool for the management of this ‘‘critically endangered status’’ species.

3

GA3 induced flowering in Podophyllum hexandrum Royle: A rare Alpine medicinal herb

88%

Pandey H., Nandi S. K., Chandra B., Nadeem M., Palni L. M. S.

Acta Physiologiae Plantarum

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2001

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tom 23

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nr 4

Ograniczanie wyników

3 Acta Physiologiae Plantarum

3 Palni L. M. S.

2 Kumar A.

2 Nandi S. K.

2 Pandey H.

1 Chandra B.

1 Nadeem M.

1 Palni U. T.

1 Sood A.

1 2011

1 2007

1 2001

Factors affecting in vitro formation of cormlets in Gladiolus hybridus Hort. and their field performance

Podophyllotoxin content in Podophyllum hexandrum Royle plants of known age of seed origin and grown at a lower altilude

GA3 induced flowering in Podophyllum hexandrum Royle: A rare Alpine medicinal herb