Thirty nine canine adnexal tumours were histologically and immunohistochemically analysed. They were classified as trichoblastomas, trichoepitheliomas, sebaceous adenomas and epitheliomas, hepatoid gland adenomas and epitheliomas, and aporcine adenocarcinomas. Moreover, hepatoid gland angioadenoma and angioepithelioma were recognised. Studies of follicular tumours revealed coexpression of cytokeratin MNF116 and LP34, but sebaceous and hepatoid gland tumours as well as apocrine adenocarcinomas showed differences in the expression of both cytokeratins. All the tumours were negative for vimentin except two hepatoid gland adenomas in which coexpression of vimentin and cytokeratin was observed. Positive reaction for neuron-specific enolase (NSE) was observed in follicular tumours, whereas expression of α-smooth muscle actin (α-SMA) was found only in apocrine adenocarcinomas. Moreover, the presence of NSE observed in hepatoid gland adenomas was considered as non-specific just as both glial fibrillary acidic protein in sebaceous gland tumours and hepatoid gland adenomas, and also S100 protein in sebaceous adenomas. All the examined tumours showed lack of expression of both desmin and factor FVIII. Thus, among the used antibodies, cytokeratins, vimentin, NSE, and α-SMA play a main role in the evaluation of canine adnexal histogenesis.
The aim of the study was an attempt to elucidate canine haemangiopericytoma histogenesis based on immunohistochemical analysis using a range of commercially available antibodies. Seventeen canine haemangiopericytomas were examined. Histological analysis revealed the presence of spindle cells arranged in a circular pattern around capillaries (.fingerprint. pattern) or interlacing bundles. Fingerprint patterns were the distinguishing feature in all examined haemangiopericytomas. Immunohistochemical analysis revealed positive expressions of vimentin, neuron-specific enolase (NSE), glial fibrillary acidic protein (GFAP), S-100 protein (S-100) and smooth muscle actin (a-SMA). Negative reactions in tumor cells were observed for cytokeratin (CK), desmin (DES), epithelial membrane antigen (EMA) and VIII-related antigen factor (FVIII). Positive expressions of neuronal markers may not necessarily reflect the neural origin of tumors due to their low specificity. Immunoreactivity for α-SMA and DES negativity may suggest the presence of vascular smooth muscle cells or cells of pericytic origin. Moreover, the α-SMA antibody may be useful for a differential diagnosis of canine haemangiopericytomas.
Цель труда состояла в определении развития инфекции у свиней после экспериментального заражения их перорально высокой дозой Myc. аvium серогип 2 лиоо Мус. intracellulare серотип 8. Аллергические макроскопические и микроскопические исследования провели на 16 каоанчиках возрастом 3 мес., разделенных на 2 равные группы. Животных подвергли убою через 42 и 84 дня от момента заражения тромбовали по 2 свиней из каждой группы. После убоя у всех зараженных свиней подтвердили в лимфатических узлах грыжейки типичные микроскопические и макроскопические изменения, соответственно через 42 и 84 дня эксперимента. Состояние туберкулиновой аллергии отметили на 84 день после заражения только у свиней зараженных Мус. avium серотип 2.
The goal of the studies was to evaluate the protective value of vaccines against pasteurellosis in rabbits. The vaccines comprised antigens to serotypes 3 and 12 of Pasteurella multocida with two different adjuvants: emulsigen and aluminium hydroxide gel. The two vaccines given subcutaneusly either once or twice at a dose of 1 ml protected rabbits markedly against intranasal infection with the virulent strains of examined serotypes. A substantial efficacy of these vaccines was supported by the occurrence of specific precipitins against somatic antigens of serotypes 3 and 12 present in the sera of immunised rabbits even 5 weeks after vaccination
Neoplastic changes characteristic of Marek's disease (MD) in the geese flock were described. The investigations were performed on White Italian reproductive geese kept on a farm where MD was previously diagnosed in broilers. Neither antibodies against MD virus (MDV) were detected by AGID method, nor MDV antigen was found by RID. The histopathological examination revealed the presence of lymphoid infiltrations characteristic of MD in all examined tissues. No lesions typical for avian leukosis or reticuloendotheliosis were observed. PCR products characteristic of meq, and ICP4 and pp38 genes were not observed, but real-time PCR for gB gene of MDV were positive in DNA samples from visceral organs. The realtime PCR results may indicate the presence of a new MDV variant or a new herpesviral infection among geese.
Для исследований использовали 6 сероотрицательных подопытных хряков и 180 проб эякулятов воспроизводительных хряков с известным уровнем противотел НА1 анти-РРУ в сыворотке. Подопытных животных инфицировали штаммом ПАПЬ-8 парвовируса свиней с титром ТСШ50= 10-, 00/0,2 мл. 2 хряков инфицировали перорально и внутрь носа, 2 — внутрь препуция и яичек. Каждый раз вводили 8 мл суспензии вируса с вышеприведенным титром. Для исследований брали кровь, семя, ткань яичек и придатков семенников. Упомянутый биологический материал получали через 3, 7 и 14 дней после инфекции. Наличие вирусного антигена отметили лишь в ткани яичка 2 хряков, инфекциро- ванных внутрь яичка, и 1 хряка, инфецированного внутрь препуция. Ни у одного из этих животных не обнаружили наличия РРУ в ткани придатков семенников. Не изолировали парвовируса из какого-либо эякулята, подвергнутого исследованиям. Результаты исследований показывают, что семя хряков является мало вероятным вектором в цепи пар- вовирусных инфекций свиней.