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Rajtar, G.: Influence of chronic ethanol treatment and the abstinence period, on the ethanol elimination by the .isolated perfused rat liver. Acta physiol, pol., 1988; 39 (4): 276-280. Rats received ethanol as their only (Irinking, fluid lor 4 weeks in a concentration ranging from 6-20%. After the ethanol-free intervals of 24, 48, 72, 96, 120 and 144 hours, the livers wereusolated and perfused with 100 ml of perfusion fluid with an addition of ethanol (0.2% f.c.) and the elimination of ethanol from the perfusate was studied. The livers of animals chronically exposed to ethanol showed significant diminution of ethanol elimination from perfusate after 72, 96 and 120 hours of withdrawal. After 144 hours the rate of the ethanol elimination returned to the control level.
Rajtar G. and Kleinrok Z.: Influence of some psychotropic drugs on the ethanol elimination by the isolated liver of rats chronically fed with ethanol. Acta Physiol. Pol. During a 4-week period the rats received ethanol (EtOH), as their only drinking fluid, in a concentration ranging from 6% to 20%. In the period of 72 hours after EtOH withdrawal the rats received diazepam (DZP), imipramine (IMI) or caffeine (CAFF) i.p. twice a day in a 12-hours interval. In the experiments carried out on the livers isolated from these rats, we observed the diminution of the rate of EtOH elimination from the perfusate by the livers of DZP and IMI treated rats. CAFF did not change the rate of EtOH elimination.
Rajtar G. and Kleinrok Z.: Effect of chronic ethanol treatment and the abstinence period on the ethanol elimination by isolated rat liver lesioned by carbon terachloride Acta Physiol. Pol. The study was carried out on perfused livers isolated from rats receiving ethanol (EtOH) as their only drinking fluid for the period of 4 weeks. Twelve, 24, 72 and 120 hours after EtOH withdrawal the livers were isolated and perfused with 100 ml of perfusion mixture with addition of EtOH (0.2% final concentration). After 12 hours of EtOH withdrawal acceleration of the EtOH elimination from perfusate was observed. It returned to the control level after 24 hours, but after 72 and 120 hours of abstinence the rate of EtOH elimination from perfusate was found to diminish. CC1₄ injected to the rats in doses of 2 and 5 mmoles/kg once a week for the period of 4 or 8 weeks, resulted in decreased EtOH elimination from the perfusate. In the EtOH-drinking group previously treated with CC1₄ we found that irrespective of the time of EtOH withdrawal, EtOH elimination did not differ from that in the respective CC1₄ treated group, only 12 hours after its withdrawal EtOH elimination was decreased in livers injured with CC1₄ in dose of 5 mmoles/kg.
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