Wyniki wyszukiwania

1

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Bioróżnorodność grzybów igliwia sosny zwyczajnej porażonej przez jesienną osutkę sosny

100%

Behnke-Borowczyk J.

Sylwan

|

2018

|

tom 162

|

nr 04

Needle casts belong to the fungal diseases of the assimilation apparatus, which affect the success and quality of the renewal of pine stands. The aim of this study was to investigate the diversity of communities of fungi, which occurred on Scots pine needles. Samples were collected in autumn 2015 from Scots pine saplings from Przedborów Forest District (17°58‵34‵‵E 51°31‵57‵‵N). Material was sequenced for DNA using Ilumina system. The results were compared with the NCBI base sequences. The results show significant dominance of fungi from Ascomycota division. The taxa responsible for the occurrence of Scots pine needle cast disease were also found. In this group, a significant share characterized the following species: Lophodermium pinastri, Cyclaneusma minus, Lophodermium seditiosum, Sydowia polyspora, Cenangium ferruginosum and Beauveria bassiana. The presented research should enhance the knowledge about the diversity of fungi inhabiting pine needles and about the contribution of pathogenic taxa, which can be used in a more efficient use of protective measures in forest management practice.

2

Wpływ metodyki badań na ocenę struktury zbiorowisk mikroorganizmów w glebie leśnej

63%

Behnke-Borowczyk J., Kwasna H.

Sylwan

|

2016

|

tom 160

|

nr 06

Two different communities of microorganisms were identified in soils by application of the classical method of fungi isolation (soil dilution, culturing on artificial media, morphotyping) and a molecular method (extraction of the environmental DNA, amplification with universal primers NS1 and NS2, cloning and sequencing of representative clones). No organisms were common to both communities. Apart from rare representatives of the Animalia, communities included single fungus−like Eucarya belonging to the Protista, Class Oomycota, and numerous fungi belonging to Chytridiomycota, Zygomycota, Ascomycota and Basidiomycota orders. In total, 88 species were identified in four soil samples. Fungi were mostly Ascomycota. The classical method was particularly effective in detection of fungi important for creation of phytosanitary conditions of soil, i.e. antagonists (Penicillium, Tolypocladium and Trichoderma) and potential stimulants (dark−pigmented Hormiactis candida, Humicola spp. and Phialophora spp.) of phytopathogens (including the common forest genera Armillaria and Heterobasidion). Application of the classical method allowed the detection of mycorrhizal Ascomycota from the genus Oidiodendron. Application of the molecular method allowed the detection of 13 mycorrhizal Basidiomycota. Although primers NS1 and NS2 were designed from a match with DNA of culturable organisms, they also amplified the DNA of non−culturable organisms. This emphasizes their potential usefulness in studies of the biodiversity of microorganisms in environmental samples. The shortage of reference sequences in the database discourages use of the 18S rDNA region in studies on fungal communities. The studies on the biodiversity of microorganisms need the application of a few independent methods of detection and identification.

3

Grzyby glebowe i ich znaczenie

63%

Behnke-Borowczyk J., Kwasna H.

Sylwan

|

2010

|

tom 154

|

nr 12

846-850

4

Solaryzacja jako metoda zwalczania patogenów glebowych w szkółkach leśnych

63%

Belka M., Behnke-Borowczyk J.

Acta Scientiarum Polonorum. Silvarum Colendarum Ratio et Industria Lignaria

|

2020

|

tom 19

|

nr 3

5

The identification of fungal species in dead wood of oak

63%

Behnke-Borowczyk J., Wolowska D.

Acta Scientiarum Polonorum. Silvarum Colendarum Ratio et Industria Lignaria

|

2018

|

tom 17

|

nr 1

6

Zastosowanie regionu ITS1/2 rDNA i 18S rDNA do badania mykobioty gleby leśnej

63%

Behnke-Borowczyk J., Kwasna H.

Sylwan

|

2016

|

tom 160

|

nr 07

The aim of the studies was to check the usefulness of ITS1/2 rDNA and 18S rDNA regions in the molecular investigation of forest soil microbiota structure. Soil studied, originated from a 1−year−old plantation and a 40−year old stand of Scots pine located in Bierzwnik and Międzychów forest districts located 200 km apart. The hypothesis assumed that both approaches lead to the discovery of abundant microbiota communities with different structures and with rare common species. The environmental DNA was extracted with a Power Soil ® DNA Isolation Kit from two soil samples in each site. The ITS1/2 rDNA was amplified with specific primers ITS1 and ewfitsrev 1, and 18S rDNA with universal primers NS1 and NS2. PCR products were cloned into pGEM−T Easy. Inserts were primarily selected in blue/white screening on a X−gal medium. Representative clones were further selected in two separate RFLP analyses with HhaI and BsuRI restriction enzymes. Representative clones purified and sequenced using the Sanger Method in the DNA Research Centre (Poznań). Each sequence was identified to the lowest taxonomic rank. Ninety to 233 clones with DNA of 5−44 taxa including 3−37 taxa of fungi were obtained from 4 samples of soil. After application of ITS1/2 rDNA and 18S rDNA, the fungal DNA was detected respectively in 89,60−100,00% and 11,77−64,8% clones and the number of fungal species detected was respectively 12−37 and 3−19. Fungi were represented by four orders: Chytridiomycota, Zygomycota, Ascomycota and Basidiomycota. Both primers also amplified also DNA of other organisms (mostly from Animalia and Protista Kingdom) represented by 0−9 taxa. If compared, the application of forest soil microbiota structure with ITS1/2 rDNA and 18S rDNA led to detect a lower abundance of fungi and a bigger abundance of other organisms. Considering the higher number of clones and taxa recognized, the region of ITS1/2 rDNA was more effective in the studies of the soil microbiota structure. The region of 18S rDNA was efficient in local detection of Chytridiomycota and Zygomycota and of rare species of fungi from Ascomycota and Basidiomycota. Despite the deficiency of NCBI database the use of the 18S rDNA region in studies on fungal community the region should be included in molecular studies of fungal diversity. It is concluded that studies on the biodiversity of soil microorganisms need the application of a few independent methods of detection and identification.

7

Populacja mączniaka prawdziwego dębu w zachodniej Polsce

63%

Behnke-Borowczyk J., Baranowska-Wasilewska M.

Acta Scientiarum Polonorum. Silvarum Colendarum Ratio et Industria Lignaria

|

2017

|

tom 16

|

nr 4

8

Goidanichiella sphaerospora, the world's second record

63%

Kwasna H., Behnke-Borowczyk J.

Acta Mycologica

|

2010

|

tom 45

|

nr 1

Aspecimen of Goidanichiella sphaerospora was found in Pinus sylvestris forest soil in Międzychód, northwestern Poland (52.601, 15.889883), in October 2009. This is the world’s second record of G. sphaerospora. Bimorphic conidial heads and conidia are reported for the first time. Goidanichiella sphaerospora forms Aspergillus- and Penicillium-like conidial heads. Conidia formed at +24oC are oval to ellipsoidal, often apiculate, smooth, rather thick-walled, hyaline, with one oil drop inside, 3-4 × 2-3 μm. Additional ellipsoidal to cylindrical, thin-walled, 4-6.5 (-8) × 2-3.0 μm conidia are formed only after incubation for at least 7 days at +4oC in darkness.

9

Armillaria population in flood-plain forest of natural pedunculate oak showing oak decline

51%

Szewczyk W., Kwasna H., Behnke-Borowczyk J.

Polish Journal of Environmental Studies

|

2016

|

tom 25

|

nr 3

We studied the Armillaria population in a forest of natural pedunculate oak subject to periodic flooding and showing symptoms of oak decline. The study area was in Wołów Forest District in the Oder River Valley flood plain. Armillaria DNA was isolated by direct extraction from rhizomorphs and mycelium in soil and root samples. Armillaria species were identifi ed by PCR-RFLP of IGS-1 rDNA using the restriction enzymes AluI, TaqI, MspI, and BsmI. Rhizomorphs were found in 10 out of 12 sites. The number of soil rhizomorphs collected (0-28 in 100 kg of soil per sampling site) often differed significantly between sites. The mean number of soil rhizomorphs per sample was signifi cantly less in flooded sites (0.30 or 0.46) than in the non-flooded control (1.16), and insignifi cantly less in sites flooded once (0.30) than in sites flooded twice (0.46). Comparison of the RFLP patterns for Armillaria samples from the oak forest with those for Armillaria testers showed them to be A. borealis (two DNA samples), A. cepistipes (seven samples), A. gallica (150 samples), and A. solidipes (one sample). RFLP patterns for Taq1 were unfamiliar, since this enzyme has rarely been used, while A. cepistipes gave a rare pattern with AluI, known previously only from Japanese isolates of this fungus. Since AluI and TaqI each produced a similar pattern for A. borealis and A. solidipes, an additional functionally efficient enzyme, BsmI, was used. These results and those from other Armillaria population studies showed that flooding affected the abundance of soil rhizomorphs, but not the diversity of Armillaria species. The decreased abundance of Armillaria rhizomorphs in soil subjected to flooding may be indicative of the flooded soil syndrome in which general loss of fungi occurs. Flooding may still be considered, however, to be a short-term inciter and stress inducer, occurring between “primary” factors and “secondary” agents that predispose the tree to infection. This is the first report on populations of Armillaria in declining oaks in flood-plain forests in the northern hemisphere.

10

Effect of fungi on the destruction of historical parchment and paper documents

51%

Kwasna H., Karbowska-Berent J., Behnke-Borowczyk J.

Polish Journal of Environmental Studies

|

2020

|

tom 29

|

nr 4

11

Mączniak prawdziwy dębu

51%

Baranowska-Wasilewska M., Behnke-Borowczyk J., Szewczyk W., Wajchman S.

Przegląd Leśniczy

|

2015

|

tom 25

|

nr 03

12

Gatunki Phytophthora i Pythium w glebie i w korzeniach dębu szypułkowego na terenach popowodziowych w Nadleśnictwie Wołów

51%

Kwasna H., Szewczyk W., Behnke-Borowczyk J.

Sylwan

|

2015

|

tom 159

|

nr 07

Phytophthora and Pythium species (Oomycota) are known to be serious pathogens of forest trees. Little is known, however, about their presence in Polish oak forests and their role in the oak decline, especially in the flooded areas. The aim of this study was (1) to detect and compare populations of microorganisms from Oomycota and fungi in roots and soil of healthy and declining pedunculate 30−126 years old oak stands, which had been flooded by the Odra River for three months in 1997 and one month in 2010, (2) to demonstrate the relationship between different chemical factors of soil and the occurrence of microorganisms, and (3) to assess the contribution of Oomycota to oak decline. Study was carried out in Wołów Forest District (south−western Poland). Microorganisms were isolated from fine (1−5 mm in diameter) roots and non−rhizosphere soil collected from 0−50 cm horizon. Isolation procedure from roots included surface sterilization and plating the root segments on the nutrient agar. An oak leaf baiting method was used for isolation of Oomycota from soil. Identification of microorganisms was based on morphology and sequencing of the ITS1/2 rDNA. Oomycota was represented by: Globisporangium, Phytophthora and Pythium and fungi mostly by: Aspergillus, Chaetosphaeria, Cylindrocarpon, Mortierella, Mucor, Penicillium, Trichoderma and Umbelopsis. Globisporangium attrantheridium (syn. Pythium attrantheridium) occurred locally and was found in roots of only one flooded oak. This is the first record of this species on oak and in Poland. G. intermedium, P. gibbosa/P. gregata, P. plurivora and Pythium sp. were found in soil of flooded and non−flooded (control) oak stands. The organisms occurred in podzolized brown soils, brown alluvial soils and gley soils that had most nitrogen (21−60 mg/kg), various amounts of calcium (61.1−347.6 mg/100g) and moderate acidity (pH=3.85−4.2). There was often a significant association between exposure to flood and the health status of oak trees assessed by the scale of defoliation. Oomycota seemed to be only moderately associated with increased tree defoliation as a symptom of oak decline.

13

Metody molekularne stosowane w badaniu różnorodności mikroorganizmów glebowych

51%

Behnke-Borowczyk J., Kwasna H., Belka M.

Sylwan

|

2012

|

tom 156

|

nr 04

14

Amerykański obywatel - mączniak prawdziwy dębu

51%

Baranowska-Wasilewska M., Behnke-Borowczyk J., Wajchman S., Wynarowicz M.

Ekonatura

|

2015

|

nr 08

15

Zastosowanie technik biologii molekularnej do detekcji Erysiphe alphitoides (Griffon & Maubl.) U. Braun and S. Takam. w organach roślinnych

51%

Roszak R., Baranowska M., Belka M., Behnke-Borowczyk J.

Sylwan

|

2019

|

tom 163

|

nr 09

Erysiphe alphitoides causes the most common disease of assimilation apparatus of oaks of different age. It is believed that the pathogen overwinters in buds of the host plant or in the cracks of the bark. The aim of the study was to search for the presence of E. alphitoides in buds, leaves, and wood of sessile oak shoots using molecular techniques. Two hypotheses have been tested: (i) oaks are infected by E. alphitoides, and (ii) the pathogen overwinters in the host plant buds. The samples used in the study were collected form sessile oak trees (Miradz Forest District; 52°41'23.197"N, 18°25'33.942"E) in 2017, and consisted of dormant buds (collected in May), young leaves (collected in June), leaves with visible symptoms of the disease (collected in August), shoot with sip and surface layers of wood (collected in December). Additional part of buds was collected in May 2018. The PCR reaction was carried out with primers specific for E. alphitoides and E. hypophylla. For species identification Sanger method was used. The resulting sequences were compared using BLAST algorithm with reference sequences deposited in the NCBI database. Sequences from isolates obtained from leaves showed 97−99% similarity to the reference sequence of E. alphitoides. Pathogen did not occur in the superficial layers of shoots and buds. As some studies show, at very low temperatures (below –20°C), the mycelium of the pathogen dies, therefore further study should be undertaken on wintering of the pathogen causing the powdery mildew disease on oaks. It also should be examined whether the pathogen overwinters in the form of bagnial spores produced in chasmothecia overwintering on leaves, in the leaf buds, or in bark cracks, and whether the wintering site of the pathogen is related to the age of trees and or to the stands where the trees grow.

16

Kilka słów o hubie sosny

51%

Baranowska-Wasilewska M., Behnke-Borowczyk J., Szewczyk W., Gorawski R.

Ekonatura

|

2013

|

nr 11

17

Mykobiota krzewów jałowca Juniperus x media z objawami zamierania na terenie oczyszczalni ścieków w Poznaniu

51%

Behnke-Borowczyk J., Kwasna H., Szewczyk W., Zatorski J.

Leśne Prace Badawcze

|

2019

|

tom 80

|

nr 4

18

Odnowienie sosny zwyczajnej na wybranych powierzchniach w Nadleśnictwie Niedźwiady

51%

Skiba R., Korzeniewicz R., Behnke-Borowczyk J., Baranowska M.

Przegląd Leśniczy

|

2020

|

tom 30

|

nr 12

19

Kłopotliwe sąsiedztwo gruszy i jałowca

51%

Baranowska-Wasilewska M., Behnke-Borowczyk J., Wynarowicz M.

Ekonatura

|

2014

|

nr 08

20

Populations of Armillaria species in pine plantations in west-central Poland

51%

Szewczyk W., Kwasna H., Behnke-Borowczyk J.

Dendrobiology

|

2015

|

tom 74

Two diploid isolates of Armillaria gallica and 143 diploid isolates of A. solidipes were obtained from 145 samples of rhizomorphs, fruit bodies and infected wood of sessile oak and Scots pines from 5–10-year-old Scots pine plantations in three Forest Districts located 100–350 km apart in west-central Poland. Based on pairings among the 145 isolates, 18 somatic compatibility groups (genets) of A. solidipes were distinguished in the three plantations. Sequencing of ITS1/2 rDNA of 18 isolates representing the 18 genets delineated four nuclear haplotypes. This suggests that many of the A. solidipes genets are closely related and were possibly established by sib-related basidiospores. With a few exceptions there was general geographical specialization of genets but not haplotypes. Geographical specialization of genets and the dominance of single haplotypes suggest that the A. solidipes population results from clonal rather than sexual reproduction. Sequencing of the ITS1/2 and IGS-1 rDNA showed small nucleotide diversity in ITS1/2 rDNA and much more diversity in IGS-1 rDNA of the isolates of A. solidipes studied. However, none of these regions has sufficient resolution for the clear differentiation of A. solidipes from A. borealis. The sequences of the EF 1-alpha gene showed high interspecific variability in Armillaria species and very low intraspecific variability in A. solidipes. This gene is the most appropriate for reliable identification of biological species and subgroups of Armillaria. Its application is the most useful in ecological and epidemiological studies of Armillaria.

Ograniczanie wyników

Bioróżnorodność grzybów igliwia sosny zwyczajnej porażonej przez jesienną osutkę sosny

Wpływ metodyki badań na ocenę struktury zbiorowisk mikroorganizmów w glebie leśnej

Grzyby glebowe i ich znaczenie

Solaryzacja jako metoda zwalczania patogenów glebowych w szkółkach leśnych

The identification of fungal species in dead wood of oak

Zastosowanie regionu ITS1/2 rDNA i 18S rDNA do badania mykobioty gleby leśnej

Populacja mączniaka prawdziwego dębu w zachodniej Polsce

Goidanichiella sphaerospora, the world's second record

Armillaria population in flood-plain forest of natural pedunculate oak showing oak decline

Effect of fungi on the destruction of historical parchment and paper documents

Mączniak prawdziwy dębu

Gatunki Phytophthora i Pythium w glebie i w korzeniach dębu szypułkowego na terenach popowodziowych w Nadleśnictwie Wołów

Metody molekularne stosowane w badaniu różnorodności mikroorganizmów glebowych

Amerykański obywatel - mączniak prawdziwy dębu

Zastosowanie technik biologii molekularnej do detekcji Erysiphe alphitoides (Griffon & Maubl.) U. Braun and S. Takam. w organach roślinnych

Kilka słów o hubie sosny

Mykobiota krzewów jałowca Juniperus x media z objawami zamierania na terenie oczyszczalni ścieków w Poznaniu

Odnowienie sosny zwyczajnej na wybranych powierzchniach w Nadleśnictwie Niedźwiady

Kłopotliwe sąsiedztwo gruszy i jałowca

Populations of Armillaria species in pine plantations in west-central Poland