Ograniczanie wyników

Czasopisma help

  1. 43 Acta Neurobiologiae Experimentalis

  2. 2 Acta Biochimica Polonica

  3. 2 Biotechnologia

  4. 2 Journal of Physiology and Pharmacology

  5. 1 Acta Physiologica Polonica

Więcej...
Autorzy help

  1. 52 Przewlocki R.

  2. 11 Bilecki W.

  3. 11 Przewlocka B.

  4. 10 Korostynski M.

  5. 9 Solecki W.

Więcej...
Lata help

  1. 8 2017

  2. 1 2015

  3. 1 2014

  4. 3 2013

  5. 3 2011

Więcej...
Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 52

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Uzależnienia opioidowe, mechanizmy, terapia

100%
Przewlocki R.
Wszechświat
|
2017
|
tom 118
|
nr 01-03
2

Functions of opioids in the brain

88%
Przewlocki R.
Acta Neurobiologiae Experimentalis
|
1997
|
tom 57
|
nr 5
3

Acute and chronic effects of morphine on creb regulation in Neuro2a MOR1A cells

63%
Bilecki W., Przewlocki R.
Acta Neurobiologiae Experimentalis
|
1999
|
tom 59
|
nr 3
4

Effect of LiCl on PENK mRNA and cAMP level in bovine chromaffin cells

63%
Dziedzicka M., Przewlocki R.
Acta Neurobiologiae Experimentalis
|
1992
|
tom 52
|
nr 3
5

The effect of opioids on the regulation of transcription at the cellular level

63%
Bilecki W., Przewlocki R.
Acta Neurobiologiae Experimentalis
|
2001
|
tom 61
|
nr 3
6

Effect of opioids on Ca2plus-cAMP responsive element binding protein

63%
Bilecki W., Przewlocki R.
Acta Neurobiologiae Experimentalis
|
2000
|
tom 60
|
nr 4
Ca2+ /CAMP response element binding protein (CREB) is an important factor linking the opioid-regulated secondary messenger systems to alterations in gene expression. Opioids regulate CREB level, its phosphorylation and binding to its corresponding response element in the promoters of several genes implicated in drug addiction. CREB mediates the action of opioids on the expression of several genes in brain regions responsible for drug-seeking behavior and manifestation of signs of dependence. Moreover, alterations in CREB level can affect the rewarding properties of morphine and regulate the self-administration of cocaine. At the cellular level CREB acts as convergence point for different cellular pathways. Opioids affect two different intracellular mediator systems: inhibitory - connected with cAMP, and stimulatory - involving calcium and the PKC pathway. Both can affect CREB but in different phases of opiate action. The presence of this biphasic mechanism can explain the phenomenon of the induction of some CRE-controlled genes after both acute and chronic morphine administration. Cellular studies also highlight the relevance of other ATF/CREB family members which can affect Ca /cAMP response element (CRE) controlled transcription as well as other transcription factors which make the opioid induction longer lasting.
7

Endogenne systemy opioidowe

63%
Przewlocki R.
Postępy Biochemii
|
1985
|
tom 31
|
nr 3-4
8

Regulation of extracellular signal-regulated kinases activity by morphine in mouse brain

51%
Wawrzczak-Bargiela A., Bilecki W., Przewlocki R.
Acta Neurobiologiae Experimentalis
|
2007
|
tom 67
|
nr 3
9

Changes in the opioid systemsand CRF in the amygdala in various models of drug dependence

51%
Turchan J., Maj M., Przewlocki R., Przewlocka B.
Acta Neurobiologiae Experimentalis
|
1999
|
tom 59
|
nr 3
10

Lucocorticoid receptor stimulation modulates morphine addiction-like symptoms

51%
Marut L., Skupio U., Przewlocki R.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
11

Opioid systems in the brain of inbred mouse strains

51%
Gierek A., Ziolkowska B., Solecki W., Przewlocki R.
Acta Neurobiologiae Experimentalis
|
2007
|
tom 67
|
nr 3
12

The genetic background is responsible for response to natural reward

51%
Kubik J., Solecki W., Przewlocki R.
Acta Neurobiologiae Experimentalis
|
2009
|
tom 69
|
nr 3
The increased inclination to natural rewards like food and sensation seeking have been associated with elevated drug intake in humans. We aimed to compare four inbred mouse strains with known different susceptibility to drug addiction in respect to response to natural rewards. In this study, we investigated four inbred strains C57Bl/6J, DBA/2J, SWR/J and 129P3/J in the selfadministration of food (SAF) and operant sensation seeking (OSS) models in three paradigms: (1) the fi xed ratio operant task (FR), (2) the progressive ratio operant task and (3) the concurrent choice operant task. No difference in response to reward in SAF FR paradigm was found among the studied strains. In contrast C57Bl/6J mice performed better then DBA/2J and 129P3/J strains in OSS paradigm while SWR mice did not react at all. In turn, the highest score of correct choice in SAF was observed in 129P3/J mice. The systemic administration of dopamine D1 receptor antagonist SCH 23390 or opioid receptor antagonists (naloxone or naltrexone) reduced the response to reward in the C57Bl/6J and DBA/2J mice. Our data suggest that genotype background is responsible for reward-seeking in SAF and OSS models with C57Bl/6J mice being the most responsive to the reward. We observed also that food as a reward has different motivational value than sensation seeking. Also our study indicates that opioid and dopaminergic systems may be involved in the response to natural rewards.
13

Loss of mGluR5 receptors on dopamine D1-expressing neurons abolishes novelty-seeking behaviours

51%
Sikora M., Rodriguez J., Przewlocki R.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
High novelty seeking behaviour has been suggested to be related to altered dopaminergic activity, however the underlying mechanisms are still poorly understood. Here we investigate how glutamatergic modulation of dopaminoceptive neurons affects novelty seeking in a mouse model with selective knock down of metabotropic glutamate receptor 5 (mGluR5) in neurons expressing D1 receptors. Mutant mice (mGluR5KD-D1) displayed normal habituation to a novel environment (time F3.84=11.33; p<0.0001, genotype F4.86=1.29; p=0.2661). When a novel object was placed in the middle of the open field apparatus mGluR5KD-D1 mice spent significantly less time interacting with it compared to wild-type controls (t=28.39; p=0.0095). Moreover, mGluR5KD-D1 mice showed no operant sensation seeking behaviour. While over subsequent training sessions wild-type animals gradually increased the number of operant responses associated with presentation of a light and noise, mutant mice showed no such behaviour (two-way ANOVA for active lever: genotype F1.90=22.06; p=0.001; session F10.90=4.5; p<0.0001; genotype × session F10.90=1.963; p=0.04). Decreased operant sensation seeking was not assosiated with higher anxiety as assessed by the elevated plus maze. Finally, mGluR5KD-D1 mice exhibited normal operant responding for food as a reinforcer (two-way ANOVA for active lever: genotype F1.45=0.93; p=0.359; session F5.45=19.09; p<0.0001; genotype × session F5.45=0.13; p=0.985). In conclusion, these results indicate mGluR5 receptors located on dopamine D1 receptor-expressing neurons are essential in novelty-seeking behaviour.
14

Alcohol drinking under intermittent access in group-housed C57BL/6J mice

51%
Smutek M., Rodriguez-Parkina J., Turbasa M., Przewlocki R.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr Suppl.1
Models of alcohol drinking in rodents are useful in determining factors underlying uncontrolled alcohol abuse. Here we describe a new model, which overcomes a limitation of previous approaches, the necessity of studying animals in isolation. Over the course of 4–5 weeks group-housed mice intermittently received free or instrumental access to 12% alcohol in the IntelliCage system. Animals developed stable alcohol preference which was similar across 6 cohorts tested (46.6 ± 12.8%). Compared to behaviors of singlehoused mice, we found no escalation of drinking over time, and no difference in alcohol preference between males and females, but comparable levels of alcohol preference to those previously reported. Motivation to obtain alcohol or saccharin measured under a progressive ratio schedule was initially similar, but the breakpoint decreased over consecutive sessions in case of alcohol (from 19.8 to 15.9). Conversely, addition of 0.03% quinine had a smaller impact on alcohol than saccharine intake (72.2% vs. 97.2% decrease). We observed that sequences of animal entries to alcohol-containing corners diverged from random distribution. Thus, this model applied to group-housed mice induces stable levels of alcohol drinking and allows to measure its motivational aspects as well as explore relations between social structure and drinking behavior.
15

Influence of the dorsal raphe nucleus stimulation on the electrical activity in the caudate nucleus and putamen in the rat brain

51%
Chlapowska M., Przewlocki R., Smialowski A.
Acta Physiologica Polonica
|
1979
|
tom 30
|
nr 1
16

Changes of ERK1 and ERK2 phosphorylation in hippocampus during morphine-induced conditioned place preference in mouse brain

51%
Wawrzczak-Bergiela A., Bilecka W., Przewlocki R.
Acta Neurobiologiae Experimentalis
|
2009
|
tom 69
|
nr 3
Extracellular signal-regulated kinases (ERK1 and ERK2) may play an important role in the molecular mechanisms of opiates addiction. Recently, the role of hippocampus in the process of addiction has attracted an attention as addictive drugs like morphine may affect the normal function of this region leading to a formation of aberrant learning. Our previous results demonstrated that acute and prolonged morphine treatment leads to changes of ERK1 and ERK2 phosphorylation. However, the specifi c pattern of alterations of ERK1 and ERK2 phosphorylation (which is required for ERKs activity) in the hippocampus, during the development of morphine reward has not been studied yet. We discovered that fi rst morphine conditioning increased ERK1 and ERK2 phosphorylation in CPP paradigm, but after third morphine session during CPP, we observed the decrease of ERK1 and ERK2 phosphorylation. Moreover, an increase of pERK2/pERK1 ratio correlated linearly with the expression of place preference following morphine administration. The changes of phosphorylation were observed in mossy fi bers, the structure, which is involved in spatial learning and memory. In hippocampus, the observed changes of ERK phosphorylation favoring mainly ERK2 activity together with the putative role of ERK2 in learning and long-term memory suggest that morphine-induced interactions within ERK pathway participate in reward-controlled learning. Research supported by Ministry of Science and Education Grant N401 066 31/168.
17

Gene expression profiling of psychotropic drugs effects in the brain

51%
Korostynski M., Piechota M., Przewlocki R.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr 1
Psychotropic drugs activate synchronized patterns of gene expression in the brain. These patterns are connected to biological processes involved in therapeutic as well as adverse drug effects. To reveal the transcriptional networks activated by different classes of psychotropic drugs we compared the effects of antidepressants (e.g. mianserine, fluoxetine), analgesics (e.g. morphine, heroin), psychostimulants (e.g. methamphetamine, cocaine) and antipsychotics (e.g. clozapine, haloperidol) on genomic profile in mouse (C57BL/6J) striatum. We applied a whole-genome microarray (Illumina WG-6) profiling to characterize time-course of transcriptome alterations following acute drug administration (1, 2, 4 and 8h after injection). We identified major drug-regulated expression patterns that are formed by inducible transcriptional networks, as for example: (1) CREB/SRF-dependent genes that appears to be related to dopaminergic activity the striatum, (2) the group of genes controlled at least in part via release of steroid hormones. The data were stored as raw values, fold of change versus saline and P value of drug versus saline comparison in the database (available at www. genes2mind.org). The database interface allows for multidimensional data analysis (PCA), search for drug using genomic signatures and visualize drug-regulated gene transcription patterns. Our results elucidates the networks of drug-induced genes that share common regulatory elements, functional relations and may provide novel diagnostic tools for prediction of drug effectiveness. This work was supported by EU grant LSHM-CT-2004-005166, POIG DeMeTer 3.1 and NN405 274137 grants.
18

Interactions between melanocortins and opioids. In vivo and in vitro studies

51%
Starowicz K., Przewlocka B., Przewlocki R.
Acta Neurobiologiae Experimentalis
|
2001
|
tom 61
|
nr 3
19

Long-term morphine self-administration schedule in intellicages as a preclinical model of opioid abuse in mice

51%
Skupio U., Sikora M., Turbasa M., Przewlocki R.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Chronic exposure to opiates induces various alterations in brain physiology that may lead to formation of dependence, tolerance and addiction. Commonly used approaches for modeling morphine dependence involve the use of conditioned place preference, which lacks voluntary intake of the drug, and morphine self-administration, which requires isolating the animals. Here, we describe a novel model of long-term morphine self-administration in C57BL/6J mice. METHODS:We have used IntelliCage (NewBehavior, Switzerland) system to observe the animals without unnecessary experimental intervention. The animals in two separate cages were allowed access to sweetened morphine (0.5 mg/ml) or saccharin solutions for 3 executive months with saccharin concentration being gradually lowered (from 0.2% to 0.02%). We behaviorally challenged animals to test for symptoms of compulsive morphine drinking, using paradigms like saccharin reduction, progressive ratio schedule and intermittent access to rewarding substance. RESULTS: We have observed stable preference to both saccharin and morphine throughout the drinking schedule. The animals performed significantly increased number of instrumental responses to obtain access to the bottle with morphine (progressive ratio schedule) and significantly more nose pokes in attempt to obtain rewarding substance during intermittent access procedure, when compared to saccharin group. What is more, morphine dependent animals exhibited a variety of spontaneous withdrawal symptoms that lasted up to 32 hours. CONCLUSIONS: This study demonstrate that our model reliably leads to stable morphine drinking while avoiding the limitations associated with testing isolated animals. Mice drinking morphine exhibit many of the symptoms of dependence and craving compared to control animals. Therefore, this model may be well suited to screening for the effects of genetic mutations or pharmacological treatments on morphine-induced behaviors.
20

Glucocorticoid receptor stimulation modulates morphine addiction-like symptoms

51%
Marut L., Skupio U., Przewlocki R.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: Morphine is widely used painkiller, however misuse of morphine may lead to the addiction. Stress system and glucocorticoids are thought to be involved in various aspects of addiction-like behavior. In animal models stressors and glucocorticoids facilitate acquisition of drug self-administration, increase their rewarding potential and promote relapse. AIM(S): The involvement of stress system in addiction is widely studied however the specific role of glucocorticoid receptor (GR) is still not fully understood. The aim of this study was to evaluate effects of GR stimulation on addiction-like symptoms induced by morphine. METHOD(S): We used dexamethasone (DEX), selective glucocorticoid receptor agonist, in self-administration (SA) and conditioned place preference (CPP) paradigms in mice. Mice were allowed to self-administer increasing doses of morphine. To test the influence of GR stimulation on morphine intake, we administered DEX (4 mg/kg). Also effects of DEX on morphine place preference was evaluated. 1 hour before morphine conditioning mice were pretreated with either DEX (4 mg/kg) or saline (SAL). RESULTS: DEX treatment resulted in significant increase in morphine intake. This effect seems to be specific for the drug, as at the same time, DEX treatment caused decrease in water intake. Interestingly, in CPP paradigm DEX pretreatment resulted in significant decrease of time spent in morphine-paired compartment of the apparatus. In control conditions SAL pretreatment did not affect morphine place preference. CONCLUSIONS: In CPP paradigm DEX attenuate morphine place preference that is a measure of drug rewarding properties. GR stimulation led to enhanced morphine SA. This result may indicate that after DEX mice need more drug to achieve the same reward. Taking together our result may suggest that GR stimulation decrease rewarding potential of morphine. FINANCIAL SUPPORT: Funding for this study was provided by Polish National Science Centre Grants: 2013/08/A/ NZ3/00848.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.