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Our study focused on the mitigation role of exogenously applied hydrogen peroxide (H₂O₂) in eliminating toxicty caused by salt (NaCl). Barley seeds were pretreated with 30 μM (micromolal) H₂O₂ for 24 hours and then exposed to increasing salt concentrations (0.0, 0.25, 0.275, 0.30 M). Morphogical and physiological changes in seed germination and seedling growth stages were compared between different treatments of salt in laboratory conditions. Adverse effects of salt during both germination and seedling growth stages were dependent on the concentration of the salt treatment. We found that the application of H₂O₂ effectively alleviated the salt-induced inhibition, and reduced the negative effects of salt on germination (germination index and vigor index), seedling growth stages (radicle and coleoptile lengths, fresh weight), and leaf parameters (stomata and epidermis counts, stomatal index, stomata sizes of adaxial and abaxial surfaces). The differences were statistically significant. Alleviating the effects of H₂O₂ increased in parallel with salt concentration (p≤0.05). However, under non-stress conditions (control), H₂O₂ didn’t have any effect on the investigated parameters (p≥0.05). Our results suggest that exogenous H₂O₂ application is involved in the resistance of barley to salt stress.
In this work, the effects of polyamine (cadaverine, putrescine, spermidine, spermine) pretreatments on the germination of barley seeds, seedling growth under saline (NaCI) conditions and on leaf anatomy of the seedlings grown for 20 days in pots with perlite containing different concentrations of NaCI prepared with Hoagland solutions following the germination period of 7 days were studied. The inhibitive effect of salt on seed germination and seedling growth was alleviated in varying degrees, and dramatically, by polyamine pretreatments at the levels of NaCI studied; particularly with lower levels of salt, the control seeds were able to germinate with great difficulty. Also, the successes of these pretreatments continued, even decreased, with higher levels of NaCI such that the control seeds showed no germination. On the other hand, on the various parameters of leaf anatomy of barley seedlings, interactions between pretreatments of polyamine and salinity, with some of these being statistically important, were observed.
The inhibitory effects on biological properties of maize (Zea mays L. saccharata Sturt) seeds treated with chlorantraniliprole (CAP) were examined under greenhouse conditions. Maize seeds were exposed to environmentally relevant doses (0.08, 0.1, 0.2, 0.3, 0.4, and 0.5 ppm) of CAP. On day 7 of germination, morphological, anatomical, and physiological responses were determined. The most devastating inhibitory effect on germination parameters of maize seeds was on coleoptile length (90%) in 0.5 ppm insecticide concentration. Increasing concentrations of CAP resulted in decreased stomata density, compared with control. Similar results were also observed in stomata dimensions. On the other hand, high concentrations of CAP led to phytotoxic effects on photosynthetic pigments such as chlorophyll a, chlorophyll b, and carotenoid. On the contrary, the amount of anthocyanin and proline increased in proportion to increasing dosages, despite a reduction in growth. The results showed that all investigated parameters (except for proline and anthocyanin) significantly decreased with increasing insecticide dosages, compared to control (p<0.05). The changes, particularly stomatal responses, and the amount of proline and anthocyanin on the leaves of the plants exposed to CAP stress, were determined as more sensitive parameters to detect insecticidal damage.
The current study was undertaken to establish reference values of intraocular pressure in clinically normal Turkish Shepherd Dogs with the rebound tonometer (TonoVet®) and the applanation tonometer (Tono-Pen Vet®). Twenty-eight dogs (14 male and 14 female, aged 9 months-9 years, 56 eyes) were used in this study. All animals were healthy and showed no abnormalities in ophthalmic examinations, which consisted of the Schirmer tear test, slit lamp bio-microscopy, and fluorescein staining. The animals were restrained manually without using systemic anesthetics or tranquilizers. IOP was measured in both eyes with a TonoVet® and a Tono-Pen Vet®. In normal dogs, the mean IOP values for all eyes were 17.63 ± 3.34 mmHg according to the TonoVet® and 14.95 ± 2.92 mmHg according to the Tono-Pen Vet®. There was no effect of sex, age, or eye side (right vs. left) on the results obtained with either the TonoVet® or the Tono-Pen Vet®. The mean IOP obtained with the rebound tonometer was 2-3 mmHg higher than that measured with the applanation tonometer. The reference data obtained in the present study may assist in diagnostic testing for ophthalmic diseases in Turkish Shepherd Dogs and may promote further studies in this area.
This r esearch was carried out along the banks of the Bogacayi River in the western part of Antalya in Turkey. We investigated the concentration, average distribution, and level of contamination of the sediments by heavy metals for monitoring purposes. In this study, 25 sediment samples from different stations were collected and analyzed along the Bogacayi’s banks. Heavy metals detected in order of abundance are Mg> Fe > Al > Ti> MN > Cr > Ni > Ba > V > Zr > Zn > Co > Cu > Pb > As > Mo > W > Cd > Sb > Ag. The average concentration of each heavy metal elements was compared to those of the earth crust, sandstone, ultrabasic rock, especially with the acceptable limit for Turkey, in order to determine their anomalies. The concentration of Cr in sample 24 was 19.85 times and Ni in sample 25 was 19.29-7.71 times higher than the acceptable limit for Turkey. In samples 24 (1.87 times) and 25 (1.85 times), Co was also higher than the acceptable limit for Turkey. As confi rmed by the coeffi cient correlation analysis, the PCA, anthropogenic activities is thought to have possibly contributed most of the Sb, Mo, and Pb, and led to an increase in the quantities of elements such as: Fe, V, Mn, Co, Ni, Mg, Cr, and As. Most of the Al, Ti, Zr, Zn, W, Ba, and Cu, and a majority of the Fe, V, Mn, and Sn, potentially resulted from a natural source. Samples 24 (Zn, Co, Mn, Fe, V, Cr, Mg, and Ba) and 25 (Ni, Co, Fe, and Mg) contain several numbers of heavy metals, each showing high anomalous concentrations, which are related to anthropogenic sources. The ANOVA model summary reveals the high explanatory power of R2 = 100.00%, indicating that the number of samples used in this study was suffi cient.
The aim of this study was to determine the prevalence of toxoplasmosis, listeriosis and brucellosis in goitered gazelles (Gazella subgutturosa) in Sanliurfa region, Turkey. A total of 82 sera were collected from healthy gazelles and tested for listeriosis, brucellosis and toxoplasmosis by the Osebold Agglutination Test (OAT), Serum Agglutination Test (SAT) and Sabin-Feldman Dye Test (SFDT), respectively. 82 gazelles 5 (6.09%) were seropositive for listeriosis, 23 (28.04 %) for toxoplasmosis and all of them were seronegative for brucellosis. No statistically significant differences were observed between male and female gazelles in the seroprevalences of toxoplasmosis and listeriosis. As a result, the presence of anti-Toxoplasma gondii and Listeria spp. specific antibodies in G. subgutturosa in the region of Sanliurfa was determined.
It has been suggested that some microorganisms may play a role in the etiology or progression of atherosclerotic plaques. The purpose of this study was to assess for the presence of Helicobacter pylori and cytomegalovirus (CMV) DNA using polymerase chain reaction (PCR) technique in vascular-wall specimens obtained during autopsy. Four to 5 mm long samples from 3 different vascular wall specimens (coronary, carotid and abdominal aortas) of 30 patients (23 male, 7 female) were taken for pathologic and microbiologic investigations during autopsy. H. pylori DNA was found in 48.2% atherosclerotic and 19.6% non-atherosclerotic vascular wall specimens, whereas CMV DNA was found in 37.9% atherosclerotic and 32.7% non-atherosclerotic vascular wall specimens. In terms of CMV DNA detection, no statistically significant differences between the atherosclerotic and non-atherosclerotic groups were present (P>0.05). However, there was a statistically significant difference between the atherosclerosis and non-atherosclerotic groups in terms of H. pylori DNA in coronary and abdominal aorta arteries (p = 0.016 and p = 0.0029 respectively) but not in carotid arteries (p= 1.00). In conclusion, the correlation between H. pylori and atherosclerosis could be suggested. These finding warrant further investigation regarding the role of H. pylori in atherosclerosis.
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