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Monodontella giraffae Yorke et Maplestone, 1926 was collected from the biliary duct of liver of a dead giraffe, Giraffa camelopardalis (Linnaeus) from the Tianjin Zoo, Tianjin, China. A redescription of this species was made using light microscopy and scanning electron microscopy (SEM). The lateral alae and the detailed structures of the spicules and genital cone were observed for the first time by SEM. The taxonomic status of the genus is discussed.
Near-infrared spectroscopy (NIRS) can detect soil total phosphorus in agricultural environments. Considering the soil moisture and particle size on total phosphorus prediction, we applied NIRS to the detection of soil samples with different soil moistures and particle sizes. Thus the effect of soil moisture and particle size was analyzed quantitatively and qualitatively. The procedures to remove the effect of soil moisture and particle size on total phosphorus prediction were also described. First, the near-infrared reflectance spectra of soil samples with different soil moistures and particle sizes were obtained and the absorbance values were determined. Next, the original spectra were corrected by using moisture absorbance index (MAI) and hybrid correction to counteract the effects of soil moisture and particle size, respectively. Absorbance of soil samples showed high correlation with soil moisture at wavelengths of 1,450 nm and 1,940 nm. MAI is a tool for normalizing the original spectral data so as to correct for soil moisture. Hybrid correction is based on the superposition of NIR spectra, and a particle size different from that of the original soil samples is generated. This is an effective means of correcting for the effect of soil particle size. Finally, using the corrected absorbance values at eight wavelengths (655, 722, 1,055, 1,255, 1,467, 1,678, 1,890, and 2,246 nm), the soil total phosphorus prediction model was built based on LS-SVM. Compared with the model used for original spectral data, the new model exhibited higher accuracy and stability. Results showed that MAI and hybrid correction are effective for correcting for soil moisture and soil particle size during the prediction of soil total phosphorus.
Subulura (Murisubulura) tanjinensis sp. nov. was collected from the Siberian chipmunks, Eutamias sibiricus (Laxmann) at Jixian County, Tianjin, China. The new species can be distinguished from all species of subgenus Murisubulura by the structures of labia lobes, by having a telamon and by the numbers of caudal papillae.
Two species of Hercostomus absimilis group are described as new to science: Hercostomus gongshanensis sp. nov. and H. mengyangensis sp. nov. A key to the species of the absimilis-group from China is given.
This study investigated the effect of simulated acid rain (SAR) (heavy: pH 2.5; moderate: pH 4.0; and control: pH 5.6) stress on the growth and net photosynthetic rate (Pn) of five tree species, namely Castanopsis sclerophylla, Cinnamomum camphora, Manglietia fordiana, Pinus massoniana, and Elaeocarpus glabripetalus. Results showed variable responses to SAR with different pH values depending on the type of plants. P. massoniana seedlings exhibited significant growth reduction in response to all of the SAR treatments. The net photosynthetic rate of P. massoniana treated by SAR decreased by 20 and 34% under pH 4 and 2.5, suggesting that P. massoniana was susceptible when exposed to acid rain. These results indicate that P. massoniana was the highest sensitivity inhibitory type to SAR and should be protected. However, the growth, chlorophyll content, and Pn of three species (C. sclerophylla, C. camphora, M. fordiana) revealed the following result: moderate acid rain > control > heavy acid rain, suggesting that moderate acid rain promoted photosynthesis and growth to some extent. Among the five species, E. glabripetalus exhibited the highest extent of tolerance to acid rain. The sensitivity of growth and Pn of E. glabripetalus was significantly higher than that of the control, indicating that SAR promoted rather than inhibited its seedling, E. glabripetalus belonging to the promotional type. The stress tolerance of five species of trees to SAR was observed in the following order: E. glabripetalus > C. sclerophylla, C. camphora, M. fordiana > P. massoniana. But exposure to SAR at PH 2.5 to 5.6 did not affect the final mortality of five tree species.
The species of the genus Hercostomus from Guangxi are reviewed. Two species are described as new to science: Hercostomus (Hercostomus) tianeensis sp. nov. and H. (H.) tianlinensis sp. nov.
Background: Anti-angiogenesis therapy that targets VEGF is one of the important treatment strategies in advanced ovarian cancer. However, depending on the pharmaceutical agent, treatment can have undesirable side effects. SEMA4D has recently gained interest for its role in promoting angiogenesis. Here, we try to further understand the mechanism by which SEMA4D promotes angiogenesis in ovarian cancer. Methods: Correlation and western blot assaya were used to detect the relationship between VEGF and SEMA4D in clinical tissues and cells. Vasculogenic mimicry and transwell migration analyses were used to detect the roles of VEGF, SEMA4D and plexin-B1 on vasculogenic mimicry and migration. Vascular density and SEMA4D expression was determined using immunofluorescence staining in clinical tissues of EOC. Western blot was used to detect the expressions of CD31, MMP2 and VE-cadherin. We also analyzed the relationship between VEGF-SEMA4D and malignant tumor prognosis. Results: We found that knockdown of VEGF could suppress SEMA4D expression and that the expressions of VEGF and SEMA4D have a positive correlation in EOC cancer tissues. Vasculogenic mimicry and transwell migration analyses showed that SEMA4D and VEGF have a synergistic effect on the promotion of angiogenesis in A2780 and HUVEC cells. Soluble SEMA4D (sSEMA4D) could promote VM and migration in A2780 and HUVEC cells via the SEMA4D/plexin-B1 pathway, but the effect was not noted in stably transfected shR-plexin-B1 cells. In clinical tissues of EOC, the vascular density and SEMA4D/plexin-B1 expression were higher. When VEGF, SEMA4D and plexin-B1 was knocked down, the expression of CD31, MMP2 and VE-cadherin, which are the markers and initiators of angiogenesis and the epithelial–mesenchymal transition (EMT) process were reduced. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. Conclusions: VEGF and SEMA4D have synergistic effects on the promotion of angiogenesis in epithelial ovarian cancer. Targeting VEGF and the SEMA4D signaling pathway could be important for the therapy for EOC.
In order to effectively extract and maximize the energy from ocean waves, a new kind of oscillating-body WEC (wave energy converter) with moon pool has been put forward. The main emphasis in this paper is placed on inserting the damping into the equation of heaving motion applied for a complex wave energy converter and expressions for velocity potential added mass, damping coefficients associated with exciting forces were derived by using eigenfunction expansion matching method. By using surface-wave hydrodynamics, the exact theoretical conditions were solved to allow the maximum energy to be absorbed from regular waves. To optimize the ability of the wave energy conversion, oscillating system models under different radius-ratios are calculated and comparatively analyzed. Numerical calculations indicated that the capture width reaches the maximum in the vicinity of the natural frequency and the new kind of oscillating-body WEC has a positive ability of wave energy conversion
The aim of this study was to evaluate the effects of dietary supplementation of bamboo leaf extract (BLE) on the growth performance, antioxidant traits, immune function, and lipid metabolism of weaning piglets. After weaning at 21 days, two hundred and forty healthy piglets (Large White × Landrance) were randomly assigned to 4 treatments with 6 pens and 10 piglets per pen. The control group (Ctr) received a maize-soyabean based diet, and the test groups received the control diet plus 0.5%, 1%, or 2% (w/w) BLE, respectively. The experiment was carried out for 5 weeks. At the end of it, average daily feed intake in the 1% BLE group was decreased (p < 0.05). Plasma concentrations of malondialdehyde were decreased with supplementation of 1% and 2% BLE. Immunoglobulin G concentrations and lysozyme activity in plasma were significantly increased in piglets supplemented with BLE. Diets with 1% and 2% BLE increased (p < 0.05) plasma concentrations of low density lipoprotein-cholesterol and reduced (P < 0.01) high density lipoprotein-cholesterol levels. Higher triglyceride concentrations were observed in the 0.5% (P < 0.01) and 2% (p < 0.05) BLE groups. In conclusion, these novel findings demonstrate that supplementation of BLE to the diet improved the antioxidant activity, immune function, and lipid metabolism of weaning piglets.
The objective of this study was to investigate the effect of exogenous selenium (Se) supply (0, 2, 4, 8, 16 μM) on the growth, lipid peroxidation and antioxidative enzyme activity of 100 mM NaCl-stressed melon (Cucumis melo L.) seedlings. Salt stress significantly reduced the growth attributes including stem length, stem diameter, dry weight and increased antioxidative enzyme activity [superoxide dismutase (SOD), peroxidase (POD), catalase (CAT)]. Moreover, the plant exhibited a significant increase in electrolyte leakage and malondialdehyde (MDA) content under NaCl stress. Se supplementation not only improved the growth parameters but also successfully ameliorated the adverse effect caused by salt stress in melon seedlings. However, the mitigation of NaCl-stressed seedlings was different depending on the Se concentration. At lower concentrations (2–8 μM), Se improved growth and acted as antioxidant by inhibiting lipid peroxidation and increasing in SOD and POD enzymes activity under salt stress. At higher concentrations (16 μM), Se exerted diminished beneficial effects on growth. Whereas CAT activity was enhanced. The result indicated that Se supplementation had a positive physiological effect on the growth and development of salt-stressed melon seedlings.
This study analyzes the effects of cold air on catecholamine (CA) levels. The CA levels of patients with cardio-cerebrovascular diseases, healthy people, Wistar rats, and spontaneously hypertensive (SHR) rats were determined before, during, and after cold air activity. The levels of dopamine (DA), norepinephrine (NE), and adrenaline (AD in humans and experimental animals changed in all three periods of cold air activity. The change in DA levels was statistically significant (P<0.05). The DA, NE, and AD levels in the controls and the Wistar rats increased during cold air activity and decreased after cold air activity. The variation in CA levels was not exactly the same between the SHR rats and the cardio-cerebrovascular disease patients. The special meteorological conditions caused by cold air affects CA secretion, which induces the occurrence, development, and outcome of cardio-cerebrovascular diseases. Moreover, the results of the animal experiments could not be directly extrapolated to humans.
Background: Oncogenic Notch1 is known to activate the NF-κB pathway in T cell acute lymphoblastic leukemia (T-ALL) and to up-regulate the transcription of Asb2α, a specificity factor for an E3 ubiquitin ligase complex that plays an important role in hematopoietic differentiation. Therefore, we hypothesize that Notch1 might regulate the NF-κB pathway through Asb2α. Methods: The study involved down-regulation of Notch1 in T-ALL cell lines (CCRFCEM cells and MOLT-4 cells) through treatment with gamma-secretase inhibitor (GSI) as well as the modulation of Asb2 in CCRF-CEM cells and MOLT-4 cells through transduction with lentivirus carrying Asb2 or Asb2-shRNA. Experiments using real-time PCR, western blot and co-immunoprecipitation were performed to evaluate the expression levels of related genes. Cell proliferation and apoptosis were measured while the expression of Asb2 was enhanced or inhibited. Results: Here, we demonstrated for the first time that Notch1 can activate the transcription of Asb2α, which then stimulates activation of NF-κB in T-ALL cells. Asb2α exerts its effects by inducing degradation and dissociation of IκBα from NF-κB in T-ALL cells. Moreover, specific suppression of Asb2α expression can promote apoptosis and inhibit proliferation of T-ALL cells. Conclusion: Notch1 modulates the NF-κB pathway through Asb2α, indicating that Asb2α inhibition is a promising option for targeted therapy against T-ALL.
The Chinese government has made a commitment to achieve a 60-65% reduction of CO2 emissions by 2030 compared with that in 2005. Most provinces are assigned differentiated reduction tasks due to different natural resources endowment, energy consumption structure, and economic developments. Marginal abatement cost (MAC) supplies cost information on regional pollutant reduction processes and should be an important evaluation indicator of policies. In this study, we build a quadratic parametric directional distance function (DDF) to estimate provincial MAC of CO2 emissions in China during 2000-2015. Linear programming is used to solve the parameter estimation problem. Results are as follows: 1) LP method supplies efficient parameter estimation results and obtains 98.33% reliable MACs during the research period. 2) MAC keeps a growing trend for most provinces in 2000-2015. Especially when China enters the New Normal stage in 2012, this growing trend has been accelerated. These trends reveal that MAC gradually becomes a more important indicator to evaluate emission reduction measurements. 3) From a spatial distribution aspect, positive cluster feature has experienced such fluctuations as “apparent rise→significant decline→close to zero.” In this stage, their spatial cluster is close to random distribution state. Spatial heterogeneity turns to being enlarged, especially among provinces at higher MAC range. These evolutionary trends will have important influence on their carbon reduction measure implementing process. Eastern regions should turn more focus on low-carbon technology innovation to push their lowcarbon transformation. For middle and western regions, they should promote their production efficiencyand obtain more technology spillovers from eastern provinces in the future to stimulate their economic growth and low-carbon transformation.
A novel multi-objective discrete particle swarm optimization with elitist perturbation strategy (EPSMODPSO) is proposed and applied to solve the reconfiguration problem of shipboard power system(SPS). The new algorithm uses the velocity to decide each particle to move one step toward positive or negative direction to update the position. An elitist perturbation strategy is proposed to improve the local search ability of the algorithm. Reconfiguration model of SPS is established with multiple objectives, and an inherent homogeneity index is adopted as the auxiliary estimating index. Test results of examples show that the proposed EPSMODPSO performs excellent in terms of diversity and convergence of the obtained Pareto optimal front. It is competent to solve network reconfiguration of shipboard power system and other multi-objective discrete optimization problems
Nitric oxide (NO), a multifunctional gaseous molecule, mediates a variety of responses to biotic and abiotic stresses. The effects of exogenous NO on rice (Oryza sativa cv. ‘Zhonghua 11’) growth under mercuric chloride (HgCl2) stress were investigated. The results showed that 60 lM Hg significantly inhibited the root elongation of rice plantlets after seed germination. While 100 lM or 200 lM sodium nitroprusside (SNP, a donor of NO) could increase the root length by attenuating the effects of 2,3,5-triiodobenzoic acid (TIBA) and Hg, which indicated the role of NO in auxin transport-promoting in roots. On the other hand, SNP decreased the absorption and transportation of Hg in roots and shoots of rice seedlings at five-leaf stage. Moreover, the levels of superoxide radical (O2-) and hydrogen peroxide (H2O2) in leaves were also decreased significantly. However, the activities of antioxidant enzymes were not enhanced by SNP. Moreover, NO promoted the growth of rice plantlets under Hg stress even when superoxide dismutase (SOD, EC 1.15.1.1) or catalase (CAT, 1.11.1.6) activity was inhibited by diethyldithiocarbamate (DDC, an inhibitor of SOD) or 3-amino-1,2,4- triazole (AT, an inhibitor of catalase), respectively. These results confirmed that NO could act as the direct quencher of O2- and then prevent the oxidative damage caused by Hg ion in leaves.
We explored the possibility of using allelochemicals from terrestrial plants to solve the problem of cyanobacterial blooms in eutrophicated shallow lakes. The highly effective inhibitory allelochemicals of purslane (Portulaca oleracea L.) and purslane seeds were extracted and purified through a series of procedures, and the chemical components of the purified extracts were analyzed by gas chromatographymass spectrometry (GC-MS). The extracts contained 12 fatty acids and three phenolic acids, of which nine compounds were found to be inhibitors of harmful cyanobacteria. The inhibitory ratios of the purified extracts of purslane and purslane seeds on M. aeruginosa were 97.4 and 81.6%, respectively, when the cyanobacterial were exposed under 15.0 mg/L of extracts. The purified extracts exhibited significant anticyanobacterial activities, the organic acids that may contribute to the allelopathic effects of the purslane and purslane seeds on M. aeruginosa.
Seven species of Cylicocyclus Ihle, 1922 (Nematoda: Strongylidae) were collected from donkeys from Henan Province, China. Five samples of each species were selected for sequencing. Sixteen different internal transcribed spacer (ITS) sequences representing the seven species of Cylicocyclus were obtained. Sequence differences in the first internal transcribed spacer (ITS-1) among species was lower than that of the second internal transcribed spacer (ITS-2). Phylogenetic analyses were conducted using the combined ITS-1 and ITS-2 data sets from the present study and using reference sequences from the GenBank database. The MP and ML trees were similar in topology. The phylogenetic trees were divided into two clades. Clade I included 8 species of Cylicocyclus; within this group, Cylicocyclus leptostomus (Kotlan, 1920) is nested between different samples of Cylicocyclus ashworthi (LeRoux, 1924), suggesting C. ashworthi may represent a species complex. Clade II included Cylicocyclus elongatus (Looss, 1900) and Cylicocyclus ultrajectinus (Ihle, 1920); however, these two species always clustered with the comparative species (Petrovinema poculatum (Looss, 1900) and Poteriostomum imparidentatum Quiel, 1919), suggesting that C. elongatus and C. ultrajectinus represent members of other genera.
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