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High efficiency method to obtain supercoiled DNA with a commercial plasmid purification kit

100%

Carbone A., Fioretti F. M., Fucci L., Ausio J., Piscopo M.

Acta Biochimica Polonica

2012

tom 59

nr 2

Supercoiled state corresponds to the active form for plasmid applications. The relaxed circular form of plasmids is often inactive or poorly active. To obtain significant amounts of almost fully supercoiled DNA, we modified the standard protocol of a commercially; available Qiagen plasmid purification kit. Our changes led to isolation of almost 100% of the plasmids in the supercoiled state. The modified protocol was used to purify different plasmids with consistent results. The purified plasmids maintain supercoiled state for about two months. The modified protocol is very advantageous because it allows easy DNA production with high degree of supercoiled form at low cost.

Protamine-like proteins analyses as emerging biotechnique for cadmium impact assessment on male mollusk Mytilus galloprovincialis (Lamarck 1819)

58%

Piscopo M., Trifuoggi M., Notariale R., Labar S., Troisi J., Giarra A., Rabbito D., Puoti R., de Benedictis D., Brundo M. V., Basile A., Good K. V., Ausio J., Guerriero G.

Acta Biochimica Polonica

2018

tom 65

nr 2

Protamine-like proteins have bactericidal activity. The first evidence in Mytilus galloprovincialis

58%

Notariale R., Basile A., Montana E., Romano N. C., Cacciapuoti M. G., Alberti F., Gesuele R., De Ruberto F., Sorbo S., Tenore G. C., Guida M., Good K. V., Ausio J., Piscopo M.

Acta Biochimica Polonica

2018

tom 65

nr 4

Ograniczanie wyników

3 Acta Biochimica Polonica

3 Ausio J.

3 Piscopo M.

2 Basile A.

2 Good K. V.

2 Notariale R.

1 Alberti F.

1 Brundo M. V.

1 Cacciapuoti M. G.

1 Carbone A.

1 De Ruberto F.

1 Fioretti F. M.

1 Fucci L.

1 Gesuele R.

1 Giarra A.

1 Guerriero G.

1 Guida M.

1 Labar S.

1 Montana E.

1 Puoti R.

1 Rabbito D.

1 Romano N. C.

1 Sorbo S.

1 Tenore G. C.

1 Trifuoggi M.

1 Troisi J.

1 de Benedictis D.

2 2018

1 2012

Wyniki wyszukiwania

High efficiency method to obtain supercoiled DNA with a commercial plasmid purification kit

Protamine-like proteins analyses as emerging biotechnique for cadmium impact assessment on male mollusk Mytilus galloprovincialis (Lamarck 1819)

Protamine-like proteins have bactericidal activity. The first evidence in Mytilus galloprovincialis