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1

Response of stomatal density and bound gas exchange in leaves of maize to soil water deficit

100%
Zhao W., Sun Y., Kjelgren R., Liu X.
Acta Physiologiae Plantarum
|
2015
|
tom 37
|
nr 01
Stomatal behavior in response to drought has been the focus of intensive research, but less attention has been paid to stomatal density. In this study, 5-week-old maize seedlings were exposed to different soil water contents. Stomatal density and size as well as leaf gas exchange were investigated after 2-, 4- and 6-week of treatment, which corresponded to the jointing, trumpeting, and filling stages of maize development. Results showed that new stomata were generated continually during leaf growth. Reduced soil water content significantly stimulated stomatal generation, resulting in a significant increase in stomatal density but a decrease in stomatal size and aperture. Independent of soil water conditions, stomatal density and length in the trumpeting and filling stages were greater than in the jointing stage. Irrespective of growth stage, severe water deficit significantly reduced stomatal conductance (Gs), decreasing the leaf transpiration rate (Tr) and net photosynthetic rate (Pn). Stomatal density was significantly negatively correlated with both Pn and Tr but more strongly with Tr, so the leaf instantaneous water use efficiency (WUEi) correlated positively with stomatal density. In conclusion, drought led to a significant increase in stomatal density and a reduction in stomatal size and aperture, resulting in decreased Pn and Tr. Because the negative correlation of stomatal density to Tr was stronger than that to Pn, leaf WUEi tended to increase.
2
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Prediction of moisture content uniformity using hyperspectral imaging technology during the drying of maize kernel

88%
Huang M., Zhao W., Wang Q., Zhang M., Zhu Q.
International Agrophysics
|
2015
|
tom 29
|
nr 1
Moisture content uniformity is one of critical parameters to evaluate the quality of dried products and the drying technique. The potential of the hyperspectral imaging technique for evaluating the moisture content uniformity of maize kernels during the drying process was investigated. Predicting models were established using the partial least squares regression method. Two methods, using the prediction value of moisture content to calculate the uniformity (indirect) and predicting the moisture content uniformity directly, were investigated. Better prediction results were achieved using the direct method (with correlation coefficients RP = 0.848 and root-mean-square error of prediction RMSEP = 2.73) than the indirect method (RP = 0.521 and RMSEP = 10.96). The hyperspectral imaging technique showed significant potential in evaluating moisture content uniformity of maize kernels during the drying process.
3

Recombinant ferritin protects mice against challenge with Echinococcus granulosus?

88%
Wang Y., Li Z., Li Z., Bo Y., Zhao W.
Acta Parasitologica
|
2009
|
tom 54
|
nr 4
335-340
Ferritin is well known as the main intracellular iron storage protein in both prokaryotes and eukaryotes, keeping it in a soluble and non-toxic form, though the role of ferritin as a vaccine candidate in echinococcosis has not yet been delineated. Through our study, ferritin was cloned from Echinococcus granulosus and expressed in Escherichia coli. The recombinant E. granulosus ferritin (rEgferritin) has a molecular weight of 19 kDa and could be recognized by anti-mice serum in Western blotting. The specific antibody was detected by enzyme-linked immunosorbent assay. Mice vaccinated with rEgferritin and challenged intraperitoneally with E. granulosus protoscoleces revealed significant protective efficacy up to 85.6%, compared with the control group. Thus, rEgferritin could be a promising candidate as an effective vaccine to prevent the infection of echinococcosis.
4

Transcriptome analysis reveals differentially expressed genes associated with propamocarb response in cucumber (Cucumis sativus L.) fruit

76%
Wu P., Qin Z., Zhao W., Zhou X., Wu T., Xin M., Guo Q.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 08
High-throughput tag-sequencing (Tag-seq) from Illumina analysis, which is based on the Solexa Genome Analyzer platform, was applied to analyze the gene expression profiling of propamocarb (PM) treatment and control in cucumber fruit. Approximately 3.6 million complete clean sequence tags at PM treatment or control library were obtained with approximately 0.1 million distinct clean tag sequences. Approximately 41.79–43.15 % of the distinct clean tags were mapped unambiguously to the unigene database, and 32.54–33.46 % of the distinct clean tags were mapped to the cucumber genome database. The profiling analysis of the differentially expressed genes revealed the up-regulation of 546 genes and the downregulation of 185 genes with PM response. Furthermore, the differentially expressed genes mainly linked to pesticide detoxication, response to stress/stimulus, transporter/ signaling, and some important transcription factors. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) and reverse transcription polymerase chain reaction (RT-PCR) using 16 genes independently verified the tag-mapped results. The present study reveals the comprehensive mechanisms of PM response in cucumber fruit.
5

Spatial spillover effect and sources of city-level haze pollution in China: a case study of Guangdong Provinces

76%
Liao S., Wang D., Liang Z., Xia C., Guo H., Zhao W.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 5
6

Melatonin modulates the effects of diethylstilbestrol [DES] on the anterior pituitary of the female Wistar rat

76%
Zhao W., Shi Z., Yuan F., Li G., Sun Y., Zhang Y., Wang Z.
Folia Histochemica et Cytobiologica
|
2010
|
tom 48
|
nr 2
278-283
7

Microbiota and chemical compounds in fermented Pinelliae Rhizoma (Banxiaqu) from different areas in the Sichuan Province, China

76%
Shu B., Ying J., Wang T., Xia M., Zhao W., You L.
Polish Journal of Microbiology
|
2019
|
tom 68
|
nr 1
This study focused on the microbiota and chemical compounds of the fermented Pinelliae Rhizoma produced in Longchang (LC), Zizhong (ZZ) and Xindu (XD), in Sichuan Province (China). High-throughput sequencing was used to analyze the microbiota. GC-MS and LC-MS were used to detect the compounds produced during the three different Pinelliae Rhizoma fermentation processes. The bacteria and fungi of the three fermented Pinelliae Rhizoma differed substantially, with the bacterial content mainly composed of the Bacillus genus, while the common fungi were only included in four OTUs, which belong to three species of Eurotiomycetes and Aspergillus cibarius. 51 volatile compounds were detected; they varied between LC, XD, and ZZ fermented Pinelliae Rhizoma. C10 and C15 terpenes were most frequently detected, and only curcumene and β-bisabolene were detected in the three fermented Pinelliae Rhizoma. 65 non-volatile compounds were detected by LC-MS, most were of C16, C18, C20, C21 and C22 structures. Cluster analysis showed more similarity between LC and XD fermented Pinelliae Rhizoma with regards to volatile compound content, but more similarity between the XD and ZZ fermented Pinelliae Rhizoma for non-volatiles. Moreover, no correlation between geographical distance and microflora or compounds of fermented Pinelliae Rhizoma was observed. These results showed that hundreds of compounds are produced by the natural mixed fermentation of Pinelliae Rhizoma, and may mostly relate to the microorganisms of five species.
8

Functional characterization of a grape heat stress transcription factor VvHsfA9 in transgenic Arabidopsis

76%
Li Z., Tian Y., Zhao W., Xu J., Wang L., Peng R., Yao Q.
Acta Physiologiae Plantarum
|
2015
|
tom 37
|
nr 07
Plant heat stress transcription factor (HSF) family consists of a group of proteins participating in various signal transduction processes. However, only little information regarding HSF genes is available in grape. In this study, we reported the functional analysis of VvHsfA9 (Vitis vinifera HsfA9) in Arabidopsis. VvHsfA9 and HaHsfA9 (Helianthus annuus HsfA9) shared 34.72 % identity over the whole amino acid sequence. We discovered that the C-terminal part (VvHsfA9262–340) is required for the transcriptional activation activity of VvHsfA9. Overexpression of VvHsfA9 in Arabidopsis confers enhanced seed germination under unstressed conditions and late-flowering phenotypes. However, no other stress tolerance was observed during post-germination stage. These results suggest that VvHsfA9 functions as a seedspecific transcription factor in positive modulation of seed germination and may negatively regulate flowering time of plants.
9
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Isolation of biosynthesis related transcripts of 2,3,5,4'-tetrahydroxy stilbene-2-O-beta-D-glucoside from Fallopia multiflora by suppression subtractive hybridization

64%
Zhao W., Sheng S., Liu Z., Lu D., Zhu K., Li X., Zhao S., Yao Y.
Acta Societatis Botanicorum Poloniae
|
2014
|
tom 83
|
nr 2
2,3,5,4'-tetrahydroxy stilbene-2-O-ß-D-glucoside (THSG) exerts multiple pharmacodynamic actions, found in Fallopia multiflora, but the biosynthesis pathway of THSG is still unclear. To clear this ambiguity, we constructed suppression subtractive hybridization (SSH) libraries to screen the genes involved in THSG biosynthesis from two F. multiflora varieties, which vary significantly in THSG content. Twelve non-redundant differentially expressed sequence tags were obtained and the full lengths of 4 unreported fragments were amplified by rapid amplification of cDNA ends. We totally got 7 fulllength transcripts, and all of them were aligned to the transcriptome and digital gene expression tag profiling database of four F. multiflora tissues (root, stem and leaf from Deqing F. multiflora and another root from Chongqing F. multiflora; data unpublished) using local BLAST. The results showed that there was a significant, organ specific difference in the expression of fragments and full-length sequences. All the sequences were annotated by aligning to nucleotide and protein databases. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that THSG biosynthesis was correlated with multiple life activities.
10

Effects of inorganic seed aerosol on the formation of nitrogen-containing organic compounds from reaction of ammonia with photooxidation products of toluene

64%
Xu J., Huang M., Feng Z., Cai S., Zhao W., Hu C., Gu X., Zhang W.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 1
11

Plant density influences fiber sucrose metabolism in relation to cotton fiber quality

64%
Meng Y., Lv F., Zhao W., Chen J., Zhu L., Wang M., Chen B., Zhou Z.
Acta Physiologiae Plantarum
|
2016
|
tom 38
|
nr 05
12

Gene expression profile of Arabidopsis under sodium bisulfite treatment by oligo-microarray analysis

64%
Zhu B., You S.-H., Han H.-J., Fu X.-Y., Zhao W., Gao J.-J., Xue Y., Peng R.-H., Yao Q.-H.
Acta Physiologiae Plantarum
|
2015
|
tom 37
|
nr 08
Low concentrations of sodium bisulfite (NaHSO3) can enhance some plant photosynthetic efficiency. To investigate the growth process of Arabidopsis seedlings in response to NaHSO3 treatment and to obtain a better understanding the mechanism of NaHSO3 treatment toward Arabidopsis, microarray experiments on Arabidopsis thaliana were performed in the present study. Seedlings growth and physiological responses of Arabidopsis to NaHSO3 were investigated. The results indicated that the NaHSO3 response was related to photosynthesis pathways and reactive oxygen species (ROS) accumulation. Our findings could provide valuable gene resources and theoretical information for understanding the physiological responses and dissecting the NaHSO3 response pathways in higher plants.
13

Comparative analysis on mRNA expression and DNA methylation status of SYCE1 gene in testes of yaks and cattleyaks

64%
Luo H., Yi C., Wu S., Yuan M., Li P., Gou Q., Zhao W., Cai X.
Animal Science Papers and Reports
|
2020
|
tom 38
|
nr 1
14

Isolation from Proteus mirabilis of a novel class I 5-enopyruvylshikimate-3-phosphate synthase with glyphosate tolerance in transgenic Arabidopsis thaliana

64%
Tian Y.-S., Jin X.-F., Xu J., Han J., Zhao W., Fu X.-Y., Peng R.-H., Wang R.-T., Yao Q.-H.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 02
Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Proteus mirabilis was identified and isolated. Furthermore, the transgenic Arabidopsis with the novel aroA gene was obtained to confirm the potential of the novel aroA gene in developing glyphosate-resistant crops.
15
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Survival and effect of exopolysaccharide-producing Lactobacillus plantarum YW11 on the physicochemical properties of ice cream

64%
Zhang J., Zhao W., Guo X., Guo T., Zheng Y., Wang Y., Hao Y., Yang Z.
Polish Journal of Food and Nutrition Sciences
|
2017
|
tom 67
|
nr 3
Ice cream was prepared with exopolysaccharide (EPS)-producing Lactobacillus plantarum YW11 by direct inoculation (DI), addition of prefermented skim milk (FSM), or addition of the lyophilized powder of the YW11 strain (LP) into the ice cream mix. After 4 weeks of storage, viable counts of the YW11 strain decreased in all groups by 0.8–1.61 log cfu/g. Furthermore, ice cream made using the LP method showed the highest survival rate. The ice cream processing and storage conditions also affected the YW11 strain’s tolerance to acid and bile, with a decrease in survival rate of 38.8–63.2% and 10.8–51.8%, respectively. The degree of impact on the viability of strain YW11 was hardening>aging>freezing>storage (p<0.05). The YW11 strain produced a ropy EPS (up to 4.84 mg/g) in the ice cream mix made using the DI and FSM methods; it was present as a fine porous matrix as observed by Cryo-SEM. Formation of the EPS together with changes in the pH of the ice cream mix caused increased viscosity (up to 131.0 mPa·s), overrun and meltdown, decreased destabilization of fat, and firmness of ice cream. Hydrocarbons, ketones, and benzenes were found to be the major volatiles in the fermented ice cream samples, which also had decreased levels of dodecane, characterized by the smell of dirt.
16

Effect of maternal dietary supplementation with phytosterol esters on muscle development of broiler offspring

52%
Wang L., Zuo X., Zhao W., Zhou G., Luo L., Yang K., Shu G., Wang S., Gao P., Zhu X., Jiang Q., Wang L.
Acta Biochimica Polonica
|
2020
|
tom 67
|
nr 1
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