Wyniki wyszukiwania

1

Numerical investigation of temperature distribution of thermal discharge in a river-type reservoir

100%

Wang S., Chen F., Zhang W.

Polish Journal of Environmental Studies

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2019

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tom 28

|

nr 5

A 3-D thermal discharge numerical model based on the Navier-Stokes equation, the k–ε turbulence model, and the temperature diffusion-controlled equation was developed to simulate the 3-D distribution of thermal discharge along a river-type reservoir under different discharge conditions, hydrological conditions and reservoir water levels. Results showed that the thermal discharge from the power plant would have a smaller effect on a deep-water reservoir. Neither 1ºC, 2ºC nor 3ºC isotherm appear in any scenario conditions. For dam water depths of 155 m and 175 m, a small envelope area of 0.05ºC isothermal line was predicted. The isothermal lines of 0.05ºC and 0.1ºC covered a small area in all scenario conditions. The temperature increase 500 m downstream of the discharge point was predicted to be less than 0.05ºC during dry seasons. The predicted stability time of the temperature increase in each layer was 20 days. It evidences the thermal impact intensity and the extent is different under three scenario conditions. The predicted space–time distribution of the cooling water and the temperature increase provide scientific bases for designing water intake and water management. In the future, the influence of thermal discharge on water quality and aquatic organisms of the reservoir will be discussed based on the simulation results of this study, that is, the variation law of water temperature caused by thermal discharge.

2

Comparative and evolutionary analysis of new variants of psi-gliadin genes from three A-genome diploid wheats

100%

Zhuang Q., Zhang Z., Chen F., Xia G.

Journal of Applied Genetics

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2012

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tom 53

|

nr 2

A genomic polymerase chain reaction (PCR) cloning strategy was applied to isolate ω-gliadin sequences from three A-genome diploid wheats (Triticum monococcum, T. boeoticum and T. urartu). Amplicon lengths varied from 744 and 1,044 bp, and those of the corresponding deduced mature proteins from 248 to 348 residues. The primary structure of the deduced polypeptides comprised a short N- and C-terminal conserved domain, and a long, variable repetitive domain. A phylogenetic analysis recognised several clades: the first consisted of three T. aestivum sequences; the second and the third two T. boeoticum and six T. monococcum sequences; and the rest four T. urartu and three T. aestivum sequences. Among the functional (non-pseudogene) ARQ/E-type ω-gliadin sequences, two were derived from T. boeoticum and three from T. monococcum; one of the latter sequences appeared to be a chimera originating via illegitimate recombination between the other two T. monococcum sequences. None of the 12 intact ω-gliadin sequences contained any cysteine or methionine residues. We discussed the variation and evolution of A-genome ω-gliadin genes.

3

Transcriptome of wax apple (Syzygium samarangense) provides insights into nitric oxide-induced delays of postharvest cottony softening

88%

Chen F., Hao Y., Yin Z., Wu G., Jiang X.

Acta Physiologiae Plantarum

|

2017

|

tom 39

|

nr 12

4

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Climatic signals in tree rings of Juniperus turkestanica in the Gulcha River Basin (Kyrgyzstan), reveals the recent wetting trend of high Asia

88%

Chen F., He Q., Bakytbek E., Yu S.-L., Zhang R.-B.

Dendrobiology

|

2015

|

tom 74

Drought variability of Kyrgyzstan is an important component of climate change of high Asia. Long-term information about the history of drought is, however, limited because the instrumental records are short. Here, we developed three chronologies for juniper trees (Juniperus turkestanica) under different microsite conditions in the Gulcha River Basin, Kyrgyzstan. The three chronologies (GUL, GUR and GUD) were compared with climate data which covered the study area. Growth of the GUL site correlates positively with May–June precipitation of the current growing season and September precipitation of the previous year, but negatively with temperatures of prior July, current May and July. Growth of the GUR site correlates positively with precipitation of current May and September, and negatively with temperature of current June and July. Ring width at GUD site is negatively correlated with temperature of the current May and July, and positively correlated with precipitation of prior December and current September. Response analysis shows that water availability is the main factor limiting the radial growth of juniper trees at the GUL and GUD sites. Based on the relationships derived from climate response analyses, the potential of tree-ring chronologies from this species to provide drought reconstructions in the Gulcha River Basin has been established. The GUL chronology and other moisture sensitive tree-ring series from high Asia capture the recent wetting trend. The records contribute to a growing tree-ring network for high Asia, including sites in China, Kyrgyzstan, Pakistan and Tajikistan.

5

A study on the fundamental factors determining the efficacy of siRNAs with high C-G contents

88%

Liao J. Y., Yin J. Q., Chen F., Liu T.-G., Yue J.-C.

Cellular and Molecular Biology Letters

|

2008

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tom 13

|

nr 2

283-302

Although there are many reports about the efficacy of siRNAs, it is not clear whether those siRNAs with high C/G contents can be used to silence their target mRNAs efficiently. In this study, we investigated the structure and function of a group of siRNAs with high C/G contents. The results showed that single siRNAs against the Calpain, Otoferlin and Her2 mRNAs could induce different silencing effects on their targets, suggesting that the accessibility to target sequences influences the efficacy of siRNA. Unexpectedly, a single siRNA could target its cognate sequence in the 3’UTR of EEF1D or the 5’UTR of hTRF2 or CDC6. Their interaction induced different modes of gene silencing. Furthermore, the introduction of mutations into the 3’ end of the passenger strand showed that the position and number of mutated nucleotides could exert some influence on the efficacy of siRNA. However, these mutations did not completely block the passenger strand from exerting its RNAi effect. Interestingly, our findings also indicated that the target mRNA might play essential roles in maintaining or discarding the guide strand in RISCs. Thus, the conclusion could be drawn that favorable siRNA sequences, accessible target structures and the fast cleavage mode are necessary and sufficient prerequisites for efficient RNAi.

6

Comparative analysis of natural cold acclimation and deacclimation of two Magnolia species with different winter hardiness

88%

Yang Y., Jia Z., Chen F., Sang Z., Ma L.

Acta Physiologiae Plantarum

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2015

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tom 37

|

nr 07

Winter injury is frequently observed in the rare species Magnolia wufengensis L.Y. Ma et L.R. Wang, but rarely in Magnolia denudata Desr in northern China. To improve the understanding of the causes for poor winter hardiness of M. wufengensis, the physiological and biochemical processes of M. denudata and M. Wufengensis during natural cold acclimation and deacclimation were investigated and compared. In early cold acclimation, the growth cessation of M. wufengensis was later than that of M. denudata, and the leaf senescence was also slower in M. wufengensis. Both the bud dormancy level and shoot freezing tolerance were lower in M. wufengensis throughout cold acclimation and deacclimation. A significant development in bud dormancy level and shoot freezing tolerance appeared long after growth cessation for M. wufengensis but soon for M. denudata. The increase rates of dormancy level and freezing tolerance during cold acclimation were lower in M. wufengensis, while the corresponding decrease rates of these two parameters during deacclimation were also lower in M. wufengensis. The interspecific differences in freezing tolerance highly correlated with the dehydration and accumulation of proline and certain soluble sugars. We conclude that weaker mid-winter cold hardiness and slower cold acclimation should be the reasons for poorer winter hardiness in M. wufengensis.

7

Spatio-temporal variability of microbial eukaryotic community composition in a large shallow subtropical lake, assessed by 18SrRNA gene sequences

88%

Chen M., Chen F., Zhao B., Qinglong L. W., Kong F.

Acta Protozoologica

|

2009

|

tom 48

|

nr 3

245-264

8

Photosystem II activity in the leaves and assimilative branches of alhagi sparsifolia Shap. under brief elevated temperature

88%

Li L., Li X., Xu X., Lin L., Chen F.

Acta Physiologiae Plantarum

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2014

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tom 36

|

nr 07

Leaves and assimilative branches are crucial to the life cycle of Alhagi sparsifolia Shap. that is one of the main shrubs in the southern fringe of the Taklamakan desert. They reduce photosynthesis at midday during summer and exhibit different degrees of adaptability to severe environments. We investigated the changes in the PSII activity of leaves and assimilative branches of under elevated temperature and whether they have the same response and level of tolerance to different temperatures, thus to understand the roles of leaves and assimilative branches of A. sparsifolia in global warming. In this study, the kinetics of induced chlorophyll a fluorescence under heat-stress-induced inhibition of photosystem II in leaves and assimilative branches of A. sparsifolia after 15 min of treatment was investigated. The results show that both the activity and density of reaction centers of leaves are higher than assimilative branch at 35–48°C, and both in the leaves and assimilative branches were decreased above 45°C, the assimilative branches adapted better to the severe environment in terms of light energy transfer, light usage efficiency, and electron transport at 52°C. Assimilative branches had better adaptability to elevated temperature than leaves of A. sparsifolia suggesting that assimilative branches might be more adaptive to severe environment.

9

Nitrogen removal from piggery wastewater within a sequencing batch reactor using pseudomonas putida

88%

Yang Y., Chen F., Xie L., Xiong J., Hu K.

Polish Journal of Environmental Studies

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2017

|

tom 26

|

nr 2

This work evaluates the efficiency of ammonium removal from piggery wastewater using a sequencing batch reactor (SBR) inoculated with a newly isolated strain of Pseudomonas putida HJH1. The strain HJH1 not only could survive and remove up to 72.4 mg/L NO₂⁻-N under aerobic conditions, but it also has good performance for simultaneous nitrification and denitrification (SND) with no nitrite accumulation. The SBR system was able to consistently remove: 1) 76.4-100% ammonium nitrogen and 71.4-100% COD from artificial wastewater in stage 1, and 2) 89.2-99.1% ammonium nitrogen and 82.4-100% COD from piggery wastewater in stage 2. During the whole operation, the strain HJH1 predominated in the SBR all the time to function together with other bacteria. Results indicated that the SBR system inoculated with Pseudomonas putida HJH1 can efficiently remove ammonium nitrogen from piggery wastewater, thereby having potential applications for future nitrogen removal.

10

Expiration date prediction of biocontrol agent prepared with Bacillus subtilis B579 using the accelerated aging method

88%

Zheng Y., Jiao X., Chen F., Wang X., Wang M.

Polish Journal of Microbiology

|

2016

|

tom 65

|

nr 4

The expiration date of biopesticidal products is an essential feature of their use and storage. In the present work, the expiration date of biocontrol agent was predicted using the accelerated aging method. The available bacteria in Bacillus subtilis B579 biocontrol agent were 3.7 ± 0.2 × 10¹¹ CFU/g. It is calculated that the expiration date of the agent was about 17 months at 25°C. During this period, the available bacteria retained more than 90% of the value in the initial product. Thus, this work suggests the expiration date of biocontrol agents composed with spores could be estimated using the accelerated aging method.

11

Cr (VI) removal from aqueous solution using starch and sodium carboxymethyl cellulose-coated Fe and Fe /Ni nanoparticles

76%

Wang J., Ji B., Shu Y., Chen W., Zhu L., Chen F.

Polish Journal of Environmental Studies

|

2018

|

tom 27

|

nr 6

Starch and sodium carboxymethyl cellulose-coated Fe and Fe /Ni nanoparticles were synthesized and their Cr (VI) removal capabilities were evaluated and compared. We found that starch and sodium carboxymethyl cellulose-coated nanoscale zero-valent iron-nickel (SS-nZVI-Ni) showed the better Cr (VI) removal performance. The effect of acidic conditions on Cr(VI) removal by SS-Nzvi-Ni revealed that the Cr (VI) removal efficiency by SS-nZVI-Ni reached the maximum of 95.70% at pH = 2. The effect of different initial Cr (VI) concentrations showed that SS-nZVI-Ni performed well at a high Cr(VI) concentration. Langmuir-Hinshelwood first-order kinetic model could describe the reduction process well. SEM images revealed that SS-nZVI-Ni had a large surface area, which discarded the problem of aggregation. XRD and XPS analysis of SS-nZVI-Ni showed that SS-nZVI-Ni and Cr (III) formed an alloy on the surface of SS-nZVI-Ni after the reaction. The study provides an option for practical application of SS-nZVI-Ni in Cr (VI) removal.

12

Cloning, characterization and overexpression of the complete open reading frame of the lateral shoot-inducing factor from Jatropha Curcas

76%

Zong H., Wang S., Ouyang C., Wang W., Li L., Chen F.

Acta Physiologiae Plantarum

|

2012

|

tom 34

|

nr 1

Shoot branching is considered a key factor that influences plant architecture and productivity. In the present study, the open reading frame encoding lateral shootinducing factor, named JLIF, was cloned from Jatropha curcas L. JLIF encoded a 214 amino acid polypeptide and contained a strongly conserved QALGGH motif in zincfinger domain which is the typical motif of the SUP protein family. Sequence and phylogenetic analysis indicated that JLIF had very high similarity (97.67%) to the LIF gene in petunia, but showed no significant similarity to other known SUP proteins except for the conserved motifs. Taken together, we presumed JLIF was the ortholog of LIF protein, and both appeared to belong to a novel subfamily of the SUP proteins. Semi- quantitative RT-PCR analysis revealed JLIF was abundant in stems and petioles, weakly expressed in leaves and seeds, but absent in roots. Subsequently, the JLIF gene was introduced into tobacco under the control of the Cauliflower Mosaic Virus (CaMV) 35S promoter. PCR analysis and Southern blot hybridization were performed to confirm if JLIF was stably integrated into the tobacco genome. GUS activity analysis and RTPCR revealed the location of the overexpressed JLIF. Overexpression of JLIF in transgenic tobaccos resulted in reduced plants height and shortened internodes in vitro.

13

The heterogeneity of cell subtypes from a primary culture of human amniotic fluid

76%

Zhang S., Geng H., Xie H., Wu Q., Ma X., Zhou J., Chen F.

Cellular and Molecular Biology Letters

|

2010

|

tom 15

|

nr 3

424-439

Heterogeneous human amniotic fluid contains various cell types. Herein, we report on the possibility of simultaneously isolating three subtypes of cells from one primary culture. Using a stainless steel instrument named a colony poculum, two of the three cell subtypes could be efficiently cultured, and these were further characterized. The results indicated that these two cell subtypes had different morphologies and were characterized by different cell marker expression profiles, including the differential expression of CD105, CD117 and EBAF. Furthermore, their gene expression array data revealed their different gene expression profiles. Although both cell types expressed several embryonic stem cell-specific markers, they were non-tumorigenic in vivo. This paper not only provides new insight into the heterogeneity of human amniotic fluid, it also presents a simple yet efficient cell isolation method. These results will contribute to the thorough investigation of the properties and potential future applications of human amniotic fluid-derived cells.

14

Effects of low nitrogen and drought stresses on proline synthesis of Jatropha curcas seedling

76%

Wang W. G., Li R., Liu B., Li L., Wang S. H., Chen F.

Acta Physiologiae Plantarum

|

2011

|

tom 33

|

nr 5

Proline is one of the most important osmoregulatory solutes subjected to osmotic stresses. In this study, low nitrogen supply suppressed the dry biomass, leaf area, and proline biosynthesis of the seedlings of the energy plant Jatropha curcas, which could grow in poor, dry soil. Low-nitrogen stress induced JcP5CS mRNA expression and decreased the activity of P5CS enzyme and the content of free proline in leaves of J. curcas seedlings. When the seedlings grown in low-nitrogen conditions were suddenly exposed to PEG-6000 (-1.6 MPa) stress, the expression of JcP5CS gene was highly induced, and both the activity of P5CS and the content of free proline increased and maintained at high levels to mitigate the impact of drought stresses. This may be one of the reasons why J. curcas could adapt to poor and drought conditions.

15

Shelf life extension of shrimp (white) using modified atmosphere packaging

76%

Kalleda R. K., Han I. Y., Toler J. E., Chen F., Kim H. J., Dawson P. L.

Polish Journal of Food and Nutrition Sciences

|

2013

|

tom 63

|

nr 2

Wild caught shrimp can have a shortened shelf life compared to farm raised shrimp due to handling and on-ship limitations. The loss of freshness in shrimp is partly due to autolytic reactions caused by endogenous enzymes such as polyphenol oxidase. The objective of this study was to determine the effect of sulfi tes combined with Modified atmosphere packaging (MAP) on the shelf life of non-frozen shrimp. Fresh South Atlantic white shrimp were subjected to one of four treatments, no bisulfite rinse-air packaged, 1.25% bisulfite rinse-air packaged, 1.25% bisulfite rinse-MAP (60% CO2, 18% O2, 22% N2) and 1.25% bisulfite rinse-MAP (36% CO2, 64%N2). The quality and freshness of shrimp was measured by determining total aerobic bacterial populations, package gas headspace analysis, shrimp volatiles (GC-MS), meat pH, nucleotide degradation, and visual analysis. Fresh non-frozen shrimp treated with a combination of sulfites and MAP maintained the shelf life of fresh shrimp up to 10 days while shrimp in non-MAP without sulfite and non-MAP with sulfite developed black spots within 2 and 6 days, respectively. Both MAP treatments slowed bacterial growth while the MAP with high CO2 and with O2 was more effective in preventing off odors and nucleotide degeneration.

16

The response of Mo-hydroxylases and abscisic acid to salinity in wheat genotypes with differing tolerances

64%

Xu H., Zhai J., Liu Y., Cheng X., Xia Z., Chen F., Cui D., Jiang X.

Acta Physiologiae Plantarum

|

2012

|

tom 34

|

nr 5

The differential responses of the wheat cultivars Shi4185 and Yumai47 to salinity were studied. The higher sensitivity of Yumai47 to salinity was linked to a greater growth reduction under salt stress, compared to more salt-tolerant Shi4185. Salinity increased the Na⁺, proline and superoxide anion radical (O₂⁻) contents in both cultivars. Leaf Na⁺ content increased less in the more salttolerant cultivar Shi4185 than salt-sensitive Yumai47. The proline content increased more significantly in Shi4185 than Yumai47; on the contrary, superoxide anion radical content increased less in Shi4185 than Yumai47. This data indicated that wheat salinity tolerance can be increased by controlling Na⁺ transport from the root to shoot, associated with higher osmotic adjustment capability and antioxidant activity. Although salinity increased aldehyde oxidase (AO) activity and abscisic acid (ABA) content in the leaves and roots of both cultivars following the addition of NaCl to the growth medium, AO and ABA increased more in the salt-sensitive cultivar Yumai47 than the more salt-tolerant cultivar Shi4185. Xanthine dehydrogenase (XDH) activity in the leaves of both cultivars increased with increasing concentrations of NaCl; however, leaf XDH activity increased more significantly in Yumai47 than Shi4185. Root XDH activity in Shi4185 decreased with increasing NaCl concentrations, whereas salinity induced an increased root XDH activity in Yumai47. The involvement of AO and XDH enzymatic activities and altered ABA content in the response mechanisms of wheat to salinity are discussed herein.

17

Application and prospect of graphene and its composites in wastewater treatment

64%

Liu J., Chen F., Yao Q., Sun Y., Huang W., Wang R., Yang B., Li W., Tian J.

Polish Journal of Environmental Studies

|

2020

|

tom 29

|

nr 6

18

The impacts of crustacean zooplankton on a natural ciliate community: a short-term incubation experiment

64%

Li J., Yang K., Chen F., Lu W., Fang T., Zhao X., Li H., Cui K.

Acta Protozoologica

|

2017

|

tom 56

|

nr 4

19

Ferrous bisglycinate increased iron transportation through DMT1 and PepT1 in pig intestinal epithelial cells compared with ferrous sulphate

64%

Liao Z.-C., Guan W.-T., Chen F., Hou D.-X., Wang C.-X., Lv Y.-T., Qiao H.-Z., Chen J., Han J.-H.

Journal of Animal and Feed Sciences

|

2014

|

tom 23

|

nr 2

To study the different mechanisms of absorption of diverse iron sources, pig intestinal epithelial cells (IPEC) were incubated with various concentrations of ferrous sulphate and ferrous bisglycinate for different times to determine the expression of divalent metal transporter (DMT1), peptide transporter (PepT1), ferritin, transferrin receptor (TfR), and neutral amino acid transporter (ASCT1 and ASCT2) using RT-PCR and Western blotting. In the ferrous bisglycinate groups, the expression of DMT1 protein was higher at 24 h and a concentration of 1 mmol · l–1 (P < 0.05) and expression of PepT1 protein was higher in all treatments (P < 0.05); expression of TfR and ferritin mRNA was higher at 36 h, 0.5 mmol · l–1 and 1 mmol · l–1; and 24 h, 1 mmol · l–1; 36 h, 0.5 mmol · l–1 (P < 0.05), respectively; ASCT1 and ASCT2 mRNA expression was higher at 36 h, 0.5 mmol · l–1 (P < 0.05). We conclude that the more effective absorption of ferrous bisglycinate is associated with higher expression of iron-related transporters in IPEC cells.

20

Analysis of miRNA-seq combined with gene expression profile reveals the complexity of salinity stress response in Oryza sativa

64%

Wang Q., Geng T., Zhu S., Li R., Tong Y., Wang S., Chen F., Tang L., He Y.

Acta Physiologiae Plantarum

|

2017

|

tom 39

|

nr 12

Ograniczanie wyników

Numerical investigation of temperature distribution of thermal discharge in a river-type reservoir

Comparative and evolutionary analysis of new variants of psi-gliadin genes from three A-genome diploid wheats

Transcriptome of wax apple (Syzygium samarangense) provides insights into nitric oxide-induced delays of postharvest cottony softening

Climatic signals in tree rings of Juniperus turkestanica in the Gulcha River Basin (Kyrgyzstan), reveals the recent wetting trend of high Asia

A study on the fundamental factors determining the efficacy of siRNAs with high C-G contents

Comparative analysis of natural cold acclimation and deacclimation of two Magnolia species with different winter hardiness

Spatio-temporal variability of microbial eukaryotic community composition in a large shallow subtropical lake, assessed by 18SrRNA gene sequences

Photosystem II activity in the leaves and assimilative branches of alhagi sparsifolia Shap. under brief elevated temperature

Nitrogen removal from piggery wastewater within a sequencing batch reactor using pseudomonas putida

Expiration date prediction of biocontrol agent prepared with Bacillus subtilis B579 using the accelerated aging method

Cr (VI) removal from aqueous solution using starch and sodium carboxymethyl cellulose-coated Fe and Fe /Ni nanoparticles

Cloning, characterization and overexpression of the complete open reading frame of the lateral shoot-inducing factor from Jatropha Curcas

The heterogeneity of cell subtypes from a primary culture of human amniotic fluid

Effects of low nitrogen and drought stresses on proline synthesis of Jatropha curcas seedling

Shelf life extension of shrimp (white) using modified atmosphere packaging

The response of Mo-hydroxylases and abscisic acid to salinity in wheat genotypes with differing tolerances

Application and prospect of graphene and its composites in wastewater treatment

The impacts of crustacean zooplankton on a natural ciliate community: a short-term incubation experiment

Ferrous bisglycinate increased iron transportation through DMT1 and PepT1 in pig intestinal epithelial cells compared with ferrous sulphate

Analysis of miRNA-seq combined with gene expression profile reveals the complexity of salinity stress response in Oryza sativa