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1

Nitric oxide delays chlorophyll degradation and enhances antioxidant activity in banana fruits after cold storage

100%

Wang Y., Luo Z., Du R.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 04

The effect of nitric oxide (NO) on chlorophyll degradation and antioxidant response in banana fruits after cold storage was investigated. Pre-climacteric green banana fruit treated with NO donor sodium nitroprusside of 0.05 mM was stored at 7 °C for 15 days and then ripened at 22 °C for 6 days. Chilling injury (CI) index, color, chlorophyll, total phenolics, activities of chlorophyllase, Mg-dechelatase, catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (POD), phenylalanine ammonia- lyase (PAL) and polyphenol oxidase (PPO) of the banana peel, together with firmness, titratable acidity (TA) and total soluble sugar (TSS) of the banana pulp were analyzed in this research. NO treatment improved chilling tolerance of banana fruits as shown by the reduced CI index. The retarded color change of the peel and higher firmness, TA and TSS content of the pulp showed that ripening process of NO-treated banana fruit was delayed by 2 days. The activities of chlorophyllase and Mg-dechelatase were significantly inhibited by NO, resulting in the retention of chlorophyll content. Further investigation showed that activities of CAT, APX and POD were enhanced in NO-treated banana fruits. Compared to control fruits, NO treatment significantly promoted PAL activity and inhibited PPO activity, and thus led to higher levels of total phenolics content. Results suggested that NO could enhance both enzymatic and non-enzymatic antioxidant system, and thus retarded chlorophyll degradation of banana fruits. Due to its effect on inhibition of chlorophyll degradation enzyme activities and promotion of the antioxidant defense system, NO is suggested as a potential approach to preserve chlorophyll content and maintain fruit quality of banana fruits after cold storage.

2

Isolation and characterisation of a Myb transcription factor DkPA1 related to proanthocyanidin biosynthesis in C-PCNA and non-PCNA persimmon (Diospyros kaki Thunb.) fruit

100%

Su F., Zhang Q., Luo Z.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 07

Proanthocyanidins (PAs) are secondary metabolites that contribute to important plant traits like fruit astringency. Persimmon (Diospyros kaki Thunb.) is unique for accumulating abundant PAs in its fruit flesh. Two non-astringent-type mutants, namely, Japanese pollination-constant and non-astringent (J-PCNA) and Chinese PCNA (C-PCNA), were unable to accumulate PA naturally on the tree before harvest. It was clear that the decreased expression profile of a Myb transcription factor (TF) DkMyb4 in persimmon fruits results in the PCNA trait in J-PCNA mutant. However, the mechanism leading to the PCNA trait in C-PCNA mutant remains unclear. In this study, with the purpose of figuring out whether or not there was a certain Myb TF resulting in PCNA trait in C-PCNA mutant, a gene homologous to DkMyb4 was isolated from C-PCNA cultivar ‘Luotian-tianshi’, named DkPA1. Bioinformatics analysis showed that DkPA1 shared high similarity to DkMyb4 in amino acid level, and that DkPA1 was phylogenetically closer to PA-related Myb TFs. Expression pattern of DkPA1 coincided with expression of PA pathway genes, also consistent with PA accumulation model in both C-PCNA and non-PCNA fruit during fruit development. Though being absent in J-PCNA, we found there were MYBCORE cis-motifs in promoter of PA pathway Leucoanthocyanidin reductase (DkLAR) gene isolated from C-PCNA and non-PCNA, which was reported as a key cis-element for PA biosynthesis and target of Myb TFs. These results suggested that DkLAR was regulated by DkPA1 in C-PCNA and non-PCNA, it played much more important role in C-PCNA and non-PCNA PA biosynthesis than that in J-PCNA.

3

Effect of UV-C treatment on modulating antioxidative system and proline metabolism of bamboo shoots subjected to chilling stress

100%

Zeng F., Jiang T., Wang Y., Luo Z.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 11

Bamboo shoots are highly nutritious but difficult to preserve even under low temperature since they are sensitive to chilling. In the present research, bamboo shoots were exposed to UV-C light (4.1 kJ/m2) prior to 56 days storage at 1 C to evaluate the potential of UV-C treatment on alleviating chilling injury (CI) of bamboo shoots under cold storage. CI incidence and physiological and biochemical characteristics associated with CI were determined during storage. The results showed that UV-C treatment apparently inhibited the development of CI and resulted lower EI and MDA content than that of the control. Further investigation demonstrated that UV-C treatment increased activities of CAT, APX, GR and DPPH scavenging activity of bamboo shoots, which greatly contribute to alleviating CI. The increases in both O2 - production rate and H2O2 content of UV-C-treated bamboo shoots were significantly delayed, PAL activity was increased which contributed to enhancing accumulation of TP, and endogenous proline synthesis was promoted, which resulted from the increased activity of P5CS and decreased PDH activity. The results above suggest that the improved antioxidative defense system and enhanced proline accumulation is the underlying mechanism that UV-C treatment enhanced chilling tolerance in bamboo shoots.

4

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Dentition and relationships of the Jurassic mammal Shuotherium

100%

Kielan-Jaworowska Z., Cifelli R. L., Luo Z.

Acta Palaeontologica Polonica

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2002

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tom 47

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nr 3

The Middle Jurassic mammal Shuotherium has lower molars that possess a trigonid and talonid, but are unique in having the talonid situated in front of the trigonid, rather than behind it, as in molars of usual tribosphenic pattern. Shuotherium dongi Chow and Rich, 1982 was based on a dentary bearing seven teeth, originally interpreted as three premolars and four molars. Based on comparison with other groups of early mammals, we reinterpret the premolar–molar boundary in the holotype of S. dongi, and propose a dental formula of four (or more) premolars and three molars. The ultimate lower premolar (previously identified as the first molar) has a completely developed trigonid and no talonid or pseudo−talonid. We hypothesize that the mesial cingulid on molars of Australosphenida is a highly plausible structural antecedent to the pseudo−talonid of Shuotherium. This and other shared, derived features support a relationship of Shuotherium and Australosphenida as sister−taxa. We hypothesize that the common ancestor of Shuotherium + Australosphenida had a global distribution no younger than early Middle Jurassic, and that the respective clades diverged prior to full separation of Gondwanan and Laurasian landmasses.

5

Effects of elevated CO2 and temperature on growth and morphology of fir (Abies faxoniana, Rehd. e t Wils.) and native herbs in a treeline ecotone: an experimental approach

88%

Hou Y., Luo Z., Jenerette G. D., Qiao Y., Wang K.

Polish Journal of Ecology

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2010

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tom 58

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nr 2

Determining how changes in atmospheric CO₂ concentrations and climate affects growth of species is helpful for understanding plant community species shift in response to future environmental changes. In the present study, fir (Abies faxoniana) and native herbs from treeline ecotone of east Qinghai-Tibetan Plateau (altitude: 3230–3300 m) were exposed to ambient CO₂ or ambient +350 μmol·mol⁻¹ CO₂ concentration in combination with ambient or ambient +2°C air temperature for two years in enclosedtop chambers. The results showed that elevated CO₂ , elevated temperature and the combination of elevated CO₂ and temperature increased biomass, height and diameter of fir compared to the control. Elevated CO₂ decreased biomass of Deyeuxia scabrescens, but increased for Fragaria orientalis and Cardamine tangutorum. Except for Fragaria orientalis, herbaceous biomass decreased by elevated temperature. The combination stimulated growth of Fragaria orientalis and Cardamine tangutorum, but suppressed for Deyeuxia scabrescens and Carex kansuensis. The results also demonstrated that elevated CO₂ and temperature increased the crown size and altered the morphology of fir, with benefits for resource capture, and did not affect growth of herbs. Larger root to shoot ratio of fir contributed the enhancement of biomass, while negatively influencing the growth of some herbs. This indicates morphological changes of trees may modify their growth responses and species around them to environmental changes. The different effects of elevated CO₂ and temperature on the growth of species in treeline ecotone suggest that climate change may alter community composition and structure.

6

Soaking in H2O2 regulates ABA biosynthesis and GA catabolism in germinating cotton seeds under salt stress

88%

Kong X., Luo Z., Zhang Y., Li W., Dong H.

Acta Physiologiae Plantarum

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2017

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tom 39

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nr 01

7

Exogenous sucrose treatment accelerates postharvest tomato fruit ripening through the influence on its metabolism and enhancing ethylene biosynthesis and signaling

76%

Li D., Mou W., Wang Y., Li L., Mao L., Ying T., Luo Z.

Acta Physiologiae Plantarum

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2016

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tom 38

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nr 09

8

Detachment-accelerated ripening and senescence of strawberry (Fragaria x ananassa Duch. cv. Akihime) fruit and the regulation role of multiple phytohormones

76%

Chen J., Mao L., Mi H., Zhao Y., Ying T., Luo Z.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 09

To study the detachment stress on the ripeness of strawberry fruit, physiological characteristics of strawberry fruit on and off plant during ripeness and senescence processes were investigated. The results indicated that the ripeness of strawberry fruit upon detachment was accelerated, in terms of firmness, soluble solid content and especially color development. The color of fruit off plant changed rapidly from white to full red in 1–2 days. The respiratory rate in fruit off plant was strengthened, higher than that on plant. Abscisic acid level and ethylene production in fruit off plant were also higher than those on plant and auxin degradation was exacerbated by detachment. Expression levels of FaMYB1, FabHLH3 and FaTTG1 were generally reduced with phenotypes of redder color and more anthocyanin accumulation in fruit off plant. Results also suggested that the detachment initially stimulated ethylene and abscisic acid production and auxin degradation, which modulated ripening-related gene expression and at last enhanced fruit pigmentation.

9

Interaction of abscisic acid and auxin on gene expression involved in banana ripening

76%

Lu W., Mao L., Chen J., Han X., Ren X., Ying T., Luo Z.

Acta Physiologiae Plantarum

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2018

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tom 40

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nr 03

10

Methyl jasmonate-induced accumulation of metabolites and transcriptional responses involved in triterpene biosynthesis in Siraitia grosvenorii fruit at different growing stages

76%

Zhang K., Luo Z., Guo Y., Mo C., Tu D., Ma X., Bai L.

Acta Societatis Botanicorum Poloniae

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2016

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tom 85

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nr 3

The cucurbitane-type triterpenoid glycosides, mogrosides, are the main active components of Siraitia grosvenorii fruit. Squalene and cucurbitadienol are among the intermediates of the biosynthetic pathway for the formation of cucurbitane-type triterpenoid backbones of mogrosides. It is recognized that the exogenous application of methyl jasmonate (MeJA) increases the accumulation of secondary metabolites in various plant species. Here, the effect of MeJA (50, 200, and 500 μM) on the accumulation of squalene and cucurbitadienol in the fruits of S. grosvenorii at 10, 20, and 30 days after flowering (DAF) was tested for the first time. Since mogroside II E is the main cucurbitane-type triterpenoid present at this time, its concentration was also determined. The results show that MeJA can indeed promote squalene and cucurbitadienol accumulation, the application of 500 μM MeJA at 30 DAF being optimal. The concentration of squalene and cucurbitadienol increased up to 0.43 and 4.71 μg/g dry weight (DW), respectively, both of which were 1.2-fold greater than that of the control. The content of mogroside II E increased by 15% over the untreated group. We subsequently analyzed the expression of key genes involved in the mogroside biosynthetic pathway, including the 3-hydroxy-3-methylglutaryl-coenzyme A reductase gene (SgHMGR), squalene synthetase gene (SgSQS), cucurbitadienol synthase gene (SgCS), and cytochrome P450 (SgCYP450) with quantitative real-time PCR. The results showed that transcriptional levels of these genes were upregulated following the treatment described above. Additionally, their responses in the presence of MeJA was related to the concentration and timing of MeJA treatment.

11

Reactive oxygen species mediated placental oxidative stress, mitochondrial content, and cell cycle progression through mitogen-activated protein kinases in intrauterine growth restricted pigs

64%

Luo Z., Luo W., Li S., Zhao S., Sho T., Xu X., Zhang J., Xu W., Xu J.

Reproductive Biology

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2018

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tom 18

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nr 4

12

ERK is involved in the differentiation induced by diallyl disulfide in the human gastric cancer cell line MGC803

64%

Ling H., Zhang L., Su Q., Song Y., Luo Z., Zhou X. T., Zeng X., He J., Tan H., Yuan J.

Cellular and Molecular Biology Letters

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2006

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tom 11

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nr 3

Diallyl disulfide (DADS) is a major constituent of garlic. Previously, we found that DADS both inhibited proliferation in human gastric cancer cells in vitro and in vivo, and induced G2/M arrest. In this study, we investigated whether this differentiation effect was induced by DADS in human gastric cancer MGC803 cells, and whether it was related to an alteration in ERK activity. The results showed that the growth of MGC803 cells was inhibited by DADS. Cells treated with DADS displayed a lower nucleocytoplasmic ratio and tended to form gland and intercellular conjunction structures. The ConA-mediated cell agglutination ratio and cells’ ALP specific activity decreased. In MGC803 cells, dye transfer was limited to a few cells neighbouring the dye-injected cell and to a depth of 1–2 layers beneath the scrape site. However, after treatment with DADS, the LY (Lucifer Yellow) was transferred to several cells immediately neighbouring the microinjected cell and to a depth of 2–4 cell layers from the scrape site. This indicated that DADS induced differentiation in MGC803 cells. Western blot analysis revealed that although DADS did not influence the quantity of ERK1/2 protein expressed, it did decrease its phosphorylation in a concentration-dependent manner, compared with the controls. At 30 mg·L−1, DADS inhibited the activation of ERK1/2 in 15–30 min. These results suggested that the DADS-induced differentiation of MGC803 cells involved an alteration of the ERK1/2 signaling pathway.

13

Downregulation of the lycopene ε-cyclase gene confers tolerance to salt and drought stress in Nicotiana tabacum

46%

Shi J., Liu P., Xia Y., Wei P., Li W., Zhang W., Chen X., Cao P., Xu Y., Jin L., Li F., Luo Z., Wei C., Zhang J., Xie X., Qu L., Yang J., Lin F., Wang R.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 10

Lycopene ε-cyclase (ε-LCY) functions at a branch point of the carotenoid biosynthesis pathway and modulates the ratio of lutein to the β-carotenoids. RNA interference (RNAi) and overexpression (OE) of Ntε-LCY were used to evaluate the physiological roles of ε-LCY in Nicotiana tobacum. In leaves, strong accumulation of β branch carotenoids and high expression of carotenoid biosynthesis genes resulted from suppression of Ntε-LCY expression. RNAi plants showed enhanced salt and drought tolerance, while overexpression of the Ntε-LCY gene weakened tolerance to salt and drought stress, as compared to control. Further analysis revealed that RNAi plants exhibited less water loss and had lower reactive oxygen species levels than did WT plants after both the salt and drought treatments. Further, higher levels of ABA accumulated in the RNAi lines than in the WT lines under stress conditions. These results suggest that reduced Ntε-LCY expression can improve drought and salinity tolerance in Nicotiana tabacum by enhancing their ROS scavenging ability.

Ograniczanie wyników

Nitric oxide delays chlorophyll degradation and enhances antioxidant activity in banana fruits after cold storage

Isolation and characterisation of a Myb transcription factor DkPA1 related to proanthocyanidin biosynthesis in C-PCNA and non-PCNA persimmon (Diospyros kaki Thunb.) fruit

Effect of UV-C treatment on modulating antioxidative system and proline metabolism of bamboo shoots subjected to chilling stress

Dentition and relationships of the Jurassic mammal Shuotherium

Effects of elevated CO2 and temperature on growth and morphology of fir (Abies faxoniana, Rehd. e t Wils.) and native herbs in a treeline ecotone: an experimental approach

Soaking in H2O2 regulates ABA biosynthesis and GA catabolism in germinating cotton seeds under salt stress

Exogenous sucrose treatment accelerates postharvest tomato fruit ripening through the influence on its metabolism and enhancing ethylene biosynthesis and signaling

Detachment-accelerated ripening and senescence of strawberry (Fragaria x ananassa Duch. cv. Akihime) fruit and the regulation role of multiple phytohormones

Interaction of abscisic acid and auxin on gene expression involved in banana ripening

Methyl jasmonate-induced accumulation of metabolites and transcriptional responses involved in triterpene biosynthesis in Siraitia grosvenorii fruit at different growing stages

Reactive oxygen species mediated placental oxidative stress, mitochondrial content, and cell cycle progression through mitogen-activated protein kinases in intrauterine growth restricted pigs

ERK is involved in the differentiation induced by diallyl disulfide in the human gastric cancer cell line MGC803

Downregulation of the lycopene ε-cyclase gene confers tolerance to salt and drought stress in Nicotiana tabacum