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1

Methylprednisolone improves the survival of new neurons following transient cerebral ischemia in rats

100%
Jing Y., Hou Y., Song Y., Yin J.
Acta Neurobiologiae Experimentalis
|
2012
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tom 72
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nr 3
Cerebral ischemia induced the proliferation and differentiation of neural stem cells in discrete regions of brain. However, only a small fraction of the neural stem/progenitor cells survives. In this report, the effects of methylprednisolone (MP) on proliferation, differentiation and survival of neural progenitor cells were explored through early MP administration after occlusion of the middle cerebral artery (MCAo). Transient cerebral ischemia was induced by middle cerebral artery occlusion in Sprague Dawley male rats. One hour, 1 day, 3 days, 14 days, and 28 days after MCAo, neurological examination was performed to evaluate the neurological deficit. MCAo rats were randomly divided into two groups, MP-group was injected MP (30 mg/kg, i.p.) at 3 h, 12 h, and 24 h after MCAo, and vehicle group was injected equal saline (i.p.). Animals were sacrificed at 3 days, 14 days, and 28 days after MCAo. MP was found to decrease apoptosis and TNF-a and IL-6 expression at 3 days after MCAo in the ipsilateral striatum. Moreover, MP significantly increased the migrated new neurons (BrdU+/ DCX+) and immature neurons (BrdU+/Tuj1+) at day 14 after MCAo in the ipsilateral striatum. Likewise, MP increased the number of mature neurons (BrdU+/MAP2+) at 28 days after MCAo. However, MP did not affect the progenitor cell proliferation (BrdU+ and Nestin+) at day 3 after MCAo in the subventricular zone (SVZ), but improved the neurological deficit at day 1 and day 3 after MCAo. These results indicate that early MP administration can improve the neurological deficit and enhance the survival of new neurons in the ipsilateral striatum by inhibiting apoptosis and downregulation of inflammatory response after transient cerebral ischemia in rats.
2

Effect of UV-B radiation on the growth and antioxidant enzymes of Antarctic sea ice microalgae Chlamydomonas sp.ICE-L

100%
Wang Q., Hou Y., Miao J., Li G.
Acta Physiologiae Plantarum
|
2009
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tom 31
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nr 6
The effect of ultraviolet-B (UV-B) radiation on Antarctic phytoplankton has become an attractive ecological issue as a result of annual springtime ozone depletion. The effects of UV-B radiation on the growth and antioxidant enzymes were investigated using Antarctic sea ice microalgae Chlamydomonas sp. ICE-L as the material in this study. The results demonstrated that UV-B radiation could notably inhibit the growth, especially at high UV-B radiation intensity (70 µW cm⁻²). Malondialdehyde and O₂˙⁻ content in ICE-L increased rapidly in early days (1–3 days) exposed to UV-B radiation enhancement, then decreased rapidly. In the stress of UV-B radiation enhancement, the superoxide dismutase, peroxidase and Catalase activities of 1–4 days in ICE-L were obviously higher than those in the control, and their activities became higher at high UV-B radiation intensity (70 µW cm⁻²). These enzymes activity of 7 days would kept stable at low UV-B radiation intensity (35 µW cm⁻²), but kept high level at high UV-B radiation intensity (70 µW cm⁻²). However, the ascorbate peroxidase activity in ICE-L kept stable under the stress of UV-B radiation enhancement. The above experimental results indicated that the antioxidant enzyme system played an important role in the adaptation of Antarctic ice microalgae under the UV-B radiation change of Antarctic ecosystems.
3

A novel 3D-QSA2R model assisted with a log-normalized method and Its application in molecular modification

88%
Li M., Zhang W., Hou Y., Sun R., Li Y.
Polish Journal of Environmental Studies
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2020
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tom 29
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nr 5
4

Effects of elevated CO2 and temperature on growth and morphology of fir (Abies faxoniana, Rehd. e t Wils.) and native herbs in a treeline ecotone: an experimental approach

88%
Hou Y., Luo Z., Jenerette G. D., Qiao Y., Wang K.
Polish Journal of Ecology
|
2010
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tom 58
|
nr 2
Determining how changes in atmospheric CO₂ concentrations and climate affects growth of species is helpful for understanding plant community species shift in response to future environmental changes. In the present study, fir (Abies faxoniana) and native herbs from treeline ecotone of east Qinghai-Tibetan Plateau (altitude: 3230–3300 m) were exposed to ambient CO₂ or ambient +350 μmol·mol⁻¹ CO₂ concentration in combination with ambient or ambient +2°C air temperature for two years in enclosedtop chambers. The results showed that elevated CO₂ , elevated temperature and the combination of elevated CO₂ and temperature increased biomass, height and diameter of fir compared to the control. Elevated CO₂ decreased biomass of Deyeuxia scabrescens, but increased for Fragaria orientalis and Cardamine tangutorum. Except for Fragaria orientalis, herbaceous biomass decreased by elevated temperature. The combination stimulated growth of Fragaria orientalis and Cardamine tangutorum, but suppressed for Deyeuxia scabrescens and Carex kansuensis. The results also demonstrated that elevated CO₂ and temperature increased the crown size and altered the morphology of fir, with benefits for resource capture, and did not affect growth of herbs. Larger root to shoot ratio of fir contributed the enhancement of biomass, while negatively influencing the growth of some herbs. This indicates morphological changes of trees may modify their growth responses and species around them to environmental changes. The different effects of elevated CO₂ and temperature on the growth of species in treeline ecotone suggest that climate change may alter community composition and structure.
5

Phytoextraction potential of Solanum nigrum L. and Beta vulgaris L. var. cicla L. in Cd-contaminated water

88%
Li Y., Hu X., Song X., Hou Y., Sun L.
Polish Journal of Environmental Studies
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2015
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tom 24
|
nr 4
The potential of two Cd-hyperaccumulators, Solarium nigrum L. (SN) and Beta vulgaris L. var. cicla L. (BV), as phytoremediation plants to remove Cadmium (Cd) from contaminated water was evaluated in hydroponics. The results showed that Cd exposure induced chlorosis and inhibited biomass growth in SN. However, these symptoms did not appear in BV. In BV, the Cd concentrations increased from 14.2 to 314.7 mg kg⁻¹ in the shoots and 241.1 to 4547.9 mg kg⁻¹ in the roots when the Cd concentration in the nutrient solution increased from 0.5 to 50 µМ. The corresponding increase in SN was from 7.4 to 100.6 mg kg⁻¹ in the shoots and 30.2 to 2010.7 mg kg⁻¹ in the roots. Generally, ВV showed a higher tolerance and accumulation of Cd, while SN was more efficient for Cd removal due to its higher biomass.
6

Numerical simulation of the ducted propeller and application to a semi-submerged vehicle

88%
Zou J., Tan G., Sun H., Xu J., Hou Y.
Polish Maritime Research
|
2020
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tom 27
|
nr 2
7

Analysis of the sulfur dioxide column concentration over Jing-Jin-Ji, China, based on satellite observations during the past decade

88%
Hou Y., Wang L., Zhou Y., Wang S., Wang F.
Polish Journal of Environmental Studies
|
2018
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tom 27
|
nr 4
Atmospheric sulfur dioxide (SO₂) is a major air pollutant and has the most negative effect on atmospheric chemistry. In this study, measurements of SO₂ column concentration obtained from an ozone monitoring instrument (OMI) are used to investigate the temporal and spatial dynamics of SO₂. Temporal and spatial distributions of SO₂ concentrations obtained from OMI over the Beijing-Tianjin-Hebei (Jing-Jin-Ji) region from 2007 to 2016 are presented, and annual changes and trends in the seasonal cycle are shown. The annual variations of SO₂ concentration are found to have decreased 47.6% from 2007 to 2016, and the SO₂ concentration shows a high correlation with SO₂ emissions. SO₂ shows significant cyclical seasonal characteristics over Jing-Jin-Ji, with maximum values in winter and minimum in summer; both temperature and precipitation are negatively correlated with SO₂ concentrations in seasonal variations. In addition, the spatial distribution is unbalanced, and Shijiazhuang-Xingtai-Handan is found to be in highly polluted areas, which may be related to energy consumption. The overall trends in the average annual value of SO₂ in the 13 sites appear to have decreased since 2007, and SO₂ concentrations in the Shijiazhuang-Xingtai-Handan region decreased by 55% during the past decade, so the Chinese government has made great efforts in pollution reduction, and has achieved remarkable results.
8

Predictions of select control organic pollutant removal in subsurface flow wetlands

76%
Xu D., Shi Y., Duan Y., Wang Y., You Y., Yang A., Hou Y.
Polish Journal of Environmental Studies
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2015
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tom 24
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nr 6
9

Combinatorial regulation of CLF and SDG8 during Arabidopsis shoot branching

76%
Bian S., Li J., Tian G., Cui Y., Hou Y., Qiu W.
Acta Physiologiae Plantarum
|
2016
|
tom 38
|
nr 07
10

Genetic diversity and RNA-seq transcriptome analysis of tricholoma matsutake from sichuan province, China

76%
Ding X., Wang P., Hou Y., Wang M., Hou W., Li Y.
Polish Journal of Environmental Studies
|
2016
|
tom 25
|
nr 6
The sequences of the internal transcribed spacer (ITS) regions of ectomycorrhizal fungi collected from Sichuan Province were analyzed using a PCR primer pair specific to T. matsutake. The amplified fragments were sequenced and compared with each other to build a phylogenetic tree. The mRNA deep sequencing approach was adopted to identify differentially expressed T. matsutake genes among the transcriptomes developed from a Xiaojin sample. A phylogenetic analysis of the aligned sequences was performed using maximum-likelihood (ML) and neighbor-joining (NJ) analyses. The results clearly showed that the KD (KM657344) and BT (KM657342) strains were more closely related to each other than to other strains. Moreover, T. matsutake from Sichuan differed from those specimens derived from Heilongjiang, Yunnam, and Guizhou provinces of China, Finland, and Japan. Furthermore, there was extremely high homology among these T. matsutake samples, despite some genetic variation. In addition, the genome of T. matsutake was sequenced using Illumina sequencing technology (RNA-seq). In all, a total of 24,549,990 reads were obtained that yielded 18,266,492 high-quality clean reads. The quality reads were excluded later. The BLAST analysis of the sequence reads against the NR database indicated that T. matsutake shared a high number of contigs with Laccaria bicolor. The results also indicated that catalytic activity, metabolic processes, metabolic pathways, and biosynthesis of secondary metabolites were the main functions identified by gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. Phylogenetic analysis showed that T. matsutake growing in Sichuan differed from samples growing in other regions. The differences in secondary metabolites between the Sichuan and Xiaojin samples may be due to differences in metabolic pathways. Thus the study provides a foundation for understanding T. matsutake biogeography and origins, and identifies DEGs in the Xiaojin sample to help elucidate the molecular mechanisms in secondary metabolite synthesis.
11

Health risks of heavy metals uptake by crops grown in a sewage irrigation area in China

64%
Wang Z., Zeng X., Geng M., Chen C., Cai J., Yu X., Hou Y., Zhang H.
Polish Journal of Environmental Studies
|
2015
|
tom 24
|
nr 3
Ecological risks of heavy metal toxicity such as Cd, Cu, Pb, Zn, Cr, As, and Hg through crops (wheat and rice) grown in the Tianjin sewage irrigation area in northern China were studied in this paper. Wheat and rice samples as well as related soil samples from 77 select blocks were collected. The second grade of standards for Tianjin soil environmental quality was used for soil risk assessment. Chinese National Food Safe Criterion was used for health risk assessment of wheat and rice grains. Daily intake rate and Target hazard quotient were used for the potential health risk assessment of local population through the intake of wheat and rice grown in the sewage-irrigated site. The results showed that continuous application of wastewater has led to accumulation of heavy metals in the soil, and Cd, Zn, and Hg were the main pollutants. Zn and Cd were more mobile than other metals. Pb in wheat and rice had an ecological risk to human health. As and Hg in some rice samples as well as Cd, Zn, and As in some wheat samples had potential risk. Target hazard quotient (THQ) of individual metal was below 1.0, meaning the relative absence of health risks associated with intake of a single heavy metal through intake of either wheat or rice. THQs of As for wheat and rice would sum up to above 1.0, indicating As may pose a risk to the local population by intake of wheat and rice.
12

Histopathological and cytopathological findings in minipigs infected with Streptococcus suis serotype 2

52%
Xie C., Hou Y., Zhao Y., Cai X., Li C., Li P., Li Y., Su X., Yue X., Wang S., Liu Y., Yang W., Yuan C., Cu L., Hua X., Yang Z.
Bulletin of the Veterinary Institute in Puławy
|
2014
|
tom 58
|
nr 2
Five pathogen-free miniature pigs (minipigs) were infected with the virulent strain SH08 of Streptococcus suis 2 (SS2) by intramuscular injection. The pigs died consecutively within 72 h after the challenge. An additional five non-infected pigs were euthanised and used as controls. Microstructural observations showed that degeneration, bleeding, congestion, cellular necrosis, and an increase in inflammatory cells were present in all organs and tissues except the brain. Ultrastructural observations revealed mitochondrial vacuolation and malformed or missing cristae, indicating that infection of minipigs with strain SH08 of SS2 can lead to extensive lesions in major internal organs and tissues. The findings also demonstrated that the minipig is a useful model for the study of SS2 infection.
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