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Fraxinus angustifolia Vahl is a species native in the north-western Balkans, where it has a divided range. Most of its range is located in flood-prone riparian sites in the Pannonian plain but the species is also found on humid sites along some rivers in the Submediterranean region. We analysed morphologic variation within and between five populations (14 trees/population) located in the Subpannonian and Submediterranean region of Slovenia. Thirty two characters were assessed on ca. 80 leaves from each tree which were divided depending on exposure to sunlight (sun and shade leaves), and 8 traits on each fruit (ca. 50 fruits/tree). A hierarchically designed experiment using analysis of variance confirmed the significant contribution of all the analysed hierarchical levels of variation to the total variation. The results show that the differences between the trees in a single population are the greatest factor of variability of leaves, followed by differences between populations and differences which are the result of variation in exposure to sunlight. Leaves in shade positions are typically larger than those in sunny positions, and they have broader and shorter-pointed leaflets; in this feature they are very similar to the leaves of F. excelsior. Stepwise discriminant analysis showed that the traits which refer to the leaflet number and length are the best differentiating traits between individual populations. The Submediterranean population Dragonja stands out with the smallest leaves and the population Lijak from the same region is the most similar to Dragonja for most morphological traits. In general, phenotypic differences between the Subpannonian and the Submediterranean populations of F. angustifolia are minor and indistinct. Based on the differences ascertained by the study, we can only confirm the presence of F. angustifolia subsp. oxycarpa (Bieb. ex Willd.) Franco et Rocha Alfonso in this part of its natural range.
The conversion of old black pine plantations into ecologically more stable broadleaf forests is an important goal in many Mediterranean countries. Six experimental plots split between more and less productive sites were established in the sub-Mediterranean climate zone of south-western Slovenia in an area of mostly pure black pine stands. In November 2012 they were planted with six native broadleaf species (Celtis australis, Quercus petraea, Fagus sylvatica, Prunus avium, Juglans regia and Acer pseudoplatanus). The test plantations were intended to become small initial nuclei from which the introduced broadleaf species could later spread naturally into the old and partly degraded pine plantations. Seedling condition was assessed one year after planting. Based on their high survival rates after the first growing season, all tested species except F. sylvatica showed promising potential for future conversion of old pine stands in the Slovenian Karst region. However, the results of one growing season only are insufficient to draw firm conclusions. Of all planted species P. avium had the highest survival rate (95%) and was least susceptible to poor field conditions. Without exception, all species showed lower survival rate on the less productive site. Unlike other species, P. avium and J. regia seedlings had considerable height increment, while F. sylvatica had the lowest. From the results we can assume that perhaps old pine stands in the area have already considerably improved soil conditions and thereby enabled the introduction of broadleaves.
The effects of developmental lead (Pb²⁺) exposure on the anxiolytic-like effect of diazepam (5.0 mg/kg IP) and 8-OH-DPAT (0.3 mg/kg IP) were studied. Wistar dams were exposed to 250 ppm lead acetate in drinking water during pregnancy. Control rats were derived from dams that consumed tap water, and had no exposure to Pb²⁺ afterwards. Male offspring were tested at the age of 12 weeks. We studied the anxiolytic-like effect of diazepam and 8-OH-DPAT in an elevated plus maze device and the Vogel conflict test. Diazepam in doses of 5.0 mg/kg IP significantly increased the percentage of time spent on open arms in control rats being without effect in Pb²⁺-exposed animals. 8-OH-DPAT 0.3 mg/kg IP increased the percentage of time spent on open arms in both experimental groups (control and Pb²⁺), but the anxiolytic-like effect was much more pronounced in Pb²⁺-intoxicated animals. The benzodiazepine anxiolytic diazepam produced a significant effect in the Vogel conflict test in control rats. A 5.0 mg/kg dose of those drugs caused a significant increase in the number of electric shocks rats received. In the ontogenetically Pb²⁺-exposed rats diazepam also augmented the number of shocks accepted, but this effect was much less pronounced than in control animals. Conversely, 8-OH-DPAT at doses of 0.3 mg/kg IP was without effect in both tested groups as far as the anticonflict effect is concerned. The results of the present report demonstrated that exposure to Pb²⁺ during pregnancy induced hypersensitivity to 5-HT1A agonist mediated anxiolytic-like effect but attenuated that of benzodiazepine (diazepam).
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