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1

Wirusy roślinne jako wektory do wyrażania obcych genów

100%
Palucha A.
Biotechnologia
|
2002
|
nr 1
2

Glutamatergiczne receptory matabotropowe [mGluR] : nowe oblicze glutaminianu - nowe perspektywy terapii

63%
Palucha A., Tokarski K.
Wszechświat
|
1999
|
tom 100
|
nr 10-12
228-232
3

Niekonwencjonalne strategie ochrony roslin przed infekcjami wirusowymi

51%
Hulanicka D., Palucha A., Zagorski W.
Postępy Biochemii
|
1994
|
tom 40
|
nr 2
91-96
4

Elements regulating Potato leafroll virus sgRNA1 translation are located within the coding sequences of the coat protein and read-through domain

51%
Loniewska-Lwowska A., Chelstowska S., Zagorski-Ostoja W., Palucha A.
Acta Biochimica Polonica
|
2009
|
tom 56
|
nr 4
619-625
 Translation of viral proteins from subgenomic RNAs (sgRNAs) is a common strategy among positive-stranded RNA viruses. Unlike host mRNA, sgRNA of Potato leafroll virus (PLRV) does not possess a cap at its 5' end nor a poly(A) tail at the 3' terminus, both of which are known to be crucial for translation of RNA in eukaryotic cells. Here, we demonstrate, that in wheat germ extract (WGE) truncation of the sgRNA1 5' UTR increases translation efficiency, as it has previously been observed in rabbit reticulocyte lysate (RRL), whereas removal of the 3' UTR does not affect translation. We also describe two regulatory elements located within the coding sequence of the coat protein (CP) gene and its read-through domain (RTD) and are responsible for regulation of in vitro translation of the PLRV sgRNA1. The first element is composed of the purine sequence AAAGGAAA located between the AUG codons of the CP and 17K genes. Deletion of this domain or its substitution by pyrimidines reduced by half the translation of both genes, whereas deletion of the RTD resulted in a 3.6-fold reduction in translation efficiency. This is the first report of translation regulatory elements of plant viruses located within a coding region.
5

Otrzymanie transgenicznego ziemniaka odpornego na infekcje wirusem lisciozwoju ziemniaka

51%
Palucha A., Chrzanowska M., Zagorski W., Hulanicka D.
Biotechnologia
|
1997
|
nr 4
38-47
6

Transformacja u ogorka Cucumis sativus L.

51%
Szwacka M., Burza W., Palucha A., Malepszy S.
Biotechnologia
|
1997
|
nr 4
20-26
7

The influence of antidepressant treatments on protein kineses activity in rat brain

51%
Branski P., Palucha A., Pilc A.
Acta Neurobiologiae Experimentalis
|
1997
|
tom 57
|
nr 5
8

Involvement of protein kinase a in the mechanism of antidepressant action

51%
Palucha A., Branski P., Kroczka B., Pilc A.
Acta Neurobiologiae Experimentalis
|
1999
|
tom 59
|
nr 3
9

The neuroprotective effect of MTEP, a potent and highly selective mGluRS antagonist, on excitotoxic neuronal death

51%
Domin H., Kajta M., Palucha A., Smialowska M.
Acta Neurobiologiae Experimentalis
|
2005
|
tom 65
|
nr 3
10

Wykrywanie β-laktamaz o rozszerzonym zakresie działania (ESBL) w izolatach klinicznych Klebsiella pneumoniae: test dwóch krążków i test ATB BLSE

51%
Gniadkowski M., Trzcinski K., Palucha A., Hryniewicz W.
Diagnostyka Laboratoryjna i Wiadomości PTDL
|
1996
|
tom 32
|
nr 4
11

Niekonwencjonalne strategie ochrony roslin przed infekcjami wirusowymi

51%
Hulanicka M. D., Zagorski-Ostoja W., Palucha A.
Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa
|
1997
|
tom 50
119-126
12

Characterization of metabotropic receptors for excitatory amino acids which stimulate cyclic AMP accumulation in rat brain

51%
Palucha A., Branski P., Legutko B., Pilc A.
Acta Neurobiologiae Experimentalis
|
1997
|
tom 57
|
nr 5
13

Virus-like particles of potato leafroll virus as potential carrier system for nucleic acids

51%
Suluja E., Strokowskaja L., Zagorski-Ostoja W., Palucha A.
Acta Biochimica Polonica
|
2005
|
tom 52
|
nr 3
Potato leafroll virus is a member of the polerovirus genus. The isometric virion is formed by a coat protein encapsidating single-stranded, positive-sense, mono-partite genomic RNA with covalently attached viral protein at the 5' end. The coat protein of the virus exists in two forms: i) a 23 kDa protein, the product of the coat protein gene, and ii) a 78 kDa protein, the product of the coat protein gene and an additional open reading frame expressed by read-through of the coat protein gene stop codon. The aim of this work was the expression of potato leafroll virus coat protein-based proteins that would be able to assemble into virus-like particles in insect cells. These modified particles were tested for their ability to encapsidate nucleic acids. Two types of N-terminally His-tagged coat protein constructs were used for the expression in insect cells: one, encoding a 23 kDa protein with the C-terminal amino-acid sequence corresponding to the wild type coat protein and the second with additional clathrin binding domain at the C-terminus. The expression of these two proteins by a recombinant baculovirus was characterized by Western immunoblotting with antibodies directed against potato leafroll virus. The protection or putative encapsidation of nucleic acids by these two coat protein derivatives was shown by DNase I and RNase A protection assays.
14

Sekwencja nukleotydowa wirusa lisciozwoju ziemniaka

45%
Palucha A., Kujawa A., Zagorski-Ostoja W., Hulanicka D., Haenni A. L.
Nauka Polska
|
1993
|
tom 41
|
nr 4
77-79
15

Regeneration and transforamtion of polish cultivars of potato

45%
Nadolska-Orczyk A., Milkowska L., Palucha A., Czembor P., Orczyk W.
Acta Societatis Botanicorum Poloniae
|
1995
|
tom 64
|
nr 4
335-340
16

Wirus M ziemniaka: klonowanie genomowych kopii izolatow o odmiennej patogennosci

45%
Walczak I., Palucha A., Juszczuk M., Muchalski T., Szybiak-Strozycka U.
Biotechnologia
|
1999
|
nr 3
169-177
17

Behaviorai effects of repeated treatment with amitriptyline and citalopram in the olfactory buibectomy model of depression in C57BL/6J mice

39%
Legutko B., Dudys D., Ciszek M., Wieronska J., Palucha A., Branski P., Pilc A.
Acta Neurobiologiae Experimentalis
|
2005
|
tom 65
|
nr 3
18

Potential anti-anxiety effects of gropu II glutamate metabotropic receptor agonists in animal models

39%
Pilc A., Klodzinska A., Chojnacka-Wojcik E., Palucha A., Branski P., Kroczka B.
Acta Neurobiologiae Experimentalis
|
1999
|
tom 59
|
nr 3
19

Nucleotide sequence of RNA of Polish isolate of potato leafroll luteovirus

39%
Palucha A., Sadowy E., Kujawa A., Juszczuk M., Zagorski W., Hulanicka D.
Acta Biochimica Polonica
|
1994
|
tom 41
|
nr 4
The nucleotide sequence of the genomic RNA of a Polish isolate of a potato leafroll virus was determined. Some variations between the determined sequences were observed. A comparison of the frequency of sequence variants in particular regions of the genome is presented. The nucleotide sequence reported will appear in the EMBL Data Library under the accession number X74789.
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