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Oznaczano zawartość azotynów w próbkach wody pitnej z wybranych miejscowości bornego Śląska oraz terenów przyległych, zmodyfikowaną metodą Griessa. Porównano ponadto stężenia azotynów w wodach pobieranych z przewodów wodociągowych eksploatowanych w sposób ciągły oraz okresowo wyłączanych z eksploatacji
Lead can modify pro/antioxidant status by influencing antioxidant enzymes. As the results of experimental researches are divergent, the purpose of this research was to evaluate the activity of enzymes that play a vital role in the defence against ROS in blood of people protractedly exposed to lead compounds. The study population included 172 healthy employees of zinc and lead steelworks. Workers exposed to lead (L) were divided into 2 groups: the first included workers with mean lead concentration (PbB) from 25-35 µl/dl (LL group), and the second group of high exposure (HL group) - with PbB over 35 µl/dl. The administration workers were the control group. There were no significant changes in activity of catalase and mitochondrial SOD in the study population. The activity of ZnCu-SOD significantly increased, both in plasma and erythrocytes, but first in plasma in the LL subgroup by about 42% (p=0.044), and then in erythrocytes in the HL subgroup by about 23% (p=0.012) when compared to the control group. Concentration of TBARS-MDA increased both in serum and erythrocytes. In people protractedly exposed to lead (mean 15 ± 10 years), there is observed an increased activity of SOD in blood, which seems to be an adoptive mechanism against the raised amount of production of reactive oxygen species (ROS) caused by lead.
U osób zawodowo narażonych na związki ołowiu i w grupie kontrolnej oznaczono Stężenie ołowiu i cynkoprotoporfiryny w krwi, stężenie kwasu deltaaminolewulinowego w moczu oraz aktywność transferazy-S-glutationowej w erytrocytach. U osób, u których we krwi wartość Stężenia ołowiu waha się od 250 do 450 µg/dm3 obserwuje się wzrost aktywności transferazy-S-glutationowej w erytrocytach średnio o 25%.
The influence of a balanced diet (21 g% protein, 34 g% fat, 45 g% carbohydrate) with an isocaloric addition of non-oxidised or oxidised rapeseed oil, with and without garlic, on the development of hypercholesterolaemia was examined in 18 adult male rabbits divided into 3 equal groups. The rabbits from group 1 were fed fresh rapeseed oil, group II - received oxidised rapeseed oil, and group III - was given oxidised rapeseed oil and garlic. The concentration of 7-ketocholesterol and malondialdehyde (MDA) in plasma and free fatty acids (FAME) in blood serum was determined. The experiment lasted 24 weeks. At the beginning, and every six weeks, the rabbits were weighed and blood was taken. After the experiment was completed, the aorta was dissected for histological examinations. It was found that oxidised rapeseed oil caused an increase in the concentration of 7-ketocholesterol and FAME at the end of the experiment. MDA concentration increased in the 6th week of the experiment but did not appreciably change at the end of it. The obtained results suggested that the diet caused the development of hypercholesterolaemic alterations in the aorta wall and increased temporarily the level of 7-ketocholesterol, FAME, and MDA. Diet rich in oxidised oil modified significantly homeostasis of lipids in plasma and blood serum. The administration of garlic in such a diet inhibited atherosclerotic changes in the aorta wall and this seemed to be related to the decreasing concentration of 7-ketocholesterol and MDA in plasma and FAME in blood serum.
For 24 weeks, rabbits were fed feed containing non-oxidised or oxidised rapeseed oil. At the beginning of the experiment and even six weeks the rabbits were weighed and blood was taken. After the experiment was completed, their liver was dissected for biochemical and histological examinations. The activity of alanine aminotransferase, aspartate aminotrasferase, glutamate dehydrogenase, sorbitol dehydrogenase, and aldolase in blood plasma and liver were determined. Enzymes of the protein and liver metabolic pathways were determined using kinetic and spectrophotometric methods. The content of fatty acids was determined by means of fatty acid methyl ester concentration measurement using gas chromatography. It was found that the applied diet with oxidised rapeseed oil caused the development of slight liver steatosis and disturbances in the activity of enzymes involved in the liver pathways, despite the fact that it was a balanced diet, and differed only in the ratio of saturated to unsaturated fatty acids. The obtained results indicate that more profound oil oxidation and its increased supply in diet may result in the development of liver steatosis.
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