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A relationship between jasmonates and ethylene in regulation of some physiological processes in plants under stress conditions is presented. Jasmonates are naturally occurring plant hormones showing various important biological activities in the regulation of plant growth development and in defense responses against a wide variety of abiotic and biotic agents. Jasmonates have been reported to control ethylene biosynthesis in intact plants and their organs. Mechanical wounding and other abiotic (osmotic stress, water deficit, dessication stress, heavy metals, touch, ozone) and biotic stresses (pathogen infection and insect invasion) are well known to be common factors inducing ethylene and jasmonates biosynthesis, and reactive oxygen species generation (ROS). Jasmonates have been well known to interact with ethylene in regulation of different processes; various kinds of interactions were documented: 1) synergistic interaction (i.e gene expression of proteinase inhibitors, osmotin, defensin), 2) ethylene suppresses processes induced by jasmonates (i.e. biosynthesis of nicotine, vegetative storage proteins and lectins), 3) jasmonates suppress processes induced by ethylene (i.e. ethylene-induced apical hook). Jasmonic acid carboxyl methyltransfe- rase (JMT) is a key enzyme for jasmonate-regulated plant responses. Activation of JMT expression leads to production of methyl jasmonate (JA-Me). JA-Me can act as an intracellular regulator, a diffusible intercellular signal transducer, or an airborne signal mediating intra- and interplant communications. Jasmonates represent an integral part of the signal transduction chain between stress signals) and stress responses(s), in most cases of the induction of gene expression and the accumulation of defense specific proteins and secondary metabolites.
This study aimed to know the key chemical compound influencing gummosis in petioles of intact growing culinary rhubarb (Rheum rhabarbarum L.) with special emphasis on its sugar composition. The application of methyl jasmonate (JA-Me, 0.5 and 1% in lanolin, w/w) in the middle of intact petiole of growing rhubarb substantially induced gummosis in the entire petioles, below and above the treatment, within several days. JA-Me at 0.5% in lanolin greatly stimulated ethylene production in intact petiole of growing rhubarb, on the 3rd day after JA-Me treatment, ethylene level being increased five times or more. However, an ethylene-releasing compound, ethephon (2-chloroethylphosphonic acid, 1 and 2% in lanolin, w/w) alone had no effect on gummosis. Analysis of gum polysaccharides by a gel permeation chromatography with a Tosho TSK-gel G5000PW gel permeation column revealed that almost all of rhubarb gum polysaccharides were eluted near the void in this gel chromatography system, suggesting that molecular mass of rhubarb gum polysaccharides are more than 500 kDa, while precise mass has not been decided in this study. Analysis of gum sugar composition after hydrolysis revealed that rhubarb gums is rich in galactose (ca. 30%), arabinose (ca. 20%), and galacturonic acid (15–20%), although other sugars also existed in small quantities. These results suggest that the key chemical compound of gummosis in petioles of rhubarb is jasmonates rather than ethylene, and gum polysaccharides consist of not only pectic arabinogalactans but also homogalacturonans.
Changes in cell wall polysaccharides in oat (Avena sativa L.) leaf segments during senescence promoted by methyl jasmonate (JA-Me) were studied. During the incubation with water at 25 ºC in the dark, the loss of chlorophyll of the segments excised from the primary leaves of 8-day-old green seedlings was found dramatically just after leaf excision, and leaf color completely turned to yellow after the 3- to 4-day incubation in the dark. Application of 10 μM JA-Me substantially promoted the loss of chlorophyll corresponding with the chloroplast degradation. Cell wall polysaccharides in oat leaf segments mainly consisted of hemicellulosic and cellulosic ones. During the process of leaf senescence, the amount of hemicellulosic I and II, and cellulosic polysaccharides decreased, but little in pectic polysaccharides. JA-Me significantly enhanced the decrease in cellulosic polysaccharides, but little in hemicellulosic ones. Arabinose, xylose and glucose were identified as main constituents of neutral sugars of hemicellulosic polysaccharides. The neutral sugar compositions of hemicellulosic polysaccharides changed little during leaf senescence both in the presence or absence of JA-Me. These facts suggest that JA-Me affects sugar metabolism relating to cellulosic polysaccharides during leaf senescence.
Sugar compositions of polysaccharide of gums hi peach shoots induced by methyl jasmonate (JA-Me), ethephon and their mixture were determined, resulting in successful identification of uronic acid(s) and neutral sugars: arabinose, galactose, xylose and mannose. This result suggests that the mechanisms of polysaccharides biosynthesis of gums induced by different hormones are quite similar or almost the same. JA-Me did not induce leaf abscission in cutting shoots but substantially accelerated with ethephon when it was simultaneously applied. No leaf abscission in intact growing peach shoots was found when JA-Me was applied in the middle of petiole but it was strongly stimulated by the simultaneous application of ethephon. JA-Me extremely stimulated anthocyanin accumulation in cutting peach shoots at natural light conditions. Ethephon simultaneously applied with JA-Me partially prevented the promotmg-effect of JA-Me on anthocyanin accumulation. The application of aminooxyacetic acid, an inhibitor of phenylalanine ammonia lyase, in natural light conditions, resulted in the inhibition of anthocyanin accumulation induced by JA-Me in cutting peach shoots. These results suggest that JA-Me and ethylene, not only independently but also cooperatively, play an important role in some physiological phenomena in peach shoots.
In the present work, 2,3,5-triiodobenzoic acid (TIBA) was applied to uncooled tu­lip bulbs, cultivars Apeldoorn and Gudoshnik, before flower bud formation, at the beginning of July and after flower bud formation, in October and November. Shoot growth and flowering of partially dry-cooled bulbs were substantially stimulated. These results strongly suggest that TIBA partially replaces the cold requirement of the tulip bulbs. In addition, the effect of TIBA is similar to gibberellins applied exogen- ously to the bulbs. Such a gibberellin application partially substitutes for cold treat­ment. Gibberellin application stimulates shoot growth and flowering of tulips. The mode of action of TIBA is discussed in relation to auxin action in tulips.
This manuscript reports that for tulip bulbs ( Tulipa gesneriana L. 'Apeldoorn'), simultaneous application of methyl jasmonate (JA-Me) with gibberellic acid (GA) increases gum formation in the bulbs, compared to JA-Me applied alone. After the dry scales of the bulbs were removed, the bulbs were treated with JA-Me and GA starting from the beginning of July 20 until November 30. Treated bulbs were stored in a laboratory room in natural light conditions. Gums produced by each treatment were weighted one month after treatment. JA-Me, at concentrations of 0.5 and 1.0% in lanolin, was applied alone, and also applied simultaneously with GA at concentrations of 0.25, 0.5 and 1.0% in lanolin. All the concentrations of GA applied simultaneously with JA-Me, substantially stimulated gum production in tulip bulbs. The production of gums decreased gradually from the beginning of October. The possible mode of action of GA to stimulate gum production in tulip bulbs is also discussed. The focus is on sugar metabolism and ethylene production.
We have found that auxin, indole-3-acetic acid (IAA) substantially induces the formation of the secondary abscission zone in stem and petiole explants and in decapitated stem and petiole after excision of blade in intact plants of Bryophyllum calycinum when IAA at a concentration of 0.1% as lanolin paste was applied in the middle of these organs. The secondary abscission zone was formed at a few mm above of the treatment with IAA, and senescence of the part above abscission zone was observed. IAA additionally applied on the top of explants or top of the dacapitated stem or the debladed petiole totally prevented the secondary abscission zone formation and senescence induced by IAA applied in the middle of these organs. Possible mechanisms of the formation of the secondary abscission zone are discussed in terms of the interaction of auxin and ethylene.
Effects of auxin polar transport inhibitors, 2,3,5-triiodobenzoic acid (TIBA), 1-N-naphthylphthalamic acid (NPA) and methyl 2-chloro-9-hydroxyfluorene-9-carboxylate (morphactin IT 3456), as a lanolin paste, on root formation in cuttings of some species of Crassulaceae, such as Bryophyllum daigremontianum, B. calycinum, Kalanchoe blossfeldiana and K. tubiflora, were studied. Cuttings of these plants were easily rooted in water without any treatment. TIBA and morphactin IT 3456 completely inhibited root formation in the cuttings of these plants but NPA did not when these inhibitors were applied around the stem below the leaves. When TIBA and morphactin were applied around the stem near the top, but leaves were present below the treatment, the root formation was observed in B. calycinum and K. blossfeldiana but in a smaller degree than in control cuttings. These results strongly suggest that endogenous auxin is required for root formation in cuttings of Crassulaceae plants. The differential mode of action of NPA is discussed together with its effect on auxin polar transport.
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