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1

Morphogenetic pathway in petiole derived callus of Amorphophallus albus in vitro

100%

Hu J., Li J.

Acta Physiologiae Plantarum

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2008

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tom 30

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nr 3

Two different morphogenetic pathways, adventitious bud and corm-like structure (CLS), were observed on organogenic calli derived from the petioles of Amorphophallus albus in vitro. The organogenic calli was established via culture of petiole segments on Murashige and Skoog (MS) medium supplemented with 1.0 mg l⁻¹ a-naphthaleneacetic acid (NAA) and 1.0 mg l⁻¹ 6-benzyladenine (BA) and subculture of the petiole-derived calli on MS medium with 0.5 mg l⁻¹ NAA and 0.5 mg l⁻¹ BA. These organogenic calli were used to induce morphogenesis via culture on MS medium with various concentrations of NAA and BA. BA alone favoured adventitious bud differentiation (57.0 ± 8.3% at maximum) from the organogenic calli but inhibited CLS formation. In the presence of NAA and BA, both adventitious bud and CLS were observed in a same culture system. The maximum CLS formation (71.2 ± 9.3%) were found on MS medium with 0.5 mg l⁻¹ NAA and 2.0 mg l⁻¹ BA, associated with 26.7 ± 8.6% adventitious bud differentiation. A small part of the adventitious buds developed into normal shoots which needed rooting culture phase to form complete plants. About 80% survival rate was obtained with these plants after transplantation to soil. More than 90% of the CLSs produced complete plants with shoots and root systems, regardless of the rooting media tested. Transplantation of the CLS-derived plants to soil gave 100% survival rate. Histological observations revealed both the two morphogenetic events originated from the meristematic cells located in superficial layers of callus tissue.

2

Loureirin B inhibits the proliferation of hepatic stellate cells and the Wnt/β-catenin signaling pathway by regulating miR-148-3p

81%

Hu J., Zhang R., Tang M., Li Y.

Cellular and Molecular Biology Letters

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2018

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tom 23

Background: We investigated the activity of loureirin B against liver fibrosis and the underlying molecular mechanisms. Methods: Hepatic stellate cells (HSCs) from Sprague-Dawley rats were treated with different concentrations of loureirin B. We used the MTT assay to determine HSC proliferation, flow cytometry to analyze apoptosis, and western blot to determine the expressions of Bax, Bcl-2, Wnt1 and β-catenin. Real-time PCR was used to determine the expressions of Wnt1 and miR-148-3p. Results: The MTT assay showed that loureirin B treatment significantly inhibited the proliferation of HSCs in time- and dose-dependent manners. Loureirin B significantly promoted the apoptosis of HSCs, increased the expression of Bax and decreased the Bcl-2 level. Western blot analysis showed that the expressions of Wnt1 and β-catenin were obviously lower in the loureirin B treatment group than in the control group. We also found that loureirin B could decrease the Wnt1 mRNA level and increase miR-148-3p expression. Knockdown of miR-148-3p using inhibitor could reverse the effects of loureirin B on the proliferation and apoptosis of HSCs and the expressions of Bax, Bcl-2, Wnt1 and β-catenin. Conclusion: Our results suggest that loureirin B inhibited the proliferation and promoted the apoptosis of HSCs, and suppressed the Wnt/β-catenin signaling pathway via regulation of miR-148-3p.

3

Marine search and rescue of UAV in long-distance security modeling simulation

81%

Zheng L., Hu J., Xu S.

Polish Maritime Research

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2017

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tom 24

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nr Special Issue S3

Long-distance safety of Marine search and rescue using drones can improve the searching speed. The current method is based on the long distance security classification of UAV.The degree of accuracy is low. A long-distance security modeling approach based on ArduinoMiniPro’s Marine search-and-rescue applying UAV is proposed. The method puts the fault tree analysis and relevant calculation for risk identification into use. The main factors affecting the safety of unmanned aerial vehicle (UAV) are long-distance searching and rescuing. The experimental results show that the proposed method can effectively build modeling for the long-distance safety of the Marine search and rescue UAV

4

Two new species of the genus Nazeris Fauvel of Fujian Province, China (Coleoptera: Staphylinidae: Paederinae)

81%

Hu J., Li L., Zhao M.

Annales Zoologici

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2010

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tom 60

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nr 3

Two new species of the genus Nazeris Fauvel collected from Meihuashan Nature Reserve, Fujian Province, are described under the names of N. fujianensis sp. nov. and N. xuiwangi sp. nov. The male sexual characters are described and illustrated.

5

The identification and characterization of a testis-specific cDNA during spermatogenesis

81%

Chen Y., Hu J., Song P., Gong W.

Cellular and Molecular Biology Letters

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2006

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tom 11

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nr 1

Using bioinformatics and experimental validation, we obtained a cDNA (named srsf) which was exclusively expressed in the mouse testes. RT-PCR analysis showed that srsf mRNA was not expressed in the gonad during the sex determination period or during embryogenesis. In developing mouse tests, srsf expression was first detected on post-natal day 10, reached its highest level on day 23, and then reduced to and remained at a moderate level throughout adulthood. In situ hybridization analysis demonstrated that srsf mRNA was expressed in pachytene spermatocytes and round spermatids in the testes. The predicted protein contains one RNA-binding domain (RBD) and a serine-arginine rich domain (RS), which are characterized by some splicing factors of SR family members. These findings indicate that srsf may play a role during spermatogenesis.

6

Comparative effects of aquaculture and water level fluctuations on macroinvertebrate communities in Three Gorges Reservoir, China

72%

Chi S., Zheng Z., Zhang Z., Hu J., Zheng J., Zhao X., Hu J., Dong F., Peng J.

Polish Journal of Ecology

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2017

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tom 65

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nr 4

In this study, the comparative effects of the two disturbances (aquaculture and water level fluctuations) on macroinvertebrate communities were explored in two waterbodies connected with the reservoir system of Three Gorges Reservoir (TGR) in China. One water body called Gaoyang Bay which suffered organic pollution from intensive aquaculture. Another named Hanfeng Lake, where the effects of water-level fluctuations are obvious. The results showed that aquaculture could significantly affect the chemical forms of nutrients, decrease the α-diversity and increase the β-diversity of macroinvertebrates although the communities in the treatment area in Gaoyang Bay were not fundamentally changed comparing to the control area in the same bay. The densities of macroinvertebrates in the treatment area were significantly lower than that of the control area. The composition of functional feeding groups in the treatment area was close to that in Hanfeng Lake, but obviously different from that in the control area in Gaoyang Bay and the collectors and predators dominated the communities in this control area with the highest percentages. Although water-level fluctuations had negative effects on the communities by decreasing the α-diversity and increasing the β-diversity, which were confirmed by the values of Simpson index, Shannon-Wiener index and Harrison's index in the control area of Gaoyang bay and Hanfeng Lake, the adverse effects were relative low compared to aquaculture. In the bays of TGR, the small-scale disturbance (aquaculture activities) had more significant negative effects compared to the big-scale disturbance (water-level fluctuations related to dam operation of TGR).

7

A new anti-counterfeiting method: fluorescent labeling by safranine T in tobacco seed

71%

Guan Y., Hu J., Li Y., Ma W., Zheng Y.

Acta Physiologiae Plantarum

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2011

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tom 33

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nr 4

Counterfeit seeds cause a large loss in seed industry and crop production. Present study was undertaken to find a new anti-counterfeiting technology by labeling seeds with a fluorescent compound, safranine T (ST). Response surface analysis (RSA) experiment with two factors (ST concentration and seed soaking time) of 14 combinations was performed to investigate the ST effects on seed vigor and fluorescence showing in seedlings of two tobacco varieties, MS Yunyan 85 and Honghua Dajinyuan. After soaking in ST solutions, tobacco seeds were germinated for 16 days, then the germination index (GI), vigor index (VI), length of roots and shoots were determined. The optimum combinations of ST concentration and soaking time on GI, VI and length of shoots and roots were 0.57–0.69 mg/ml and 6.77–9.34 h for MS Yunyan 85 and 0.51–0.80 mg/ml and 5.81–7.39 h for Honghua Dajinyuan, respectively. Meanwhile, tobacco seedlings, treated with ST solutions from 0.3 to 1.5 mg/ml showed obviously fluorescence under illumination of green light. It suggests that soaking tobacco seeds in safranine T solution of suitable concentration can be used as a labeling technique for anti-counterfeiting in tobacco seeds.

8

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Redox-mediated regulation of wheat seed dormancy revealed through modification-specific proteomics

71%

Bykova N., Hoehn B., Rampitsch C., Hu J., Fan T.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

9

A low-carbon generation expansion planning model considering carbon trading and green certificate transaction mechanisms

71%

Huang Y., Hu J., Yang Y., Yang L., Liu S.

Polish Journal of Environmental Studies

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2020

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tom 29

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nr 2

10

Salt stress induced sex-related spatial heterogeneity of gas exchange rates over the leaf surface in Populus cathayana Rehd.

71%

Xu X., Li Y., Wang B., Hu J., Liao Y.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 01

The sex-related spatial heterogeneity of gas exchange rates over the leaf surface under salt stress was investigated in the dioecious species, Populus cathayana Rehd. Cuttings were subjected to two salt regimes: 0 and 75 mM NaCl added to the Hoagland solution, the control and the treatment group, respectively. Measurements of gas exchange parameters were taken from over 40 sites on the surfaces of representative ‘non-stressed’ and ‘salttreated’ leaves which had the same insertion point for two sexual cuttings. Compared to the control group, the treatment group showed a significant decrease in the mean values of the following: water use efficiency (WUE), Chlorophyll a (Chl a) concentration, chlorophyll b (Chl b) concentration, concentration of carotenoids (Caro), total chlorophyll concentration (TC) in two sexes, and net photosynthesis rate (Pn), stomatal conductance (gs), and stomatal length/width ratio (SR) in females. Also, in the treatment group, females exhibited lower WUE, Pn, gs, E, Chl a, Chl b, TC, and SR than males. Comparison of contour maps showed that the net photosynthesis rate decreased gradually from apical to basal zones over the leaf surface occurred in the two sexes under natural conditions, but under salt stress, the opposite trend was found in females only. The results suggest that the heterogeneity pattern of the gas exchange parameters in response to salt stress between the two sexes is quite different due to different strategies employed by males and females to maintain the photosynthesis rate under salt stress. This heterogeneity phenomenon under salt stress may mainly be attributed to the chlorophyll pigments in males and the stomatal apertures in females.

11

Motion characteristics after ricochet: an experimental investigation

71%

Chen G., Hu J., Liu A., Feng J., Hu Q.

Polish Maritime Research

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2020

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tom 27

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nr 2

12

miR-3188 Regulates proliferation and apoptosis of granulosa cells by targeting KCNA5 in the polycystic ovary syndrome

71%

Zhou S., Xia L., Chen Y., Guo W., Hu J.

Acta Biochimica Polonica

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2021

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tom 68

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nr 1

13

MITF and PU.1 inhibit adipogenesis of ovine primary preadipocytes by restraining C/EBPβ

71%

Ruan C., Li X., Hu J., Zhang J., Zhao X.

Cellular and Molecular Biology Letters

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2017

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tom 22

14

Regional hypoxia correlates with the uptake of a radiolabeled targeted marker of angiogenesis in rat model of myocardial hypertrophy and ischemic injury

71%

Dobrucki L. W., Meoli D. F., Hu J., Sadeghi M. M., Sinusas A. J.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

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nr 4

15

Food habits and space-use of red pandas Ailurus fulgens in the Fengtongzhai Nature Reserve, China: food effects and behavioural responses

71%

Zhang Z., Hu J,, Yang J., Li M., Wei F.

Acta Theriologica

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2009

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tom 54

|

nr 3

225-234

The red panda Ailurus fulgens Cuvier, 1825 is endemic to the Himalayan-Hengduan Mountains. Many of its ecological traits are still poorly understood although important from a scientific standpoint as a highly specialized bamboo feeder. To understand its food habits and space-use, we carried out a field study from April 2002 to May 2003 in Fengtongzhai Nature Reserve, China. The results showed that red pandas almost exclusively fed on Bashania faberi, but not on Yushania brevipaniculata in the study area. Besides differences in nutritional quality, accessibility to food items is perhaps another factor affecting their utilization of bamboo species. B. faberi leaves are the highest in crude protein, being the primary year-round food source; new shoots and fruits of Sorbus, and Rubus are nutritious and digestible, seasonally occurred in their diet. Red pandas seemed to select most nutritious food items to feed on. The home range of red pandas averaged 2.2 km2 across individuals (2.6 km2 for the males and 1.7 km2 for the females), and overlapped extensively (ranging 18.9–78.1%). No significant difference was found in monthly percentage of total home range used among seasons and sexes. The daily movement distance was 455 m (447 m for the females and 463 m for the males), significantly affected by sexes and months. Our findings indicated that food resource significantly influenced behaviour of red pandas in foraging and space use, and their behavioural responses are adaptive being helpful to energy intake and maintenance.

16

Stability control of propeller autonomous underwater vehicle based on combined sections method

61%

Qi D., Feng J., Liu A., Hu J., Xu H., Li Y., Ashraf M. A.

Polish Maritime Research

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2015

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tom 22

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nr Special Issue S1

Learning from the motion principle of quadrotor, a symmetric propeller AUV, which has small size and low velocity is designed. Compared with the AUV equipped with rudders, it has better maneuverability and manipulation at low velocity. According to the Newton-Euler method, the 6 DOF kinematic model and dynamic model of the propeller AUV are established. A stability controller that consists of 3 different PID controllers is designed. It makes the depth and attitude angle as trigger conditions, and the relevant controller is chosen in different moving process. The simulation experiments simulate ideal motion state and disturbed motion state, and experiments results show that the stability controller based on combined sections method can make the best of mature technology of PID, and meet the control requirements in different stages. It has a higher respond speed and accuracy, improving the stability of the propeller AUV under the disturbance of complex ocean currents

17

Factors regulating the compositions and distributions of dissolved organic matter in the estuaries of Jiaozhou Bay in North China

61%

Hu J., Zou L., Wang J., Ren Q., Xia B., Yu G.

Oceanologia

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2020

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tom 62

|

nr 1

18

Water-exit process modeling and added-mass calculation of the submarine-launched missile

61%

Yang J., Feng J., Li Y., Liu A., Hu J., Ma Z.

Polish Maritime Research

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2017

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tom 24

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nr Special Issue S3

In the process that the submarine-launched missile exits the water, there is the complex fluid solid coupling phenomenon. Therefore, it is difficult to establish the accurate water-exit dynamic model. In the paper, according to the characteristics of the water-exit motion, based on the traditional method of added mass, considering the added mass changing rate, the water-exit dynamic model is established. And with help of the CFX fluid simulation software, a new calculation method of the added mass that is suit for submarine-launched missile is proposed, which can effectively solve the problem of fluid solid coupling in modeling process. Then by the new calculation method, the change law of the added mass in water-exit process of the missile is obtained. In simulated analysis, for the water-exit process of the missile, by comparing the results of the numerical simulation and the calculation of theoretical model, the effectiveness of the new added mass calculation method and the accuracy of the water-exit dynamic model that considers the added mass changing rate are verified

19

Stability analysis of dangerous rockmass considering rainfall and seismic activity with a case study in China’s Three Gorges area

61%

Hu J., Liu H., Li L., Zhou S., Zhang Q., Sun S.

Polish Journal of Environmental Studies

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2019

|

tom 28

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nr 2

Rainfall and seismic activity have significant influence on the stability of dangerous rockmass. In this paper, the dangerous rockmasses are classified into three instability types according to the failure modes: sliding, falling, and toppling. The discrete element method (COMSOL) was carried out to investigate the stability of three different rockmass types. In the numerical simulation, three load combinations were applied to the numerical model: 1) rockmass weight, 2) rockmass weight + fissure water pressure, and 3) rockmass weight + fissure water pressure + earthquake force. The results show that rainfall and seismic activity play important roles in rockmass stability and the displacement and principal stress of the rockmass are influenced obviously. Wangxia dangerous rockmass in the Great Three Gorges area was selected as a case study, and its stability was analyzed by geological investigation, numerical simulation, and static calculation methods. The results show that there is a high risk that Wangxia dangerous rockmass would lose stability when rainfall season comes and seismic activity happens. A blast was conducted to remove this dangerous rockmass and an SNS net design was proposed to intercept distributed blocks based on rockfall trajectory simulation. The parameters needed in the rockfall simulation program were obtained by laboratory and field tests. Final application proved that the designed treatment is efficient and no damage was caused by the distributed blocks. The whole research may provide a useful reference to similar engineering projects in the future.

20

Risk assessment of rockfall hazards in a tunnel portal section based on normal cloud model

61%

Wang X.-T., Li S.-C., Ma X.-Y., Xue Y.-G., Hu J., Li Z.-Q.

Polish Journal of Environmental Studies

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2017

|

tom 26

|

nr 5

In mountainous regions, rockfall is a typical geological disaster which might bring immense casualties and economic losses, but also endanger the safety of civil engineering construction. Many tunnels are being built in the southwest of China, thus a comprehensive assessment for rockfall risk is needed. For this purpose, in this paper, based on normal cloud model theory, we created a multi-index evaluation model for the rockfall risk assessment. Then, according to previous research and specific geological conditions, potential tunnel dangers are classified into four ranks, and some geological factors are considered as the principal factors. In order to fully express the opinions of experts, the qualitative indices were quantified by continuous value scale. Moreover, the value of each index is determined by expert scoring. In view of different evaluation units, we used the normalization method to make geological indices dimensionless. And three numerical characteristics (Ex, En, and He) were calculated by the cloud generator algorithm with MATLAB. In this study, we assigned the weight of indices by simple dependent function to avoid the influence of subjective. Finally, by means of a normal cloud generator, we determined the integrated certainty grades. To ensure the accuracy of the normal cloud model method, it was tested in rockfall cases in Jiefangcun tunnel. And the results obtained by the cloud model method are in good agreement with the practical situation. Moreover, the results are better than those of the AHP-FUZZY and artificial neural networks methods after comparison. The cloud model-based method realizes a multi-criteria assessment of the rockfall risk in tunnel portal section and provides a practical guide on safe tunnel construction for similar projects.

Ograniczanie wyników

Morphogenetic pathway in petiole derived callus of Amorphophallus albus in vitro

Loureirin B inhibits the proliferation of hepatic stellate cells and the Wnt/β-catenin signaling pathway by regulating miR-148-3p

Marine search and rescue of UAV in long-distance security modeling simulation

Two new species of the genus Nazeris Fauvel of Fujian Province, China (Coleoptera: Staphylinidae: Paederinae)

The identification and characterization of a testis-specific cDNA during spermatogenesis

Comparative effects of aquaculture and water level fluctuations on macroinvertebrate communities in Three Gorges Reservoir, China

A new anti-counterfeiting method: fluorescent labeling by safranine T in tobacco seed

Redox-mediated regulation of wheat seed dormancy revealed through modification-specific proteomics

A low-carbon generation expansion planning model considering carbon trading and green certificate transaction mechanisms

Salt stress induced sex-related spatial heterogeneity of gas exchange rates over the leaf surface in Populus cathayana Rehd.

Motion characteristics after ricochet: an experimental investigation

miR-3188 Regulates proliferation and apoptosis of granulosa cells by targeting KCNA5 in the polycystic ovary syndrome

MITF and PU.1 inhibit adipogenesis of ovine primary preadipocytes by restraining C/EBPβ

Regional hypoxia correlates with the uptake of a radiolabeled targeted marker of angiogenesis in rat model of myocardial hypertrophy and ischemic injury

Food habits and space-use of red pandas Ailurus fulgens in the Fengtongzhai Nature Reserve, China: food effects and behavioural responses

Stability control of propeller autonomous underwater vehicle based on combined sections method

Factors regulating the compositions and distributions of dissolved organic matter in the estuaries of Jiaozhou Bay in North China

Water-exit process modeling and added-mass calculation of the submarine-launched missile

Stability analysis of dangerous rockmass considering rainfall and seismic activity with a case study in China’s Three Gorges area

Risk assessment of rockfall hazards in a tunnel portal section based on normal cloud model