Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 16

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Procaine injections into the PPN suppress behaviour evoked by stimulation of the VTA

100%
Jerzemowska G.
Acta Neurobiologiae Experimentalis
|
2007
|
tom 67
|
nr 3
2

c-fos expression in rats hyper- and hyporesponsive to novelty in basal conditions and after chronic electric stimulation of the ventral tegmental area (VTA)

51%
Krzyzak A., Ledochowski P., Jerzemowska G., Trojniar W.
Acta Neurobiologiae Experimentalis
|
2005
|
tom 65
|
nr 3
3

Morphological changes in the midbrain dopaminergic cells in response to new environmental and after electrical stimulation of the ventral tegmental area in rats

51%
Jerzemowska G., Plucinska K., Kulikowski M., Wrona D.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
The aim of the study was to test whether increased activity of the telencephalic dopaminergic systems found in rats differing in locomotor activity to novelty (high responders; HR or low responders; LR) is associated with differences in the morphology of cells containing the enzyme tyrosine hydroxylase (TH) in the main group of the brain dopaminergic neurons. The morphology of cells were analyzed in conditions of exposure to a new environment and after chronic electrical stimulation of the ventral tegmental area (VTA). Two groups of male Wistar rats were used: subjected to a new environment (moving from vivarium to the experimental room) and subjected to a 14-day unilateral electrical stimulation of the VTA, which produces behavioral signs of psychomotor activation. After termination of the stimulation procedure, all rats were subjected to the immunohistochemical and immunofluorescent staining of neurons expressing TH (TH+ cells). We analyzed the whole cell: measure area (μm2 ), perimeter, major and minor axis length (μm) and circularity factor (μm2 / μm2 ). We found that chronic electrical stimulation of the VTA causes significant changes in the morphology of TH+ cells as manifested by changes in their normal shape and distribution of pigment (immunohistochemical labeling: in the rats after stimulation of the VTA in comparison to the naive gropus grayscale value >70% vs >50%), as well as increasing the size of cells in both HR and LR rats (immunofluorescence labeling: area p<0.01, perimeter p<0.001, major axis length p<0.05 and circularity factor p<0.05 in the HR and LR rats after stimulation of the VTA in comparison to the HR and LR naive rats). The results obtained suggest that individual behavioral and neurochemical differences which are correlated with increased susceptibility to stress and propensity to develop drug addictions, which characterize HR animals, may be connected with alterations in the morphology and activity of the dopaminergic systems.
4

Amphetamine injection into contralateral nucleus accumbens shell alters feeding evoked by stimulation of the mesolimbic system

51%
Jerzemowska G., Plucinska K., Ptaszek K.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Dopamine projections from the ventral tegmental area (VTA) – a somatodendritic region of mesolimbic system to the nucleus accumbens (particularly its shell region, AcbSh) – which constitutes its main terminal structure may play a central role in modulating affective states. In the present work, we used model of the VTA electrical stimulation-induced feeding reaction to research the role of AcbSh dopamine transmission in motivated behaviors. METHODS: In 5 rats (n=5) latency to eat was measured as a function of stimulation frequency before and after contralateral intraAcbSh injection of dopamine agonist D-amphetamine (dose 5.0 µg dissolved in 0.5 µl of water). This experimental method allowed us to distinguish between motivational vs. motor aspects of tested reaction. RESULTS: Inactivation of theAcbSh caused of itsintra injection of dopamine agonist D-amphetamine on the contralateral side (in relation to the hemisphere with VTA stimulation) affects motivational processes assessed by changes in frequency threshold for stimulation-induced feeding response. We observed increase, by more than 10% (about 13.37%), in the reaction threshold as compared with water control. Increase of the frequency threshold was accompanied by a parallel rightward shift of the function relating latency to feed to stimulation frequency, but statistical analysis of latency at the specific frequencies showed no significance (from 17.71 Hz to 81.38 Hz, P>0.05). Distilled water injected into the contralateral AcbSh (volume of the 0.5 µl), as a control group (n=5), did not cause any effect in comparison with the preinjection baseline. CONCLUSION: We conclude that the contralateral inactivation at the level of AcbSh does not impair of the behavior induced by activity of the dopaminergic cells at the level of VTA. Research was financed by the Polish National Science Centre (NCN); decision no: DEC-2013/09/D/NZ4/02499.
5

Changes in the number and morphology of the dopaminergic neurons after electrical stimulation of the ventral tegmental area

51%
Trojniar W., Jerzemowska G., Tokarski J.
Acta Neurobiologiae Experimentalis
|
2003
|
tom 63
|
nr 5
6

Interhemispheric relations in the motor function of the subthalamic region

51%
Ledochowski P., Grembecka B., Jerzemowska G., Trojniar W.
Acta Neurobiologiae Experimentalis
|
2007
|
tom 67
|
nr 3
7

Brain c-fos expression after unilateral electrolytic lesion and contralateral electrical stimulation of the ventral tegniental area in rats

51%
Prabucka I., Jerzemowska G., Cecot T., Trojniar W.
Acta Neurobiologiae Experimentalis
|
2005
|
tom 65
|
nr 3
8

Midbrain dopaminergic cells after unilateral lesion and contralateral stimulation of the ventral tegmental area (VTA)

51%
Cecot T., Jerzemowska G., Trojniar W.
Acta Neurobiologiae Experimentalis
|
2005
|
tom 65
|
nr 3
9

Influence of glutamate injection into unilateral nucleus accumbens shell on behavioral response elicited by ipsilateral stimulation of the mesolimbic system

51%
Plucinska K., Jerzemowska G., Podlacha M.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: Food intake is regulated not only by homeostatic requirements but also by emotional factors. The nucleus accumbens, particularly its shell region (AcbSh) is a part of the mesolimbic dopaminergic system which is responsible for a positive emotional aspect of various homeostasis-relevant stimuli. In the present work, we tested the AcbSh involvement in feeding behavior using an experimental paradigm specifically designed to assess motivational vs. motor aspect of food ingestion. METHODS: In rats (n=4), feeding was evoked by electrical stimulation of the midbrain ventral tegmental area (a somatodendritic region of mesolimbic system) and assessed quantitatively with the use of the latency to feed/stimulation frequency curve shift paradigm before and after ipsilateral glutamate injection (dose 2.0 µg dissolved in 0.5 µl of distillated water) into AcbSh (distillated water injection as a control, volume: 0.5 µl). RESULTS: Effect of ipsilateral glutamate injection into the AcbSh on behavioral response following the VTA stimulation was varied. In three rats the percentage reaction threshold did not change significantly and was approximated the baseline (not exceed ±10%). We observed an increase/decrease in the reaction threshold by only +0.31%, +2.85% and −1.90% in comparison to the water injection. In one rat the feeding threshold reaction was changed by significantly more than 10%. This significant increase was about +19.40% as compared to the baseline (water injection). CONCLUSIONS: Glutamate AcbSh activation – the major terminal area of the mesolimbic system does not affect the behavior induced by stimulation of the dopaminergic cells at the level of VTA. Presumably a different effect observed in one rat is dependent on the injected place within the AcbSh area. Research was financed by the Polish National Science Centre (NCN); decision no: DEC-2013/09/N/NZ4/02195
10

Mild laboratory stress elevates FOS expression in the amygdala and hypothalamus in rats high-responsive to novelty

45%
Majkutewicz I., Myslinska D., Jerzemowska G., Plucinska K., Wrona D.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
Responsiveness to novelty is often used as a measure of inter-individual vulnerability to stress loads and drug abuse. The aim of this study was to determine the relationship between individual behavioral profile and brain structures activation. Possible influence of stressful laboratory routines on manifestation of these individual differences was investigated. Male Wistar rats (n=21) were subjected to the novelty test and divided into high (HR) and low (LR) responders to a new environment according to median. Randomly chosen 6 LRs and 5 HRs rats were handled and carried out from the vivarium to the laboratory for nine days (carried group), remaining rats stayed in their home cages (control group, 5 HRs and 5 LRs). One week after the last carrying, an immunohistochemical detection of Fos protein in selected brain areas was performed. Carried HRs showed significantly higher Fos expression in all studied nuclei of the amygdala and most of the hypothalamic areas as compared to LRs and also to control rats. Carried LRs showed elevated density of Fos+ cells only in the stressrelated paraventricular and supraoptic hypothalamic nuclei. Surprisingly, inter-individual (HR vs LR) differences in brain activation was found in carried rats only. We conclude that mild stress evoked by some laboratory routines reveals constitutive differences between the individuals reflected by an increased activity of the amygdala and hypothalamus.
11

The effects of high frequency subthalamic stimulation on plasma corticosterone and pro-inflammatory cytokine concentrations in hemiparkinsonian rats

39%
Grembacka B., Listowska M., Glac W., Plucinska K., Jerzemowska G., Wrona D.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIMS: The subthalamic nucleus (STN) is the best target for deep brain stimulation (DBS) elevating motor symptoms in Parkinson’s disease (PD) patients. Although DBS is beneficial for patients with PD, it can also cause psychiatric and autonomic side effects of unknown etiology. Since the depression-like behavior is described after high frequency subthalamic stimulation (HFS-STN) in rats, it is important to examine impact of HFS-STN on hypothalamic-adrenal axis (HPA) activation and plasma proinflammatory cytokine levels. The aim of this study was to investigate the plasma corticosterone (CORT), TNF-α, IFN-γ and IL-6 levels following HFS-STN in hemiparkinsonian rats. METHODS: Unilateral, continuous HFS-STN (pulse width: 60 µs, frequency: 130 Hz, stimulation intensity: 30–115 µA, during 1 h. stimulation period) was provide in freely moving hemiparkinsonian rats. The model of PD was obtained by stereotactic microinjection of 6-hydroxydopamine into the right substantia nigra pars compacta. The blood samples were collected by heart puncture after the electrical or sham stimulation. The CORT level in plasma was measured by radioimmunoassay method, while concentrations of cytokines were quantified using ELISA method. RESULTS: We found that HFS-STN applied in hemiparkinsonian rats significantly increase plasma CORT level (t27=2.31, P≤0.05) in comparison to SHAM control group). We also observed an increase in IFN–γ (t27=2.89, P≤0.01) and TNF-α (t27=2.88, P≤0.01) concentrations, while the IL-6 level decrease (t27=3.38, P≤0.01) following HFS-STN. CONCLUSIONS: These data shows that HFS-STN influence endocrine and immune parameters in peripheral blood in hemiparkinsonian rats. One hour, continuous HFS-STN activated HPA axis (measured by plasma corticosterone level) and elevated concentrations of pro-inflammatory cytokines. Our results suggest that HFSSTN provokes peripheral immune and endocrine effects similar to observed in behavioral depression.
12

Lesion and stimulation of the ventral tegmental area increases cholinergic activity in the rat brain

39%
Majkutewicz I., Cecot T., Jerzemowska G., Trojniar, Jaskulski M., Wrona D.
Acta Neurobiologiae Experimentalis
|
2010
|
tom 70
|
nr 1
Our previous study indicated that unilateral lesion of the ventral tegmental area (VTA) facilitates contralateral VTA stimulation-induced feeding or exploration. The present study was aimed to determine the possible role of the central cholinergic systems in this effect. Immunohistochemistry for choline acetyltransferase (ChAT) was used to measure the number of active cholinergic neurons in their major groups (Ch1–Ch6) and in striatal regions in rats subjected to unilateral electrocoagulation and contralateral VTA electrical stimulation (L/S group) in comparison to the unilaterally stimulated (S), unilaterally lesioned (L) and sham (Sh) groups. The study showed that unilateral VTA lesion increased (as compared to Sh group) the number of ChAT+ neurons in the Ch1–Ch3 and unilateral VTA stimulation increased the number in the Ch1 and the ventral pallidum only. The most sensitive to these changes in the mesolimbic system were cholinergic structures providing hippocampal afferentation. Surprisingly, there was no significant increase in the number of ChAT+ neurons in the L/S group. The obtained results did not confirm any evident influence of the cholinergic systems on the VTA lesion-induced facilitation of the behavioral response evoked by contralateral VTA stimulation.
13

NMDA receptor modulation of the pedunculopontine tegmental nucleus decreases the number of tyrosine hydroxylase positive cells in the ventral tegmental area and substantia nigra pars compacta in rats

39%
Plucinska K., Piwka A., Ptaszek K., Podlacha M., Orzel-Gryglewska J., Jerzemowska G.
Acta Neurobiologiae Experimentalis
|
2019
|
tom 79
|
nr Suppl.1
INTRODUCTION: Two notable targets of the pedunculopontine tegmental nucleus (PPN) circuitry, the substantia nigra (SN) and the ventral tegmental area (VTA), are implicated in locomotion and reward processing. A dysfunction of these regions occurs in Parkinson’s and related disorders as well as in various psychiatric conditions, and over the course of normal aging AIM(S): In the present study, we were interested in understanding NMDA-receptors involvement in the interactions between the PPN and SN/VTA midbrain complex. In order to obtain more insight into this process, we analyzed the number and the distribution of midbrain tyrosine hydroxylase positive cells (TH+). METHOD(S): All rats were implanted with bilateral stimulating electrodes in the VTA and with bilateral guide cannulas for intracerebral injections into the PPN. Immunohistochemistry for TH+ was used to measure the number of active dopaminergic neurons in midbrain (VTA-SN) of rats subjected to unilateral VTA electrical stimulation and local injection of MK‑801 (5 μg) or NMDA (3 μg) to the contralateral or ipsilateral hemispheres into the PPN (4 experimental groups). The control brains were from rats in which only the 14‑day unilateral electrical VTA‑stimulation was performed (control group). RESULTS: Immunohistochemical analysis revealed a decrease in the number of TH+ cells in the midbrain. When the main subdivisions of the VTA/SN were subjected to a separate analysis, a significantly lower number of TH+ cells were found in all experimental groups in the PBP (parabrachial pigmented nucleus), PB (paranigral nucleus) and SNc (SN, pars compacta), as compared to the control group. CONCLUSIONS: The level of NMDA receptor arousal in the PPN regulates the activity of the midbrain dopaminergic cells. FINANCIAL SUPPORT: The research was funded by the Polish National Science Center; decision no: DEC‑2013/09/N/NZ4/02195 and by the Faculty of Biology, University of Gdansk, Poland.
14

Lesion and stimulation of the mesolimbic motivational dopamine system influence blood peripheral lymphocyte percentage in rats differing in locomotor activity

39%
Plucinska K., Grembecka B., Glac W., Myslinska D., Jerzemowska G., Wrona D.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
The mesolimbic dopamine system, that plays a pivotal role in emotionally motivated behaviours, originates in the ventral tegmental area (VTA) and sends major projections to the nucleus accumbens (Acb). In the present work, we assessed the effects of manipulation in the mesolimbic dopaminergic system identified with the “brain reward system” on the percentage of peripheral blood lymphocytes in freely moving rats differing in locomotor activity to novelty (high responders; HR or low responders; LR). T and B lymphocyte populations were determined by flow cytometry (CD3-FITC/CD45RA-PC7). Peripheral blood was collected from rats exposed to chronic 14 day VTA electrical stimulation to obtain feeding or exploration response (constant current 0.1 ms duration, cathodal pulses, 50 Hz, 30-min daily session) and with a lesion electrode aimed at Acb: 3 weeks after electrode implantation, after 2 weeks of chronic VTA stimulation, on the 2nd day after the Acb lesion (2mA for 15s) and on the 14th VTA stimulation day following the Acb lesion. As compared to the respective sham animals, chronic stimulation of the VTA caused significant (p<0.05) decrease in T cell percentage in both HR (33.32 ± 5.39% vs 48.04 ± 7.45%) and LR (44.99 ± 2.94% vs 51 ± 3.57%) animals. In addition, significantly (p<0.05) lower level of T cell percentage in HR animals (40.13±3.64% vs 50.94 ± 5.87%) rats were observed. On the 14th VTA stimulation day following the Acb lesion, significant (p<0.05) decrease in B cell percentage in both HR (12.47 ± 3.55% vs 19.45 ± 1.58%) and LR (7.09 ± 2.48% vs 16.5 ± 3.46%) animals was observed. On the other hand, chronic stimulation of the VTA (20.99 ± 3.38% vs 17.65 ± 0.49%) and Acb lesion (25.14 ± 2.48% vs 20.62 ± 0.85%) caused significant increase (p<0.05) in B cell percentage in HR animals. The results emphasize the importance of individual differences (HR vs LR) in the influence of the mesolimbic motivational system on blood lymphocyte distribution.
15

Electrical stimulation of the medial septal nucleus increases cytotoxic activity of NK cells in the spleen and peripheral blood in rats

33%
Plucinska K., Grembecka B., Glac W., Budtke P., Jerzemowska G., Myslinska D., Orlikowska A., Wrona D.
Acta Neurobiologiae Experimentalis
|
2009
|
tom 69
|
nr 3
The medial septal nucleus (MS) is a forebrain limbic structure involved in learning and memory mechanisms. In previous study we found that electrolytic lesion of the medial septal nucleus caused depression of the peripheral blood natural killer cell cytotoxicity (NKCC) and the leukocyte number. In the sham operated group mere insertion of electrodes into the MS evoked transient NKCC enhancement, probably resulting from mechanical MS stimulation. To check this effect in the present study, we evaluated both spleen and blood NKCC (51Cr-release assay) and large granular lymphocytes (LGL) number (a morphological method) after chronic electrical stimulation (constant current 0.1 ms duration cathodal pulses delivered at a frequency of 50 Hz during 30-min daily session for 14 consecutive days) of MS in conscious, freely moving rats (n=12). Additionally, peripheral blood leukocyte, lymphocyte and neutrophile number was mesaured. Chronic MS stimulation caused signifi cant blood NKCC augmentation and LGL number (25.86 ± 9.31% vs. 15.75 ± 4.75%, P<0.01) in comparison to the sham group (n=13). No signifi cant effect was found in the spleen (27.14 ± 9.99% vs. 28.58 ± 8.04%). A week after termination of the stimulation procedure all measured parameters returned to the baseline. The results obtained indicate that such limbic structure as medial septum enhances antitumor and antiviral function and number of NK cells.
16

Functional reorganization of the mesolimbic system after its terminal area destruction influences blood leukocytes and their subsets

33%
Plucinska K., Grembecka B., Myslinska D., Listowska M., Glac W., Jerzemowska G., Badtke P., Wrona D.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr Suppl.1
Contralateral nucleus accumbens shell (AcbS) lesions (Contra group) impaired (by about 20%) and ipsilateral AcbS lesions (Ipsi group) facilitated (by about 30%) motivational aspects of ventral tegmental area (VTA) stimulation-induced feeding or exploration which manifested as respective alterations in latency to reaction. Present work was aimed to examine how this motivational reorganization of AcbSVTA circuitry affect on blood leukocytes and their subsets (morphological method). As compared to the respective sham animals, the chronic VTA stimulation and unilateral lesion of the AcbS caused a significant decrease in total leukocyte and lymphocyte numbers in Ipsi and Contra groups. Both groups showed also significant decreases in total leukocyte and lymphocyte numbers on the 2nd day after unilateral lesion of the AcbS. On the 14th VTA stimulation day following unilateral lesion of the AcbS total leukocyte and a large granular lymphocyte (LGL) number was higher in Ipsi group in relation to Contra group and in comparison with the respective sham group. Increased motivational drive associated with facilitation reactivity of the ipsilateral VTA to lesioning AcbS enhance total leukocyte number, especially LGL cells that are critical to the innate immune system.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.