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The variability of pollen grains of 16 species from genus Rosa L. was studied (i.e. Rosa agrestis, R. canina, R. dumalis, R. gallica, R. inodora, R. jundzillii, R. kostrakiewiczii, R. majalis, R. micrantha, R. mollis, R. pendulina, R. rubiginosa, R. sherardii, R. tomentosa, R. villosa, and R. zalana). The material came from 107 native localities of those species in Poland. The measurements are based on at least 30–50 randomly selected mature pollen grains per specimen. In total, 3510 pollen grains were examined. They were analysed for 8 quantitative features, i.e. length of polar axis (P), length of equatorial axis (E), exine thickness on the pole (Exp), exine thickness at the equator plane (Exe), length of ectocolpi (Le), P/E ratio, and relative thickness of exine (Exp/P and Exe/E ratio). Statistically significant differences were found among the examined species with regard to all analysed pollen features. The pollen and ectocolpi dimensions (P, E and Le) were largest in R.gallica (35.9, 28.1, and 28.0 μm, respectively) and smallest in R. majalis (27.0, 20.2, and 21.2 μm, respectively). The mean coefficients of variability of the pollen features measured can be used to arrange the examined rose species from the least to the most variable as follows: R. pendulina, R. villosa, R. jundzillii, R. inodora, R. canina, R. rubiginosa, R. dumalis, R. gallica, R. agrestis , R. micrantha, R. zalana, R. tomentosa, R. sherardii, R. majalis, R. kostrakiewiczii and R. mollis. The obtained data failed to confirm fully both the division of the Rosa genus currently in force in taxonomy into sections as well as relationships among the examined species from the Caninae section. In addition, values of morphological characters of the same species may differ considerably from one another. The extent of these differences indicated that it was necessary to measure large numbers of pollen grains in order to obtain accurate biometric data.
We studied the chronosequence of six Scots pine (Pinus sylvestris L.) plantations (6-, 9-, 11-, 15-, 17-, and 20-years-old) to examine the changes in leaf area index (LAI) over stand age. The study site was located on a mine spoil heap (outer dumping ground) in the Belchatow lignite open-cast mining district (central Poland). The main goal of the study was to analyze LAI changes over stand age in a chronosequence of young Scots pine stands and to test the relationship between LAI estimates derived from a LAI-2000 Plant Canopy Analyzer and site-specific allometric equations. In addition, we tried to determine whether LAI estimated by a LAI-2000 PCA can be used to accurately estimate forest biomass. We hypothesized that LAI-2000 PCA underestimates LAI of the stands, and that stand age (and linked stand parameters) may influence the range of the hypothetical underestimation due to changes in biomass allocation. Our study revealed that LAI was highly dependent upon stand age and tree density (p<0.0001) regardless of the way how LAI was determined. Moreover, we found that LAI estimated by LAI-2000 PCA significantly correlates with stand biomass per area; the highest coefficients of determination were found for total aboveground biomass, aboveground woody biomass, and stem biomass. This means that data obtained by LAI-2000 PCA are good predictors of stand biomass in the young stages of stand development. In contrast to our prior assumption, the results showed that LAI-2000 PCA overestimates leaf area index. The relative differences between the values obtained with LAI-2000 and those calculated on the basis of the site-specific allometric equations increase with age. This may reflect changes in crown architecture over age caused by enlarging tree dimensions and over - crowding of trees leading to deterioration of light conditions inside the canopy.
In addition to physical defenses, chemical defenses are the most effective way to protect plants from adverse biotic factors (phytophagous insects, other herbivores and pathogenic fungi). This requires extra effort from plants to produce secondary defense metabolites at the expense of production of primary metabolites directly linked to the growth and development of plants. There are three main groups of defensive compounds (alkaloids, phenolic compounds and terpenoids). All defensive compounds are rich in carbon. Depending on the chemical formula, carbon makes up from about 40% to over 85% of the molecular weight of various defense compounds. It is not possible to calculate the total carbon mass accumulation in all defense compounds. In this paper we discuss the content of defensive compounds and carbon with respect to defense strategy of plants, functional groups of woody species (coniferous and deciduous trees), tree species, tree biomass components (leaves, branches, bark, roots, etc.) and many other internal (age of trees, age of leaves, stage of development, origin, etc.) as well as external factors, related to soil and climatic conditions
The study was carried out in 39- and 43-year-old stands of noble fir (Abies procera Rehder) grown in the Rogów Arboretum of the Warsaw University of Life Sciences (Poland). The main objectives of our study were (1) to estimate stem volume over bark of noble fir grown out of its natural range, (2) to develop suitable allometric equations for estimating aboveground woody biomass components and (3) to estimate aboveground woody biomass components using site-specific allometric equations and to compare them with biomass estimated using allometric equations developed in stands grown within natural range of noble fir. The study showed that the mean DBH of trees was 20.14 cm in the younger stand and 22.25 cm in the older stand. The basal area of the 39-year-old stand was 49.01 m2·ha-1 and 43-year-old stand was 47.53 m2·ha-1. Based on the developed equation stem volume over bark was 374.87 m3·ha-1 and 356.24 m3·ha-1 in the 39- and 43-year-old stands, respectively. Based on the developed site-specific allometric equations total aboveground woody biomass in the 39-year-old stand was 189 Mg·ha-1 whereas in the 43-year-old stand it was 184 Mg·ha-1. Branch biomass in both stands equaled 19.9% of total aboveground wood biomass. Total aboveground woody biomass, estimated by allometric equations published by Ter-Mikaelian and Korzukhin [1997], equaled 233 Mg·ha-1 and 228 Mg·ha-1 in the 39- and 43-year-old stands, respectively. This means that the aboveground woody biomass is overestimated by ca. 23% in comparison with biomass estimated by our site-specific allometric equation. Generally, the existing equations published by Ter-Mikaelian and Korzukhin [1997] overestimated total aboveground woody biomass and stem biomass, while branch biomass was underestimated across all tree sizes compared to directly obtained biomass data.
Ectomycorrhizal (ECM) fungi and their symbiotic associations with forest trees are among major drivers of key ecosystem functions such as carbon and nitrogen cycling, plant nutrient and water uptake from soil, plant-plant facilitation/competition and diversity regulation via common mycorrhizal networks. Through their functional traits and interactions with both abiotic and biotic environment, they also significantly affect the process of open land colonization by trees as well as vegetation succession coupled with soil and ecosystem development. Here we review the role of ECM fungi in the early primary and secondary succession following major anthropogenic disturbances. Based on the examples of mine spoils and post-agricultural lands, we demonstrate key ecosystem services provided by ECM fungi in the processes of forest restoration. We point out ecological mechanisms and adaptations which underpin ECM fungal community successional interactions, particularly life histories, dispersal, spatial structure, host preferences, and sensitivity to environmental filters. We emphasize the need of better understanding the role of ECM fungi in the forest restoration practice as it seems crucial for afforestation success and biodiversity rehabilitation. Thus, ectomycorrhizal traits should be a prime consideration in afforestation and carbon sequestration polices, sustainable forest management and biodiversity conservation practices.
The objective of this study was to investigate the ranges of intra- and interindividual variability on the example of R. canina. For this purpose, four flowers were collected randomly (72 flowers in total)from 18 wild shrubs of R. canina growing in one population in Poznań (Poland)and then, from each flower, 50 correctly formed pollen grains (200 pollen grains per each individual)were selected. Inter- and intraindividual pollen grain variability was characterised based on 3600 pollen grains. They were analysed for seven quantitative features, i.e. length of polar axis (P), equatorial diameter (E), thickness of the exine along the polar axis (Exp), length of ectocolpi (Le) and P/E, Exp/P, and Le/P ratios. Our study revealed highly significant differences among flowers of the particular R. canina individuals with respect to all pollen grain biometrical features. In addition, it showed that the assessment of the full range of variability in pollen grain biometric features within one individual (shrub)was more reliable if we examined several pollen grains from several flowers than for the same number of pollen grains derived from a single flower. We also found statistically significant differences among particular individuals in all pollen grain features. This proves that in order to well characterise a population of a given species from the point of view of palynology, the plant material should derive from a possibly numerous number of individuals (shrubs).
The purpose of this study was to examine how stand age affects fine root biomass and morphology in different stages of first generation Scots pine forest development in post-agricultural fields. Stands of different ages (6-, 10-, 16-, 28-, and 47-yr-old) were studied at the same time to provide data on biometrical fine root features, i.e. biomass, length, surface area, volume, number of tips, root tip density, specific root tip density, specific root area, specific root length and fine root tissue density. Soil cores from the upper 20 cm of soil were used for the study. The results of the study show that fine root characteristics did significantly differ among stands of different age. Fine root biomass ranged from 0.9 Mg ha–1 (6-yr-old stand) to 2.3 Mg ha–1 (47-yr-old stand), whereas coarse root biomass ranged from 0.2 Mg ha–1 to 3.2 Mg ha–1, respectively. Fine root biomass in the older stands (10–47-yr-old) remains constant and is ca. 4 times higher than in the youngest stand (6-yrs-old). This shows that the fine root biomass of Scots pine in the upper soil horizons reached a constant biomass at a younger stand age than found in previously published papers, although at the same stage of stand development, i.e. canopy closure. Fine root length, surface area and volume expressed on per stand area basis were significantly different among stands; the highest values were found in the 10-yr-old stand, during the time of canopy closure. This means that stand age (i.e. age of trees in pure even-aged monocultures) is not a major factor influencing the fine root dynamics, instead stage of development and other stand and habitat characteristics may play an important role. Moreover, we found significant linear relationships among stand age and fine root length, surface area and number of fine root tips expressed on a per tree basis. Our study showed that stand age affects both fine root biomass and morphology in Scots pine forests when growing on post-agricultural fields. The differences revealed in our study indicate high plasticity of Scots pine fine roots in response to stand changes over age.
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