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Variety of traits important in diagnostics and epidemiology of pathogenic microorganisms may change due to antibiotics. Movement ability, that is characteristic for every serovar except from Salmonella Gallinarum-Pullorum, is important to salmonellas. In own experiments using semi-fluid MSRV medium, it was found that a decrease in salmonella sensibility to selected antibiotics and chemiotherapeutics due to passage might lead to weakening of its movement ability. Movement ability of all strains (S. Enteritidis, S. Dublin, S. Typhimurium) after passage with amoxycillin, neomycin, Colistin and enrofloxacin became weakened as compared to results achieved before passage. The strongest inhibition of movement ability was most often observed in strains after passage on medium with Colistin. It seems to be associated with the action mechanism of the antibiotic. Colistin injuries cellular membranes, where flagella (active motoric organ of Salmonella) are anchored. Appearance of drug-resistance as a result of passage at the presence of antibiotics may cause variability of biochemical properties of Salmonella rods and leads to weakening of movement ability of ciliated Salmonella.
In twenty broiler flocks the following antibodies against common viruses were evaluated: chicken anaemia (CAV), infection bursal disease (IBDV), infection bronchitis (IBV) and reoviruses. The antibodies against Marek’s disease virus (MDV) were additionally examined. The presence of antibodies against CAV, IBDV and IB was determined by comercial ELISA kits and against reoviruses by ELISA performed according to the modified method of Slaght. The antibodies against CAV occurred in 7 flocks, the percentage of positive sera was 5% to 22%. All the sera showed the presence of IBDV antibodies. The antibodies against IBV were found in 5 flocks. In 7 flocks 80% of sera were positive. The antibodies against reoviruses were found in 90% of the flocks examined. All sera were positive in 8 flocks and negative in 2 flocks. In 6 flocks antibodies against MDV were found in 5% to 40% of the chickens.
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