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Nietypowe sposoby rozmnażania rzęsistka pochwowego

100%
Microphotos (1-5) from multiple division of T. vaginalis grown on Roiron medium are presented. The author encountered similar multiplication patterns in samples collected directly from patients. The sections were stained according to modified May-Grunwald-Giemza technique developed in the Institute of Hygiene, Medical School in Białystok.
Out of 192 water samples from 4 pools, including 84 (56 liters) from 1956 and 108 (68 liters) from 1966, none showed the presence of T. vaginalis.
The in vitro studies have shown a high sensitivity of T. vaginatis strains to the spermicidal drug Afro of Polish production. Among 36 strains under examination there were individual differences in the sensitivity to the drug. Moreover, the in vivo experiments performed in guinea pigs disclosed that Afro when given intravaginally prevented the incidence of trichomonal invasion in these animals. In the Ist group of guinea pigs receiving the drug 3 hours before the infection none of the 5 animals developed the symptoms of trichomonadosis. In the IId group of 5 animals that were administered Afro 6 hours before the infection the prophylactic effect was found in 4 cases, and in the IIId group where the drug was given 12 hours prior to infection the trichomonadosis symptoms did occur in 2 out of 5 guinea pigs. The in vivo experiments made on guinea pigs require still further confirmation on larger material.
Estimation of virulence of T. vaginalis strains injected subcutaneously to mice was done. T. vaginalis strains were obtained from the urinogenital tracts of patients with different clinical forms of trichomonosis. The virulence of T. vaginalis strains measured by infiltration size was found to depend upon a clinical form of trichomonosis. It was moreover shown that the cutaneous infiltration size is proportional to a dose of Trichomonas administered subcutaneously. Histopathological analysis of cutaneous infiltration itself is not decisive as to the strains virulence, yet in addition to macroscopic examination it may be a criterium in the studies on strain pathogenicity.
It has been shown that glycoprotein and polysaccharide fractions obtained from T. vaginalis cells can be employed as antigens in serodiagnosis of trichomonosis. This refers especially to the polysaccharide fraction because of its easy availability, solubility in aqueous solutions, high diagnostic specificity and sensitivity. It has been stated that the diagnostic value of polysaccharide antigen of T. vaginalis, in occult trichomonosis in men particularly, is higher than the effect of the secretion examination by means of culture, even after the prostatic gland massage.
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Wrażliwość Trichomonas vaginalis na wysychanie

45%
Twelve strains of T. vaginalis were tested for their sensitivity to dessication at room temperature. Each of them, in the quantity of 0.5 cm³ of 36 hrs old culture (containing 2.5 mln cells) was placed in 5 sterilized tubes containing 2:5 cm³ of: sterilized Roiron medium, urine, 0.9% NaCl, tap water and distilled water. After shaking, the suspension of Trichomonas of each tube in the quantity of 0.15 cm³ was placed on depression slides. The slides were then placed in sterilized, not tightly covered Petri dishes, which were kept at room temperature. The suspension of 3 slides was examined under microscope and inoculated on Roiron medium prior to complete dessication. The same procedure was followed with respect to the fourth slide but only after the suspension had, dried. The suspension remaining in the tube (2.4 cm³) served as control. The experiment showed that in a drop of 0.9% NaCl, urine and Roiron medium, Trichomonas remains active for 120 min. When transferred to Roiron medium within that time, Trichomonas reproduces in a normal way which was not observed after a transfer from the suspension dried up. In a drop of water Trichomonas loses its ability to reproduce within a time shorter than 120 min. The results speak in favour of a possibility of a non-sexual infection with Trichomonas.
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