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1

Zieleń miejska jako wielofunkcyjna przestrzeń publiczna na przykładzie Parku Śląskiego

100%
Chojecka A.
Teka Komisji Architektury, Urbanistyki i Studiów Krajobrazowych
|
2013
|
tom 9
|
nr 2
2

IV Ogolnopolska Konferencja Hydromikrobiologiczna [Mikolajki, 21-24 V 2006 r.]

63%
Chojecka A., Adamczewski T.
Wiadomości Ekologiczne
|
2006
|
tom 52
|
nr 4
212-215
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Ocena mikrobiologiczna skuteczności dezynfekcji chemiczno-termicznej bielizny szpitalnej zanieczyszczonej krwią

51%
Rohm-Rodowald E., Jakimiak B., Podgorska M., Chojecka A.
Roczniki Państwowego Zakładu Higieny
|
2010
|
tom 61
|
nr 3
W procesie prania bielizny szpitalnej zanieczyszczonej krwią powinna być stosowana dezynfekcja termiczna. Obecnie jest coraz więcej bielizny szpitalnej wykonanej z materiałów bawełniano-poliestrowych, które nie mogą być poddane działaniu wysokiej temperatury-dezynfekcji termicznej. Przeprowadzanie dezynfekcji chemiczno-termicznej w coraz niższych temperaturach stwarza drobnoustrojom większą możliwość przeżywania procesu prania. Celem badań było przygotowanie nowej metody oceny mikrobiologicznej chemiczno-termicznej dezynfekcji w procesie prania bielizny szpitalnej zanieczyszczonej krwią. W badaniach określano działanie bakteriobójcze środków przeznaczonych do chemiczno-termicznej dezynfekcji w procesie prania symulując w laboratorium procesy prania zgodnie z metodą PZH -DF/05/03. W celu określenia efektu bakteriobójczego dla bielizny szpitalnej zanieczyszczonej krwią zastosowano bawełniane nośniki zaszczepione Enterococcus faecium oraz substancje obciążające - wysokie stężenia albuminy i/lub erytrocytów baranich, imitujące ludzką krew. Badania wykazały; że działanie bakteriobójcze środków do chemiczno-termicznej dezynfekcji bielizny szpitalnej o dużym zanieczyszczeniu organicznym (krew; itp.) powinny być oceniane metodą nośnikową w następujących warunkach: organizm testowy - Enterococcus faecium, substancje obciążające - 6g/l albuminy dodawanej do preparatu.
4

Wrażliwość wybranych szczepów używanych do oceny biobójczej skuteczności preparatów dezynfekcyjnych oraz antybiotykoopornych szczepów na rozpuszczony w 2-propanolu chlorek didecylodimetyloamoniowy

45%
Chojecka A., Wiercinska O., Rohm-Rodowald E., Kanclerski K., Jakimiak B.
Medycyna Doświadczalna i Mikrobiologia
|
2015
|
tom 67
|
nr 1
5

Znaczenie pomp efflux w wielolekowej oporności Gram-ujemnych bakterii

45%
Wiercinska O., Chojecka A., Kanclerski K., Rohm-Rodowald E., Jakimiak B.
Medycyna Doświadczalna i Mikrobiologia
|
2015
|
tom 67
|
nr 1
6
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Effect of adaptation process of Pseudomonas aeruginosa to didecyldimethylammonium chloride in 2-propanol on bactericidal efficiency of this active substance

45%
Chojecka A., Wiercinska O., Rohm-Rodowald E., Kanclerski K., Jakimiak B.
Roczniki Państwowego Zakładu Higieny
|
2014
|
tom 65
|
nr 4
Background. Microorganisms are characterized by two types of resistance innate and acquired. Innate resistance is associated with the construction of the surface structures. Wide use of active substances as antimicrobial compounds, especially in inhibitory concentrations, may promote the acquisition of bacterial resistance to these substances in the process of adaptation. Objective. The aim of the study was to determine changes in efficiency of didecyldimethylammonium chloride in 2-propanol (CMAP) against the Pseudomonas aeruginosa strains, which were adapted to this active substance. Materials and Method. Adaptation studies were conducted using two strains: P. aeruginosa ATCC 15442 (PA), which is used in estimation of biocide efficiency and tetracycline-resistant P. aeruginosa ATCC 47085 (PAO-LAC) strain. These strains were adapted to the active substance Bardac22: 50% v/v didecyldimethylammonium chloride in 20% v/v 2-propanol (CMAP) according to the National Institute of Hygiene procedure. After adaptation, obtained isolates were classified to three groups and passaged to solid media: A – strains unadapted passaged onto slant medium without active substance, control group; B – strains with adaptive resistance passaged onto slant medium with 375 mg/l CMAP; C – strains with adaptive resistance passaged onto slant medium without CMAP. Changes in susceptibility of examined strains were determined on the basis of minimum inhibitory concentrations (MICs) by broth dilution method. The minimum bactericidal concentrations (MBCs) were determined by subculture P. aeruginosa strains on solid media without CMAP. The efficiency of CMAP against isolates obtained after adaptation processes was evaluated by using phenol coefficient (PC). Results. There were no differences in the adaptation process between two strains of P. aeruginosa: PA and PAO-LAC. Both isolates obtained after the adaptation process was characterized by approximately 6-8 fold higher MICs compared to the MICs of these strains before the adaptation. Strains passaged to a solid media characterized a variable sensitivity to CMAP. As compared to a control group A, the isolates of PA and PAO-LAC from group B and isolate PA from group C exhibited the highest and stable insensitivity (MIC from > 700 to >1000 mg/l) to 48-49 passages. Isolates from group C of PAO-LAC maintained insusceptibility up to 20th passage (MIC >375 mg/l). There were no statistically significant changes in the CMAP bactericidal efficacy against isolates of reduced sensitivity. Conclusions. Adaptation of P. aeruginosa strains to didecyldimethylammonium chloride in 2-propanol does not significantly change bactericidal efficacy of this active substance against isolates with reduced sensitivity. Antibiotic-resistant strain PAO-LAC showed a similar adaptability and a similar sensitivity to the CMAP as a strain PA used to assess the effectiveness of disinfectants.
7

Ocena aktywności bójczej preparatów przeznaczonych do dezynfekcji chemiczno-termicznej bielizny szpitalnej w świetle nowej normy europejskiej pn-en 16616: 2015-10. Dezynfekcja chemiczno-termiczna tekstyliów

45%
Tarka P., Chojecka A., Paduch O., Nitsch-Osuch A., Kanecki K.
Postępy Mikrobiologii
|
2017
|
tom 56
|
nr 1
8
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Neutralization efficiency of alcohol based products used for rapid hand disinfection

45%
Chojecka A., Tarka P., Kierzkowska A., Nitsch-Osuch A., Kanecki K.
Roczniki Państwowego Zakładu Higieny
|
2017
|
tom 68
|
nr 4
Background. Alcohols are the most commonly used active substances in preparations for quick hand disinfection. They should be bactericidal in very short contact time. PN-EN 13727 + A2: 2015-12 standard, for testing hygienic and surgical handrub disinfection preparations, provides mandatory test conditions of disinfectants in contact times with the range of 30 s to 60 s (hygienic handrub disinfection) and 60 s to 5 min (surgical handrub disinfection). A short contact times for hand hygiene products require a short time of neutralization process. For contact times less than or equal to 10 minutes, the estimated neutralization time is 10 s ± 1 s. Neutralization is a process that abolishes the action of disinfectants. Correct application of this process allows for proper use of disinfectants in practice and its biocidal effect. Objectives. Verification of the effectiveness of 10-second neutralization time of alcohol based preparations for hygienic handrub disinfection. Materials and Method. Neutralization of two products with different ethanol content (89% and 70%) for hygienic handrub disinfection according to PN-EN 13727 + A2: 2015-12 was investigated. The effectiveness of the neutralizer was assessed by determining toxicity of neutralizer, activity of residual effects of the tested products and their derivatives produced during neutralization (10 s) for test organisms (Staphylococcus aureus ATCC 6538; Pseudomonas aeruginosa ATCC 15442; Enterococcus hirae ATCC 10541; Escherichia coli K12 NCTC 10538). Results. The 10-second neutralization time was sufficient to eliminate the residual activity of products for hygienic handrub disinfection with differentiated ethanol concentration. The neutralizer used did not show toxicity to bacteria and did not produce toxic products with tested preparations after neutralization. Conclusions. The use of 10-second neutralization time allows in a precise way designate the contact times for hygienic handrub disinfection products.
9
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Glucoprotamin antimicrobial activity against selected standard antibiotic-resistant bacteria and reference strains used in the assessment of disinfection efficacy

45%
Chojecka A., Wiercinska O., Rohm-Rodowald E., Kanclerski K., Jakimiak B.
Roczniki Państwowego Zakładu Higieny
|
2015
|
tom 66
|
nr 3
Background. The ability of bacteria to develop common mechanisms of resistance to antibiotics and disinfectants raises doubts about the effectiveness of disinfection processes. Glucoprotamin (GP) is an antimicrobial active substance which is widely used to the disinfection in medical area. Objective. The aim of study was to compare GP’s effectiveness with susceptibility of reference strains used for the evaluation of bactericidal efficacy of disinfectants Staphylococcus aureus (S. aureus); Pseudomonas aeruginosa (P. aeruginosa) and standard antibiotic-resistant strains: meticillin-resistant S. aureus (MRSA) and tetracycline-resistant P. aeruginosa (PAO-LAC). Materials and Methods. Minimum inhibitory concentrations (MICs) of GP and minimum bactericidal concentrations (MBCs) against tested strains were evaluated by serial broth dilution technique. GP’s efficiency was examined according to qualitative (phenol coefficient GP-PC) and quantitative (EN 1040: 2006) test methods. Results. Gram-negative strains were more tolerant to GP than Gram-positive strains among tested strains. MRSA and S. aureus exhibited similar susceptibility to GP. PAO-LAC had significantly lower susceptibility to GP than P. aeruginosa (P≤0,05). There were no differences in GP efficiency against these strains based on GP-PC. According to PN-EN 1040: 2006 standard average obligatory reduction ≥ 5 log10, was demonstrated in the active concentration of GP (84 mg/l) at obligatory 5 min contact time for PAO-LAC and P. aeruginosa. The differences in basis bactericidal activity between PAO-LAC and P. aeruginosa were obtained in the active concentration at 10 and 15 min contact time (P≤0,05). Conclusions. Variation in a susceptibility of reference strains and antibiotic-resistant standard strains has no meaning at used clinically GP concentrations, which are higher than concentration causing basis bactericidal activity of GP.
10
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Effect of different drying conditions for the viability of Candida albicans present on carriers

39%
Rohm-Rodowald E., Chojecka A., Jakimiak B., Wiercinska O., Ziemba B., Kanclerski K.
Roczniki Państwowego Zakładu Higieny
|
2014
|
tom 65
|
nr 1
Background. Problems in substantial under recovery of Pseudomonas aeruginosa and Candida albicans from carriers have been demonstrated for laboratories performing phase 2, step 2 efficacy tests on disinfectants relative to levels required by the EN 13697 standard. It was thus necessary to determine recoveries of these microorganisms following procedural losses incurred during drying and to optimise drying conditions such that recoveries then complied with the standard. Objectives. The aim of the study was to establish optimal drying conditions for the recovery of Candida albicans ATCC 10231 from carriers. Materials and Methods. The evaluation was performed according to the EN 13697:2001 standard procedure. A test suspension of Candida albicans and interfering substance were inoculated onto the surface of carriers (2 cm diameter stainless steel discs) and then dried under different conditions consisting of: a 37°C incubation with and without an incubator fan as well as at 23°C (room temperature) in a laminar air flow cabinet. Carriers were dried until the surfaces appeared visibly dry and the number of surviving organisms then recovered from the surface were quantified. The following were calculated for colony forming units (cfu); N (log10 cfu in a 0.05 ml test suspension), NC (the control log10 cfu in neutralizing medium), Nts (cfu numbers remaining on the surface) and the N-NC difference which should not exceed 2 log10 when microorganism recoveries are adequate and without any toxicity effects of the neutralising medium. Experiments was conducted using validating procedure (NC) which is performed with distilled water. Results. Drying at 37°C adversely affected the survival of Candida albicans and prevented the levels of microbial recovery from carriers to reach those specified by the EN 13697 standard. However, drying at around room temperatures of 23°C reduced Candida albicans mortality and increased recoveries from the carrier to levels compliant with the standard, where the N-NC differences were not greater than 2 log10. Conclusions. The viability of Candida albicans ATCC 10231 is sufficiently improved when carriers are dried at 23°C, even if the drying time exceeds 60 minutes. The density of the initial test suspension (N) should also be increased.
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