Wyniki wyszukiwania

1

Kwasy rybonukleinowe oraz fitohromy w rozwijających się ziarniakach zbóż

100%

Weidner S.

Postępy Nauk Rolniczych

|

1982

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tom 29

|

nr 4

2

Role of gibberellins and cytokinins in regulation of germination during development and ripening of Triticale caryopses

100%

Weidner S.

Acta Societatis Botanicorum Poloniae

|

1984

|

tom 53

|

nr 2

3

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Przedsprzętne porastanie ziarniaków zbóż i jego regulacja

100%

Weidner S.

Postępy Nauk Rolniczych

|

1987

|

tom 34

|

nr 1-2

4

II. Przedsprzętne porastanie ziarniaków zbóż i jego regulacja

100%

Weidner S.

Postępy Nauk Rolniczych

|

1992

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tom 39

|

nr 5-6

5

The role of the embryoless parts of triticale caryopses in inhibiting precocious germination and transcription in the embryo during development and maturation of caryopses

100%

Weidner S.

Acta Societatis Botanicorum Poloniae

|

1988

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tom 57

|

nr 4

6

Aktualne poglądy na kiełkowanie ziarniaków zbóż ze szczególnym uwzględnieniem biosyntezy kwasów nukleinowych

100%

Weidner S.

Postępy Nauk Rolniczych

|

1981

|

tom 28

|

nr 5

7

Mechanizm działania kwasu abscyzynowego

88%

Weidner S.

Postępy Nauk Rolniczych

|

1986

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tom 33

|

nr 5

8

Ribonucleic acids in the embryos of germinating wheat grains of different ripeness

63%

Weidner S., Kulka K.

Acta Societatis Botanicorum Poloniae

|

1980

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tom 49

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nr 4

9

Effect of osmotic stress on in vitro translational capacity of polysomes and on the composition of polysome-associated proteins in germinating seeds of pea (Pisum sativum L.)

63%

Brosowska-Arendt W., Weidner S.

Acta Societatis Botanicorum Poloniae

|

2012

|

tom 81

|

nr 3

Plant growth throughout the world is often limited by unfavourable environmental conditions. This paper reports results of a study on long- and short-term osmotic stress (−0.5 MPa) followed by a recovery on in vitro translational capacity of polysomes and on the composition of polysome-associated proteins in germinating pea (Pisum sativum L.) seeds. Here we show that, under osmotic stress, cytoskeleton-bound polysomes were charaterized by the highest translation activity, which may be indicative of an important role that this population of polysomes plays in the synthesis of the so-called “stress proteins”. We also find out that in response to osmotic stress, new proteins (22.01, 96.47 and 105.3 kDa), absent in the unstressed sample, associated with the total pool of polysomes, whereas the protein of 22.95 kDa, which was present in the embryonic tissue of seeds germinating under unstressed conditions, disappeared. These changes may have affected both the stability and the translational capacity of polysomes.

10

Ribonucleic acids of wheat grain and its endosperm during development and ripening

63%

Weidner S., Kulka K.

Acta Societatis Botanicorum Poloniae

|

1979

|

tom 48

|

nr 4

11

Importance of cytomatrix-bound polysomes to synthesis of lysine-containing proteins in triticale germs under ABA treatment

63%

Szypulska E., Weidner S.

Acta Physiologiae Plantarum

|

2011

|

tom 33

|

nr 4

The influence of exogenous abscisic acid (ABA) on the content of free polysomes (FP), membranebound polysomes (MBP), cytoskeleton-bound polysomes (CBP) and cytomatrix-bound polysomes (CMBP) in triticale germs as well as in vitro protein synthesis by these four polysomal fractions were studied. During translation, proteins were biotinylated for chemiluminescence detection. We have found that ABA changed both the content of FP, MBP, CMP and CMBP in germ tissue, and their subsequent translation activity. At 100 µM ABA, the content of FP and MBP was over fourfold lower compared to the control, whereas the amounts of CBP and CMBP were about two- and threefold higher, respectively. Moreover, the estimation of the share of polysomes in each ribosomal fraction (sub-units, monosomes, polysomes) showed that, at 100 µM ABA, cytomatrix-bound polysomes, which constituted 90% of polysomes, were the predominant class in ABA-treated germs while membrane-bound polysomes, which made up 82% of polysomes, dominated in the control. A high level of CMBP in ABA-treated tissues may indicate that this class of polysomes participates in ABAinduced synthesis of proteins. In turn, the inhibition of MBP under ABA-treatment is probably due to the delayed protein synthesis which takes place on these polysomes. We identified two lysine-containing proteins synthesized on both of the above classes of polysomes, whose synthesis was altered due to ABA application. Synthesis of a 47 kDa protein on MBP was inhibited, while synthesis of a 79 kDa protein on CMBP is strongly enhanced by ABA influence. The importance of these findings is discussed.

12

Effect of osmotic stress on the formation of a population of polysomes and their stability in pea (Pisum sativum L.) seeds

63%

Brosowska-Arendt W., Weidner S.

Acta Physiologiae Plantarum

|

2011

|

tom 33

|

nr 4

Plants growing under natural conditions are constantly exposed to various stress factors, which can restrain their productivity and limit yields. This paper deals with the effect of long- and short-term osmotic stress followed by recovery on the formation of polysomes and their stability during germination of pea (Pisum sativum L.) seeds. By isolating polysomes, it is possible to obtain an index which evidences the ability of tissues to synthesize proteins. Changes in the distribution of polysomes often precede measurable changes in amounts of proteins. Under osmotic stress, the dominant population of polysomes was the population of free polysomes (FP). The share of membrane-bound polysomes (MBP) and cytoskeletonbound polysomes (CBP) and cytoskeleton-membranebound polysomes (CMBP) in the total fraction of ribosomes increased under intensive (-1.0 and -1.5 MPa) osmotic stress. These results can suggest that the bound forms of polysomes play an important role in the synthesis of stress proteins. In addition, the stability of polysomes isolated from pea early seedlings growing under unstressed control and osmotic stress conditions was tested. It turned out that polysomes formed under osmotic stress conditions (especially the CMBP) were more resistant to the activity of exogenous ribonucleases than the polysomes in the control samples. Under stress conditions it is highly likely that ribosomes become more densely packed on mRNA thus making it more resistant to ribonuclease. This is just one of the many mechanisms regulating stability of mRNA.

13

Changes in ribosomal proteins of wheat embryos during accelerated ageing of the grain

63%

Zalewski K., Weidner S.

Acta Societatis Botanicorum Poloniae

|

1982

|

tom 51

|

nr 2

14

Frakcje kwasów rybonukleinowych oraz ich synteza w dojrzewających ziarniakach pszenicy

63%

Weidner S., Kulka K.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

1983

|

tom 258

15

ABA may affect the dormancy in triticale caryopses by inhibition of embryo class I RNases, enzymes involved in Pi release

63%

Szypulska E., Weidner S.

Acta Physiologiae Plantarum

|

2012

|

tom 34

|

nr 3

The main objective of this study was to analyze the differences in profiles of RNase activities from triticale embryos (Triticosecale, cv. Ugo) between dormant and non-dormant caryopses and to determine the influence of exogenous abscisic acid (ABA) on the activities of these enzymes. The major RNase from the examined tissue was detected following SDS-PAGE, with substrate-based gel assay, described by Yen and Green (Plant Physiol 97:1487–1493, 1991). The activities of enzymes were characterized according to their pH optima, ion dependence, EDTA sensitivity and DNase activity. In embryos with arrested growth (in a natural way by dormancy or artificially by ABA treatment), the activity of two enzymes—24 and 27 kDa—belonging to class I RNases was completely inhibited, whereas that of two other RNases of this family—23 and 25 kDa—was detectable. However, the activity of the class I ribonucleases (enzymes responsible for cellular Pi release) was very low. Moreover, in contrast with non-dormant caryopses, imbibing embryos of dormant or ABA-treated seeds contained 13- and 14-kDa enzymes. These enzymes have not been classified so far, and their specific properties are different from the generally accepted properties of ribonucleolytic enzymes. In addition to the above results, the Pi content in the analyzed samples was determined by the Ames (Methods Enzymol 8:115–118, 1966) method. The results suggest a very low and constant level of inorganic phosphate in dormant samples as well as an evidently decreasing Pi content in embryos under the influence of ABA treatment. The inhibition of the class I RNases activity induced by abscisic acid implies that one of the roles of ABA in seed dormancy may consist in arresting the catabolic release of Pi, which results in retarding the embryo’s growth.

16

The role of free, membrane-bound and tightly-bound polysomes in precocious germination of triticale caryopses

63%

Weidner S., Lukaszewicz D.

Acta Physiologiae Plantarum

|

1997

|

tom 19

|

nr 2

During the development and ripening of triticale caryopses cv. Grado, a gradual increase in the precocious germination ability of the grain was observed. After 48 hrs of incubation (at 22 °C under optimal moisture conditions for germination in darkness) of the freshly-collected caryopses of different ripeness, the percentage of polysomes in the ribosomal fraction isolated from embryonic tissue in buffer A + PTE (to yield released FP + MBP) was about 15 % in the sample of grains gathered at the milk-ripeness stage, over 27 % at the wax-ripeness stage and over 63 % at the full-ripeness stage. Percentages of polysomes solubilized in the pellets in buffer U (TBP, putative cytoskeleton-bound polysomes), were always higher and amounted to about 36 % at the milk-ripeness stage, 58 % at the wax-stage and 68 % at the full-ripeness stage. The incorporation of 3H-uridine into polysomal RNA (mRNA+rRNA) of triticale embryos increased during the development and maturation of the caryopses subjected to germination from about 19 and 33 Bq/mg RNA at the milk-ripeness in the FP+MBP and the TBP to 220 and 312 Bq·mg⁻¹ RNA at the full-ripeness stage, respectively. The effect of the pericarp and other tissues surrounding the embryo was also investigated. Removal of the outer pericarp strongly stimulated germination of unripe caryopses in the middle period of development e.g. 32 and 39 DAF. The strongest stimulatory effect on transcription was found in the embryo during germination, in all samples of caryopses of various degrees of ripeness, by isolating it completely from the pericarp, testa and endosperm. The high percentage of polysomes in the TBP and the high incorporation 3H-uridine into RNA in that fraction during precocious germination suggests an important role for this sub-population of polysome (and the proteins synthesised by them) in initiation of precocious germination processes.

17

Phenolic acids and dormancy in oat [Avena sativa L.] and rye [Secale cereale L.] caryopses

63%

Weidner S., Paprocka J.

Acta Physiologiae Plantarum

|

1996

|

tom 18

|

nr 4

18

Translational regulation of preharvest sprouting of Triticosecale caryopses

63%

Fraczek E., Weidner S.

Polish Journal of Natural Sciences. Supplement

|

2003

|

tom 01

19

Identification and properties of the ribonucleases from triticosecale embryos of dormant, non-dormant and ABA-treated caryopses

63%

Weidner S., Fraczek E.

Biological Letters

|

2005

|

tom 42

|

nr 2 Spec.Vol.

20

Possible involvement of the cytoskeleton in the regulation of barley caryopsis dormancy and germination

63%

Weidner S., Lukaszewicz D.

Acta Physiologiae Plantarum

|

1997

|

tom 19

|

nr 2

This study was conducted on barley cv. Ars. caryopses collected at full ripeness and divided into two batches. From one batch (dormant caryopses) polysomes were isolated from embryos immediately after harvesting and after two days of germination. From the other batch (non-dormant caryopses) the same was done after eight months storage in a dry state. A low ionic strength cytoskeleton-stabilizing buffer was used for the isolation of polysomes. Four different fractions of polysomes were examined: free polysomes (FP), membrane-bound polysomes (MBP), cytoskeleton-bound polysomes (CBP) and cytoskeleton-membrane-bound polysomes (CMBP). In germs grown from non-dormant caryopses, the first two fractions (FP + MBP) made up about 78 % of the total ribosomal material, whereas in embryos of dormant, imbibed caryopses, two last fractions (CBP + CMBP) made up about 71 %. The percentage of polysomes after 48 hours of imbibition of dormant caryopses in the FP, MBP and CBP was only about 13 % (i.e., 87 % monosomes), whereas a greater proportion (19.4 %) was found in the CMBP. The highest incorporation of ³H-uridine and ¹⁴C-amino acids (after 48 hours of germination and 0.5, 3 and 6 hrs incubation with precursors) took place in trhc CMBP both in dormant and non-dormant caryopses The major amount of the two polysome fractions associated with the cytoskeleton (CBP and CMBP) and the higher activity of CMBP in protein synthesis in embryos of dormant, imbibed triticale caryopses may indicate a significant role for polysomes associated with the cytoskeleton in the control of protein synthesis in dormant and germinating caryopses.

Ograniczanie wyników

Kwasy rybonukleinowe oraz fitohromy w rozwijających się ziarniakach zbóż

Role of gibberellins and cytokinins in regulation of germination during development and ripening of Triticale caryopses

Przedsprzętne porastanie ziarniaków zbóż i jego regulacja

II. Przedsprzętne porastanie ziarniaków zbóż i jego regulacja

The role of the embryoless parts of triticale caryopses in inhibiting precocious germination and transcription in the embryo during development and maturation of caryopses

Aktualne poglądy na kiełkowanie ziarniaków zbóż ze szczególnym uwzględnieniem biosyntezy kwasów nukleinowych

Mechanizm działania kwasu abscyzynowego

Ribonucleic acids in the embryos of germinating wheat grains of different ripeness

Effect of osmotic stress on in vitro translational capacity of polysomes and on the composition of polysome-associated proteins in germinating seeds of pea (Pisum sativum L.)

Ribonucleic acids of wheat grain and its endosperm during development and ripening

Importance of cytomatrix-bound polysomes to synthesis of lysine-containing proteins in triticale germs under ABA treatment

Effect of osmotic stress on the formation of a population of polysomes and their stability in pea (Pisum sativum L.) seeds

Changes in ribosomal proteins of wheat embryos during accelerated ageing of the grain

Frakcje kwasów rybonukleinowych oraz ich synteza w dojrzewających ziarniakach pszenicy

ABA may affect the dormancy in triticale caryopses by inhibition of embryo class I RNases, enzymes involved in Pi release

The role of free, membrane-bound and tightly-bound polysomes in precocious germination of triticale caryopses

Phenolic acids and dormancy in oat [Avena sativa L.] and rye [Secale cereale L.] caryopses

Translational regulation of preharvest sprouting of Triticosecale caryopses

Identification and properties of the ribonucleases from triticosecale embryos of dormant, non-dormant and ABA-treated caryopses

Possible involvement of the cytoskeleton in the regulation of barley caryopsis dormancy and germination