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This article describes a one-step root canal treatment and restoration of canine teeth in ferrets. Standard brass posts were used for the reconstruction of the teeth. The procedures were performed by dentists according to all rules and procedures used in root canal treatment in humans. The teeth were immediately restored anatomically. The procedures were successfully performed in four ferrets aged between 6 and 20 months of age, in three upper and one lower canines.
Amyotrophic lateral sclerosis is a multifactorial progressive neurodegenerative disorder leading to severe disability and death within 3-5 years after diagnosis. The main mechanisms underlying the disease progression are poorly known but according to the current knowledge, neuroinflammation is a key player in motor neurons damage. Astrocytes constitute an important cell population involved in neuroinflammatory reaction. Many studies confirmed their striking connection with motor neuron pathology and therefore they might be a target for the treatment of ALS. Cell-based therapy appears to be a promising strategy. Since direct replacement or restoring of motor neurons using various stem cells is challenging, enrichment of healthy donor-derived astrocytes appears to be a more realistic and beneficial approach. The effects of astrocytes have been examined using transplantation of glial-restricted precursors (GRPs) that represent one of the earliest precursors within the oligodendrocyte and astrocytic cell lineage. In this review, we focused on evidence-based data on astrocyte replacement transplantation therapy using GRPs in animal models of motor neuron diseases. The efficacy of GRPs engrafting is very encouraging. Furthermore, the lesson learned from application of lineage-restricted precursors in spinal cord injury (SCI) indicates that differentiation of GRPs into astrocytes before transplantation might be more advantageous in the context of axon regeneration. To sum up, the studies of glial-restricted precursors have made a step forward to ALS research and might bring breakthroughs to the field of ALS therapy in the future.
The aim of the study was to examine the changes in the density of VAChT (marker of acetylcholine present)-, NPY-, VIP-, SOM-, SP- and nNOS-immunoreactive (IR) nerve terminals and co-localization of VAChT with the above-mentioned neurotransmitters after the occurrence of dexamethasone (DXM)-induced ovarian cysts in gilts. DXM administration led to an increase in the density of VAChT/SP-, VAChT/nNOS- and NPY-IR nerve terminals around the cystic walls. In DXM-treated animals an elevated number of VAChT- and SP-IR nerve endings was found close to the tertiary follicles. Moreover, in the gilts receiving DXM the density of NPY-IR nerve endings (that simultaneously co-localized VAChT) was high near the interstitial gland. An increase in the number of VAChT/SP- and VIP-IR nerve fibers around the medullar arteries (A) was observed in cystic ovaries, while the number of VAChT-IR nerve endings near the cortical A was lowered after DXM application. Furthermore, nerve fibers containing VAChT were absent around veins in the whole ovary of DXM-treated animals. After DXM injections, an increase in the number of VAChT/SP- and VAChT/nNOS-IR nerve endings in the cortical, as well as VIP- and nNOS-IR (co-existing with VAChT), nerve terminals in the medullar part of the autonomic ground plexus (GP) was present. However, the administration of DXM led to a drop in the density of SOM-positive nerve endings (also VAChT-IR) in the medullar subdivision of the GP. The present study shows that in the porcine ovaries with DXM induced cysts the pattern of cholinergic innervation, as well as the co-localization of VAChT and NPY, VIP, SOM, SP or nNOS, were changed. Data obtained also suggest that acetylcholine and the above-mentioned neurotransmitters effecting the functioning (steroidogenic activity, blood flow) of the polycystic ovaries may have a significant influence on the course of this pathological status.
The aim of the study was to determine the existence and co-existence patterns of VIP and NPY in neurons and nerve fibers of porcine lumbar-sacral sympathetic chain ganglia. The studied ganglia were fixed with 4% buffered paraformaldehyde (perfusion) and then labeled by means of double-immunofluorescence using a mixture of antibodies cultivated in different species. The highest number of NPY-positive cells was observed in the lumbar ganglia and diminished in the direction of the caudal, where only single neurons were observed. In contrast, a different pattern of distribution was observed for VIP-positive neurons, whose number was higher in the more caudally located ganglia. Two populations of VIP-positive neurons could be distinguished: single, showing strong immunofluorescence and often with visible processes, located in the central part of the ganglia and a second population, composed of clusters of 4-8 cells and often co-localizing NPY. VIP-positive nerve fibers surrounded both NPY+ neurons and neurons lacking either NPY and/or VIP. The presence of a small number of NPY-positive neurons exhibiting very weak immunofluorescence in more caudally located SChG could suggest a "switch" of neuromediators produced there. An increase in the percentage of non-noradrenergic sympathetic neurons in more caudally located SChG may thus be implicative for a specific innervation pattern of target tissues of these ganglia.
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Health-supporting properties of beef

71%
Contemporary human diet contains excessive quantities of n-6 saturated fatty acids (SFA) and polyunsaturated fatty acids (PUFA), but it is deficient in n-3 fatty acids. This imbalance could be the cause of respiratory diseases, obesity and cancer. until recently, animal fat, including meat fat, was regarded solely as a source of saturated fatty acids, which are a risk factor for atherosclerosis, obesity and certain types of cancer. Recent studies have shown, however, that animal fats contain biologically active substances beneficial for health, and that only some saturated fatty acids have adverse consequences. The latter group includes lauric acid (C12), myristic acid (C14) and palmitic acid (C16), i.e. mainly the ones with an unfavorable n-6/n-3 fatty acid ratio. Apart from fat, beef also contains high amounts of minerals, mainly phosphorus, potassium, magnesium and zinc. Conjugated linoleic acid (CLA), in particular its cis-9 trans-11 and trans-9 cis-11 isomers, is one of the substances with health-supporting properties. Synthesized solely in the gastrointestinal tract of ruminants, CLA reaches the blood stream and is used by the mammary gland to synthesize milk fat. CLA is embedded in the animal's adipose tissue. There is a large body of research confirming CLA's ability to reduce the risk of atherosclerosis and obesity, and to lower cholesterol levels. when incorporated into the human diet in the amount of 1.5 to 3.5 g, CLA exerts anticarcinogenic effects (it inhibits the development ofbreast cancer, malignant melanoma, colorectal cancer and lung cancer). CLA prevents and alleviates the symptoms of type 2 diabetes; it is a powerful antioxidant and it boosts immunity. The highest quantities of CLA have been noted in the meat of ruminants. The CLA content of ruminant meat has been determined in the range of 10 to 33 mg 100 g- 1 of fat, being much higher than in pork (2-19 mg) and poultry (3.4 mg). Beef also has the most desirable n-6/n-3 fatty acid ratio at 6.3:1 in comparison with pork (12.7:1) and poultry (8.3:1). The highest levels of n-3 fatty acids can also be found in beef (5-6 g in 100 g fat). The objective of this study was to present and discuss the most recent findings concerning the fatty acid content of beef and its implications for human health. Source data were gathered and grouped thematically, and an attempt was made to characterize beef and its fatty acid profile.
Prosthodontic treatment, especially restorations of fractured teeth in small animals, has been the subject of many veterinary dental analyses in relation to techniques of endodontic treatment, preparation and cementation, as well as the general principles of prosthodontic treatment. The purpose of this paper is to present a previously undescribed method of all-in-one crown and root prosthetic restoration of fractured teeth in large dogs, together with a thorough analysis of the drawbacks, which may help veterinary dentists to use an evidence-based approach when deciding on the type of treatment for their patients with tooth fractures.
The aim of the study was to establish whether the dorsal root ganglion neurons supplying the porcine CaMG contain SP and/or CGRP and, additionally, which changes in the expression pattern of these peptides may be induced by a mechanical injury applied to the processes of the above neurons. The study was carried out on consecutive frozen serial sections of DRG taken from 12 eight-week old pigs, in which the neuronal tracer Fast Blue (FB) had been injected three weeks previously into the right CaMG. Six animals were then randomly chosen for ipsilateral ganglionectomy. Eventual changes in the chemical phenotypes of the injured cells were studied a week later using routine double-immunofluorescence labeling. FB+ neurons contained SP and CGRP (32% and 42%, respectively). The vast majority of SP- and CGRP-IR afferent cells belonged to the class of medium-sized (64% and 59%, respectively) and small neurons (32% and 37%, respectively). A co-localization of SP and CGRP was observed in 22% of FB+ neurons. The resection of CaMG resulted in a dramatic increase in the number of FB+ cells containing SP (55%) and a statistically significant decrease in the number of CGRP-IR neurons associated with CaMG (29%). These results suggest that sensory neurons associated with porcine CaMG contain SP and CGRP and that a re-section of CaMG is able to induce profound changes in the expression pattern of the studied peptides, implying deep mechanical injury-induced adaptative changes in the studied afferent neurons.
This article describes a procedure for a one-step root canal treatment and the subsequent restoration of fractured canine teeth in cats. Standard glass fibre posts were used in the reconstruction of the teeth which were then divided into two groups and restored anatomically using two different composite materials. The procedures were successfully performed on six cats. The subject teeth were four upper and two lower canines.
The aim of the study was to determine the relationship between the polymorphism of the ALA gene and the size of lymphocyte subpopulation expression of viral protein in cows naturally infected with bovine leukaemia virus. The studies were performed on a population of 97 Black-and-White breed cows, aged 3-8 years, from three large herds. The cows were examined three times in monthly intervals beginning from the second half of the first month after calving. Enzootic bovine leukosis diagnosis was based on the identification of p24 viral protein in the lymphocytes of the examined animals and on the white blood cell counts. The fluorescent antibody technique was used to detect p24 protein in the B (B-B2) and T CD4+ and CD8+ lymphocytes. The ALA gene polymorphism at the - 1689 position was assayed with the PCR-RFLP/Sdu I method. It was found that the ALA gene polymorphism differentiated the size of subpopulations of B-B2 lymphocytes and the T CD4+ lymphocytes expressing the p24 protein. This protein was more frequently registered in the B-B2 lymphocytes and in the T CD4+ and CD8 lymphocytes in cows with persistent lymphocytosis than in cows with the aleukaemic form.
The study was carried out on 10 male rabbits, New Zealand pure-bred, 3.5 months old, and weighing 2,000-2,200 g. The animals were divided into two equal groups: group 1 - subjected to short term lateral electrical surface stimulation (ST-LESS) for 2 h daily during 3 months, group 2 - control group without LESS stimulation but with other experimental parameters as in animals of the group 1 (SCOL-2 apparatus and preparation the skin to electrostimulation). Stimulation was performed with a Polish stimulator - SCOL-2, and clinical, macroscopic, and microscopic observations were performed. Similar growth of body mass was observed in the rabbits from both groups during the first two months of the experiment. In month 3 a slightly smaller increase in weight gain was observed in the animals from group 1 than in those from group 2. No morphological lesions were observed in a macroscopic examination. A statistically significant increase in the mean mass of the adrenal glands (0.4253 g +0.0033) of the stimulated rabbits in comparison to those from the group 2 (0.2981 g ±0.0087) was noted. A microscopic examination revealed hypertrophy of zona fascicularis with visible overgrowth of glandular cells in the rabbits from group 1. The results of clinical observations as well as morphological lesions indicate the presence of adaptive stress in rabbits stimulated with ST-LESS.
The aim of this study was to determine the presence of cocaine- and amphetamine-regulated transcript-like immunoreactive (CART-LI) neurons and co-localisation of CART with vesicular acetylcholine transporter (VAChT), neuronal nitric oxide synthase (n-NOS), vasoactive intestinal polypeptide (VIP), substance P (SP) and leu-enkephalin (LENK) in the enteric nervous system of the porcine gastric cardia by using a double-labelling immunofluorescence technique. CART-LI neurons were observed in the myenteric plexus (18.2}2.6%). A dense network of CART-LI nerve fibers was mainly observed in the muscular layer. CART showed co-localization mainly with VAChT, n-NOS, VIP and to a lesser degree with LENK and SP. Distribution of CART and its co- ocalization with other neurotransmitters suggest that this peptide plays an important role in gastric motility in the pig.
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