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The aim of the study was to determine the effect of exogenous sucrose and cytokinin on ethylene production and responsiveness in relation to the shoot formation of Pelargonium × hortorum 'Bergpalais' in vitro. Increasing the concentration of sucrose from 15 to 40 g L-1 in medium containing meta-topolin (mT) resulted in a two-fold decrease in the number of shoots and leaves as well as a reduction in ethylene production. The addition of ethylene synthesis inhibitor (AVG) to mT-medium significantly reduced the ethylene production and the shoot growth, but it had no significant influence on the shoot formation. The mT-induced shoot formation was, however, significantly reduced in the presence of ethylene action inhibitor (AgNO3), in a manner dependent on sucrose levels. At the end of the subculture period, increased sucrose concentrations (15–40 g L-1) in the presence of mT and AgNO3 resulted in a 3.7-fold increase in ethylene emission. At the same time, the supply of sucrose caused a 2.8-fold increase in the level of endogenous abscisic acid (ABA). Our results may suggest that the inhibitory effect of high sucrose concentration (30 and 40 g L-1) may depend on its influence on ethylene sensitivity. It also suggests that sucrose-regulation of the shoot formation of Pelargonium in vitro is mediated by ABA.
The effect of 3 levels soil compaction (low - 1.30, moderate - 1.47 and severe - 1.58 g·cm⁻³) on triticale and maize seedling shoots and root dry matter, leaf number, number and length of particular components of the root system, leaf injury (LI), leaf water potential (Ψ), chlorophyll content (Chl) and maximum quantum yield of PS II (Fv/Fm) were examined. For both examined species high level of soil compaction strongly affected the length of seminal and seminal adventitious roots and the number and length of nodal roots. Comparing to maize, a slightly weaker impact of soil impedance on the number and length of developed root system was observed in triticale. After 3 or 6 weeks of growth in the conditions of moderate and severe soil compaction a decrease in leaf number, dry matter of shoot and roots, and increase in leaf injury index and shoot to root (S/R) dry matter ratio were observed. Changes in root architecture under a high soil impedance were accompanied by changes in the leaf injury index (LI), leaf water potential (Ψ), chlorophyll content (Chl) and maximum quantum yield of PS II (Fv/Fm). On moderate or severe soil compaction level changes of the values of LI, Ψ, Chl and Fv/Fm were higher and statistical significant as comparing to a low soil compaction treatment. Similarly as in the case of root growth characteristic changes in physiological traits measured in leaves showed weaker impacts of high soil compaction for maize than triticale.
An influence of the soil drought on changes in leaf injury index (LI), leaf water potential (Ψ), chlorophyll content (Chl), chlorophyll a fluorescence (Fv/Fm) and a leaf fluorescence excitation spectrum in the main fluorescence bands (F450, F520, F690, F740) in maize and triticale was compared. In control treatments (C) among the examined species there occurred both differences and similarities in examined physiological traits. Also in the control plants differences between maize and triticale were observed in fluorescence measurements at wavelengths of 440 and 520 nm, however for wavelengths 690 and 740 nm the differences were insignificant. Drought stress (MD, SD) caused, in the case of triticale and maize genotypes, a significant decrease of leaf water potential, chlorophyll content and photochemical efficiency and increased leaf injury index. Those changes in, LI, Ψ, Chl and Fv/Fm values were higher in seedlings subjected to severe drought (SD) and harmful effect of drought in maize was higher than in triticale. Results of measurements of invisible leaf injury (LI) indicate the existence of a dependence of membranes injuries value from the length of the applied drought, leaf age and plant species. For both species injuries of older leaves were always higher comparing younger ones. Both for maize and triticale for each day of measurement in treatments MDR and SDR seedlings showed a tendency to slightly more efficient removal of harmful impact of drought on the leaf cell membranes injuries. Distinctly higher differences between triticale and maize were observed in the measurements of blue (F450) and green fluorescence (F520). Mean values of F450 and F520 in the case maize were of about 6 and 4 times respectively, higher than for triticale. However for red (F690) and far-red (F740) differences between triticale and maize there were no significance. Moderate and severe drought (MD, SD) caused the increase in emission and excitation of leaves in F450, F520 and F690 fluorescence range. After the recovery chlorophyll fluorescence in blue, green, red and far-red range mostly attained the obtained values of the control plants. Drought stress caused significant increase in F690/F740 ratio comparing to the control. After recovery changes in F450/520, F450/690 and F690/F740 ratio mostly did no attain the obtained values of control plants, however in most cases complete return to control plants was also observed.
In the present study, we evaluated the effects of exogenously applied 24- -epibrassinolide – one of the brassinosteroids – on the seed yield, seed chemical composition (content of soluble proteins, phytoestrogens, carbohydrates, minerals) and resistance to drought of soybean cultivated in Poland (cv. Aldana and Augusta). Quantitative and qualitative analysis of brassinosteroid content was made in seeds of plants treated with 24-epibrassinolide. Experiments were performed in the field and in greenhouse. Brassinosteroid was applied via presowing seed soaking (0,25 mg·dm-3) and spraying of plants (1 mg·dm-3). 24-epibrassinolide affected the increase of the weight of seeds per one plant (27-73%) depending on the cultivar as well as growth conditions (occurrence or lack of drought factor at the stage of seed setting). In the seeds, 24- -epibrassinolide did not change content of proteins or soluble carbohydrates, but increased phytoestrogen genistein content in cultivar Aldana and decreased level of potassium in cultivar Augusta and calcium level in cultivar Aldana. In soybean seeds, the presence of brassinolide and castasterone was found. 24-epibrassinolide applied exogenously to plants was not accumulated in newly formed seeds. In drought-stressed soybean brassinosteroid showed protective effect on net photosynthesis and photosystem II efficiency.
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