Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 20

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Fate of glial progenitors is dictated by the local tissue microenvironment

100%
Sypecka J., Sarnowska A.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr 1
The oligodendrocyte progenitors (OPCs) are the abundant population of NG2-positive cells in the young and adult CNS. They are capable of myelinogenesis, but they are also among the first cells to react to CNS injuries. Over the last decade, these glia commited progenitors have been however the subject of intensive research in context of their assumed neural stem cell properties. In our studies we have addressed the question of the impact of the local tissue microenvironment on the OPC commitment and differentiation. Their susceptibility to external stimuli and assumed intrinsic neurogenic potential have been investigated in co-culture models with organotypic slices derived from two distinct CNS regions (hippocampus and spinal cord). The hippocampal slice culture exposed to oxygen glucose deprivation (OGD) was used to evaluate the cell differentiation in microenvironment conditioned by traumatized tissue. The results have shown that the local instructive clues not only trigger the neuronal commitment of oligodendrocyte progenitors, but also govern the oligodendroglial maturation. While the trophic factors secreted by hippocampal slices efficiently promoted neurogenesis, the observed effect was significantly abolished in co-cultures with the OGD-subjected tissue. The less pronounced susceptibility to adopting neuronal phenotype and the considerable slowdown of oligodendroglial differentiation was observed in the co-cultures with the spinal cord slices. Our findings indicate that OPCs actually meet some of the neural stem cell criteria. The obtained results also suggest that the specificity of the instructive clue cocktail might module the fate choice of mobilized endogenous or transplanted cells, which is important while planning neurorepair strategies. Supported by grant 0345/B/P01/2010/38.
2

The neuroprotective efFect exerted by oligodendroglial progenitors on ischemically impaired hippocampal slices

100%
Sypecka J., Sarnowska A.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
The role of oligodendrocytes is supposed to go beyond the process of myelinating nerve fibers in the central nervous system.  Pre-clinical studies based on oligodendrocyte progenitor cell (OPCs) therapies revealed a significant behavioral improvement in spite of failure in the remyelination process in animal models of demyelinating diseases. To address the issue of potential trophic support conferred by OPC to diseased nervous tissue, co-culture experiments with neonatal rat OPCs and organotypic hippocampal slices were designed. For this purpose, rat organotypic hippocampal slices were exposed to a brief oxygen and glucose deprivation (OGD) which allowed mimicking an ischemic injury in vitro. Soon after the OGD procedure, the hippocampal slices were co-cultured together with differentiating oligodendroglial progenitors. Cell survival and their proliferation rates in the injured slices were estimated by immunohistochemical methods. After acknowledging the beneficial influence of the neighboring oligodendrocytes on the injured tissue, a set of molecular and biochemical experiments were carried out with the aim of determining the mechanism(s) of the observed profound neuroprotective effect. The results of both molecular studies and functional assays revealed that the oligodendrocyte-derived BDNF is the major factor promoting neuronal survival in OGD-subjected hippocampal slices, while SCF and IL-10 strongly promote the cell proliferation. Among the newly-born cells, neuroblasts and microglia could most frequently be found pointing to both the neuroprotective and neuroimmunomodulatory role of differentiating oligodendrocytes. In conclusion, the presented study revealed that oligodendrocytes are able to secret BDNF and other active factors, thus providing trophic support for other neural cells. The presented study confirms the hypothesis concerning the complex functions of oligodendrocytes in the nervous tissue. Supported by MMRC statutory funds.
3

The crucial role of the local microenvironment in fate-decision of neonatal rat NG2 progenitors

81%
Sypecka J., Sarnowska A., Winiarska K., Domanska-Janik K.
Acta Neurobiologiae Experimentalis
|
2009
|
tom 69
|
nr 1
The fate choice of neural progenitors could be dictated by local cellular environment of adult CNS. The aim of our study was to investigate the effect of hippocampal tissue on the differentiation and maturation of the oligodendrocyte NG2 precursors. Methods: The hippocampal slice culture was established from the brains of 7-day old rats. The NG2 precursors, obtained from a 12-day old mixed primary culture of neonatal rat hemispheres, were labeled with CMFDA and seeded on hippocampal slices. After 7ñ14 days in co-culture, the cells were stained with neural markers. Results: The NG2 cells differentiated predominantly into oligodendrocytes, presenting various stages of maturation: progenitors NG2+, O4+ and fi nally mature Galc-positive cells. However, except for a few cells with astrocyte-specifi c S100β staining, a considerable number of these cells differentiated into TUJ+ and MAP-2+ neurons. Moreover, a certain population of these cells preserved proliferative properties of primary precursors, as revealed by the Ki67 expression. Conclusions: Neuronal microenvironment provided by the culture of hippocampal slices is potent to induce neurogenesis from oligodendrocyte NG2+/PDGFRα+/CNP+ progenitors and promotes their differentiation not only into macroglia but also into neurons. It also sustains their proliferative capacity. The results indicate a crucial role of the local cellular environment in fatedecision of primary NG2+ multipotent neural progenitors, which may affect their behavior after transplantation into CNS. Supported by MSHE grant N40101832 /0296
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Technologie XXI wieku i komórki macierzyste w badaniach i terapii schorzeń neurologicznych

81%
Buzanska L., Zychowicz A., Sarnowska A.
Wszechświat
|
2015
|
tom 116
|
nr 01-03
5

Transplantation of neurospheres and organoids derived from human umbilical cord blood onto hippocampal organotypic slice culture

81%
Sarnowska A., Jurga M., Domanska-Janik K.
Acta Neurobiologiae Experimentalis
|
2006
|
tom 66
|
nr 4
6

Neural commitment of cord blood stem cells (HUCB-NSC/NP): Therapeutic perspectives

81%
Domanska-Janik K., Habich A., Sarnowska A., Janowski M.
Acta Neurobiologiae Experimentalis
|
2006
|
tom 66
|
nr 4
Human umbilical cord blood (HUCB) is considered a promising source of neural progenitors capable of being used for cellular therapies in neurological disorders. Here we review briefly our work on the elucidation of mechanisms and development of practical standards as regards the selection, maintenance and use of cord blood derivatives for such purposes. Our results join those of other recent studies in suggesting strongly that, the generation of neural-like cells from tissue belonging to a different germ layer (such as a cord blood is) is most probably explained by reference to a discrete subpopulation of embryonic-like stem cells of pluripotent characteristics. Such cells identified in cord blood through their expression of specific genetic and protein markers can be expanded in vitro and directed toward neurally-committed progenitors differentiating further into more mature neuron-like or macroglia-like cell phenotypes. From this HUCB-derived neural progenitor fraction a novel neural-like stem cell line (HUCB-NSC) has been developed, and characterized in respect of in vitro and in vivo (posttransplantation) properties.
7

Diazepam and cyclosporin A:the promising neuroprotectants

81%
Sarnowska A., Beresewicz M., Zablocka B., Domanska-Janik K.
Acta Neurobiologiae Experimentalis
|
2006
|
tom 66
|
nr 4
8

Hippocampal microenvironment instructs NG2 precursors to become neurons

81%
Sypecka J., Sarnowska A., Domanska-Janik K.
Acta Neurobiologiae Experimentalis
|
2007
|
tom 67
|
nr 3
9

Human mesenchymal stem cells in the treatment of neurological diseases

81%
Drela K., Siedlecka A., Sarnowska A., Domanska-Janik K.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr 1
Here we provide a comprehensive data on the unique features of mesenchymal stem cells (MSCs) which makes them feasible and preferred candidate for cell-based therapy in neurological clinic. From this point of view the most important features of these cells are: (1) availability from autologous sources independently from age of patient; (2) extensive expansion in vitro; (3) immunomodulatory "bystander" function after transplantation in vivo; (4) potentiality to protect, repair or eventually replace impaired or dysfunctional host cells. For complete these last task of functional regeneration of central nervous system, we have to take advantages of MSCs capability for transient, time-locked proliferation, migration to site of injury and their commitment to neuronal differentiation. However, if we are to make progress in the use of MSCs for therapy in the clinic it will be necessary to establish more unified, advanced standards for cells processing in vitro as well as safer and improved procedures for their delivery in vivo.
10

Involvement of MMPs in delayed neuronal death after global ischemia

81%
Zalewska T., Ziemka-Nalecz M., Sarnowska A., Domanska-Janik K.
Acta Neurobiologiae Experimentalis
|
2002
|
tom 62
|
nr 2
11

Heterogeneity of the local tissue microenvironment influences differentiation of NG2 cells

71%
Sypecka J., Sarnowska A., Szablowska-Gadomska I., Lukomska D., Domanska-Janik K.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr 1
The NG2 cells are the oligodendrocyte precursors that terminally differentiated are capable for myelination of central nervous system (CNS). They exhibit many features of neural stem cells and constitute the abundant population of dividing progenitors in the young and adult brain. A question arises if their commitment could be modulated by local tissue-specific or neuropathological signals. The aim of our study therefore was to evaluate the effect of distinct microenvironments (provided by either the spinal cord or the hippocampal slices) on the differentiation of neonatal NG2 cells. Subsequently, hippocampal slice culture subjected to an ischemic injury (the glucose-oxygen deprivation, OGD) was used in order to evaluate the cell development in microenvironment conditioned by traumatized tissue. Methods. Both the hippocampal and spinal cord slice cultures were established from the same 7-day old rats. The model of an indirect contact (i.e. exclusively by the culture media) in co-culture system was chosen to eliminate the influence of cell-cell contact. The NG2 cells were obtained from 10-day old mixed primary culture of neonatal rat hemispheres. After 7 days in co-culture, the cells were either stained with neural markers or collected for the RNA isolation and real-time PCR. Results. The medium conditioned by hippocampal slices effectively promoted neurogenesis: ~30 % of NG2 cells differentiated into TUJ 1-positive neurons. The remaining fraction mostly formed premyelinating and mature oligodendrocytes. The exposition of hippocampal slices to the OGD injury abolished the effect of pro-neuronal induction in co-cultures. In media conditioned by spinal cord slices, neurogenesis was less pronounced (20% neurons) and the oligodendrocyte differentiation was significantly slowed-down. Conclusions. The NG2 cells were shown to have intrinsic potency for neurogenesis. Heterogeneity of local microenvironment might modify the fate of endogenous or transplanted NG2 cells what should be taken into consideration in potential neurorepair strategies. Supported by grant 0345/B/P01/2010/38.
12

The impact of local tissue microenvironment on the oligodendrocyte progenitor differentiation

71%
Sypecka J., Sarnowska A., Winiarska H., Domanska-Janik K., Lukomska B.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
The oligodendrocyte precursors exhibit many features of neural stem cells and constitute the abundant population of dividing progenitors in the young and adult brain. A question arises if their commitment and development could be modulated by either local tissue-specific or neuropathological signals. The aim of our study was to evaluate the effect of distinct microenvironments (provided by either the spinal cord or the hippocampal slices) on the differentiation of rat neonatal NG2 cells. The hippocampal slice cultures subjected to an ischemic injury (OGD) were used to mimic the traumatized tissue microenvironment. Both the hippocampal and spinal cord slice cultures were established from the same 7-day old rats. The model of an indirect contact (i.e. exclusively by the culture media) in co-culture system was chosen to eliminate the influence of cell-cell contact. The NG2 cells were obtained from 10-day old mixed primary culture of neonatal rat hemispheres. After 7 days in co-culture, the cells were either stained with neural markers or collected for the RNA isolation and real-time PCR. The medium conditioned by hippocampal slices effectively promoted neurogenesis: ~30% of NG2 cells differentiated into TUJ 1-positive neurons. The remaining fraction mostly formed premyelinating and mature oligodendrocytes. The exposition of hippocampal slices to the OGD injury abolished the effect of pro-neuronal induction in cocultures. In media conditioned by spinal cord slices, neurogenesis was less pronounced (20% neurons) and the oligodendrocyte differentiation was significantly slowed-down. The NG2 precursors have the intrinsic potency for neurogenesis. Heterogeneity of local microenviroment might modify the fate of endogenous or transplanted NG2 cells what should be taken into consideration in potential neurorepair strategies. Supported by grant 0345/B/P01/2010/38.
13

Lowered oxygen concentration and culture in 3-dimension structure enhance immunological response of WJ-MSC to proinflammatory factors

71%
Figiel-Dabrowska A., Lech W., Zychowicz M., Domanska-Janik K., Sarnowska A.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: MSC-based therapy is becoming more and more common treatment of various diseases, albeit still as an experimental approach. According to present‑day literature, the therapeutic effects of transplanted cells would not be ascribed to their differentiation, trans‑differentiation or repopulation but rather to their paracrine effect on damaged tissue. This way of treatment can be initiated and enhanced by local environmental mediators. AIM(S): The aim of this study was to assess the influence of inflammation specific environment in vitro on secretory WJ-MSC properties and to evaluate the possibility of programmed and controlled induction and enhancement of anti‑inflammatory cell properties in the context of further cell therapy. METHOD(S): Our experiments were based on reconstruction in vitro the environment to which therapeutic cells (WJ‑MSC) are usually transplanted. The inflammatory conditions that occur around the transplant were reproduced through TNFα and IFNγ stimulation. Tissue specific oxygen concentration (5%), 3‑dimension transplant structure and chemical composition of the indirect transplanted cell surrounding as determined by additional scaffold ingredients (fibrin and platelet lysate) were also reconstituted. RESULTS: Carried experiments have shown specific changes in the secreted cytokine pallet induced in vitro by the inflammation‑like WJ‑MSC surrounding. We have proved that environmental modifications cause changes in synthesis and secretion of the determined proteins. Both, the physioxia introduced in our in vitro experiments and WJ cells cultured in 3-dimensional structures enhanced cytoprotective paracrine properties of WJ-MSC. Additional reinforcing effect was observed when therapeutic cells were transplanted on platelet lysate – containing scaffolds. CONCLUSIONS: Presented results indicate that by optimization of cell culture and transplantation conditions we could control and enhance cytokine-connected therapeutic properties of MSC. FINANCIAL SUPPORT: The work was supported by National Centre for Research and Development grant No Strategmed 1/234261/2/NCBR/2014.
14

Differentiation of glia-committed NG2 cells: The role of factors released from hippocampus and spinal cord

71%
Sypecka J., Sarnowska A., Gadomska-Szablowska I., Lukomska B., Domanska-Janik K.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr 1
The NG2-positive cells are the oligodendrocyte precursors, which, when terminally differentiated, are capable of myelinating the central nervous system. There is however an ever-growing list of evidences that NG2 cells actually possess an intrinsic neurogenic potential and they are capable of neuronal differentiation in response to environmental stimuli. To address the question, we have established a model of an indirect co-culture system of the freshly isolated rat neonatal NG2 cells and organotypic slices derived from two distinct CNS regions (hippocampus and spinal cord) to mimic the nervous tissue microenviroment. The cell differentiation in microenvironment of OGD-injured hippocampal slices has been studied as well. The molecular analysis of selected trophic factors has been performed to determine the patterns of their expression. Indeed, the comparison of the cell commitment and development in various microenvironments has pointed to significant dissimilarities. First of all, the medium being continuously conditioned by the hippocampal slices efficiently promoted neurogenesis. The effect has been significantly abolished in co-cultures with the injured tissue. The less pronounced susceptibility to adopting neuronal phenotype and the considerable slowdown of oligodendroglial development was observed in the co-cultures with the spinal cord slices. The role of BDNF in oligodendroglial progenitor commitment and development has been investigated proving that it is one of the key players in the examined processes. The specificity of the instructive clues cocktail might module the fate choice of mobilized endogenous or transplanted cells, which should be taken into consideration while planning neurorepair strategies.
15

Striking differences between mesenchymal stem cells derived from neonatal (Wharton jelly) and adult (bone marrow) human tissues

61%
Drela K., Siedlecka P., Wielgos M., Sarnowska A., Lukomska B., Domanska-Janik K.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr 1
Mesenchymal stem cells (MSC) exert unique ability to differentiate into various cells of mesodermal origin. These properties place MSC as a very promising source of cells for regenerative medicine and tissue engineering. Recently, it has been shown that experimental transplantation of MSC improves a variety of neurological dysfunctions. Bone marrow (BM) represents the mostly exploited source of human therapeutic stem cells but similar populations have been recently identified in many other tissues and organs. Among them umbilical cord Wharton jelly (WJ) has been recognized for its safety, accessibility and differentiation potential. This study compares human Wharton jelly-derived MSC (WJ-MSC) and human bone marrow-derived MSC (BM-MSC) in terms of cell phenotype, optimal growth and multilineage differentiation characteristics with special attention to neurogenic potential demonstrated by both type of cells. Materials and Methods: MSC were isolated from human Wharton jelly and human bone marrow then cultured in vitro in Lonza medium in defined conditions. Then both cell types were subjected to the specific induction media (Gibco) to analyze their potential to differentiate into osteo-, chondro- adipo- and myogenic lineages. Transcriptional activity of genes characteristic for early and late stages of cell differentiation has been examined using RT-PCR. Concomitantly immunochemical analysis of certain gene-related proteins has been performed by immunocytochemical methods. Results: We have demonstrated that both isolated WJ-MSC and BM-MSC exhibited characteristic, mesenchymal cell specific phenotypes by expressing the panels of surface antigens (CD73, CD90, CD105, CD166) as well as typical for MSC multilineage differentiation markers. However, efficiency of these processes differs markedly between the cells derived from each of examined tissues. Thus, WJ-MSC appeared to be much less prone to adipogenic differentiation in comparison to BM-MSC. In contrast, WJMSC revealed higher proliferation and neural differentiations potential than BM-MSC. Consistently, only WJ-MSC-derived cells unveiled neural progenitor characteristics expressing panel of cellular markers typical for neural lineage differentiation, i.e. Nestin, NF200, GFAP. All together, these data allow us to hypothesize that the fetal origin of WJ tissue determines its distinguished neuro-mesenchymal characteristic. This is consisted with the data of Takashima et al. (2007) showing that neonatal MSC cultures contain substantially high number of cells being descendants of the earliest wave of developmentally discern neuroepithelial MSC lineage derived from cranial part of neural crest and clearly partitioned from MSC residing in adult bon marrow niche. Conclusions: The study demonstrated that WJ-MSC, similarly to BM-MSC, can be effectively expanded in culture up to 6–8 passages when maintaining cells in undifferentiated state expressing common MSC markers. In contrast, the both MSC lines differ markedly in their ability to lineage differentiation. The most striking difference was that only WJ-MSC can be induced to neural phenotypes. Consisted with this observation WJMSC seems to be more favorable than BM-MSC to cell replacement therapy of neurodegenerative diseases. Supported by NSC grant No 2011/01/B/NZ3/0540
16

Serum free culture of 3D scaffold-based aggregates of human neural stem cells derived from cord blood

51%
Jurga M., Sarnowska A., Habich A., Janowski M., Lukomska B., Lipkowski A. W., Kurzepa K., Domanska-Janik K.
Acta Neurobiologiae Experimentalis
|
2006
|
tom 66
|
nr 4
17

Human umbilical cord wharton jelly grafts: preliminary characterization and effect of transplantation in a rodent model of stroke

51%
Sarnowska A., Jablonska A., Jurga M., Strojek L., Lukomska B., Forraz N., McGuckin C., Domanska-Janik K.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr 1
Over the last decade a large number of studies explored the use of cord-blood-derived stem cells for treatment of neurological disorders. Despite of some positive preclinical results low survival of transplanted cells was noticed in the host brain. It seems that transplantation of the donor cells in their own milieu might be more effective due to the natural cell-cell contact and the presence of growth factors and cytokines. The concept of our studies was to deliver human stem cells resided in Wharton jelly (WJ) of umbilical cord tissue into rat brain. 3D tissue implant contains mesenchymal stem cells (MSCs) capable of multilineage differentiation. In the current study we performed in situ analysis of entire WJ-MSCs after their transplantation into: hippocampal organotypic slices isolated from neonatal rats (i); the striatum of normal (ii) or focally injured (iii) adult Wistar rats. Results: Seven days after grafting on hippocampal slices intense migration of the donor cells (NuMa+) from 3D WJ implant was noticed. Double-labeling showed co-localization of NuMa marker with NF200 or GFAP activity in these cells whereas donor cells residing in 3D WJ implant did not express any neural markers. Interestingly, in intact brain all WJ-MSCs (NuMa+) cells remained in 3D tissue implant but differentiated into NF200+ and GFAP+ phenotypes during the time of observation. In contrast, after WJ implantation into injured rat brain the donor MSCs proclaimed migration towards ischemic boundary regions. Moreover, NF200 or GFAP markers were localized among both, migrating as well as remaining in the graft NuMa+ cells. Conclusions: Transplantation of WJ-MSCs in 3D tissue implant into adult rat brain improves cells survival within graft and induces their spontaneous transition into cell of neural lineage. Brain injury additionally stimulates migration of these donor cells out of WJ implant with their further differentiation in the host tissue. Supported by Fondation Jerome Lejeune grant
18

Neurogenic potential of human mesenchymal stem cells transplanted inside 3D gelatin/laminin scaffolds or wharton jelly implants into adult rat brain

42%
Jablonska A., Drela K., Sarnowska A., Wanacka E., Jurga M., Dainiak M., Jungvid H., Forraz N., McGuckin C., Domanska-Janik K., Lukomska B.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr S
Mesenchymal stem cell (MSC) transplantation offers new therapeutic avenue for neurological diseases, however limitted survival of exogenous cells in the host brain is a major setback. The aim of this study was to evaluate the efficacy of using biodegradable scaffolds or Wharton jelly implants contained MSC after their transplantation into rat brain.Materials and methods: Adult Wistar rats were transplanted with MSC derived from human umbilical cord (hUC-MSC) (i), hUC-MSC localized inside biodegradable gelatin/laminin scaffolds (hUC-MSC/GL) (ii) or Wharton jelly implants (hUC-MSC/WJ) (iii). Results: hUC-MSC cultured in vitro expressed CD73, CD90, CD166 and Oct3/4, Nanog1, Nestin markers. 7 days after hUC-MSC transplantation only few viable donor cells were observed in the rat brain surrounded by heavy infiltration of macrophages/microglia (ED1+) and activating astrocytes (GFAP+). Hence transplantation of hUC-MSC/GL resulted with better cell survival compared with hUCMSC grafted in suspension. There was no donor cell migration out of the scaffolds however hUC-MSC lodged inside G/L scaffolds attained early neural markers. The inflammatory cell influx observed around the scaffolds was less intense with few ED1+ cells present in the core of scaffold. Similarly, all transplanted hUC-MSC/WJ remained in 3D tissue implants. Some of these cells adopted NF200, A2B5 or GFAP phenotypes during 7 days of observation. Concomitantly only scarce infiltration of immunoreactive cells was seen. Conclusions: Transplantation of hUC-MSC in 3D G/L scaffolds or hUC-MSC/WJ implants into adult rat brain improves survival of donor cells and induces their spontaneous transition into cell of neural lineage. It seems that 3D structures protect cells localized inside them from the host immune cells and may allow the diffusion of nutrients and other factors to propagate cell survival and differentiation into neuronal lineage. Supported by MSHE grant no N401 014235 and Fondation Jerome Lejeune grant.
19

Preclinical characteristics and regenerative potential of adipose – derived MSC

42%
Sarnowska A., Figiel-Dabrowska A., Szczepanik E., Kuzma M., Mierzewska H., Antczak-Marach D., Kruk M., Terczynska I., Krzesniak N., Sawicka E., Nowak A., Noszczyk B., Kaminska A., Marchel A., Domanska-Janik K.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
While the rapid development of stem cell-based therapies are nowadays running, the reliable protocols leading to safe and effective way for cell isolation, expansion and commitment in vitro according to distinguished therapeutic purposes still need more consensus and standardization. In order to obtain particular disease-committed therapeutic cells we have screened various culture conditions, especially new systems involving lowered oxygen tension and small molecule treatments which may “rejuvenates” MSC. We also looked on the changes in grow dynamics of long-term cultures in various oxygen concentrations to dissect changes that predict genetic instability of cultivated cells. The standardization of such type of culture (by additional criteria beyond the framework developed by ISCT) should further enhance life-span, expansion and differentiation potential of MSCs needed for more effective regeneration of diseased brain. In recent years we have also entered into the clinic with individual medical experiments (in accordance with the guidelines described by A. Korczyn’ Sieratzki – Chair of Neurology Tel Aviv University 2010) based on mesenchymal regenerative cell therapy to elaborate save therapeutic procedures for autoimmune epilepsy, ALS and vast nerve injury. First results are promising and indicate regenerative and immunomodulatory properties of transplanted MSC. FINANCIAL SUPPORT: The work was supported by National Centre for Research and Development grant No STRATEGMED 1/234261/2/NCBR/2014.
20

MRI-monitored cord blood-derived cell transplantation to the ventricular system of child with global cerebral ischemia-a case report

42%
Janowski M., Kmiec T., Jurkiewicz E., Kropiwnicki T., Habich A., Kotulska K., Jelonek E., Sarnowska A., Litwin M., Boruczkowski D., Lukomska B., Walecki J., Roszkowski M., Jozwiak S., Domanska-Janik K.
Acta Neurobiologiae Experimentalis
|
2011
|
tom 71
|
nr 1
Introduction: Neurological disorders are the most common cause of serious disability and have a major impact on financial healthrelated burden to society. Most of them are definitely associated with cell death: sudden or chronic. Conventional treatment methods yield disappointing results. Thus the discoveries in stem cell biology have fueled the interest in cell-based therapeutical approach. Based on experimental data cord blood has been proposed as a novel, autologous cell source for pediatric population. Non-invasive monitoring of cell fate following transplantation has been recently recommended as a basis for rational stem cell therapy. Subject: One year old child experienced devastating, cardiac arrest-induced cerebral ischemia. Despite a broad rehabilitation program diagnose of vegetative state has been established three months later. After next three months of continued rehabilitation no noticeable improvement has also been found and the child has been included into study. The protocol has been approved by the ethical commission of The Children’s Memorial Health Institute in Warsaw, Poland. Then the child’s own cord blood cells have been neurally-converted over 10 days in culture within GMP facility. Prior to transplantation cells were labeled with iron oxide (SPIO) for MR imaging. For scaling sensitivity of MR signal different concentrations of SPIO-labeled cells were scanned in the phantom. Then patient received monthly 3 subsequent cell infusions (1.2 x 107 cells each) to lateral ventricles. The follow up continued up to 6 months and included both clinical assessment and MR examinations. Results: High efficiency of neural cell conversion and SPIO labeling as well as no cytotoxicity were observed. The employed method of cell transplantation was found to be efficient to deliver cells to CNS as confirmed by MR imaging. Gradual decrease of SPIO signal intensity was observed over the period of follow up. No adverse events or abnormal reaction to cell implantation was detected. The follow up revealed mild functional improvement - decreased nystagmus, spasticity and the number of epileptic seizures. Moreover, the features of the child contact with parents has appeared, thus vegetative state can not be diagnosed any more. Conclusions: This report indicates that transplantation of autologous, neurally-committed cord blood-derived cells to the ventricular system of child is safe, feasible and able to result with mild functional improvement. Additionally cell-related MRI signal can be monitored for more than 4 months in transplanted brain hemisphere. Supported by MSHE grants no 0141/B/P01/2008/35 and 0142/B/ P01/2008/35.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.