Wyniki wyszukiwania

1

Zastosowanie systemu ARAMIS do pomiarów odkształceń konstrukcji murowych

100%

Urbanska K.

Zeszyty Naukowe Uniwersytetu Zielonogórskiego. Inżynieria Środowiska

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2017

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nr 46[166]

2

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Modele doświadczalne w badaniach onkologicznych. Część I. Sferoidy i model in ovo

63%

Urbanska K., Sokolowska J.

Życie Weterynaryjne

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2012

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tom 87

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nr 10

3

Komórki C tarczycy w warunkach fizjologicznych i stanach patologicznych. Część II. Rozrost komórek C i rak rdzeniasty

63%

Sokolowska J., Urbanska K.

Życie Weterynaryjne

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2018

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tom 93

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nr 08

4

Immunohistochemical assessment of metalloproteinases MMP2 and MMP9 expression in canine various subtypes of lymphomas in relation with proliferative and apoptotic markers

63%

Sokolowska J., Urbanska K.

Polish Journal of Veterinary Sciences

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2019

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tom 22

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nr 2

5

Survivin expression in correlation with apoptotic activity in canine lymphomas

63%

Sokolowska J., Urbanska K.

Polish Journal of Veterinary Sciences

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2017

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tom 20

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nr 2

Survivin regulates cell cycle and mitosis and has antiapoptotic properties. Because of its dual function survivin has been the subject of much research focusing on its role in tumorigenesis and the relationship between survivin expression and apoptotic and/or proliferative activity in many types of human tumor including non-Hodgkin’s Lymphomas. Such studies have not been conducted in canine lymphomas. The aim of this study was to evaluate the expression of survivin in canine lymphomas of low (5/25) and high (20/25) grades in relation to apoptotic markers (apoptotic index and index of caspase-3). Survivin was found in all examined lymphomas. Most tumors (18/25) showed survivin expression in 10%-25% of positive cells. Only in single cases was lower (0-10% positive cells, 1/25) or higher (25%-50% and >50% positive cells, 5/25 and 1/25, respectively) survivin expression. No significant differences between mean values of either index of survivin or apoptotic index was found between low and high grade lymphomas. However, such a difference among lymphoma grades was shown regarding the caspase-3 index. No correlation between the survivin index and either the apoptotic index or caspase-3 index was found, irrespective of the method of quantification: in whole specimens or in areas of low and high survivin expression. Positive correlation was consistently noted only between both apoptotic markers. The results indicate that survivin is commonly expressed in canine lymphomas. It seems that survivin does not exhibit anti-apoptotic activity in canine lymphomas. Lack of correlation between survivin expression and apoptotic markers could indicate its potential role in cell cycle activation in lymphoma cells.

6

Kwas dokozaheksaenowy [DHA] - kluczowy skladnik tkanki nerwowej

63%

Mirowski A., Urbanska K.

Magazyn Weterynaryjny

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2010

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tom 19

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nr 02

178-179

7

Modele doświadczalne wykorzystywane w badaniach onkologicznych. Część II. Modele zwierzęce

63%

Urbanska K., Sokolowska J.

Życie Weterynaryjne

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2012

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tom 87

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nr 11

8

Komórki C tarczycy w warunkach fizjologicznych i stanach patologicznych. Część I. Różnicowanie, morfologia, lokalizacja i funkcje komórek C

63%

Sokolowska J., Urbanska K.

Życie Weterynaryjne

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2018

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tom 93

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nr 07

9

Biologiczna rola surwiwiny i jej znaczenie kliniczne

63%

Sokolowska J., Urbanska K.

Życie Weterynaryjne

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2011

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tom 86

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nr 12

10

Wpływ kwasu dokozaheksaenowego na ośrodkowy układ nerwowy

63%

Mirowski A., Urbanska K.

Życie Weterynaryjne

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2010

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tom 85

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nr 05

11

Wpływ pokrycia dachu na ilość i wielkość zawiesin w spływach deszczowych

51%

Burszta-Adamiak E., Urbanska K., Draganski P.

Przegląd Naukowy. Inżynieria i Kształtowanie Środowiska

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2017

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tom 26

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nr 4[78]

12

Rola glikoproteiny P w warunkach fizjologicznych i stanach patologicznych. Część II. Znaczenie glikoproteiny P w patogenezie niektórych chorób i rozwoju oporności wielolekowej

51%

Sokolowska J., Urbanska K., Klosinska D.

Życie Weterynaryjne

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2014

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tom 89

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nr 12

13

Zmienność parametrów wydzieliny dróg rodnych w przebiegu cyklu rujowego bydła ze szczególnym uwzględnieniem okresu płodnego

51%

Urbanska K., Szmidt M., Borecki M., Sysa P.

Życie Weterynaryjne

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2011

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tom 86

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nr 02

14

Cell separation with horizontal cell electrophoresis under near-isopycnic conditions on a 'density cushion'

51%

Wilk A., Urbanska K., Woolley D. E., Korohoda W.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 3

366-374

This report describes an improvement made to the horizontal cell electrophoresis methodology. It involves using two liquid layers differing in density to produce an interface described as a “density cushion”. The electrophoretic system that employed an anti-convective porous matrix to separate red blood cells (RBC) and charged dyes effectively was found to be unsuitable for some other mammalian cells. The “density cushion” method was found to be more versatile and applicable to studies on the separation of a variety of cell types. The experiments described show the differences between the electrophoretic mobilities of a human eosinophilic leukaemia cell line (Eol-1) and RBC, both with and without the modification of the cell surface properties.

15

Rola glikoproteiny P w warunkach fizjologicznych i w stanach patologicznych. Część I. Budowa chemiczna i biologiczna rola glikoproteiny P

51%

Sokolowska J., Urbanska K., Klosinska D.

Życie Weterynaryjne

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2014

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tom 89

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nr 11

16

The histology of pigmented Bomirski melanoma growing in the hamster eye - preliminary results

51%

Romanowska-Dixon B., Urbanska K., Elas M., Cichocki T.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 3

We demonstrated that a quickly growing hamster skin melanoma developed a tumor after autologous implantation into the anterior chamber of the eye. Tumor cells were seen invading all the surrounding tissues, including the iris, ciliary body, choroid and cornea. Histological examination confirmed the presence of numerous blood vessels of large diameter. Their walls were very thin, thus only the endothelium could be identified using light microscopy. Macrophages, microemboli and extravasations were present within the tumor mass.

17

Clo clu nierowne

51%

Urbanska K.

Owoce, Warzywa, Kwiaty

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1994

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nr 15

2-3

18

Glejak wielopostaciowy u psów

45%

Urbanska K., Sokolowska J., Szmidt M., Grodzik M., Sysa P.

Życie Weterynaryjne

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2011

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tom 86

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nr 10

19

Immunohistochemical detection of P-glycoprotein in various subtypes of canine lymphomas

45%

Sokolowska J., Urbanska K., Gizinski S., Zabielska K., Lechowski R.

Polish Journal of Veterinary Sciences

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2015

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tom 18

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nr 1

Combination chemiotherapy is the current standard of care for dogs with lymphoma. Multidrug resistance is one of the most important factors contributing to the efficacy of chemiotherapy. The major protein responsible for this phenomenon is P-glycoprotein. Little is known about P-glycoprotein expression in particular subtypes of lymphomas. The aim of the study was evaluation of P-glycoprotein expression in various subtypes of canine lymphomas. Positive reaction with P-glycoprotein was found in 12/25 cases of various morphological subtypes of lymphomas, however, in 3/11 lymphomas the percentage of positively weakly stained cells was <10% and those tumors were also considered negative. Tumors with 10-50% P-glycoprotein positive cells were found in single cases of centroblastic and centroblastic-centrocytic tumors. In 5 lymphomas P-glycoprotein expression exceeded 50% of tumor cells. Those cases were found among centroblastic, centroblastic-centrocytic, lymphoblastic and Burkitt-like subtypes. Positive reaction was observed mainly in the cell cytoplasm, however, in some cases prominent perinuclear dot-like staining pattern was found. In 2 cases focal staining pattern comprised dominant type of immunolabelling. Among all lymphomas containing P-glycoprotein positive cells intensity of imunolabelling was assessed as weak (6/25), moderate (2/25) and strong (3/25). Our results indicate that P-glycoprotein expression is present in nearly one third of newly diagnosed canine lymphomas of different morphological subtypes including those most commonly occurring, such as cenroblastic lymphomas. Hence, determination of P-glycoprotein expression at the time of diagnosis could provide valuable information for the design of treatment protocols. Moreover, our results have shown that P-glycoprotein expression in canine tumors could be located in Golgi-zone.

20

Fragile X mental retardation protein regulates synaptic translation of neuroligins

45%

Podsiadlowska J. J., Kuzniewska B., Milek J., Urbanska K., Dziembowska M.

Acta Neurobiologiae Experimentalis

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2017

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tom 77

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nr Suppl.1

INTRODUCTION: Neuroligins (NLGNs) are postsynaptic cell adhesion proteins which bind to their presynaptic partners neurexins across the synaptic cleft. Thus, NLGNs are crucial for the formation, maturation and maintenance of synapses. In rodents, neuroligins are encoded by four genes: Nlgn1, Nlgn2, Nlgn3 and Nlgn4. The mutations in Nlgn3 and Nlgn4 genes are associated with autistic phenotype. Another cause of autistic behaviors, fragile X syndrome, results from the lack of fragile X mental retardation protein (FMRP). FMRP binds to neuronal mRNAs and regulate local translation of transcripts that play an important role in synaptic signaling and plasticity. AIM(S): We aimed to determine if synaptic translation of Nlgn1, Nlgn2 and Nlgn3 mRNAs is regulated by FMRP. METHOD(S): We used Fmr1 knock-out mice (Fmr1 KO) and their wild type (WT) littermates to isolate synaptoneurosomes, which were stimulated in vitro to induce local protein synthesis. We performed FMRP IP on synaptoneurosomes and FISH combined with FMRP immunostaining on cultured neurons to investigate Nlgns mRNAs interaction with FMRP. The polyribosome fractionation was used to elucidate if FMRP regulates Nlgns mRNAs local translation. To study the surface versus intracellular NLGNs distribution at WT and Fmr1 KO synapses we have chosen chemical crosslinking and biotinylation assays, followed by Western blotting. RESULTS: We show that mRNAs for three studied neuroligins interact directly with FMRP in synaptoneurosomes and Nlgn1, Nlgn2, Nlgn3 mRNAs colocalize with FMRP in dendritic granules of cultured hippocampal neurons. The Nlgn1, Nlgn2 and Nlgn3 mRNAs associate with translating polyribosomes in response to synaptic stimulation and Fmr1 KO mice exhibit upregulated local translation due to the lack of FMRP. Finally, the excessive local synthesis of NLGN proteins at Fmr1 KO synapses leads to their elevated level on the postsynaptic membrane. CONCLUSIONS: Nlgn1, Nlgn2 and Nlgn3 mRNAs are locally translated at the synapse and FMRP regulates this process. FINANCIAL SUPPORT: Supported by NCN grant Sonata Bis 2014/14/E/NZ3/00375.

Ograniczanie wyników

Zastosowanie systemu ARAMIS do pomiarów odkształceń konstrukcji murowych

Modele doświadczalne w badaniach onkologicznych. Część I. Sferoidy i model in ovo

Komórki C tarczycy w warunkach fizjologicznych i stanach patologicznych. Część II. Rozrost komórek C i rak rdzeniasty

Immunohistochemical assessment of metalloproteinases MMP2 and MMP9 expression in canine various subtypes of lymphomas in relation with proliferative and apoptotic markers

Survivin expression in correlation with apoptotic activity in canine lymphomas

Kwas dokozaheksaenowy [DHA] - kluczowy skladnik tkanki nerwowej

Modele doświadczalne wykorzystywane w badaniach onkologicznych. Część II. Modele zwierzęce

Komórki C tarczycy w warunkach fizjologicznych i stanach patologicznych. Część I. Różnicowanie, morfologia, lokalizacja i funkcje komórek C

Biologiczna rola surwiwiny i jej znaczenie kliniczne

Wpływ kwasu dokozaheksaenowego na ośrodkowy układ nerwowy

Wpływ pokrycia dachu na ilość i wielkość zawiesin w spływach deszczowych

Rola glikoproteiny P w warunkach fizjologicznych i stanach patologicznych. Część II. Znaczenie glikoproteiny P w patogenezie niektórych chorób i rozwoju oporności wielolekowej

Zmienność parametrów wydzieliny dróg rodnych w przebiegu cyklu rujowego bydła ze szczególnym uwzględnieniem okresu płodnego

Cell separation with horizontal cell electrophoresis under near-isopycnic conditions on a 'density cushion'

Rola glikoproteiny P w warunkach fizjologicznych i w stanach patologicznych. Część I. Budowa chemiczna i biologiczna rola glikoproteiny P

The histology of pigmented Bomirski melanoma growing in the hamster eye - preliminary results

Clo clu nierowne

Glejak wielopostaciowy u psów

Immunohistochemical detection of P-glycoprotein in various subtypes of canine lymphomas

Fragile X mental retardation protein regulates synaptic translation of neuroligins