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  1. 19 Acta Physiologiae Plantarum

  2. 11 Acta Biologica Cracoviensia. Series Botanica. Supplement

  3. 3 Zeszyty Problemowe Postępów Nauk Rolniczych

  4. 2 Acta Biologica Cracoviensia. Series Botanica

  5. 2 Biological Letters

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  1. 44 Dubas E.

  2. 35 Zur I.

  3. 17 Krzewska M.

  4. 14 Wedzony M.

  5. 12 Golemiec E.

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  1. 2 2019

  2. 8 2018

  3. 3 2016

  4. 1 2015

  5. 6 2013

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1

Drought-induced changes in the actin cytoskeleton of barley (Hordeum vulgare L.) leaves

100%
Sniegowska-Swierk K., Dubas E., Rapacz M.
Acta Physiologiae Plantarum
|
2015
|
tom 37
|
nr 04
Plants have developed different strategies to adapt to various stress conditions including drought. In the present study the drought-induced changes in the actin filament (AFs) network was studied, for the first time, in two barley cultivars of contrasting drought tolerance level. Detached leaves of drought-tolerant (cv. ‘CAM/B1/ CI’) and drought-susceptible (cv. ‘Maresi’) cultivars were dried under controlled conditions. The water relations as well as the transcript accumulation of actin (ACT11), actin depolymerization factor (ADF1) and dehydrine HVA1) encoding genes were studied using qRT-PCR. Quantitative (the relative fluorescence index; RFI) and qualitative drought-induced changes in AF cytoskeleton were observed following staining with phalloidin. It was noticed that tolerant cultivar was characterized with relative water content decreased during drought treatment which was accompanied by increase in HVA1 expression together with decrease in ACT11 and ADF1 transcripts accumulation induced by drought. In drought-susceptible cultivar the expressions of both ACT11 and ADF1 were slightly lower than those in the control. Drought triggeredan extensive AF cytoskeleton reorganization within different types of leaf-blade cells. Remarkable changes in AF configuration and its increased amount (fluorescence intensity) were observed mainly in drought-tolerant cultivar. In addition, drought-induced changes in AFs were closely associated with chloroplasts. Those AFs probably controlled drought-induced intracellular chloroplast positioning in mesophyll. Based on the results obtained in the present study, the possible role of AF rearrangements in drought response is discussed.
2

Cytological studies on Microdochium nivale infection in Lolium perenne (L.) plants

100%
Dubas E., Marzec K., Plazek A.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2010
|
tom 545
Winter hardiness of plants is limited mainly due to sensibility to Iow temperature and winter fungus damages. Snow mould is a disease that affects many species of winter cereals, turf and forage grasses. In Poland it is caused mainly by the common and economically important fungal pathogen Microdochium nivale causing pink snow mould, destructive disease of seedlings, stem bases and ears. The aim of the study was to investigate the dynamie of Microdochium nivale infection and cytological changes in cold-hardened Lolium perenne Danish cv. Darius. Plants were inoculated with mycelium. The studied plants demonstrated fast defence response to fungus attack, accumulating phenolics already one day after inoculation, however no infecting hyphae within leaf tissue at this time were detected. The first infection hyphae in plants were observed two days after inoculation. Microscopy observations revealed sheaths of the youngest leaves located in the crowns as the main infection way. These observations showed that Microdochium nivale hyphae uptake nutrients from the host by haustoria damaging the protoplasts of mesophyll cells causing their strong plasmolysis. This type of feeding is characteristic for biotrophic and hemibiotrophic parasites. Our observation proved that Microdochium nivale is a hemibiotrophic pathogen and inoculation with its mycelium is very effective and fast and causes serious damages of Lolium perenne cells.
3

Androgenesis in isolated microspores of triticale (Xtriticossecale Wittm.) induced by stress treatments

100%
Zur I., Dubas E., Golemiec E., Wedzony M.
Acta Physiologiae Plantarum
|
2004
|
tom 26
|
nr 3 suppl.
4

Applicability of 2-DE to assess changes in the protein profile after androgenesis-inducing treatment in winter triticale (x Triticosecale Wittm.)

100%
Krzewska M., Golebiowska-Pikania G., Dubas E., Zur I.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2013
|
tom 55
|
nr 2
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Drought-induced changes of actin cytoskeleton organization in barley leaves

100%
Sniegowska-Swierk K., Dubas E., Rapacz M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2013
|
tom 94
|
nr 3
6

Chromosomy holokinetyczne u roslin

100%
Kuta E., Dubas E., Madej A.
Postępy Biologii Komórki. Suplement
|
2004
|
tom 22
115-133
7

Microdochium nivale (Fr., Samuels and Hallett): cytological analysis of the infection process in triticale (x Triticosecale Wittm.)

100%
Dubas E., Golebiowska G., Zur I., Wedzony M.
Acta Physiologiae Plantarum
|
2011
|
tom 33
|
nr 2
According to regular reports, one of the most serious diseases of winter cereal and grass varieties in moderate and cold climatic areas is pink snow mould caused by Microdochium nivale. Currently, the resistance of the economically important cereal species as triticale is not satisfactory. Moreover, there is no efficient strategy of protection against this pathogen and the understanding of plant resistance mechanisms is rather poor. Presented paper for the first time shows the cytological analysis of M. nivale infection in model triticale varieties by the use of fluorescent and light microscopy in combination with fluorescent dyes and hydrogen peroxide staining. Both, the infection level and the dynamic of the process varied for tested genotypes confirming the field and laboratory data of their different resistance to this pathogen. Moreover, our analysis showed that in both cultivars cold-hardening of seedlings delayed the mycelium growth. In both cultivars, hyphal walls and fungal penetration sites were visualized in crowns, leaf sheaths and leaves of hardened and nonhardened inoculated seedlings. For the first time the presence of the haustoria produced by M. nivale was confirmed in those tissues. Single infection hyphae usually penetrated into the host tissues via stomatal apparatuses were accompanied by the efflux of hydrogen peroxide. The data show a great potential of fluorescence techniques in studying the host plant–pathogen interactions providing a better insight into plant defence reactions that may allow elaboration of the efficient breeding strategies aimed at increasing resistance to this pathogenic fungus.
8

The cytotoxic effect of zearalenone on wheat (Triticum aestivum L.) callus cells

100%
Barbasz A., Kreczmer B., Dubas E., Pilipowicz M.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2013
|
tom 55
|
nr 2
9

Immunolocalisation techniques in plant tissues

100%
Dubas E., Szechynska M., Wedzony M.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The influence of cold pretreatment on androgenesis efficiency in isolated microspore culture of triticale (X Triticosecale Wittm.)

88%
Zur I., Godzisz I., Dubas E., Golemiec E., Wedzony M.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2006
|
tom 509
Stress was recognized as a major factor changing microspore development from a gamethophytic to a sporophytic pathway. Stress factors effectively inducing androgenesis in a wide spectrum of genotypes include high temperature, carbohydrate/nitrogen starvation and high-osmotic shocks. A low temperature treatment usually improve plant responsiveness, however in some cases, is not sufficient for embryogenesis initiation. Thus low temperature is not consider by some authors to be a true „stress factor”. In the experiment the effect of prolonged cold pretreatment of tillers on the effectiveness of androgenesis induction in isolated microspore cultures of hexaploid triticale (X Triticisecale Wittm.) was tested. In the study, two spring cultivars (cv. ‘Mieszko’ and cv. ‘Wanad’) varying in androgenic responsiveness were used. Prolongation of tillers cold pretreatment from 3 to 5 weeks, increased the number of microspores of 'Mieszko' isolated per spike from 5.8·10⁴ to 9.9·10⁴. It also promoted ELS development and its regeneration ability. ELS production increased more then 5-fold, when the number of regenerating ELS increased from 1.4% to 29%. At the same time, prolonged cold pretreatment of ‘Wanad’ tillers had no effect on the microspores yield received from one spike. Moreover, it reduced the number of produced ELS about 3-fold. Due to great variation among replicates the increase of ELS regeneration ability (from 2.8% to 24.5%) was not statistically significant. For both cultivars, the highest green plant regeneration percentage (24% and 8.2% for ‘Wanad’ and ‘Mieszko’, respectively) was noted after the longest cold pretreatment of tillers. However, the great variation among replicates made the comparison among treatments difficult for statistical analyses. Proper interpretation of the received results needs a further and more detailed examination.
11

The involvement of endogenous auxins in androgenesis of Brassica napus

88%
Dubas E., Benkova E., Waligorski P., Dziurka M., Zur I.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2012
|
tom 54
|
nr 1
12

Stress as a trigger for androgenesis induction in isolated microspore cultures of wheat (Triticum aestivum L.)

88%
Zur I., Dubas E., Golemiec E., Wedzony M., Sabatowska M.
Acta Physiologiae Plantarum
|
2003
|
tom 25
|
nr 3 suppl.
13
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Cell structural reorganization during induction of androgenesis in isolated microspore cultures of triticale [xTriticosecale Wittm.]

88%
Dubas E., Wedzony M., Petrovska B., Salaj J., Zur I.
Acta Biologica Cracoviensia. Series Botanica
|
2010
|
tom 52
|
nr 1
73-86
Upon stress treatment, isolated microspores of triticale (×Triticosecale Wittm.) were directed towards sporophytic development (androgenesis). We used fluorescence microscopy to study the cell structural reorganization associated with the process. Changes in the developmental pathway coincided with the character of the microtubular cytoskeleton configuration, the number and direction of nuclear divisions, changes in vacuolization, the distribution of mitochondria, ER and starch grains, and the architecture of new cell wall formation. A band of diffused fluorescence surrounding the nucleus was observed before the first symmetric division of microspores. This structure most likely represents a preprophase band (PPB). Successive mitotic divisions within the microspore wall led to the formation of multinucleate or multicellular structures consisting of one or two domains of cells differing in size. They were later released from the sporoderm and continued further development with features typical for a monocotyledonous embryo. The pattern of internal architecture of androgenic structures depended on their developmental phase. Before and after release from the microspore wall, cortical microtubules (MTs) exhibited various configurations without preferential orientation. They formed a denser network in the region opposite to the sporoderm rupture site. Released multicellular structures showed both intensely fluorescing cortical MTs and more dispersed endoplasmic MTs radiating along the cytoplasmic strands from the nuclear region to the cell cortex. Up to globular stage, isotropically expanding cells of androgenic embryos showed a random pattern of MTs. This is the first report that successive events of androgenic development of triticale microspores are associated with MT reorganization. The results support the view that changes in cytoskeleton architecture are critical during induction of androgenesis.
14

Increasing tolerance to abiotic stresses by haplodiploidization in Hordeum vulgare L.

88%
Malaga S., Dubas E., Krzewska M., Nowicka A., Zur I.
Acta Physiologiae Plantarum
|
2016
|
tom 38
|
nr 01-10th International Conference Plant Functioning under Environmental Stress...
15
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Stress as a trigger for androgenesis induction in isolated microspore culture of wheat (Triticum aestivum L.)

88%
Zur I., Dubas E., Golemiec E., Wedzony M., Sabatowska M.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2004
|
tom 496
|
nr 2
The effect of various stress treatments (cold, heat, starvation) and its combinations on androgenesis induction in isolated microspore cultures of spring wheat (Triticum aestivum L.) was under the study. On the basis of our preliminary experiments, spring wheat cultivar Minaret recognized as responsive for androgenic development was selected and used in the study. The anthers were aseptically isolated from freshly cut or cold-pretreated (9-14 days at 4°C) tillers. The anthers were precultured in starvation B medium or rich AT3 medium at 5, 26 or 33°C. After 4 days, microspores were mechanically isolated and cultured in AT3 medium at a density 1 x 10⁵ microspores per cm³, at 26°C, in the dark. Well-developed embryos were transferred to solid 190-2 medium and cultured at 26°C, in the dim light. Cytological observations were done in 2-weeks intervals with inverted light microscope. The analysis of the number of spontaneously shed microspores and its cytological characterization conducted after 4-day anther pre-culture was a good indicator of culture androgenic potential. The effectiveness of androgenesis induction was depended on the type and sequence of stress treatments. The highest intensity of stress treatment (combination of cold pretreatment of tillers with heat and carbohydrate/nitrogen starvation stresses applied to anthers) induced the most effective androgenic development and as result a great number (about 100 per dish) of multicellular androgenic structures were formed. However, the final effect (the number of globular embryos big enough to be transferred onto regeneration medium) was not satisfying (max 50 embryos per dish). Cold shock applied both to tillers or anthers enhanced androgenic development and plant regeneration. The percentage of regenerating embryos ranging from 0 to 50%. However all regenerates were chlorophyll-lacking (albino) plants. In the control cultures (no stress treatment) microspores continued their normal gametophytic development.
16

Visualisation of microtubules and actin filaments in fixed BY-2 suspension cell

88%
Szechynska-Hebda M., Wedzony M., Dubas E., Emons A. M., Lammeren A.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
17

Karyology and amounts of nuclear DNA in Luzula L.- A genus with holokinetic chromosomes

88%
Dubas E., Kuta E., Bohanec B., Vizintin L., Przywara L.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
18

Influence of growth regulators level on the initial phases of androgenic induction in isolated microspore culture of triticale (x Triticosecale Wittm.)

76%
Zur I., Dubas E., Filek M., Biesaga-Koscielniak J., Golemiec E., Wedzony M.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2005
|
tom 47
|
nr 1
19

Stress proteins play important role in microspore embryogenesis induction in triticale (× TriticosecaleWittm.)

76%
Krzewska M., Dubas E., Golebiowska-Pikania G., Malaga S., Nowicka A., Zur I.
Acta Physiologiae Plantarum
|
2016
|
tom 38
|
nr 01-10th International Conference Plant Functioning under Environmental Stress...
20

Quantitative trait loci associated with androgenic responsiveness in triticale (×Triticosecale Wittm.) anther culture

76%
Krzewska M., Dubas E., Czyczylo-Mysza I., Golebiowska-Pikania G., Stojalowski S., Zur I.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2013
|
tom 55
|
nr 2
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