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1

Fluorki w zebach lisa Vulpes vulpes z Pomorza Zachodniego

100%
Kalisinska E., Palczewska M.
Ochrona Środowiska i Zasobów Naturalnych
|
2007
|
nr 31
428-433
2

Cation binding properties of calretinin, an EF-hand calcium-binding protein

100%
Groves P., Palczewska M.
Acta Biochimica Polonica
|
2001
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tom 48
|
nr 1
Calretinin (CR) is a neuronal EF-hand protein previously characterized as a calcium (micromolar affinity) binding protein. CR-containing neurons are spared in some neurodegenerative diseases, although it is as yet unconfirmed how CR plays an active role in this protection. Higher levels of some metal cations (e.g. copper and zinc) are associated with these diseases. At the same time, metals such as terbium (NMR and fluorescence) cadmium (NMR) and manganese (EPR) serve as useful calcium analogues in the study of EF-hand proteins. We survey the binding of the above-mentioned metal cations that might affect the structure and function of CR. Competitive 45Ca2+ -overlay, competitive terbium fluorescence and intrinsic tryptophan fluorescence are used to detect the binding of metal cations to CR. Terbium and copper (half-maximal effect of 15 uM) bind to CR. Terbium has a similar or greater affinity for the calcium-binding sites of CR than calcium. Copper quenches the fluorescence of terbium-bound CR, and CR tryptophan residues and com­petes weakly for 45Ca2+ -binding sites. Cadmium, magnesium, manganese and zinc bind less strongly (half-maximal effects above 0.1 mM). Therefore, only terbium appears to be a suitable analytical calcium analogue in further studies of CR. The principal conclusion of this work is that copper, in addition to calcium, might be a factor in the function of CR and a link between CR and neurodegenerative diseases.
3

Metal cations decrease the calcium-binding capacity of neuronal calretinin

81%
Groves P., Palczewska M., Kuznicki J.
Acta Neurobiologiae Experimentalis
|
1999
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tom 59
|
nr 3
4

Zastosowanie PCR w diagnostyce wirusa czarnej pierscieniowej plamistosci pomidora [Tomato Black Ring Nepovirus]

81%
Palczewska M., Borodynko N., Pospieszny H.
Progress in Plant Protection
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2001
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tom 41
|
nr 2
699-702
5

Serologiczne zroznicowanie polskich szczepow wirusa czarnej pierscieniowej plamistosci pomidora [Tomato Black Ring Nepovirus /TBRV/]

81%
Borodynko N., Palczewska M., Pospieszny H.
Progress in Plant Protection
|
2001
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tom 41
|
nr 2
696-698
6

Use of plant extracts to control and treat AB5 enterotoxin-related diarrhea

81%
Komiazyk M., Palczewska M., Sitkiewicz I., Groves P.
Polish Journal of Microbiology
|
2014
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tom 63
|
nr 1
Plants contain a broad spectrum of small molecules with potential antimicrobial properties. Here, we review the antimicrobial activities of plant extracts against enterotoxic bacteria encoding AB5 toxins, including Vibrio cholerae, Shigella dysenteriae and enterotoxic Escherichia coli strains. Several plant extracts have strong antimicrobial effects and the potential to boost Oral Rehydration Therapy, which is the first line of treatment for acute diarrhea.
7

Occurrence of pepino mosaic virus in Poland

80%
Pospieszny H., Borodynko N., Palczewska M.
Phytopathologia Polonica
|
2002
|
tom 26
8
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Partial characterization of Sunn-hemp mosaic tobamovirus [SHMV] isolated from bean plants [Phaseolus vulgaris L.]

80%
Pospieszny H., Palczewska M., Borodynko N.
Journal of Plant Protection Research
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2001
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tom 41
|
nr 2
This work presents some properties of Sunn-hemp mosaic tobamovirus (SHMV) orginally isolated from bean plants. Virus infected host range and induced symptoms that were typical for SHMV. Following plant species distinquished SHMV from tobacco mosaic tobamovirus (TMV): Phaseolus vulgaris, Pisum sativum, Lupinus albus and Lycopersicon esculentum. In immunoblotting the serum against SHMV did not react with TMV and Tomato mosaic tobamovirus (ToMV). The electrophoretical patterns of whole virions and capsid proteins were characteristic for SHMV and different from that of TMV and ToMV.
9

Concanavalin A-agarose removes mannan impurities from an extracellularly expressed Pichia pastoris recombinant protein

80%
Palczewska M., Batta G., Groves P.
Cellular and Molecular Biology Letters
|
2003
|
tom 08
|
nr 3
Pichia pastoris secretes few native proteins. However, the more than 1 g 1-1 of extracellularly expressed mannan interfered with the purification of our extracellularly expressed, non-glycosylated recombinant protein. Concanavalin A-agarose removed more than 95% of the unwanted mannan as monitored by phenol reaction. A 13C-based NMR assay confirmed this improvement. Concanavalin A-agarose can assist the purification of extracellular expressed, non-glycosylated proteins from yeasts.
10

Plant polyphenols prevent the deleterious effects of Shiga-like enterotoxins

61%
Komiazyk M., Gasik J., Palczewska M., Biesaga M., Pikula S., Groves P.
Acta Biochimica Polonica
|
2018
|
tom 65
|
nr S2
11

Peptide-based biopolymers PolyRGD to be used for pro-regenerative purposes

61%
Zylicz-Stachula A., Zebrowska J., Krawczun N., Palczewska M., Mucha P., Skowron P. M.
Acta Biochimica Polonica
|
2018
|
tom 65
|
nr S2
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