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W przeprowadzonym doświadczeniu zbadano wpływ dwóch typów fruktanów o różnym stopniu polimeryzacji łańcucha węglowodanowego: krótkołańcuchowych fruktooligosacharydów (FOS) oraz długołańcuchowej inuliny na fizjologię jelita ślepego u szczurów. W badaniach zastosowano trzy diety: dietę kontrolną zawierającą 7,5% sacharozy i dwie diety doświadczalne zawierające: 8,3% preparatu inuliny (IN) i 7,9% preparatu FOS (FOS) (preparaty komercyjne). Młode samce rasy Wistar (8 osobników w grupie) otrzymywały diety ad libitum przez 4 tygodnie. Określono indywidualne spożycie paszy oraz przyrost masy ciała. Pomiarowi podlegały m.in.: masa tkanki i treści jelita ślepego, pH, sucha masa, amoniak, białko, lotne kwasy tłuszczowe (LKT) i aktywność enzymów bakteryjnych w treści jelita ślepego. Nie stwierdzono statystycznie istotnej różnicy pomiędzy grupami w wielkości spożycia diet oraz przyrostu masy ciała. Spożycie diet zawierających FOS i inulinę spowodowało znaczny wzrost masy ściany i treści jelita ślepego (P < 0,05) w stosunku do grupy kontrolnej. W grupach doświadczalnych stwierdzono także wzrost koncentracji białka oraz suchej masy w treści jelitowej. Istotnie niższe (P < 0,05) wartości pH i koncentrację amoniaku odnotowano u zwierząt żywionych dietami z FOS i inuliną. Suplementacja diet fruktanami spowodowała wzrost zawartości LKT w przeliczeni na 100 g masy ciała (pula LKT). Inulina, korzystniej niż FOS wpływała na produkcję LKT, zwłaszcza istotnie zwiększając zawartość kwasu propionowego i masłowego w treści jelita ślepego. Inulina w sposób bardziej korzystny niż FOS oddziaływała również na aktywność enzymatyczną mikroflory jelita ślepego. W grupie IN obserwowano najniższą aktywność β-glukuronidazy, która jest biomarkerem aktywności mikroflory patogennej.
W wykonanym doświadczeniu badano wpływ fruktanów i polifenoli, równocześnie występujących w diecie, na funkcjonowanie końcowego odcinka przewodu pokarmowego u szczurów doświadczalnych. Zastosowano trzy diety, w tym kontrolną zawierającą 7,5 % sacharozy oraz dwie doświadczalne zawierające: 1) 7,9 % komercyjnego preparatu fruktooligosacharydów (FOS) 2) 8,3 % koncentratu FOS uzyskiwanego z korzeni cykorii, który równocześnie wprowadzał do diety polifenole na poziomie 0,0 5% (FOS+PP). Młode samce rasy Wistar (8 osobników w grupie) otrzymywały paszę ad libitum przez 4 tygodnie. Określono indywidualne spożycie paszy oraz przyrost masy ciała. W treści jelita ślepego oznaczano pH, zawartość: suchej masy, amoniaku, białka, lotnych kwasów tłuszczowych (LKT) oraz aktywność enzymów bakteryjnych. Ważono również jelito ślepe z treścią i bez treści. Nie stwierdzono statystycznie istotnej różnicy pomiędzy grupami pod względem wielkości spożycia paszy oraz przyrostu masy ciała. Spożycie pasz zawierających preparat FOS komercyjny i preparat FOS+PP z korzeni cykorii spowodowało znaczny wzrost masy ściany i treści jelita ślepego (P<0,05) w stosunku do grupy kontrolnej. Istotnie niższe (P < 0,05) wartości pH i mniejsze stężenie amoniaku w treści jelita ślepego odnotowano u zwierząt żywionych paszami doświadczalnymi. Badane preparaty istotnie podwyższyły pulę LKT, zwłaszcza istotnie zwiększając zawartość kwasu propionowego i masłowego w przeliczeni na 100 g masy ciała. W grupie FOS+PP odnotowano istotny wzrost koncentracji LKT w treści jelita ślepego (P < 0,05 vs grupy FOS i K). Diety doświadczalne korzystnie modyfikowały aktywność bakteryjnej -glukozydazy oraz -glukuronidazy.
The aim of the study was to compare the effect of two types of fructans characterised by a different degree of polymerization (long-chain inulin and short-chain fructooligosaccharides [FOS]), added as single dietary components on the activity of selected bacterial enzymes in the faeces and the caecal digesta, the antioxidant status and the serum lipid profile in laboratory rats. Twenty four male Wistar rats were randomly divided into three groups and for 4 weeks were fed a control casein and two experimental diets supplemented with fructooligosaccharides and inulin at a level of 7.5% of diet (C, FOS and IN groups, respectively). The inulin addition caused a significant decrease in faecal pH value throughout the study (pH measurements were taken after day 7, 14, 21 and 28 of feeding), whereas the FOS-diet significantly decreased pH of fresh faeces only after 3 weeks of the study (p<0.05 vs. the control). Both experimental diets beneficially diminished the activity of faecal β-glucosidase and β-glucuronidase as compared to the control animals. The decreased activity of bacterial β-glucosidase and increased activity of β-galactosidase in the caecal digesta was noted only in the case of dietary FOS. The lowest concentrations of TBARS in the liver, kidneys and serum were observed in the IN group (p<0.05 vs. the control), however the FOS diet also effectively reduced concentration of TBARS in the liver tissue and the serum. The dietary inulin significantly decreased the serum glucose level and increased the percentage of HDL-cholesterol in total cholesterol (vs. C and FOS groups). The results obtained demonstrate that the consumption of diet supplemented with inulin seems to be more health promoting and elicits more beneficial changes in microflora activity, lipid metabolism and antioxidant status in rats than that enriched with short-chain fructooligosaccharides.
The aim of the study was to characterise caecal indices in rats fed diets supplemented with high doses (0.4, 0.8 and 1.2%) of a grapefruit commercial phenolic extract. Addition of the preparation caused an increase of wet mass of ceacal digesta. Dry matter content decreased from 22% to 7.0% and pH increased from 7.11 to 8.0 in the control group and experimental groups, respectively. Increased pH was a consequence of the lower concentration of volatile fatty acids in rats fed the extract (77 μmol/g in control animals versus 8-13 μmol/g in extract treatments). However, glycolytic microbiological activity, measured indirectly through the measurement of its enzyme activity, decreased only in rats fed a diet containing the lowest level of the preparation.
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Metabolic effects of dietary apple seed oil in rats

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The aim of this study was to determine the fatty acid profile of apple seed oil and its effects on the caecal functions, blood lipids, and markers of antioxidant status and inflammation in rats. A nutritional experiment was performed on Wistar rats allocated to 3 groups of 8 animals each. The animals were fed with a diet containing different sources of fat: pork lard (group LA), rapeseed oil (group RO) and apple seed oil (group AO). Apple seed oil was rich in linoleic acid and oleic acids (57 % and 32.3 % of total fatty acids, respectively). The short chain fatty acid concentration in the caecal digesta was comparable among all groups, whereas the ammonia concentration was lower in groups AO and RO than in group LA (0.32 and 0.3, respectively vs 0.42 mg/g). The plasma alanine (ALT) and aspartate transaminase (AST) activities also decreased in the AO and RO groups (ALT, 19.34 and 19.81, respectively vs 30,7 U/L and AST, 115.1 and 107, respectively vs 138.3 U/L) The plasma triacylglycerols (TG) concentration and the atherogenic index (ATI) of plasma were significantly decreased in the AO group compared to the LA group (TG, 1.79 vs 2.62 mmol/L and ATI, 0.095 vs 0.313). Apple seed oil is a valuable source of unsaturated fatty acids and its dietary addition has slightly better metabolic effects on rat organism than does rapeseed oil.
The objective of this study was to determine the effect of different dietary sodium levels on serum macroelement concentrations, growth performance and incidence of foot pad dermatitis (FPD) in female turkeys raised to six weeks of age. The influence of a lowsodium diet (without additional sodium) and diets supplemented with NaCl to increase sodium content by 0.5, 1.0, 1.5, 2.0 and 2.5 g kg–1 was compared. The dietary electrolyte balance (DEB) was high (242-248 mEq kg–1) due to a high potassium content (11.0 g kg–1). Turkeys fed the low-sodium diet were characterized by significantly lower concentrations of chloride (p=0.002) and phosphorus (p<0.001), and significantly higher magnesium levels (p<0.001), compared with the other groups. The lowest dietary inclusion of sodium (0.5 g kg–1) contributed to a significant increase in the body weights of turkeys at six weeks of age (p<0.001), and it improved feed conversion efficiency (p<0.001) in comparison with the low-sodium diet. No significant differences in body weight and feed conversion ratio followed experimental treatments with higher dietary NaCl addition. Diets supplemented with different amounts of sodium had no effect on litter moisture content, whereas the incidence of FPD increased significantly following the addition of dietary sodium at 1.0 g kg–1 to 2.5 g kg–1, compared with the low-sodium diet. The results of our study indicate that moderate sodium supplementation (0.5 g kg–1) of a sodium-deficient diet significantly increased the body weights of young turkeys, while it did not increase the incidence of FPD. A further increase in the sodium content of experimental diets did not increase the body weights of birds, and it enhanced the occurrence of FPD which was not related to increased litter wetness.
Summary In the article the factors that stimulate the growth of the turkey gastrointestinal tract were reviewed. Based on data derived from literature and results of the authors’ own investigation it could be concluded that an effective way to increase the gastrointestinal tract (GIT) development is through the early access of turkey chicks to feed. During the period of growth, morphological development of GIT can be stimulated through appropriate feed additives that support beneficial intestinal microflora. A moderate increase of crude fiber in a concentrate mixture (up to 6%) influences the capacity and structure of the GIT of turkeys to a lesser extent.
The objective of this study was to determine the response of broiler chickens to different dietary sodium (Na) levels. The experiment was performed on 432 male Ross 308 chickens, kept in three-tier battery cages, each with a floor area of 0.5 m2. The chickens were divided into six experimental groups of eight replicates, each of nine birds. Six experimental diets were prepared: a basal diet without additional Na source and diets supplemented with NaCl in the amount of 0.52, 1.03, 1.54, 2.05 and 2.54 g/kg in the starter period (1-14 days) and 1.27, 2.54, 3.82, 5.09 and 6.36 g/kg in the grower period (15-35 days). The dietary Na, Cl and K content was 0.22-2.61, 1.11-4.81 and 8.76-9.61 g/kg, respectively. Dietary electrolyte balance (DEB), defined as Na+ + K+ - Cl-, averaged 210 mEq/kg in all diets. The Na content of basal diets was very low (0.33 g/kg in starter diets and 0.22 g/kg in grower diets). It inhibited the growth of broilers despite a high DEB resulting from a high K content (about 9 g/kg). The addition of NaCl to starter diets significantly increased the body weight (BW) of chickens, but only to the amount of 1.1 g Na per kg feed. The addition of 2.54 g NaCl per kg grower diets increased the Na and Cl content of the diet to 1.16 g/kg and 2.68 g/kg, respectively, leading to a significant improvement in overall production results, slaughter value and tibia mineralization, without negative effect on excreta moisture. A further increase in the Na content of grower diets (to 1.69, 2.18 and 2.61 g/kg, respectively), accompanied by an increase in Cl concentrations (to 3.38, 4.11 and 4.81 g/kg, respectively) did not increase the BW of chickens, feed conversion efficiency and slaughter value, while it increased excreta moisture and decreased parameters characterizing the mineralization and elasticity of the tibia in birds fed a diet with the highest Na content (2.61 g/kg). As compared to the group fed the basal diet, the medium and highest Na dosages significantly decreased pH and dry matter concentration of small intestine contents; the treatment with the highest dietary Na levels stimulated bacterial β-glucosidase and β-glucuronidase, yet it did not increase the caecal short-chain fatty acids concentration.
The objective of this study was to determine the effect of different dietary levels of sodium in diets with and without sodium chloride on mineral metabolism, including blood electrolyte levels and tibia mineralization parameters, in young turkeys (to six weeks of age). The influence of diets with a low (L), medium (M) and high (H) sodium content, at 0.34, 1.34 and 2.82 g/kg respectively, was compared. The content of chloride and potassium in turkey diets (1.7 - 5.9 and 11 g/kg, respectively) was above the recommended levels. The sodium-deficient diet L decreased the serum concentrations of sodium, chloride and phosphorus, and it increased the serum levels of calcium and magnesium in turkeys, compared with groups M and H. Relative to group L, different dietary sodium levels in groups M and H contributed to a similar increase in the body weights of birds (1.06 vs. 1.46 and 1.44 kg, p<0.001) and in the absolute (4.60 vs. 6.83 and 6.62 g, p<0.001) and relative tibia weight (0.42 vs. 0.46 and 0.46% body weight, p=0.031). No significant differences were found between groups with respect to the content of ash, calcium and phosphorus in tibia dry matter. Supplemental sodium increased the bone density index (from 50.6 to 68.4 and 66.3 mg/mm in groups L, M and H, respectively, p<0.001), the maximum bending moment (from 5.27 to 7.40 and 7.33 N/mm, p=0.002) and the minimum breaking strength of tibia (from 0.136 to 0.191 and 0.189, p=0.002). In conclusion, our study indicates that the applied dietary treatment with a moderate sodium level (1.34 g/kg) resulted in a rate of bird growth and tibia mineralization similar to those observed with the treatment with much higher Na content (2.82 g/kg).
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