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EZ-Fluo™ System from Merck Millipore significantly reduces the time of determi nation of microbial contamination of juices and beverages compared to standard membrane filtration method. The time of yeasts incubation was shortened from 120 to 30–40 hours, depending on the tested species. Lactic acid bacteria were enumerated by EZ-Fluo™ System after 24–28 hours, depending on the species, while the standard incubation takes 72 hours. The time required to enumeration of Alicyclobacillus acidoterrestris count was 16 hours, and Alicyclobacillus acidocaldarius 40 hours, instead of 120 hours necessary in the standard method. Analysis of naturally contaminated samples requires a slightly longer incubation time than that determined for the tested strains.
The objective of the work was to establish the effect of an ovaries hand massage on the reproductive activity and hormonal changes during winter anestrus and luteal phase of estrous in mares. The examinations were done on 10 mares (Polish pony) in January 1992 during winter anestrus and in August 1993 during luteal phase of estrous cycle (diestrus), divided into 2 groups: experimental and control. In the experimental group ovaries were massaged ever day for 30 sec. Sexual behavior of mares was checked every day with the help of a stalion, ovaries were examined per rectum and by USG. The level of progesterone and estradiol was determined every second day in venous blood (from the external jugular vein). It was found that ovarial massage did not significantly affect reproductive activity and hormonal activity of ovaries during winter anestrus. A possibility of shortening of estrus during reproductive season during the luteal phase of estrous (diestrus) was found.
Alicyclobacillus spp. is an important thermoacidophilic, spore-forming bacterium that is a major concern for beverage and juice industries. This study was undertaken to evaluate the reliability of the results obtained according to IFU No. 12 detection method, widely used in Europe. The filtration method, in comparison to the enrichment method, increased the likelihood of detecting Alicyclobacillus contamination of fruit juice concentrates. The enrichment method with a dilution ratio of apple juice concentrates of 1:10, gives about 50% false-negative results, whereas for dark berry juice concentrates, with dilution ratio of 1:50, approximately 43% false-negative results was achieved.
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