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The paper presents the data associated with non-specific reactions of sera tested for equine infectious anaemia (EIA). The animals before samples collection suffered from influenza. Out of 502 sera assessed by the double immunodiffusion test 20 reacted positively with EIA antigen developing single non-specific precipitation lines. It was found that the process of incubation, freezing and thawing of the sera brought about a vane of precipitation. These sera possessed a statistically significant increase of proteins of acute phase (seromucus, ceruloplasmine and sialic acids) compared with the control group. No such changes were found by means of haematological examinations.
The aim of the research was to assess changes in ghrelin, leptin and free fatty acids (FFA) levels in women’s blood after training. The research was carried out in women aged 45.55 ±11.33 years and with the BMI of 26.49 ±4.49. Health training at 50–66% VO2max took place twice a week for 9 months. In the baseline phase and in the 3rd, 6th and 9th month of the training, body mass and composition were measured, cardiorespiratory fitness was checked after a 10-minute exercise test on a cycloergometer, and fasting levels of ghrelin, leptin and FFA in the serum were assayed and 15 minutes after the exercise test. Body mass was reduced in the 6th month of the training. Fasting ghrelin level increased because of training, leptin and FFA decreased. After single 10-minute exercises performed every 3 months level of ghrelin and FFA increased while leptin decreased. An increase in ghrelin level in the blood after the single exercise can be the result of negative energy expenditure. An increase in fasting ghrelin level after training can be one of the adaptive physiological mechanisms connected with energy saving. A mechanism that is switched on as a result of a long-lasting stimulus that leads to energy losses, reduction in body mass and a decrease in leptin level in the blood.
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