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The aim of the performed investigations was to evaluate the cadmium content in the kidneys and liver of free-living European bisons in the Bia³owie¿a forest. The cadmium content was determined in the kidneys and livers of 20 animals (14 females, 6 males; 15 calves of up to one year of age and 5 animals older than 2 years). Cadmium was analysed by the ICP-OES method in an accredited laboratory; after homogenisation, samples were mineralised in a microwave apparatus in Teflon® containers, under pressure in nitric acid. The obtained results are presented in Tables 1-3. The mean value of cadmium in the kidneys amounted to 1.82 and in the liver to 0.83 mg/kg of fresh tissue. No significant sex-dependant differences were observed. However, significant differences were noted depending on the animal’s age, older animals cumulating more cadmium both in the kidneys and liver.
The study aimed at assessing the sodium and potassium content in chosen tissues and organs of free-ranging European bisons in Białowieża Primeval Forest depending on the gender and age of animals. In order to determine the content of elements in parenchymal tissues, ribs and hair, the ICP-OES method was used. In the hooves, sodium and potassium were determined with the help of ICP-MS. The sodium content in organs and skin appendages varied from 0.30 in hair to 4.77 mg g-1 in ribs. In the analysis of the age effect, some significant differences were observed between the investigated groups in the sodium content of the hoof wall, namely, a higher mean value was noted in young individuals. The potassium content in the examined samples was within the range of 0.96 in the hoof wall to 3.63 mg g-1 in kidneys. Significant age dependent differences were noted only in the ribs. Sodium and potassium concentrations in the hoof wall were correlated in a highly significant way. Similar dependences also appeared between the content of sodium and potassium in kidneys and liver and kidneys and muscles. On the basis of the results it can be concluded that the status of sodium and potassium supply in the European bison from Białowieża Forest is adequate.
Rubidium is an alkaline metal with exceptional chemical activity. Together with lithium, sodium, potassium and cesium, it belongs to group I of the periodic table of elements. The aim of this study was to provide data on the rubidium content in normal mammary gland tissue and adenocarcinomas and to elucidate the effects of altered homeostasis on this element in the course of mammary gland neoplastic disease in dogs. The investigation was performed to confirm the hypothesis put forward in human research that this metal is cumulated in neoplastic tumours of the mammary gland. The research material included post mortem collected healthy mammary glands of bitches and mammary gland neoplastic tumours obtained during routine surgical treatments as well as during necropsies. The content of rubidium was determined by the inductively coupled plasma mass spectrometry (ICP-MS) method. The highest rubidium content was observed in the tissues with neoplastic lesions, where it averaged 10.72 mg kg–1 wet mass. In the healthy tissues, the content of this metal was on a much lower level. The mean rubidium concentration in mammary glands without pathological changes was 1.91 m kg–1 wet mass. The statistical analysis showed a significant difference (P=0.01) in the rubidium content between the investigated groups. Higher rubidium concentrations were observed in the tissues with neoplastic lesions. This phenomenon is similar to disturbances of the ionic balance observed in the case of breast cancer in women.
The objective of the presented study was to compare rubidium concentration in different organs of bitches, depending on their health state. Samples of muscles, liver, and kidneys collected post mortem from 45 animals, at the age of 1-8 years, were examined. Chemical analysis was conducted using the inductively coupled plasma atomic emission spectroscopy method. The contents of rubidium in particular organs varied from 2.07 to 4.52, from 1.25 to 3.81, and from 1.32 to 3.78 mg‧kg⁻¹ of wet weight for muscle, kidney, and liver samples, respectively. The highest values were observed in animals with neoplastic disease and the lowest in healthy animals. These differences were highly statistically significant.
The aim of the investigation was to confirm the hypothesis put forward in human research that copper is cumulated in the neoplastic tumours of the mammary gland. The research material included the post mortem collected healthy mammary glands of bitches and mammary gland neoplastic tumours obtained during routine surgical treatments. The histopathological examinations revealed that among tumours of epithelial origin, the most frequent were adenocarcinomas, which comprised 64% of all neoplastic lesions. The second group included carcinomas - 26%, whereas 10% were the tumours of mesenchymal origin. The lowest copper content was observed in healthy mammary glands. In the tissues with neoplastic lesions, the content of copper was at a much higher level. Statistical analysis revealed a significant difference (P≤0.05) in the copper content between the investigated groups. The performed analyses showed that mammary gland neoplasms cumulate copper ions, and a much higher concentration of this element is observed in the tumours of epithelial origin.
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