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Eight male rats copulated with an estrus female with an open vagina (NF) and an estrus female with the vagina covered by adhesive tape (CF). Experimental sessions were composed of two phases. During Phase 1 the males copulated with NF or with CF females until 4 intromissions or 4 mount bouts were displayed respectively. In Phase 2 the males copulated alternately with NF or CF according to three schedules: (1) a sequence two mount bouts with a CF female followed by one intromission, (2) a sequence one intromission followed by two mount bouts with a CF female , and (3) a sequence one intromission followed by four mount bouts with a CF female. All of these sequences were repeated until ejaculation. Ejaculation occurred in 95.8% of cases within one minute after the last intromission during copulation with the CF female. The results of this study indicate that: (1) the number of intromissions displayed during Phase 2 does not depend on the character of the copulatory behaviour displayed during Phase 1, (2) at least two intromissions are displayed before ejaculation, and (3) the tactile stimulation of the glans of penis plays a crucial role in the ejaculatory seminal reflex.
Sexual behavior of male rats is well described type of appetitive behavior. This behavior is described by at least five independent factors: anticipatory, initiation, copulatory rate, hit rate and intromission count. Each factor has specific neuronal networks. The aim of our study was to analyze which factor is the most informative to describe sexual deficit in rats with depressive like syndrome. We investigate 6–8 month old WAG/Rij rats with absent epilepsy commodity with moderate depression and compare sexual behavior with control Crl:Han Wistar and Sprague-Dawley males. Sexually naïve WAG/Rij rats started to copulation less frequently (only 6 from 20 display mounting) compare to Crl:Han Wistar (6 from 6) and Sprague-Dawley (4 from 6). Sexually experienced WAG/Rij rats displayed longer mount latency (initiation factor) compare to both control strains. Surprisingly, sexually experienced WAG/Rij rats vocalized very intensively at 50-kHz band during five minutes before introduction of female. This anticipatory precontact vocalizations did not correlate with initiation of copulation. Latency to initiation of copulation (initiation factor) seems to be the most sensitive parameter describing depressive like syndrome during male rat sexual behavior and seems to be useful to investigation depressive disorders. It correspond to lower libido in men depressive patients.
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