Wyniki wyszukiwania

1

Leuzea carthamoides DC. in vitro

100%

Duskova J., Dusek J.

Herba Polonica

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1995

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tom 41

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nr 4

165-169

Tissue cultures were derived from the roots and above-ground part of sterile seedlings of Leuzea carthamoicles DC. Testing of the growth on the medium according to Murashige and Skoog with the addition of various combinations and concentrations of growth substances proved that at calluses derived from the aerial part the highest stimulative effect was carried out by the combination of 2,4-D (1.0 mg1-1) and IBA (0.5 mg1-1). The average value of the increment was 544%. Callus cultures derived from the roots grew less intensively than the cultures derived from the aerial parts. The highest stimulative effect was found at 2,4-D (1.0 mg1-1) in combination with BA (1.0 mg1-1) - 230%. During experiments for a certain level of morphological differentiation bud-formation was reached and after rooting plant regeneration. When substituting 1AA by Meristimul and its combination with kinetin as well, deformed buds were formed. Orientation chromatographic analysis of callus extracts and leaves of regenerated plants has not proved the presence of ecdysteroids so far.

2

Zur biotransformation von Hydrochinon zu Arbutin in den in Vitro-Kulturen

81%

Duskova J., Dusek J., Jahodar L.

Herba Polonica

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1999

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tom 45

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nr 1

23-26

Badania dotyczą możliwości wytwarzania arbutyny w hodowlach tkankowych Bellis perennis L., Bergenia crassifolia (L.) Fritsch, Brassica oleracea var. Capitata L., Coronilla varia L., Leonurus cardiaca L., Leuzea carthamoides DC., Rheumpalmatum L., Rhodiola rosea L. i Datura meteloides DC. ex Dunal z jej potencjalnych prekursorów (hydrochinon, kwas 4-hydroksybenzoesowy, tyrozyna) w podłożu hodowlanym. Optymalna przemiana hydrochinonu i kwasu 4-hydroksybenzo- esowego do arbutyny zachodziła w hodowli tkankowej Datura meteloides, a tyrozyny do arbutyny w hodowli Rhodiola rosea. Procesy te odnoszą się do określonego czasu i aktywności działania prekursorów. Arbutyna nie była usuwana w trakcie doświadczeń z podłoża.

3

Possibilities of using medicinal plants from the area exposed to lead

81%

Dusek J., Tumova L., Duskova J.

Herba Polonica

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1995

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tom 41

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nr 2

77-79

The aim of this paper was to study the contamination of Flos Taraxaci, Folium Taraxaci, Folium Plantaginis and Flos Sambuci by lead. The raw materials were collected from places situated near the cross roads. From all herbs there were prepared decoctions, infusions and teae. The lead content in all preparations met regulations for the drinking water valied in the Czech Republic, hi case of decoctions and infusions, howewer, it exceeded the standards for baby water.

4

Chitinolytic enzymes from bacterium inhabiting human gastrointestinal tract - critical parameters of protein isolation from anaerobic culture

51%

Duskova J., Tishchenko G., Ponomareva E., Simunek J., Koppova I., Skalova T., Stepankova A., Hasek J., Dohnalek J.

Acta Biochimica Polonica

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2011

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tom 58

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nr 2

The object of this study are chitinolytic enzymes produced by bacterium Clostridium paraputrificum J4 isolated from the gastrointestinal tract of a healthy human. In particular, we focus on the development of purification protocols, determination of properties of the enzymes and their activity profiles. The process of bacteria cultivation and isolation of chitinolytic complex of enzymes showing specific activities of endo-, exo-chitinase and N-acetyl-β-glucosaminidase was optimized. A range of various purification procedures were used such as ultrafiltration, precipitation, chromatographic separations (ion-exchange, size exclusion, chromatofocusing) in altered combinations. The optimal purification protocol comprises two or three steps. Individual samples were analyzed by SDS/PAGE electrophoresis and after renaturation their activity could be detected using zymograms. Mass spectroscopy peptide fragment analysis and MALDI analysis of the purest samples indicate presence of endochitinase B (molecular mass about 85 kDa) and of 60-kDa endo- and exochitinases.

Ograniczanie wyników

3 Herba Polonica

1 Acta Biochimica Polonica

4 Duskova J.

3 Dusek J.

1 Dohnalek J.

1 Hasek J.

1 Jahodar L.

1 Koppova I.

1 Ponomareva E.

1 Simunek J.

1 Skalova T.

1 Stepankova A.

1 Tishchenko G.

1 Tumova L.

1 2011

1 1999

2 1995

Leuzea carthamoides DC. in vitro

Zur biotransformation von Hydrochinon zu Arbutin in den in Vitro-Kulturen

Possibilities of using medicinal plants from the area exposed to lead

Chitinolytic enzymes from bacterium inhabiting human gastrointestinal tract - critical parameters of protein isolation from anaerobic culture