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As China has committed to the international community to effectively control CO₂ emissions, it is necessary for the regions of China to launch an in-depth analysis about energy use and CO₂ emission efficiency. In this paper, each province of China will be regarded as an independent decision-making unit. After judging their return to scale state by the traditional DEA theoretical model, we use the SBM and RE/CE models, which respectively solve the problems of slack variables, and the traditional model does not reflect factors such as price, which is truly existing. Then we get a more comprehensive efficiency that reveals Chinese energy use and the CO₂ emissions situation. From the empirical study of 30 regions in China, we know that the southern region of China has the most efficient score while northeastern China has poor performance. Price factor has a significant influence on energy use and CO₂ emissions efficient score of some provinces. Our study shows that northern and northeastern China should make more of an effort on energy consumption reduction in order to improve the efficiency score. Instead, northeastern and central China should pay more attention improving energy conversion technology in order to increase their efficiency.
With rapid urbanization and the rise of climate change awareness, policy makers and urban planners are seeking methods and schemes that consider the urban heat island (UHI) effect. In order to investigateto what extent a homogeneous urban fabric leads to a specific thermal pattern, a local climate zone (LCZ) scheme was utilized and classification has been applied in the urban areas of Chongqing. Urban indicators were calculated to match LCZ types of measurement points. Using a mobile measurement approach, we analyzed screen-height air temperature distribution inside local climate zones in both spring and summer. An apparent seasonal difference inside LCZs was found, with air temperature amplitude about 0.8ºC in spring and 0.9ºC in summer. The average air temperature difference in LCZ 1 was 2.9ºC between the two seasons, while in LCZ H this difference was as high as 3.5ºC. The reasons why LCZ H had relatively high air temperature were explained and further investigation of UHI using LCZ scheme was proposed.
Radiotherapy and chemotherapeutic agents that damage DNA are the current major non-surgical means of treating cancer. However, many patients develop resistances to chemotherapy drugs in their later lives. The PI3K and Ras signaling pathways are deregulated in most cancers, so molecularly targeting PI3K-Akt or Ras-MAPK signaling sensitizes many cancer types to radiotherapy and chemotherapy, but the underlying molecular mechanisms have yet to be determined. During the multi-step processes of tumorigenesis, cancer cells gain the capability to disrupt the cell cycle checkpoint and increase the activity of CDK4/6 by disrupting the PI3K, Ras, p53, and Rb signaling circuits. Recent advances have demonstrated that PI3K-Akt-mTOR signaling controls FANCD2 and ribonucleotide reductase (RNR). FANCD2 plays an important role in the resistance of cells to DNA damage agents and the activation of DNA damage checkpoints, while RNR is critical for the completion of DNA replication and repair in response to DNA damage and replication stress. Regulation of FANCD2 and RNR suggests that cancer cells depend on PI3K-Akt-mTOR signaling for survival in response to DNA damage, indicating that the PI3K-AktmTOR pathway promotes resistance to chemotherapy and radiotherapy by enhancing DNA damage repair.
Upregulation of histone acetylation plays a critical role in the dysregulation of transcription. It alters the structure of chromatin, which leads to the onset of cancer. Histone deacetylase inhibitors may therefore be a promising way to limit cancer progression. In this study, we examined the effects of droxinostat on the growth of HT-29 colon cancer cells. Our results show that droxinostat effectively inhibited cell growth and colony-forming ability by inducing cellular apoptosis and ROS production in HT-29 cells. Notably, the apoptotic inhibitor Z-VAD-FMK significantly decreased the levels of cellular apoptosis and the antioxidant γ-tocotrienol (GT3) significantly decreased ROS production induced by droxinostat treatment. Z-VAD-FMK and GT3 also partially reversed the negative growth effects of droxinstat on HT-29 cells. GT3 treatment decreased cellular apoptosis and increased colony-forming ability upon droxinostat administration. Z-VAD-FMK treatment also partially decreased droxinostat-induced ROS production. Our findings suggest that the effects of droxinostat on colon cancer cells are mediated by the induction of oxidative stress and apoptotic cell death.
Even though solid backfill mining can address some environmental issues of coal mining (like surface subsidence and gangue storage), on the other hand the long exposure of gangue to rain or other surficial water will also result in the leaching of heavy metal ions, which in turn will pose the threat of potential pollution to groundwater. In response to resolving the above-mentioned problem, this paper simulated the concentration distribution and migration principle of Cu²⁺ in various soils by applying COMSOL Multiphysics software, and the results show that the diffusion distance of Cu²⁺ in different soils are ranked as clay, silty clay, loess, and sandy soil. In addition, the concentration will decrease with the increase of depth and the Darcy velocity contours of Cu²⁺ distribute radially and symmetrically in the transverse direction. The velocity gradient of Darcy velocity in different soils is clay soil > Silty clay soil > loess > sandy soil.
With the development of automation in ports, the video surveillance systems with automated human detection begun to be applied in open-air handling operation areas for safety and security. The accuracy of traditional human detection based on the video camera is not high enough to meet the requirements of operation surveillance. One of the key reasons is that Histograms of Oriented Gradients (HOG) features of the human body will show great different between front & back standing (F&B) and side standing (Side) human body. Therefore, the final training for classifier will only gain a few useful specific features which have contribution to classification and are insufficient to support effective classification, while using the HOG features directly extracted by the samples from different human postures. This paper proposes a two-stage classification method to improve the accuracy of human detection. In the first stage, during preprocessing classification, images is mainly divided into possible F&B human body and not F&B human body, and then they were put into the second-stage classification among side human and non-human recognition. The experimental results in Tianjin port show that the two-stage classifier can improve the classification accuracy of human detection obviously
Difference in isozymes and activities of peroxidase (POD) and superoxide dismutase (SOD) in two barley (Hordeum vulgare L.) genotypes differing in salt tolerance (Gebeina, tolerant; Quzhou, sensitive) was investigated using a hydroponic experiment. The activities of both enzymes were significantly increased when the plants of the two barley genotypes were exposed to salt stress, with salt-tolerant genotype being generally higher than the sensitive one. The variation in the POD and SOD isozymes was dependent on barley genotype, salt level and exposure time. When the plants were exposed to salt stress for 10 days, two new POD isozymes were found, Rm0.26 (Rm, relative mobility of enzyme to dye) in Gebeina and Rm0.45 in Quzhou. Both isozymes disappeared after 20 days of salt stress, but Rm0.26 appeared again 30 days after the stress. Two new SOD isozymes of Rm0.19 and Rm0.46 were found in Gebeina when exposed to NaCl for 10 days, but only Rm0.46 in Quzhou. As the time of salt stress extended, more new SOD isozymes were detected, Rm0.35 in both genotypes in all different salt treatments and Rm0.48 in Gebeina under 200 mM NaCl stress. At 30 days after the stress, all the new SOD isozymes disappeared except for Rm0.48 in Gebeina under 200 mM NaCl stress. The results suggest that the increased POD and SOD activities could be partly due to the formation of some new isozymes and the tolerant variety had better ability to form new isozymes to overcome salt stress.
Plant growth, photosynthetic parameters, chloroplast ultrastructure, and the ascorbate-glutathione cycle system in chloroplasts of self-grafted and rootstock-grafted cucumber leaves were investigated. Grafted plants were grown hydroponically and were exposed to 0, 50, and 100 mM NaCl concentrations for 10 days. Under NaCl stress, the hydrogen peroxide (H₂O₂) content in cucumber chloroplasts increased, the chloroplast ultrastructure was damaged, and the gas stomatal conductance, intercellular CO₂ concentration, as well as shoot dry weight, plant height, stem diameter, leaf area, and leaf relative water content were inhibited, whereas these changes were less severe in rootstock-grafted plants. The activities of ascorbate peroxidase (APX; EC 1.11.1.11), glutathione reductase (GR; EC 1.6.4.2), and dehydroascorbate reductase (DHAR EC 1.8.5.1) were higher in the chloroplasts of rootstock-grafted plants compared with those of self-grafted plants under 50 and 100 mM NaCl. Similar trends were shown in leaf net CO₂ assimilation rate and transpiration rate, as well as reduced glutathione content under 100 mM NaCl. Results suggest that rootstock grafting enhances the H₂O₂-scavenging capacity of the ascorbate–glutathione cycle in cucumber chloroplasts under NaCl stress, thereby protecting the chloroplast structure and improving the photosynthetic performance of cucumber leaves. As a result, cucumber growth is promoted.
Neuromedin S (NMS), a 36-amino acid neuropeptide, has been found to be involved in the regulation of the endocrine activity. It has been also detected in immune tissues in mammals, what suggests that NMS may play an important role in the regulation of immune response. The aim of this study was to demonstrate the presence of NMS receptor 1 (NMU1R) and effect of NMS in pig splenic lymphocytes (SPLs) and pulmonary alveolar macrophages (PAMs). The presence of NMU1R in pig SPLs and PAMs was respectively confirmed by reverse transcription-polymerase chain reaction (RT-PCR), western blot analysis and immunocytochemical methods. Furthermore, SPL proliferation was analyzed using the 3-(4,5)-dimethyl-thiahiazo-(-2-yl)-3,5-di-phenytetrazoliumromide (MTT) method. Additionally, the secretion of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α) in PAMs was all measured by enzyme-linked immunosorbent assay (ELISA) kits. In the present study, the results of RT-PCR and western blot analysis revealed that NMU1R mRNA and protein were both expressed in pig SPLs and PAMs, and the immunocytochemical investigations further revealed that the positive signal of NMU1R immunoreactivity was observed in plasma membranes of both SPLs and PAMs. In the in vitro study, we found that at concentrations of 0.001-1000 nM NMS alone or combined with lipopolysaccharide or phytohemagglutinin significantly increased SPL proliferation. Application of ELISA method showed that NMS could induce the secretion of the pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in PAMs. These results suggest that NMS can act as a potently positive pro-inflammatory factor and immunomodulatory agent that affects the immune response of immune cells by combining with its receptor NMU1R.
Background. Half-smooth tongue sole, Cynoglossus semilaevis Günther, 1873, a marine teleost, is an important aquaculture species of great economic value. In recent years, its farm production increase coincided, however, with the number of reported cases of bacterial diseases. Further understanding of its immune response to bacterial pathogens can provide more information on pathogenesis and how to prevent disease using immune-related strategy. Peptidoglycan (PGN) recognition proteins (PGRPs) play important roles in the innate immunity against bacterial infection. In the presently reported study, a long type PGRP in half-smooth tongue sole (csPGRP-L) was cloned, and its sequence features, PGN binding ability, and mRNA expressions in different tissues after bacterial infection were also analyzed. Materials and Methods. The full length of csPGRP-L cDNA was obtained by RT-PCR and RACE-PCR method, and its sequence features were analyzed by multiple sequence alignment and phylogenetic tree. Meanwhile, its 3-D structure and PGN binding ability were analyzed by comparative modelling and molecular docking methods. Furthermore, the expressions of csPGRP-L in different tissues of healthy fish and fish infected with Streptococcus dysgalactiae were examined using quantitative real-time PCR method. Results. The full length of csPGRP-L cDNA was 1509 bp (GenBank accession No. HQ909441), with a 1446 bp of open reading frame (ORF) encoding 481 amino acids (aa), which possessed several conserved PGRP family features, e.g., a typical PGRP domain at its C-terminal, 3-D structure. Molecular docking showed that the csPGRP-L also possessed the PGN-binding ability. csPGRP-L was constitutive expressed in all the selected tissues from healthy fish and following S. dysgalactiae infection its expression was up-regulated in a tissue-specific expression pattern. Conclusion. The gene we cloned was exactly the homologue of vertebrates’ long type PGRP in half-smooth tongue sole which was confirmed by several analyses and the up-regulation of csPGRP-L after bacterial infection suggest that csPGRP-L plays important role in antibacterial and anti-infective action.
The aim of the study was to investigate whether the auditory cortex supports working memory, the short-term storage of information for goal-directed behavior. In order to achieve this, we carried out a series of experiments with non-human primates (Macaca fascicularis) and humans, who performed various tasks on two-sound sequences. We measured spiking activity of individual neurons and local field potentials in the auditory cortex of the monkeys, and the activity of neural populations in the auditory cortex of humans by means of magnetoencephalography (MEG). The experiments were designed in such a way that they enabled us to identify memory-related activity and disentangle it from activity related to other confounding factors, for example from activity associated with motor preparation. We found persistent auditory cortical activity in the silent period between the two sounds that was clearly related to the short-term storage of task-relevant information. Collectively, we found direct support in both species for the idea that temporary storage of information recruits the sensory areas which initially processthe information. In our effortto understand the processes underlying auditory sensory and working memory, we are using the computational model of signal processing in the auditory cortex developed by May et al. In this model, the serial and parallel structure of auditory cortex is combined with short-term plasticity, and the dynamical units represent the mean spiking rates of local, excitatory and inhibitory neural populations. We show that the model is able to account for the observed phenomena related to sensory and working memory. In so doing, it bridges the gap between single- and multiunit measurements in monkeys and MEG experiments in humans. FINANCIAL SUPPORT: This research was supported by a LIN Special Project, and by the Deutsche Forschungsgemeinschaft (He 1721/10-1, He 1721/10-2, SFB-TRR31/ A4). We also acknowledge the support by an Alexander von Humboldt Polish Honorary Research Fellowship by the Foundation for Polish Science.
Competition among trees is a fundamental interaction process within plant community, which is the theoretical basis of thinning. Plant competitive intensity is generally measured using a competition index (CI) that can be classified into two major categories: distance-independent and distance-dependent. The current study used Cunninghamia lanceolata (Lamb.) Hook as the test subject and used Hegyi's CI (distance-dependent), to quantify individual CI and their relationship with tree diameter at breast height (DBH). Five different criteria were used to select potential competitors for the calculation of CI. Seven basic linear and nonlinear mathematical functions were used to test and quantify the relationships between DBH of the target tree and the individual CI. Results showed that individual CI was negatively correlated with target tree DBH: as DBH increased, competition intensity weakened. The adjusted R² with five different criteria of selection competitors simulated by seven functions ranged from 0.30 to 0.82. Considering the root mean square error (RMSE), P-value, and adjusted-R², our results suggested that the best model to simulate the relationship between individual CI and focal tree DBH was power function (CI = 43.98 × DBH⁻¹‧⁰⁸, adjusted R² = 0.81) and with the Voronoi diagram method as the criteria for selecting competitors. These results can demonstrate a clearer understanding of the spatial structure of forests, and can be used to guide the selection of thinning trees in the process of thinning practice.
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