Ograniczanie wyników

Czasopisma help
Autorzy help
Lata help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 53

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
Edwardsiella ictaluri is a Gram-negative bacterium and the causative agent of enteric septicemia of catfish. In this study, we examined the expression and function of the LuxS from a pathogenic E. ictaluri strain, J901. J901 was found to produce autoinducer 2 (AI-2) activity that maximized at mid-logarithmic phase and was enhanced by glucose and repressed by high temperature. Consistently, a luxS gene (luxSEi) was identified in J901, whose expression was regulated by cell density, glucose, and temperature in a manner similar to that observed with AI-2 activity. Further analysis showed that LuxSEi is a biologically active AI-2 synthase that was able to complement the luxS-defective phenotype of Escherichia coli DH5. To examine the functional importance of LuxSEi, a genetically modified variant of J901, J901Ri, was constructed, in which luxSEi expression was blocked by RNA interference. Compared to the wild type, J901Ri was (i) reduced in AI-2 activity to a level of 59% of that of the wild type; (ii) impaired in both planktonic and biofilm growth; (iii) significantly attenuated in the ability to infect cultured fish cells and to cause mortality in infected fish; (iv) unable to induce the expression of certain virulence-associated genes. Addition of exogenous AI-2 failed to rescue the growth defect of J901Ri as free-living cells but restored biofilm production and the expression of virulence genes to levels comparable to those of the wild type. Taken together, these results indicate that LuxSEi is a functional AI-2 synthase that is required for optimal cellular growth and host infection.
We suggest to transfer the empirical downscaling methodology, which was developed mostly for atmospheric dynamics and impacts, to regional ocean problems. The major problem for doing so is the availability of decades-long and homogeneous and spatially detailed data sets. We have examined the performance of the STORM multidecadal simulation, which was run on a 0.18 grid and forced with 1950—2010 NCEP re-analyses, in the South China Sea and found the data suitable. For demonstration we build with this STORM-data downscaling model for the regional throughflow. The STORM data is compared with AVISO satellite observations and the ocean re-analysis dataset C-GLORS. We find the seasonal patterns and the inter-annual variability of sea surface height anomaly in both the C-GLORS data and the STORM simulation consistent with the AVISO- satellite data. Also the strong westward intensification and the seasonal patterns of South China Sea circulation steered by the monsoon have been presented well. As an important indicator of vertical movement, the sea surface temperature distribution maps are also very close, especially for the narrow upwelling region in summer. We conclude that the output of the STORM simulation is realistically capturing both the large-scale as well as the small-scale dynamical features in the South China Sea.
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Mixed noble gas effect on cut green peppers

81%
Increasing attempts at using gas which leads to hydrate formation as a preservative tool in fresh-cut fruits and vegetables have been reported. In this study, changes in some physical and biochemical properties of fresh-cut green peppers under compressed noble gas treatments were examined. Mixed argonkrypton and argon treatments were performed before cold storage at 5°C for 15 days. Mass loss and cell membrane permeability were found to be the lowest in mixed argon-krypton samples. Besides, a lowerCO2 concentration and vitamin C loss were detected in gastreated samples compared to untreated samples (control). While the total phenol degradation was moderately reduced, the effect of the treatment on polyphenoloxidase activity was better at the beginning of the storage period. The minimum changes in quality observed in cut peppers resulted from both mixed and gas treatment alone.
Based on the energy consumption data of power industry in the Beijing-Tianjin-Hebei region from 1995 to 2014, our paper first estimated CO₂ emissions using the IPCC carbon accounting methods. Then, starting from the perspective of the power industry chain – including power generation, transmission, and final consumption – we established the hierarchical LMDI decomposition model; decomposed driving factors of CO₂ emissions into effects of fuel mix; the coal consumption rate; power generation structure; the ratio of power generation to consumption, transmission, and distribution losses; production sectors’ electricity intensity; industrial structure; household electricity intensity; economic scale; and population size. Results show that: 1. During 1995-2014, CO₂ emissions of power industry in the Beijing-Tianjin-Hebei region developed in fluctuation and showed a rising trend in general, with annual average growth rate of 5.93%. 2. The factors that drive the growth of CO₂ emissions from the power industry in the Beijing-Tianjin-Hebei region are, in order, economic scale, population size, transmission and distribution losses, and industrial structure, with a contribution rate of 150.70%, 20.80%, 8.86%, and 8.83%. The factors that drive CO₂ emissions reduction are production sectors’ electricity intensity, the coal consumption rate, the ratio of electricity generation and consumption, household electricity intensity, power generation structure, and fuel mix, with a contribution rate of -45.97%, -22.38%, -19.41%, -0.62%, -0.49%, and -0.32%, respectively
Two cultivars of Jerusalem artichoke (Helianthus tuberosus L.) differing in genotype, Red skin (cv. R., salt-tolerant but low-yield) and White skin (cv. W., salt-sensitive but high-yield), were used to investigate malondialdehyde (MDA) content and antioxidant enzyme activity changes in their roots under a hydroponic culture system with 250 mM NaCl. The results showed that MDA contents in roots of the two genotypes increased, but MDA content of cv. R. was higher than that of cv. W. Changes in all antioxidant enzymes in roots of both varieties exhibited a similar trend, namely increased initially and then decreased. However, there were still some differences existing between the two cultivars. In other words, activities of the other two antioxidant enzymes except catalase (CAT) and peroxidase (POD) in roots of cv. R. were less than controls at 48 h, while all others except ascorbate peroxidase (APX) in roots of cv. W. were greater than controls. The peak of superoxide dismutase (SOD) activity of cv. W. was observed to appear earlier than that of cv. R. CAT activity of cv. W. was significantly greater than the value of cv. R. and the latter showed a moderate trend. POD activity of cv. R. obtained the maximum at 6 h, whereas the peak of cv. W. displayed at 24 h. APX activity of cv. R. declined more than that of cv. W. These results suggested that there was a lower efficiency of scavenging reactive oxygen species (ROS) in cv. R. roots. Concomitantly, salt stress caused more severe damage to roots of cv. R. Antioxidant enzymes in roots were inadequate to elucidate salt-tolerance mechanisms of the whole plant.
Four new species of earthworm species coEected from various parts of Hainan Island were found. The species belong to Amynthas and Metaphire and are characterized by having two pairs of spermathecae in 7/8-8/9. They are described here as Amynthas tlexuosus sp. nov., A. lucidus sp. nov., A. haikouensis sp. nov. in the aeruhinosus- group of species of the genus Amynthas, and Metaphire fortuita sp. nov. in the insularui-group of species of the genus Metaphire. Their anatomical and morphological characteristics are compared to similar species from Hainan Island, China mainland and South-East Asia.
Branch bending has been practiced for decades as an effective means of inducing floral buds in ‘Fuji’ apple in China. Endogenous hormones play an important role in apple flowering. The main purpose of this study is to investigate the hormone concentrations (ABA, ZR, IAA and GA) in spurs, medium and long shoot terminals of ‘Fuji’ and ‘Gala’ apple trees subjected to bending of different angles (70°, 90° or 110°). In both cultivars, the proportion of floral buds and spurs increased, but that of medium and long shoots decreased with increasing bending angles. TheABA and ZR concentrations in shoot terminals increased, but the IAA and GA concentrations decreased as the bending angles increased from 70° to 110°. The ZR concentration increased monotonically in spurs apexes, but in medium and long shoot terminals it increased from a minimum on May 10 to a maximum on June 20 for ‘Fuji’ apple trees or on July 20 for ‘Gala’ apple trees, and then decreased thereafter. It was also observed that the ‘Gala' apple trees had a higher ABA concentration but a lower IAA concentration in shoot terminals than the ‘Fuji’ apple trees. The ZR concentration in spurs apexes of ‘Gala’ apple trees was higher before July 1, and the GA concentration was higher before June 20, but lower after July 20 than the ‘Fuji’. The (IAA + GA + ZR)/ABA ratio of spurs apexes in ‘Fuji’ apple trees decreased with increasing bending angles during the whole growth season, with a significant difference between those bent at 110° and those bent at 70°. There was no significant difference for the ‘Gala’ apple trees. The (IAA + GA + ZR)/ABA ratio peaked on June 20, and it was higher in ‘Fuji’ than in ‘Gala’ apple trees throughout the study period.
Using the ARDL test and Granger approaches, this paper investigates long-term co-integration and causal relationships among nuclear, renewables, GDP and CO2 emissions in China, US, France and Japan. The findings reveal that the long-term coefficient of nuclear energy is smaller than renewable energy, indicating a relative advantage of nuclear power. However, the emission reduction effect of nuclear and renewables in China was not as much as compared to other nations. Moreover, causality from economic growth to nuclear or renewable energy existed in all countries, whereas no causality from nuclear energy to economic growth existed except in Japan. Therefore, giving priority to energy conservation and energy mix optimization is not likely to hurdle economic growth in China. However, greenness of the overall energy production process, augmentation of energy efficiency, technological progress in energy storage and internet, sustainable development of resource-society, and nuclear safety issues should be given priority in order to contribute to low-carbon growth in China.
To solve the nonlinear control problems of the unknown time-varying environmental disturbances and parametric uncertainties for ship course-keeping control, this paper presents an adaptive self-regulation PID (APID) scheme which can ensure the boundedness of all signals in the ship course-keeping control system by using the Lyapunov direct method. Compared with the traditional PID control scheme, the APID control scheme not only is independent of the model parameters and the unknown input, but also can regulate the gain of PID adaptively and resist time-varying disturbances well. Simulation results illustrate the effectiveness and the robustness of the proposed control scheme
The study of the flow characteristics of the solid-fluid two phase flow in the cutter suction dredger is very important for exploring the slurry formation mechanism and optimizing the operational parameters. In this study, standard k-ε model and Multiple Reference Frame are applied to numerically simulate flow field in and around the cutting system, then with the steady convergent result of the simulation as the initial condition, Discrete Phase Mode is used to solve the particle motion equation by fully coupling the continuous phase and the particles . The influence of suction flow velocity and cutter’s rotating speed on particles suction are analyzed, and effectively suctioned particles numbers are also quantitatively studied. The simulation result shows that the DPM model is able to simulate the movement of particles in and around the cutter suction dredger’s cutting system, in the fluid flow filed velocity vector and pressure distribution on different planes show different characteristics, and under higher suction velocity and lower cutter rotating speed more particles are suctioned into the suction inlet. The results can help better understand flow characteristics of solid-fluid 2-phase-flow of cutter suction dredger’s cutting system, and provide theoretical support for relative system design and operational parameters optimization
To analyze the physiological mechanisms underlying the increased tolerance to drought and high temperature stress combination by overproduction of glycinebetaine (GB) in wheat, a transgenic wheat line T6 and its wild-type (WT) Shi4185 were used. The transgenic line was generated by introducing a gene encoding betaine aldehyde dehydrogenase (BADH) into a wheat line Shi4185. The gene was cloned from Garden Orache (Atriplex hortensis L.). Wheat plants were exposed to drought (withholding irrigation), high temperature stress (40°C), and their combination at the flowering stage. Analyses of oxygen-evolving activity and photosystem II (PSII) photochemistry, modulated chlorophyll fluorescence, rapid fluorescence induction kinetics, and the polyphasic fluorescence transients (OJIP) were used to evaluate PSII photochemistry in wheat plants. The results suggest that the PSII in transgenic plants showed higher resistance than that in wild-type plants under the stresses studied here, this increased tolerance was associated with an improvement in stability of the oxygen-evolving complex and the reaction center of PSII; streptomycin treatment can impair the protective effect of overaccumulated GB on PSII. The overaccumulated GB may protect the PSII complex from damage through accelerating D1 protein turnover to alleviate photodamage. The results also suggest that the PSII under combined high temperature and drought stress shows higher tolerance than under high temperature stress alone in both transgenic and wild-type plants.
We characterized 716 sites (including 22 beds, 60 foraging and 604 movement sites) used by red deerCervus elaphus xanthopygus Milne-Edwards, 1867 in the Wandashan Mountains, northeastern China in the winter period. We used covariates for vegetation, topography, disturbances by other ungulates, and disturbance by humans to develop movement, forage and bed site resource-selection models. We used an information-theoretic approach to select the top 5 models for movement, forage and bed site occurrences respectively. The three most parsimonious autologistic models were good predictors of movement, forage and bed occurrence of this species. Vegetation covariates were important components of all models. We recorded avoidance responses by the behaviours of species to each disturbance type (ie, villages, forest roads and abandoned roads); bed site occurrences were most sensitive to all human disturbances for their lowest odds encountered. Across all parsimonious models, villages have the largest negative effect on movement, foraging and bed occurrences. Movement and bedding behaviours were also affected by other ungulate disturbances. Altitude had slightly negative effect on movement and foraging behaviours. Ridges, topographic aspect and shrub stands were all correlated with bed-site selection. Although different behaviours may be associated with different microhabitat features, behaviours of red deer responded similarly to the same human disturbance on a broad scale. Based on the observations above, we believe that red deer are avoiding human-altered habitat for movement, bedding and foraging because of disturbances during the critical late winter period. Furthermore, various behavioural resource selection models and corresponding graphs of important habitat disturbances can be used to guide and evaluate future development proposals.
Several lines of evidence suggest that nitric oxide (NO) and hydrogen peroxide (H₂O₂) are important signal molecules involved in plant development and other physiological processes. Marigold (Tagetes erecta L. ‘Marvel’) was used to understand the role and relationship of NO and H₂O₂ in adventitious root development of plants. The results showed that the effects of H₂O₂ or NO on adventitious root organogenesis of explants were dose dependent, with maximal biological responses at 200 µM H₂O₂ or 50 µM NO donor sodium nitroprusside (SNP). The results also indicated the importance of both putative NO synthase (NOS)-like and nitrate reductase (NR) enzymes, which might be responsible for the production of NO in explants during rooting. Additionally, guanosine 3', 5' -cyclic monophosphate (cGMP) was involved in NO-induced root formation of marigold, but it was not involved in H₂O₂- mediated rooting process. The root number and length of explants treated with NO and H₂O₂ simultaneously were significantly higher than those of explants treated with H₂O₂ or NO alone. Moreover, NO treatments enhanced endogenous H₂O₂ levels in hypocotyls. Together, these results indicate that NO and H₂O₂ play crucial roles in the adventitious root development of marigold explants both synergistically and independently.
Effects of ultrasound, high pressure argon, and treatments comprising their combinations on physicochemical and microbiological characteristics of white mushrooms were studied during 9 days of storage at 4°C. High pressure argon treatments were relatively effective in retaining firmness and were found to maintain the cell integrity. White mushrooms firmness after 9 days of storage was increased from 2.79 N for untreated mushrooms up to 3.01, 3.24, 3.58 N for ultrasound, treatments comprising ultrasound and high pressure argon, high pressure argon, respectively. Similarly, the loss of water, ascorbic acid and total soluble solid in fresh mushroom was also greatly reduced by the high pressure argon treatment. The ultrasound treatment followed by treatments comprising ultrasound and high pressure argon and high pressure argon, respectively exhibited a pronounced effect on retarding browning and in delaying mesophilic and psychrotrophic bacteria, yeasts and moulds growth in white mushroom, compared to the control during 9 days of cold storage. Treatments comprising ultrasound and high pressure argon treatment delayed pseudomonas growth, implying that it could extend shelf life of white mushrooms to 9 days at 4°C.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.