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1

Bialko IHF jako regulator metabolizmu Escherichia coli

100%
Sirko A.
Postępy Biochemii
|
1998
|
tom 44
|
nr 4
345-354
2

Metabolizm siarki w roślinach i jego regulacja

63%
Moniuszko G., Sirko A.
Postępy Biochemii
|
2008
|
tom 54
|
nr 4
3

Recent advances in understanding plant response to sulfur-deficiency stress

63%
Lewandowska M., Sirko A.
Acta Biochimica Polonica
|
2008
|
tom 55
|
nr 3
457-471
Sulfur is an essential macronutrient for all living organisms. Plants are able to assimilate inorganic sulfur and incorporate it into organic compounds, while animals rely entirely on organic sources of sulfur. In the last decades sulfate availability in soils has become the major limiting factor for plant production in many countries due to significant reduction of anthropogenic sulfur emission forced by introducing stringent environmental legislation. The sulfur flux after transferring plants from optimal conditions to sulfur deficiency is regulated on multiple levels including transcription, translation and activity of enzymes needed for sulfate assimilation and synthesis of sulfur-containing metabolites. Most of these regulatory steps are not yet fully characterized. Plant responses to sulfur limitation are complex and can be divided into phases depending on the degree of sulfur shortage. The initial responses are limited to adaptations within sulfur metabolic pathway, while multiple metabolic pathways and developmental process are affected when sulfur shortage becomes more severe. The major aim of this work is a comprehensive review of recent progress in understanding the regulation of plant adaptations to sulfur deficit.
4

Identification and characteristics of tobacco clones encoding an organellar isoform of serine acetyltransferase and a cytosolic isoform of cysteine synthase

63%
Liszewska F., Sirko A.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
5

Plant ureases: roles and regulation

63%
Sirko A., Brodzik R.
Acta Biochimica Polonica
|
2000
|
tom 47
|
nr 4
Both urea and urease were subjects of early scientific investigations. Urea was the first organic molecule to be synthesized and jack bean urease was the first enzyme ever to be crystallized. About 50 years later it was shown to be the first nickel metalloenzyme. Since then, nickel-dependent ureases have been isolated from many bacteria, fungi and higher plants. They have similar structures and mechanisms of catalysis. A urease apoenzyme needs to be activated. This process requires participation of several accessory proteins that incorporate nickel into the urease forming catalytic site. In this review, ureases from various organisms are briefly described and the similarities of their structures discussed. Moreover, the significance of urea recycling in plants is explained and recent literature data about the function and activation of plant ureases are presented.
6

Wykorzystanie systemów roślinnych do produkcji rekombinowanych cytokin

51%
Gora-Sochacka A., Redkiewicz P., Napiorkowska B., Sirko A.
Postępy Biochemii
|
2009
|
tom 55
|
nr 1
7

Influenza prevention and treatment by passive immunization

51%
Kalenik B., Sawicka R., Gora-Sochacka A., Sirko A.
Acta Biochimica Polonica
|
2014
|
tom 61
|
nr 3
8

DNA vaccines against influenza

51%
Stachyra A., Gora-Sochacka A., Sirko A.
Acta Biochimica Polonica
|
2014
|
tom 61
|
nr 3
9

Identification and mapping of the sulphate permease promoter region in Escherichia coli

51%
Hryniewicz M., Sirko A., Hulanicka D.
Acta Biochimica Polonica
|
1989
|
tom 36
|
nr 3-4
10

Metabolizm siarki w roslinach wyzszych.

51%
Sirko A., Blaszczyk A., Liszewska F.
Działalność Naukowa PAN. Wybrane Zagadnienia
|
2002
|
tom 13
41-42
11

Plant expression systems for production of hemagglutinin as a vaccine against influenza virus

51%
Redkiewicz P., Sirko A., Kamel K. A., Gora-Sochacka A.
Acta Biochimica Polonica
|
2014
|
tom 61
|
nr 3
12

Characterization of the Escherichia coli gene encoding a new member of the short-chain dehydrogenase/reductase [SDR] family

51%
Sirko A., Weglenska A., Hryniewicz M., Hulanicka D. M.
Acta Biochimica Polonica
|
1997
|
tom 44
|
nr 1
The nucleotide sequence of a chromosomal DNA fragment located upstream from the cysPTWAM operon of Escherichia coli was established. Sequence analysis indicates the presence of an open reading frame which has been designated ucpA (upstream cysE). The potential protein product exhibits strong sequence homology to the members of a large protein family, short-chain dehydrogenases/reductases. Involvement of Crp, FruR and IHF in the regula­tion of ucpA transcription in vivo was demonstrated.
13

Production of urease from Helicobacter pylori in transgenic tobacco plants

51%
Brodzik R., Koprowski H., Yusibov V., Sirko A.
Cellular and Molecular Biology Letters
|
2000
|
tom 05
|
nr 3
H. pylori ureA and ureB genes encoding both subunits of urease were expressed transgenically in a low-alkaloid line of tobacco (LA Burley 21). Analysis of transgene expression at both the mRNA and protein levels revealed a significant increase (up to 8-fold) in ammonia concentration correlated with an amount of UreB protein detected in the leaves, and an increase (up to 2-fold) in urease activity in transformants as compared to control plants.
14

Isolation of Nicotiana plumbaginifolia cDNAs encoding isoforms of serine acetyltransferase and O-acetylserine [thiol] lyase in a yeast two-hybrid system with Escherichia coli cysE and cysK genes as baits

51%
Liszewska F., Gaganidze D., Sirko A.
Acta Biochimica Polonica
|
2005
|
tom 52
|
nr 1
We applied the yeast two-hybrid system for screening of a cDNA library of Nicotiana plumbaginifolia for clones encoding plant proteins interacting with two proteins of Escherichia coli: serine acetyltransferase (SAT, the product of cysE gene) and O-acetylserine (thiol)lyase A, also termed cysteine synthase (OASTL-A, the product of cysK gene). Two plant cDNA clones were identified when using the cysE gene as a bait. These clones encode a probable cytosolic isoform of OASTL and an organellar isoform of SAT, respectively, as indicated by evolutionary trees. The second clone, encoding SAT, was identified independently also as a “prey” when using cysK as a bait. Our results reveal the possibility of applying the two-hybrid system for cloning of plant cDNAs encoding enzymes of the cysteine synthase complex in the two-hybrid system. Additionally, using genome walking sequences located upstream of the sat1 cDNA were identified. Subsequently, in silico analyses were performed aiming towards identification of the potential signal peptide and possible location of the deduced mature protein encoded by sat1.
15

Modification of non-protein thiols contents in transgenic tobacco plants producing bacterial enzymes of cysteine biosynthesis pathway

51%
Liszewska F., Blaszczyk A., Sirko A.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 3
Conditions of achieving the maximal accumulation of sulfhydryl metabolites in the leaves of tobacco were explored. Simultaneous production of bacterial O-acetylserine (thiol)-lyase and serine acetyltransferase resulted in the increased thiols contents as compared to single transformants and controls. However, leaf discs feeding experi­ments differently affected thiols concentration in different plant groups and sug­gested that the most promising strategy to obtain plants with a high level of non-protein thiol-containing compounds might be sulfate feeding to plants overpro­ducing serine acetyltransferase.
16

Identifications and preliminary characteristics of several novel tobacco genes regulated by sulphur deficiency stress

51%
Lewandowska M., Wawrzynska A., Sirko A.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
17

Cysteine regulon of Enterobacteriaceae

45%
Zatyka M., Sirko A., Jagura-Burdzy G., Hryniewicz M., Hulanicka D.
Postępy Biochemii
|
1992
|
tom 38
|
nr 2
46-48
18

Antibody response to DNA vaccine against H5N1 avian influenza virus in broilers immunized according to three schedules

45%
Stachyra A., Gora-Sochacka A., Zagorski-Ostoja W., Krol E., Sirko A.
Acta Biochimica Polonica
|
2014
|
tom 61
|
nr 3
19

Polyadenylation and decay of 26S rRNA as part of Nicotiana tabacum response to cadmium

45%
Lewandowska M., Borcz B., Kaminska J., Wawrzynski A., Sirko A.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr 4
747-755
In contrast to mRNAs, ribosomal RNAs are generally not considered to be polyadenylated. Only a few recent reports describe non-abundant polyadenylated rRNA-related transcripts that have been detected and characterized in yeast and in human cells. Here we depict the phenomenon of 26S rRNA polyadenylation and degradation that was observed in shoots of Nicotiana tabaccum plants grown in the presence of cadmium. Fragments corresponding to 26S rRNA were identified using suppression subtractive hybridization during screening for genes induced in tobacco plants upon a three-week exposure to 15 μM cadmium chloride. Extracts prepared from the above-ground tissues of cadmium-treated tobacco plants were supposed to contain exclusively polyadenylated mRNAs. Surprisingly, numerous polyadenylated fragments matching parts of 26S rRNA were identified and their presence was confirmed by Northern blot and cDNA amplification techniques. To our knowledge this is the first report on rRNA polyadenylation in plants
20

Effect of biotic and abiotic stress factors on the content of isoprenoids in the tobacco leaves

39%
Bajda A., Knopka-Postupolska D., Sirko A., Lewandowska M., Skorzynska-Polit E., Drazkiwicz M., Swiezewska E.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr Supplement 4
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