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A protocol has been developed for an efficient tissue culture cycle (callus induction, callus growth, plant regeneration and root induction) for date palm 'Khalas' using various plant growth regulators (PGR) in both liquid and solidified MS medium. The shoot explants showed high callus induction potential on MS medium supplemented with 45.24 ^M 2,4-dichlorophenoxyacetic acid (2,4-D) followed by 54.21 ^M 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). The highest frequency of shoot regeneration (85%) and number of shoots per an explant (5.6) were obtained on solid MS medium supplemented with 7.84 ^M N6-benzylaminopurine (BAP). Rooting (87.34%) was high on solid MS medium supplemented with 24.6 ^M indole-3-butyric acid (IBA). However, the root length was higher in MS liquid medium. This protocol will be useful for rapid, large-scale propagation of 'Khalas' date palm.
We report the use of laser-induced breakdown spectroscopy (LIBS) for determination of soil contamination due to effluents from the leather tanning industry in Kasur district of Punjab in Pakistan. The quantitative analysis was performed by making a calibration curve for Cr using LIBS spectral line at 427.48 nm. Chromium concentration of 839 mg·kg-1 in the vicinity of an effluent drain and 1,829 mg·kg-1 in the area of an old stagnant pool was found. The leaching of Cr due to seepage of industrial effluents from deteriorating bricklined drains in horizontal direction also was observed.
In the past 100 years, the annual global temperature has increased by almost 0.5ºC and is expected to increase further with time. This increase in temperature negatively affects the management of water resources globally as well as locally. Rain is an important phenomenon for agriculture, particularly in hilly areas where there is no feasible irrigation system. The present study is concerned with the analysis and modeling of the rain pattern, its variability, and prediction of monthly number of rainy days for the Abbottabad District, which is considered to be one of the greenest and most beautiful areas of Khyber Pakhtunkhwa, Pakistan, by incorporating both parametric and nonparametric techniques. Non-parametric statistical techniques are used for movement detection and significance testing; in this context, statistical tests were incorporated for inspection of homogeneity of rainy days among successive periods. A time series data for the period 1971-2013 was analyzed. Box Jenkins methodology and time series decomposition were applied for fitting the selected model, which was assessed for forecasting the monthly number of rainy days for 2015-2020. In this study several time series parametric and non-parametric approaches were applied to model rainfall data. The results showed that SARIMA (1, 0, 1) (0, 1, 1) was a better choice in predicting the monthly number of rainy days. Further analysis of the data suggests that January, March, May, July, and December have a considerable declining tendency in the number of rainy days.
In this study, the biocontrol abilities of water-soluble and volatile metabolites of three different isolates of Trichoderma (T. asperellum,T. harzianum and Trichoderma spp.) against soil borne plant pathogen Rhizoctonia solani were investigated both in vitro and in vivo. The results showed for the first time that mycelial growth inhibition of the pathogen was 74.4–67.8% with water-soluble metabolites as compared to 15.3–10.6% with volatile metabolites in vitro. In vivo antagonistic activity of Trichoderma isolates against R. solani was evaluated on bean plants under laboratory and greenhouse conditions. We observed that T. asperellum was more effective and consistent, lowering disease incidence up to 19.3% in laboratory and 30.5% in green house conditions. These results showed that three isolates of Trichoderma could be used as effective biocontrol agents against R. solani.
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