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1

Architecture of the neuronal nucleus

100%

Wilczynski G.

Acta Neurobiologiae Experimentalis

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2015

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tom 75

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nr Supl.

Studies in cultured cells have demonstrated the existence of higherorder epigenetic mechanisms, determining the relationship between expression of the gene and its position within the cell nucleus. It is unknown, whether such mechanisms operate in post-mitotic, highly differentiated cell types, such as neurons in vivo. Accordingly, we examined whether the intranuclear positions of Bdnf and Trkb genes, encoding the major neurotrophin and its receptor respectively, change as a result of neuronal activity, and what functional consequences such movements may have. In a rat model of status epilepticus we found that elevated neuronal expression of Bdnf after seizures is associated with its detachment from the nuclear lamina, and translocation toward the nucleus center. In contrast, the position of stably expressed Trkb remains unchanged after seizures. Our study demonstrates that activation-dependent architectural remodeling of the neuronal cell nucleus in vivo contributes to activity-dependent changes in gene expression in the brain.

2

Architectural changes in the neuronal nucleus during epileptogenesis

100%

Wilczynski G.

Acta Neurobiologiae Experimentalis

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2011

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tom 71

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nr S

Synaptic plasticity is the ability of neurons to change the strength of their synaptic connections according to the demands of the changing environment. The phenomenon underlies cognitive functions like learning and memory, and, in its aberrant form, plays important pathogenic role in brain disorders, especially in epilepsy. It is now firmly established that long-lasting synaptic plasticity involves dramatic changes in neuronal gene expression. The mechanisms of these changes are quite well understood at the level of cis- and trans-acting regulatory factors. In contrast, the potential role of higher-order nuclear architecture in genetic regulation of synaptic plasticity and epileptogenesis has not been explored. Therefore, we have examined the structure of the nuclei in the neurons of the rat hippocampus at different time points after acute seizures, using high-resolution morphological techniques and three-dimensional quantitative analysis. Our results indicate that there is prominent reorganization of the neuronal nucleus upon seizures, involving movements of highly expressed genes and chromosomal gene clusters. Such reorganization may lead to formation of molecular factories, in which transcription, splicing, and (possibly) quality control/export of pre-mRNA occur in concert.

3

High-resolutions light-microscopic studies of synaptic extracellular proteolysis

100%

Wilczynski G.

Acta Neurobiologiae Experimentalis

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2009

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tom 69

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nr 3

Proteolytic remodeling of perisynaptic environment, including extracellular matrix and adhesion molecules, is a novel important mechanism of synaptic plasticity. Among several enzymes participating in the phenomenon, the major role is played by matrix metalloproteinase 9 (MMP-9), a principal neuronal MMP. Recent evidence indicates MMP-9 being involved in long-term potentiation, learning and memory formation, as well as in pathological plasticity underlying epileptogenesis. Here, I present the results of the light-microscopic studies on MMP-9 localization at the synapses in situ, in both central and peripheral nervous systems. The data have been obtained using fl uorescent cytochemical techniques, including immunofl orescence, in situ hybridization and in situ zymography, with the aid of deconvolution-enhanced confocal microscopy. The results underscore the potential of light-microscope-based molecular morphology for studying synaptic function in situ.

4

Ultrastrukturalne i molekularne aspekty ekspresji jaderkowych genow rRNA

100%

Wilczynski G.

Postępy Biologii Komórki

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1995

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tom 22

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nr 1

73-100

5

Markham rotation of the electron microscopic images of cilia as an accessory method in differential diagnosis between Kartagener syndrome and Sturgess syndrome

63%

Sulikowska-Rowinska A., Wilczynski G.

Folia Histochemica et Cytobiologica. Supplement

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1996

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tom 34

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nr 1

6

Bialko rodziny 14-3-3, wystepowanie i wlasciwosci.

51%

Wilczynski G., Markiewicz E., Kulma A., Szopa J.

Postępy Biologii Komórki

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1998

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tom 25

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nr 1

9-32

7

Wpływ ostrej niewydolności oddechowej na skład lipidowy surfaktantu płucnego u psów

51%

Ledwozyw A., Jablonka S., Kadziolka W., Wilczynski G.

Medycyna Weterynaryjna

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1986

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tom 42

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nr 07

Исследовали липидный состав легочного сурфактанта, клеток, выстилающих легочные пузырьки и бронхиальное дерево, а также легочной ткани у собак с острой дыхательной недостаточностью, вызванной хирургичским удалением одного легкого. Исследования провели на 12 собаках-помесях, среднего веса тела ок. 20,5 кг. Материал фракционировали при помощи дифференциального сепарирования, отдельные классы липидов определяли методом тонкослойной хроматографии. Отметили, что у собак с острой дыхательной недостаточностью в сурфактанте и выстилающих клетках последовало понижение значительное концентрации фосфати- дилхолина (на ок. 25%), фосфатидилэтаноламина (на ок. 47%), почти полное исчезновение фосфа- тидилглицерола, а также рост количества фосфа- тидилсерина (на ок. 540%) и сфингомиелина (на ок. 200%). Не наблюдали таких изменений в фосфолипидах, экстрагированных из гомогената легочной ткани. Не обнаружили также значительных изменений во фракции неполярных липидов, ни в общем количестве неполярных липидов и фосфолипидов. Наблюдаемые изменения аналогичны изменениям, отмечаемым в случае острой дыхательной недостаточности у людей. Это внушает то, что примененная экспериментальная модель вызывания острой дыхательной недостаточности может быть пригодна в исследованиях патогенеза этого заболевания у людей.

8

Gelatinolytic activity is present in the interchromatin space of the neuronal cell nucleus

51%

Gawlak M., Gorkiewicz T., Wilczynski G.

Acta Neurobiologiae Experimentalis

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2009

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tom 69

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nr 3

Matrix metalloproteinases (MMPs) form an enzyme family which, by mainstream research, is implicated in extracellular matrix processing in physiological and pathophysiological conditions. Some of these proteins termed gelatinases, in particular MMP-2 and MMP-9, cleave gelatin as an artifi cial substrate. Surprisingly, a number of studies have revealed the presence of gelatinolytic activity in the cell nucleus. Although the phenomenon appears not to be artifactual, neither the identity nor the role of nuclear gelatinases has been established unequivocally. In the nervous system, nuclear gelatinolysis is detectable in normal conditions, yet it is induced by seizures, and stroke. We studied nuclear gelatinolytic activity by high resolution in situ zymography (ISZ) in sections of alcohol-fi xed, polyester wax-embedded normal rat brain. Ubiquitously distributed among the major brain areas the ISZ signal was present mainly in neurons. At high magnifi cation, our study revealed previously unrecognized mesh-like pattern of nuclear gelatinolytic which, by counterstaing with fl uorescent DNA-binding dye, represents an interchromatin space. The ISZ signal colocalized with the ribonucleoprotein compartment enriched in splicing components, identifi ed using an immunoreactivity of spliceosome assembly factor SC-35. This suggesting a function for MMPs in processes of gene-expression and/or RNA-processing and hypothetically involvement in remodeling of chromosome territories.

9

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Surgical treatment of large-cell neuroendocrine cancer of the lung in a patient with situs inversus totalis

51%

Prystupa T. K., Sagan D., Wilczynski G., Sawicki M.

Journal of Pre-Clinical and Clinical Research

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2018

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tom 12

|

nr 3

10

Potato plants over- and underexpressing 14-3-3 protein isoforms

51%

Wilczynski G., Kulma A., Szopa J.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 2

11

ARF protein regulates cAMP synthesis in potato

51%

Kulma A., Wilczynski G., Szopa J.

Cellular and Molecular Biology Letters

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1997

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tom 02

|

nr 2

12

Studying of 14-3-3 protein function in transgenic potato

51%

Wilczynski G., Kulma A., Szopa J.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 2

13

Characterisation of the role of the 14-3-3 protein family in potato plants

51%

Wilczynski G., Kulma A., Markiewicz E., Szopa J.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 2

14

Molecular cloning and sequencing og the cDNA encoding plant 22 kDa nuclease

51%

Szmidzinski R., Wilczynski G., Szopa J.

Acta Biochimica Polonica

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1994

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tom 41

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nr 2

15

Ultrastructural components of the nuclei of undifferentiated crypt base cells in the human small intestine - stereological of volumes

51%

Wilczynski G., Rowinski J., Sulikowska-Rowinska A.

Folia Histochemica et Cytobiologica. Supplement

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1996

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tom 34

|

nr 1

16

Cellular lokalization and activity of matrix metalloproteinase-9 in mouse model of in situ theromboembolic stroke

45%

Gawlak M., Szymanska M., Michaluk P., Kaczmarek L., Wilczynski G.

Acta Neurobiologiae Experimentalis

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2011

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tom 71

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nr S

Tissue plasminogen activator tPA is used for treatment of ischemic stroke patient. This thrombolytic agent resurrect blood supply to brain tissue, that is sensitive to oxygen and glucose deprivation. Administration of tPA is the only approved treatment and might be conducted up to 3 or 4.5 hours after ischemic stroke symptom onset. The benefits of it are time dependent and there is need for an improvement the timeliness of reperfusion. Some side effects of tPA is caused likely by its matrix metalloproteinase-9 (MMP-9) activation. Source and localization of MMP9 is still uncertain. This is investigated with high resolution in situ zymography. We use mouse model of in situ thromboembolic stroke and reperfusion as a clinically relevant. In this experimental set we identify oligodendrocytes and neurons but not astrocytes and microglia as a cellular source of gelatynolytic activity. Oligodendrocytes activity is most prominent. This is in opposition to literature. Activity of leukocytes is high however number of them suggest that their contribution to overall activity is negligible.

17

The 14-3-3 protein binds to the nuclear matrix endonuclease and has a possible function in the control of plant senescence

45%

Markiewicz E., Wilczynski G., Rzepecki R., Kulma A., Szopa J.

Cellular and Molecular Biology Letters

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1996

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tom 01

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nr 4

The nuclear matrices of plant cell nuclei display an intrinsic nuclease activity which is capable of nicking supercoiled DNA. Recently a cDNA encoding the 14-3-3 protein from Cucurbita pepo has been cloned and sequenced. The evidence that the recombinant 14-3-3 protein associates with DNase I and endogenous plant nuclease is presented. Evidence is also presented that the cloned 14-3-3 protein isoform, unique in its binding to nuclease within the 14-3-3 family, is located in the nuclei and in the nuclear matrix. Transgenic potato plants were created where the 14-3-3 protein derived from Cucurbita was overexpressed. An increase in tuber number and a decrease in tuber size in the transformants was also observed. The adenine nucleotide pool in leaves of transgenic plants was significantly reduced and they contain more chlorophyll and loose it slower when grown in the dark. A decrease in 14-3-3 protein content concomitant with an increase in nuclease activity in senescent plants was detected and this was markedly delayed in transgenic potato plants which overexpressed the 14-3-3 protein. It is proposed that a function of the isolated 14-3-3 isoform is the control of the nuclease activity and hence senescence.

18

Manipulating of 14-3-3 protein expression resultes in the changes of catecholamine content in potato plant

45%

Wilczynski G., Kulma A., Feiga I., Wenczel A., Szopa J.

Cellular and Molecular Biology Letters

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1998

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tom 03

|

nr 1

Recently transgenic potato plants were created where the 14-3-3 protein derived from Cucurbita pepo was overexpressed or potato endogenous 14-3-3 protein repressed. Detailed analysis of those plants suggested that a isolated 14-3-3 isoforms are involved in the control of plant senescence and carbohydrate metabolism. In this study carbohydrate content, adenine nucleotide level and catecholamine content in tubers and leaves of transgenic plants grown in greenhouse and in a field were compared. Overexpressing of 14-3-3 protein gave rise to increase catecholamine contents and soluble sugars in leaves and a reduction in tubers size and starch content. The repression of 14-3-3 synthesis led to opposite effect, a decrease in catecholamine, soluble sugars contents in leaves, and an increase in tubers size and starch content. It is proposed that 14-3-3 protein affects carbohydrate metabolism in potato via regulation of catecholamine synthesis.

19

Analysis of rabbit fed on wild type and genetic modified potato tubers

45%

Zuk M., Kulma A., Wilczynski G., Prescha A., Szopa J.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 2

20

The isolated 14-3-3 protein isoforms protect DNA against endonuclease[s] digestion in vitro

45%

Rzepecki R., Szalowska E., Klimek A., Wilczynski G., Szopa J.

Cellular and Molecular Biology Letters

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2000

|

tom 05

|

nr 2

Ograniczanie wyników

Architecture of the neuronal nucleus

Architectural changes in the neuronal nucleus during epileptogenesis

High-resolutions light-microscopic studies of synaptic extracellular proteolysis

Ultrastrukturalne i molekularne aspekty ekspresji jaderkowych genow rRNA

Markham rotation of the electron microscopic images of cilia as an accessory method in differential diagnosis between Kartagener syndrome and Sturgess syndrome

Bialko rodziny 14-3-3, wystepowanie i wlasciwosci.

Wpływ ostrej niewydolności oddechowej na skład lipidowy surfaktantu płucnego u psów

Gelatinolytic activity is present in the interchromatin space of the neuronal cell nucleus

Surgical treatment of large-cell neuroendocrine cancer of the lung in a patient with situs inversus totalis

Potato plants over- and underexpressing 14-3-3 protein isoforms

ARF protein regulates cAMP synthesis in potato

Studying of 14-3-3 protein function in transgenic potato

Characterisation of the role of the 14-3-3 protein family in potato plants

Molecular cloning and sequencing og the cDNA encoding plant 22 kDa nuclease

Ultrastructural components of the nuclei of undifferentiated crypt base cells in the human small intestine - stereological of volumes

Cellular lokalization and activity of matrix metalloproteinase-9 in mouse model of in situ theromboembolic stroke

The 14-3-3 protein binds to the nuclear matrix endonuclease and has a possible function in the control of plant senescence

Manipulating of 14-3-3 protein expression resultes in the changes of catecholamine content in potato plant

Analysis of rabbit fed on wild type and genetic modified potato tubers

The isolated 14-3-3 protein isoforms protect DNA against endonuclease[s] digestion in vitro