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Enteroccoci are Gram-positive, granular shaped bacteria classified as a separated genus called Enterococcus spp., which includes 20 species of microbes. They may exist as commensal organisms of the alimentary tract in chickens, turkeys, ducks, geese and pigeons. They have probiotic properties and are able to limit the colonization of the digestive tract by pathogenic bacteria. Enteroccoci strains have a variety of virulence factors: adhesion production, cytolysin, gelatinase, haluronidase, extracellular peroxides and extracellular surface proteins which may cause pathological lesions in poultry. E. faecalis, E. faecium, E. durans, E. hirae, E. cecorum species are pathogenic for poultry. Naturally occurring infections have been reported in chickens and ducks. Young birds are more susceptible. Enterococcal occurrence in chickens may induce low grade infections, septicemia and endocarditis, arthritis of thoracic vertebra and femoral necrosis in broilers (E. faecalis, E. durans, E. hirae, E. cecorum) septicemia and arthritis in ducks (E. faecalis, E. faecium).Sources of infection for poultry may be farm buildings, fowl-runs, or contaminated food. Alimentary and respiratory tracts or intramuscular injections are also infection routes and vertical means of infection are also possible. Pathological lesions in poultry caused by Enteroccoci cause losses due to an increased mortality rate, decrease in growth and difficulties in therapy as a result of the resistance of antibiotics to the microbes. Enterococcal infections in poultry are dangerous for humans because of the transmission of antibiotic resistance to some bacteria strains.
Avian adenoviruses (Aviadenovirus) belong to three groups. Group I contains five aviadenovirus species (A-E) with twelve serotypes isolated from fowls as well as duck adenovirus, pigeon adenovirus and turkey adenovirus. Group I of aviadenovirus plays no major role in mixed infections, although FadV-4 strains cause a dangerous disease in chickens - hydropericardium-hepatitis syndrome. Group II of the aviadenovirus includes the hemorrhagic enteritis virus of turkeys, marble spleen disease virus of pheasants and the splenomegaly adenovirus of chickens. In fact, the adenoviruses of this group are included in the Siadenovirus genus because the techniques of molecular hybridizations and DNA sequencing have indicated that there is a gene coding for sialidaze in their genome. The egg drop syndrome virus however, belongs to group III. Atadenovirus has been proposed as a name for this group of aviadenoviruses as it reflects the high adenine-thymidine (AT) content in their genome. The significance of adenovirus in pathology within poultry is constantly increasing.
The aim of the study was to evaluate 4-nitrophenylarsonic acid efficacy (Histostat® 50; Alpharma Trade Inc., USA) and metronidazol (Avimetronid; Polfa Grodzisk) in preventing turkeys histomoniasis. 10 turkeys with histomoniasis disorder and 10 healthy 10-week old turkeys' were divided into three groups (I, II, III). Turkeys from group I were the control group. The turkeys from groups II and III were administered Avimetronid at a dose of 2.5 g/kg and Histostat® 50 at a dose of 0.37 g/kg in their feed for 5 weeks, respectively. Clinical observations, anatomopathological examinations, parasitological examinations of cloacae swabs as well as hematological (RBC, WBC, leukogram) and biochemical examinations (ALT, AST, LDH) of the blood were carried out before the experiment and in the 5th week of its duration. The results indicated that 4-nitrophenylarsonic acid is highly effective in preventing histomoniasis and that metronidazol also has therapeutic proprieties. Although no side effects were noted in the turkeys receiving the examined 4-nitrophenylarsonic acid, it nevertheless seems that there is a danger of arsenic compounds cumulating in turkeys' organisms.
The aim of the study was to determine the influence of administering compound feed containing various fructo-oligosaccharides (FOS) on the health of turkeys infected with Hemorrhagic enteritis virus (HEV) and with Salmonella Typhimurium (ST). Investigations were performed on 270 young male BUT-9 turkeys which were divided into three groups (I-III) consisting of 90 birds in each group. Group I received a compound feed without FOS, while group II and III received feed with a 2% addition of chicory fine flour and standard fructo-oligosaccharide from chicory, respectively. At the age of eight weeks 13 birds from every group were injected per os with HE virus at a dose of 104,6EID50/ml. After five days three birds from each group were slaughtered with the aim of defining their susceptibility to HEV and the 10 remaining turkeys were infected with ST: 1 ml of a bacterial suspension PBS with a concentration of 9×109 jtk/ml into the croup. The birds were clinically observed for 12 days. Blood samples were controlled from five birds from each group on days 5 and 12 following infection, after which three of the turkeys were slaughtered, anatomopathologically examined and cuttings of the liver and caecum were collected for bacteriological examination. The results of the study indicate that the turkeys which received feed containing FOS did not succumb to ST infection because, five days following infection, bacteria was isolated only from the caecum, whereas it was additionally isolated from the liver in the case of turkeys who had received feed not containing FOS. The biochemical results from the groups of turkeys fitting within physiological norms and receiving feed containing FOS proves that there was no general ST infection and that FOS do not negatively influence turkeys' organisms. Five days after infection there was an increase of LDH activity in the three groups of turkeys receiving feed without FOS. The results of the study do not unanimously prove that FOS slow down ST infections but they do encourage further research on the issue.
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