Wyniki wyszukiwania

1

Enerthecoma disimilisna new rhynchodid ciliate from freshwater gastropod viviparus dissimilis

100%

Mahmood M.

Acta Protozoologica

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1980

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tom 19

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nr 3

2

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Effects of micronutrients (Cu, Zn, Mn, and Fe) on the growth of Spathoglottis plicata plantlets

51%

Yasin Z. A. M., Mahmood M., Shaharudiin N. A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2017

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tom 98

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nr 1

3

An efficient protocol for particle bombardment-mediated transformation of Centella asiatica callus

51%

Lai K.-S., Abdullah P., Yusoff K., Mahmood M.

Acta Physiologiae Plantarum

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2011

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tom 33

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nr 6

In this study, we report an optimization of particle bombardment transformation system for Centella asiatica callus. A total of eight parameters affecting the genetic transformation system were optimized using the synthetic green fluorescent protein (sGFP) as a reporter driven by the CaMV 35S promoter. The results indicated that DNA delivery conditions of 9-cm target distance, 1,100 psi helium pressure, 1.0 µm gold particles size, 27 mmHg chamber vacuum pressure, 2 times number of bombardment, spermidine as precipitation agent, 60 h postbombardment incubation time and 2 µg plasmid DNA concentration were optimal for C. asiatica callus transformation. The expression of sGFP was monitored using fluorescence microscope and further confirmed using RT-PCR. This optimized genetic transformation system is applicable for rapid transient gene analysis and transgenic C. asiatica production.

4

Azjatyckie rosliny lecznicze i kosmetyczne. Cz.I. Wakrota azjatycka Centella asiatica

51%

Guzewska J., Furmanowa M., Mahmood M.

Wiadomości Zielarskie

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2000

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tom 42

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nr 06

9-10

5

Exogenous proline significantly affects the plant growth and nitrogen assimilation enzymes activities in rice (Oryza sativa) under salt stress

51%

Teh C.-Y., Shaharuddin N. A., Ho C. L., Mahmood M.

Acta Physiologiae Plantarum

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2016

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tom 38

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nr 06

6

Heterologous expression of hemagglutinin-neuraminidase protein from Newcastle disease virus strain AF2240 in Centella asiatica

51%

Lai K. S., Yusoff K., Mahmood M.

Acta Biologica Cracoviensia. Series Botanica

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2012

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tom 54

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nr 1

We explored the use of the medicinally important plant Centella asiatica for expression of hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) strain AF2240. HN protein is the principal target for subunit vaccine development against NDV. The full-length HN gene was cloned into a plant expression construct driven by the CaMV 35S promoter and C-terminal fusion of green fluorescence protein (GFP) as reporter system. The recombinant expression construct was transformed via particle bombardment into C. asiatica callus. Transformants were screened using GFP and selected on MS medium supplemented with 15 mg/l hygromycin. The ~1.8 kb HN mRNA transcript was detected on the putative transformants using RT-PCR. The presence of HN protein expression was further confirmed through dot blot analysis using anti-NDV chicken serum. Here we report, for the first time, the use of a novel medicinal plant as a new platform for HN protein expression.

7

Basidiospore and protoplast regeneration from raised fruiting bodies of pathogenic Ganoderma boninense

51%

Govender N. T., Mahmood M., Seman I. A., Mui-Jun W.

Polish Journal of Microbiology

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2016

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tom 65

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nr 3

Ganoderma boninense, a phytopathogenic white rot fungus had sought minimal genetic characterizations despite huge biotechnological potentials. Thus, efficient collection of fruiting body, basidiospore and protoplast of G. boninense is described. Matured basidiocarp raised under the glasshouse conditions yielded a total of 8.3 × 10⁴ basidiospores/ml using the low speed centrifugation technique. Mycelium aged 3-day-old treated under an incubation period of 3 h in lysing enzyme from Trichoderma harzianum (10 mg/ml) suspended in osmotic stabilizer (0.6 M potassium chloride and 20 mM dipotassium phosphate buffer) yielded the highest number of viable protoplasts (8.9 × 10⁶ single colonies) among all possible combinations tested (regeneration media, age of mycelium, osmotic stabilizer, digestive enzyme and incubation period).

8

Identification and expression profiling of a novel Kunitz trypsin inhibitor (KTI) gene from turmeric, Curcuma longa, by real-time quantitative PCR (RT-qPCR)

39%

Chan S.-N., Abu Bakar N., Mahmood M., Ho C.-L., Dzaki N. M., Shaharuddin N. A.

Acta Physiologiae Plantarum

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2017

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tom 39

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nr 01

Ograniczanie wyników

Enerthecoma disimilisna new rhynchodid ciliate from freshwater gastropod viviparus dissimilis

Effects of micronutrients (Cu, Zn, Mn, and Fe) on the growth of Spathoglottis plicata plantlets

An efficient protocol for particle bombardment-mediated transformation of Centella asiatica callus

Azjatyckie rosliny lecznicze i kosmetyczne. Cz.I. Wakrota azjatycka Centella asiatica

Exogenous proline significantly affects the plant growth and nitrogen assimilation enzymes activities in rice (Oryza sativa) under salt stress

Heterologous expression of hemagglutinin-neuraminidase protein from Newcastle disease virus strain AF2240 in Centella asiatica

Basidiospore and protoplast regeneration from raised fruiting bodies of pathogenic Ganoderma boninense

Identification and expression profiling of a novel Kunitz trypsin inhibitor (KTI) gene from turmeric, Curcuma longa, by real-time quantitative PCR (RT-qPCR)