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In vivo and in vitro effects of ethephon on some oxidative enzymes in spinach leaves

100%

Ozturk L., Kufrevioglu O. I., Demir Y.

Acta Physiologiae Plantarum

2008

tom 30

nr 1

In vivo and in vitro effects of ethephon, an ethylene-generating compound, on some oxidative enzymes; polyphenol oxidase (PPO, 1.10.3.1), peroxidase (POD, 1.11.1.7) and catalase (CAT, 1.11.1.6) in spinach leaves were investigated. The plants were grown under controlled conditions in sand culture in 60 days. Ethephon was applied as two doses at 0.1 and 1 mM. The leaves were harvested in the first and fifth days after ethephon treatment. In order to determine the effect of ethephon on the enzymes in vitro, the enzymes from leaves in control group were partially purified. In vivo, PPO activities were increased significantly at both concentrations whereas significant enhancement of POD activity was determined only at 1 mM. CAT activity was significantly reduced by ethephon through time. In vitro, POD and CAT activities increased slightly at 0.033 and 0.33 mM ethephon concentrations, as PPO activity did not increase at the same concentrations. But these activities of enzymes decreased significantly at 33.3 mM of ethephon concentration for POD and PPO and 26.6 mMfor CAT. As a result, the increase of activity for PPO and POD or decrease of activity for CAT in vivo may be caused by not direct inhibition or activation of enzymes but a mechanism at transcriptional level induced by ethylene.

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Fatty acids and physiological responses of corn leaves exposed to heavy metals

76%

Kisa D., Ozturk L., Saglam N., Kayir O., Elmastas M., Doker S.

Acta Scientiarum Polonorum. Hortorum Cultus

2020

tom 19

nr 3

Heavy metals affect biochemical pathway by changing the fatty acid composition in plant cells. The high concentration of heavy metals impresses biochemical pathway and changes fatty acid compositions of plant cells. Fatty acids participate in various biological processes and have the functional role in regulating membrane functions in plants. In the present study, heavy metal content was analyzed with ICP-MS, fatty acid composition was investigated with GC and physiological parameters were determined with spectrophotometrically in the leaves of tomato subjected to increasing doses of heavy metals. In this study, the treatment of heavy metals on the growth medium changed the fatty acid contents of corn. The application of Cu significantly increased the level of palmitic acid and oleic acid. The treatment of Pb raised the content of oleic acid, whereas it significantly decreased the content of α-linolenic acid and erucic acid at 20 and 50 mg kg⁻¹, respectively. The addition of Cd significantly increased the level of oleic acid and linoleic acid; however, it significantly decreased the content of α-linolenic acid and erucic acid. Cu and Pb significantly raised the proline content. The application of Cu and Cd showed similar effect on hydrogen peroxide and the higher doses of them increased the content of H₂O₂. The level of lipid peroxidation significantly increased in response to all applied concentration of Cu. The results obtained in this study show that the aapplication of heavy metals changed the content of fatty acids, particularly that of oleic acid significantly increased in response to them. The levels of proline and lipid peroxidation generally increased together with oleic acid and palmitic acid in the leaves in reply to copper.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Acta Scientiarum Polonorum. Hortorum Cultus

2 Ozturk L.

1 Demir Y.

1 Doker S.

1 Elmastas M.

1 Kayir O.

1 Kisa D.

1 Kufrevioglu O. I.

1 Saglam N.

1 2020

1 2008

Wyniki wyszukiwania

In vivo and in vitro effects of ethephon on some oxidative enzymes in spinach leaves

Fatty acids and physiological responses of corn leaves exposed to heavy metals