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The aim of this study was to determine the effect of the calpastatin (CAST) and ryanodine receptor (RYR1) genes polymorphism on carcass and meat quality traits in Pietrain crossbred pigs. No significant differences in the traits examined were identified between pigs with the genotypes CT and CC at the locus RYR1. A significant association occurred between the polymorphisms CAST/PvuII and CAST/RsaI, and the traits characterizing the quality of carcass and composition of meat. Meat from pigs with the genotype AB CAST/PvuII had a significantly higher pH determined 24 and 48 h post mortem, lower drip loss, lower yellowness (b*) and a lower protein content compared to meat from pigs with the genotype AA. In addition, the meat from pigs with the genotype EF CAST/RsaI had a significantly higher pH 48 h post mortem, lower drip loss and lower yellowness (b*) than that of pigs with the genotype EE. The results indicate that several quality and composition traits of fresk meat from the offspring by Pietrain boars are significantly related to the CAST genotype.
The experiment was carried out on 95 samples of the longissimus dorsi muscle (LD). They were collected from carcasses classified to meatiness classes E, U and R (24, 52 and 19 samples, respectively) of pure-bred pbz porkers, hybrids of white pigs sows (wbp x pbz) with Pietrain boars and those of Dutch pigs (Landrace x wb) x wb. After slaughter, blood samples were collected in porkers for DNA analysis to identify animals carrying a stress-susceptibility gene (RYR1). After carcass cooling, meat quality detailed evaluation (dissection) was carried out according to methods given by Walstra and Merkus [1996], on the grounds of which carcasses were classified into respective EUROP system classes. Meat quality was determined as well, pH1, pHk and electric conduction; meat colour, wateriness, hardness and consistency were assessed. Moreover, a measurement of the volume of free drip was made, meat wateriness was determined, as well as drip index and colour parameters were calculated. Also, meat basic chemical composition and meat water-soluble protein content were analysed. Furthermore, the frequency of meat with PSE and DFD defects was also determined. Carcasses of porkers from class E and U more frequently showed an incidence of meat defects of PSE, partial PSE and partial DFD types. Higher frequency of the incidence of normal meat was found together with an increase in carcass meatiness. All carcasses of animals with recessive homozygote genotype (nn) had the highest meatiness (classes E and U). The genetic vulnerability of the porkers to produce PSE meat was found in 25% of the studied material. In the group of porkers with the highest meatiness, class E, only 17% (out of 17 animals with genetic burdens 11 nn and 6 Nn), or 3 individuals were found after slaughter to have PSE meat.
The studies were carried out on 98 carcasses obtained from pure-bred Polish Landrace (PL) hogs, from crosses of (PL x Polish Large White) sows with Pietrain boars, as well as from crossbreds of Dutch breeds (Landrace x Large White) x Large White. The pigs were slaughtered as soon as they reached 103 kg of body weight, and during the slaughter blood samples were collected for DNA analysis in order to identify individuals carrying the stress-sensitivity gene (RYR1). The carcasses were dissected immediately after cooling. Samples of LD muscle from the right carcass, at the area of the 1st to 4th lumbar vertebra, were collected for pork quality evaluation, i.e. sensory analysis of raw meat, as well as pH1, pHk, and electric conductivity measurements. Moreover, drip loss, water-binding and meat plasticity were determined, and drip index as well as colour parameters were obtained.
Meat samples, numbering 72 in total, differentiated in respect of quality and collected from longissimus lumborum muscle of 72 carcasses of porkers (36 gilts and 36 hogs) approximating in their exterior Polish Large White and Polish Landrace crossbreeds originated from a large-scale farm at Kolbacz and slaughtered on an industrial processing line, were examined. Approximately 45 min after slaughter, pH1 value was determined between the 4th and the 5th lumbar vertebra. Meat samples were collected after approximately 24-h cooling and stored for ca. 24 h at 0º to 4º C. Approximately 48 h from the slaughter, sensory examination of colour, wateriness and springiness of raw meat was performed, followed by determination of water-binding capacity, pHu and dry matter, total protein and fat percentages, as well as that of water-soluble protein, after double mincing the meat. Using Mini Scan XE Plus 45/0 apparatus, meat colour parameters were determined in CIE L*a*b* and CIE L*C*h scales applying two illuminant/observer combinations used most frequently in meat colour measurements, i.e. illuminant C and standard observer 2º as well as illuminant D65 and standard observer 10º. Colour measurements were made after 20-min storage of the samples at 0º do 4º C. Colour parameters closely connected with meat quality proved to be lightness (L*) and yellowness (b*), and slightly less chroma (C*), whereas a* (redness) and h°(hue) parameters were linked with meat quality to the least extent, which in general showed medium and low correlation with meat quality traits. The application of illuminant D65 and observer 10º for measurements of meat colour proved to be more suitable in the case of parameter a* and C*, whereas the use of illuminant C and observer 2º in the case of parameter hº.
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