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Mycoplasma mastitis is one of the most pressing problems in herds of cows, especially in regions of increased milk production. Clinical signs in infected animals are not specific and the diagnosis of its causes should be based on laboratory testing. The classical scheme of investigation used in laboratories does not allow for their detection and should be extended to one of their methods of detection. Increased opportunities to detect mycoplasma are attained by proper storage of the milk samples.
In Poland, the quality and hygiene of honey, as a foodstuff of animal origin, is subject to the supervision of three state authorities: Veterinary Inspection, National Sanitary Inspection and Agricultural and Food Quality Inspection. The role of Veterinary Inspection consists in supervising the conditions of acquisition, storage and marketing of honey, as well as in constant monitoring aimed at the detection of illicit substance content. Owing to the peculiar character of apiculture, it is not always possible to completely adjust the process of honey production to the requirements which apply to the production of other foodstuffs under the common legal regulations. Therefore, proper supervision involves not only the enforcement of rules and orders, but also the assistance provided to honey producers in ensuring the safety of honey production and the hygiene of the product in order to protect the consumers.
The development of new and modification of previously existing food quality systems are the result of growing consumer demand for quality food. The EU systems guaranteed traditional specialty and geographical indications have been subjected to verification and are designed for these new legal requirements, which are discussed in the article. Moreover, in this article five national systems approved by the Minister of Agriculture and Rural Development are presented and characterized. Differences between the UE and national food quality systems have been indicated.
Turkeys carcasses at selected point after slaughter on dressing line in poultry were sampled and analyzed for Salmonella. These slaughter turkeys came from the northeast part of Poland. The examinations were carried out in each month of 2009. Three hundred turkeys were selected at random from a commercial slaughter line, immediately after completing the cooling process. The percentage of these 300 turkeys from which Salmonella spp. were isolated was relatively high (8.3%; Salmonella positive results were observed in 25 cases). The lowest Salmonella spp. rate (1.3 %) for slaughter birds was found in the fourth quarter, and the highest contamination rate at 18.6% was found, in the third quarter. The serological types of Salmonella spp. isolated from the whole turkey carcasses were S. Saintpaul, S. Senftenberg, S. Anatum, S. Heidelberg, S. Hadar, S. Typhimurium and S. Infantis.
Examination of raw milk samples provides important information to breeders, free practice veterinarians, inspectors, and processing facilities. Milk samples are collected at different stages of the dairy chain. The paper discusses most of the directions in which raw milk samples are tested at the stages of milk collection and storage. In order to standardize the proceedings prior to laboratory analysis, the paper cites regulations and guidelines specifying the appropriate sampling of milk, as well as the transport and storage of samples. Each sample collected for testing should be accompanied by a document that identifies the sample and the batch from which it was collected. Proper pre-analytical procedures are an important factor influencing the results of laboratory tests.
Hypodermosis is responsible for significant economic losses and remains a current problem in animal husbandry. This study determined the extent of Hypoderma spp. invasion in north-eastern Poland in dairy herds. The extent of the invasion, determined on the basis of positive results of an ELISA assay (Bovine Hypodermosis Milk Screening of Institut Pourquier), varied in herds. Statistical analysis performed on the results of milk productivity and content of basic components revealed a decrease in the milk yield and changes in milk protein in animals revealing positive, doubtful and negative results. Statistically significant differences were absent as regards the content of milk fat or milk total solids. The milk yield and the content of particular milk components were determined using the AT4 method, as part of a control program carried out by the Polish Federation of Cattle Breeders and Dairy Farmers (PFHBiPM).
The aim of this study was to determine the kinetics of the survival of the test strain of Staphylococcus aureus in the product investigated. Yogurt samples were contaminated with S. aureus to an initial level of 103-104 cfu/g. The samples were then stored at four temperatures: 4, 6, 20, 22oC. During storage, the number of S. aureus forming colonies in a gram of yogurt was determined every two hours. Based on the results of the analysis culture the curves of survival were plotted. Three primary models were selected to describe the kinetics of changes in the count of bacteria: Cole's model, a modified model of Gompertz and the model of Baranyi and Roberts. Analysis of the model fit carried out based on the average values of Pearson's correlation coefficient, between the modeled and measured values, showed that the Cole's model had the worst fit. The modified Gompertz model showed the count of S. aureus as a negative value. These drawbacks were not observed in the model of Baranyi and Roberts. For this reason, this model best reflects the kinetics of changes in the number of staphylococci in yogurt.
Invasions of gastrointestinal nematodes in dairy cows may affect animals productivity. The most frequently detected internal parasite of dairy cattle is Ostertagia ostertagi. The objective of this study was to determine O. ostertagi invasion extensiveness in selected herds of dairy cattle, with special consideration to cows being in the first lactation, and to analyze the milk yield and contents of basic constituents of milk originating from sero-positive cows. Five herds of dairy cattle (403), with different populations of cows, were selected for the study. Invasion extensiveness in particular herds was determined and ranged from 11.9% to 27.27%. Cows being in the first lactation, the udder milk of which was shown to contain anti-O. ostertagi antibodies, were producing on average 470 kg of milk annually less than cows being in the same lactation period. The analysis of results did not confirm the statistical significance of this difference, likewise it did not demonstrate any statistically significant differences in contents of fat, protein and dry matter. Despite a lack of the statistical significance a producer suffers great economic losses. The conducted study proves that the occurrence of O. ostertagi invasion in herds of dairy cattle is a global problem and that it affects cost-effectiveness of milk production.
The aim of the study was to determine the kinetics of the growth and survival of Staphylococcus aureus in regional rennet ripened cheese manufactured from unpasteurized goat’s milk. Cheese samples were contaminated with S. aureus and stored at 4, 6, 20, or 22°C. During storage, pH balance, water activity, and the number of S. aureus cells per gram of the sample were determined. On the basis of the data, models of growth and survival of staphylococci in cheese were described, using a system of three coupled differential equations. The analysis of the measurements and fitting of the model demonstrated that the model consisting of a system of three differential equations was a good fit for the experimental data on water activity, pH, and the number of bacteria.
The aim of the study was to measure the frequency of occurrence of Salmonella spp. in raw milk, to identify their serotype, as well as to determine their antibiotic resistance and the presence of Salmonella plasmid virulence (spv) genes. Out of 300 bulk tank milk samples, 5.3% were contaminated with Salmonella spp. All strains isolated belonged to the serovar S enteritidis, as confirmed by serotyping and molecular methods. The presence of spv genes was determined by PCR. Spv genes were present, in different patterns, in all strains tested. SpvA gene was present in all isolates (100%), spvB in 56.25%, spvC in 62.5%, spvD in 75%, and spvR in 56.25%. Antibiotic resistance was evaluated according to the NCCLS recommendations. All isolates were sensitive to ciprofloxacin (CIP), gentamicin (GE), and chloramphenicol (CH). Thirteen strains were resistant to ampicillin (AMP), 8 to erythromycin (E), 1 to doxycycline (DO), and 1 to tetracycline (TE). Different frequency of occurrence of the spv genes in Salmonella strains isolated from raw milk demonstrates their high adaptability. As many as 87.5% of isolates showed resistance to at least one of the antibiotics tested.
Paratuberculosis or Johne’s disease is a chronic granulomatous enteric disease afflicting cattle, sheep, goats and other ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). Calves are infected mainly via an oral route, especially from manure and environment contamination by infected adult cattle. It is generally assumed that calves rarely shed MAP bacteria and that calf-to-calf transmission is of minor importance. The aim of the study was to evaluate occurrence of faecal excretion of MAP by calves born to MAP-positive and MAP-negative dams in a low MAP prevalence herd. The study involved 66 dam-calf pairs. Samples of faeces were collected: for each cow once about 2-3 weeks to parturition, and for each calf 3 times at 2-5, 180-185 and 360-365 days of age. All samples were studied for the presence of MAP by culture or by PCR of IS900 insertion sequence MAP DNA. Faecal samples of the dams demonstrated 15/66 dams with at least one positive result. Analysis of MAP shedding prevalence in the faeces of calves originating from MAP-positive and MAP-negative dams shows that in 15 MAP-positive dams: 10 out of 15 calves (66.66%) at the age of 180-185 days and at the aged of 360-365 days 11 out of 15 calves (73.33%) were MAP-positive in faecal samples. In the case of MAP-negative dams: 7 out of 51 calves (13.72%) at the age of 180-185 days, and 8 out of 51 calves (15.68%) at the age of 360-365 days were MAP-positive. MAP-infection cases in calves coming from infected and healthy dams prove the occurrence of calf-to-calf transmission and that contact with infected calves increases the risk of MAP spreading among healthy calves.
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