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Plant functional traits are linked with environmental factors, individuals and ecosystem structure and functions as plants respond and adapt to the environment. Here, the whole-plant traits (plant height and plant biomass), leaf morphological (leaf area, leaf dry mass and specific leaf area) and chemical traits (leaf carbon, nitrogen, phosphorus contents, C/N and N/P ratio) of six dominant species (Cynanchum komarovii Al, Euphorbia esula Linn, Glycyrrhiza uralensis Fisch, Lespedeza potaninii Vass, Agropyron mongolicum Keng and Stipa breviflora Griseb) were studied in the desert grassland of China, with a grazing experiment including four grazing intensity (0.00, 0.45, 1.00, 1.50 sheep ha–1). The effect of grazing on leaf morphological traits were significant for the six dominant species, while the effects on whole-plant traits were highly significant for S. breviflora, A. mongolicum, G. uralensis, L. potaninii and C. komarovii. Three of the six species (S. breviflora, A. mongolicum and L. potaninii) decreased in plant height (PH) with increasing grazing intensity, while specific leaf area (SLA) and leaf N (Nmass) showed the opposite trend. The whole-plant traits were significantly positively correlated with leaf dry mass (LM), but significantly negatively correlated with leaf nitrogen and phosphorus contents. The first principal component analysis (PCA) axis corresponding to plant size (PH and PB) and leaf size (LA and LM), while the second axis of PCA to leaf chemical traits (Nmass, P, C and N/P). Variations of plant traits in response to grazing were mainly explained by size trait and chemical traits. The functional traits cannot be the only basis for predicting plant species in response to grazing, and a functional analysis of the trade-off between plant traits is also needed.
The dried roots of Radix Astragali are widely used in traditional Chinese medicine, and flavonoids present in the root of this herb have been implicated in its bioactivity. We subjected 2-year-old Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao to a progressive drought stress over 14 days of water withholding and studied the physiological and biochemical responses and secondary metabolite accumulation. Results indicated that drought stress reduced relative water content, reduced yield, but increased electrolyte leakage, malondialdehyde, proline and soluble sugar content. Mild and moderate drought stress enhanced some antioxidative enzyme activity to protect plants from damaging, but antioxidative enzyme activity was limited by severe stress. The calycosin- 7-O-β-D-glycoside and ononin content of plant roots was enhanced with degree of drought stress, whereas calycosin and formononetin levels did not differ significantly until 14 days. These results demonstrate that Radix Astragali can adapt to water stress by up-regulating antioxidant enzymes and accumulation of osmotic agents, and a certain degree of drought stress can enhance accumulation of some flavonoids, potentially facilitating higher yields of pharmacological activity of calycosin-7-O-β-D-glycoside production.
Salicylic acid (SA), a key signaling molecule in higher plants, has been found to play a role in the response to a diverse range of phytopathogens and is essential for the establishment of both local and systemic-acquired resistance. Recent studies have indicated that SA also plays an important role in abiotic stress-induced signaling, and studies on SA-modulated abiotic tolerance have mainly focused on the antioxidant capacity of plants by altering the activity of anti-oxidative enzymes. However, little information is available about the molecular mechanisms of SA-induced abiotic stress tolerance. Here, we review recent progress toward characterizing the SA-regulated genes and proteins, the SA signaling pathway, the connections and differences between SA-induced tolerances to biotic and abiotic stresses, and the interaction of SA with other plant hormones under conditions of abiotic stress. The future prospects related to molecular tolerance of SA in response to abiotic stresses are also further summarized.
This paper aims to introduce the new constraints of PM2.5 into the environmental efficiency research framework, based on the provincial panel data of China during 2001-12. The authors examine environmental efficiency with and without consideration of PM2.5, conduct hypothesis tests on these environmental efficiencies, and analyze the energy savings and haze reduction. The results indicate that the overall environmental efficiency score is inefficient. The average environmental efficiency scores are 0.706 and 0.867 with and without the consideration of PM2.5, respectively, and omitting PM2.5 would result in biased environmental efficiency change. In addition, empirical analysis shows that there is an unbalanced regional development and significant differences among provinces in environmental efficiency. Last but not the least, the potential savings of energy, water, and regional haze reduction average 24.26%, 39.16%, and 26.49%, respectively.
The chemical fraction of elements has been widely employed in the study of soil chemistry, plant nutrition, and environmental science. For this study we conducted a comprehensive survey on trace elements (Hg, As, Cd, Cr, Pb, Cu, Zn, Mn, Se, Mo, Co, etc.) from the rock tea gardens in Wuyishan, southeastern China. Our results demonstrate that: 1) The contents of heavy metals such as Hg, Pb, As, and Cd in the soil meet the environmental requirements for the growing area of tea (NY/T 853-2004), and the soil in the studied area contains sufficient Mn, Mo, and Se for tea plant growth, with a slight lack of Co. 2) The exchangeable fraction is determined to be the dominant fraction of Cd, which originates from an anthropogenic source and possesses high bioavailability from elemental speciation analysis. 3) The heavy metal contents in all the tea leaf samples are within the safe range, and the average values of the element enrichment coefficients of the tea leaves decrease in the order: Mn > Cu > Hg > Zn > Se > Cd > Mo > Co > As > Pb > Cr.
The effect of ultraviolet-B (UV-B) radiation on Antarctic phytoplankton has become an attractive ecological issue as a result of annual springtime ozone depletion. The effects of UV-B radiation on the growth and antioxidant enzymes were investigated using Antarctic sea ice microalgae Chlamydomonas sp. ICE-L as the material in this study. The results demonstrated that UV-B radiation could notably inhibit the growth, especially at high UV-B radiation intensity (70 µW cm⁻²). Malondialdehyde and O₂˙⁻ content in ICE-L increased rapidly in early days (1–3 days) exposed to UV-B radiation enhancement, then decreased rapidly. In the stress of UV-B radiation enhancement, the superoxide dismutase, peroxidase and Catalase activities of 1–4 days in ICE-L were obviously higher than those in the control, and their activities became higher at high UV-B radiation intensity (70 µW cm⁻²). These enzymes activity of 7 days would kept stable at low UV-B radiation intensity (35 µW cm⁻²), but kept high level at high UV-B radiation intensity (70 µW cm⁻²). However, the ascorbate peroxidase activity in ICE-L kept stable under the stress of UV-B radiation enhancement. The above experimental results indicated that the antioxidant enzyme system played an important role in the adaptation of Antarctic ice microalgae under the UV-B radiation change of Antarctic ecosystems.
To obtain sustainable aquaculture, developing appropriate treatment processes for wastewater is essential. In this study, two three-stage hybrid wetland systems were configured to treat aquaculture effluent. The two systems added with or without artificial aeration were operated under a high HLR (8.0 m/day) with a short retention time (0.96 h). By the results, COD could be effectively removed by both the systems, and it had been significantly enhanced by continuous aeration (air:water ratio being 7.5:1). For nitrogen (N) compounds, NH4+-N concentration of outflow compared to the inflow was elevated in most cases under the nonaerated condition, but an opposite trend was observed for the aerated state, which indicated that dissolved oxygen (DO) required for nitrification in the natural bed was more insufficient at the high organic loading rate. TN mass removal was efficient without aeration, but it significantly declined after enhancement, possibly due to the resuspension of trapped organic N promoted by the strong airflow. The artificial aeration also significantly improved the treatment performance on phosphorus. By canonical correspondence analysis (CCA), the first-order removal rate constants of various pollutants were significantly correlated to the measured environment of the inflow. Under the high constant HLR accompanied by low DO, pollutant loading rate became the first dependent factor on removal rate for such a rapid filtration system.
An early light-inducible protein (GbELIP) isolated from immature ginkgo leaves by rapid amplification of cDNA ends method contains a 570 bp open reading frame encoding a 189 amino acid protein, with significant homology to early light-inducible proteins from other plant species. The genomic DNA of GbELIP gene contains three exons and two introns. Southern blotting revealed that GbELIP is a singlecopy gene in ginkgo. Light, defense and stress responsive element were found in the promoter region of GbELIP. GbELIP expressional patterns were detected by Real-time PCR at various conditions. ELIP expressions were variable at different time points of 1 day and increasing significantly at the commencement of light illumination then dropping to a basal level gradually. ELIP expression in small size leaves (1–1.5 cm width and 7-day age) was higher then middle (2–3 cm width and 15-day age) and large (4 cm width and 30-day age) size leaves. It revealed that ELIP expression was under the control of developmental regulation. Under temperature and light intensity treatments, GbELIP transcripts accumulation may relate to photoinhibition. The peak expression of GbELIP appeared later by chilling than heating Meanwhile, GbELIP expression under high light was higher than low light at both two temperatures. GbELIP expression was also increased by wounding and methyl jasmonate (Me-JA) treatment, but not corresponded with increasing malondialdehyde (MDA) concentration. In conclusion, GbELIP appears to be induced by an imbalance between chlorophyll formation and degradation during development or under abiotic stress. These results suggest that GbELIP may function in response to environmental signals, possibly regulating responses to abiotic stresses.
As a type of natural energy resource, wind power is used in the modern implementation of wind-assisted technologies as a method for reducing the fuel consumption and environmental pollution of ocean-going ships. In order to promote the full usage of ocean wind energy for cargo ships, an innovative type of ship propulsion-assisted wing sail is proposed in this paper. The propulsion efficiency of this new wing sail can be increased by enlarging its area in both the transverse and vertical directions in good weather conditions, and it can be folded up automatically in poor weather conditions, improving the sailing safety of the ship. The sail parameters relating to the gaps and rotation angles between different parts of the wing sail are compared, and the values giving the best aerodynamic performance are identified using CFD simulation technology. The results for the lift and drag coefficients for the new wing sail at different attack angles are also compared with those of traditional aerofoil sails, including an arc-shaped rigid sail and a variable-camber sail proposed in 2015. From the viewpoint of the sailing performance of the vessel, our results demonstrate that this new type of wing sail has good aerodynamic performance and can reduce fuel costs for commercial vessels
Grinding materials as a pretreatment contribute to successful composting. Here the grinding ratio of 4 raw materials and compost samples with different maturity degrees were compared and discussed. Results revealed that the highest grinding ratio was obtained when drying the 3 straws into constant weight, but keeping water content around 10% for sewage sludge. The carbon-to-nitrogen ratio (C/N) was an important factor impacting the grinding ratio of raw materials except water content. Different straw materials had a decreased grinding ratio from the highest to the lowest in the following order: corn stover > rice straw > wheat straw. The grinding ratio of fermented compost significantly decreased lower than about 50% when the water content was higher than 30%, whereas no significant difference was observed when the water content was lower than 20%. Compared with original materials, mature compost had a better grinding ratio. Further regression analysis implies that grinding ratio had a negative linear orrelation with total organic carbon (TOC), C/N, hemicellulose, and cellulose, but no apparent correlation with pH, EC, GI, and lignin. Results reported here may provide an efficient way to save energy and investment in the industry production of pellet compost.
Indoor experiments were performed to determine the accumulation, subcellular distribution, and chemical forms of Cd at five S levels in Hydrilla verticillata. The Cd content increased from 1.229 mg/g to 3.329 mg/g in leaves, and decreased from 2.794 mg/g to 1.023 mg/g in roots, respectively. Excess S supply stimulated Cd assimilation in leaves as Cd accumulation was inhibited in roots. The Cd content in leaves at subcellular levels revealed that Cd was stored mainly in the soluble fraction (71.9-88.2%), and in small quantities in the cell wall (6.1-22.4%) and cell organelles (4.8-6.9%). As S increased, the Cd content in leaf soluble fractions and cell walls increased remarkably. The content of NaCl-extracted Cd in leaves increased as S supply increased, and this parameter was much higher than that of other Cd forms. In leaves, the Cd concentrations in the cell walls were significantly correlated with the chemical forms extracted by HAc, HCl, and NaCl, with correlation coefficients of 0.985, 0.964, and 0.957, respectively. The high correlation indicated that Cd in soluble fractions or cell walls was mainly in the form of pectates/protein, phosphate, and oxalate. The application of S alleviated Cd-induced oxidative stress by increasing the proline accumulation. Furthermore, sulfhydryl proteins such as glutathione and cysteine may play a crucial role in the reversal of Cd-induced oxidative stress.
With the liquid crystal displays (LCDs) being widely used in televisions, notebooks, and mobile phones, etc., large quantities of LCDs are entering into their end-of-life stage for treatment. If not treated properly, a loss of resources and undesirable impacts on the environment and human health can occur. In order to treat the waste LCDs in an efficient and environmentally friendly way, a combined process of physical methods was proposed to separate and recover materials from waste LCDs in the present study. On the basis of primary disassembly, two key processes (including liquid crystals removal and the recovery of polarizer and glass) were studied. Liquid crystals were removed from the panel glass by dissolving in isopropyl alcohol solution (16.7 vol.%) assisted with ultrasound. Recovery of polarizer and glass was achieved through mechanical crushing and gravity concentration. Results show that approximately 100 wt.% of liquid crystals were removed after dissolving for 45 min at 60ºC. Up to 79.7 wt.% of polarizer was separated from glass and its average content in the recovered product was 90.3 wt.%.
Nutrition plays a key role in the maintenance of animal reproductive performance. Reduced reproductive efficiency can decrease the profitability of dairy production by increasing days open, calving interval, the number of services per conception, culling rate, and veterinary services. Nutritional requirements increase rapidly with milk production after calving, but an improper diet plan could result in a negative energy balance (NEB). NEB delays the time of first ovulation through uncoupled hormones production. A diet high in fat could prevent the NEB state by increasing the energy status of animals. Protein supplementation supports high production but can also have severe effects on the reproductive performance of the animal. Deficiency of minerals can also alter the reproductive performance of the animal. This article has generally focused on the effects of various nutrients on reproductive efficiency of dairy cattle.
While simultaneous processing of heavy metal-rich waste and municipal sewage sludge (MSS) in cement kilns may be a useful measure for protecting the environment, simultaneous co-disposal of these two types of wastes has seldom been reported in detail. In this study, we examined how MSS influenced the fixation of heavy metals during clinkerization and determined the main controlling mechanisms through analyzing the polymorphism of tricalcium silicate (C₃S), mineral composition of MSS, and element distribution in the clinker. The results showed that MSS had negative effects on the fixation of heavy metals, with reductions of 12.9%, 8.7%, 3.2%, and 1.2% in the amounts of Cu, Ni, Cr, and Zn fixed, respectively. These changes were mainly attributed to the presence of trace elements – in particular phosphorus from the MSS, which caused the polymorphism of C₃S to change in the order: rhombohed ral→monoclinic→triclinic. As well as occurring as C₂S-C₃P, phosphorus also occurred in new phases of K₂NiP₂O₇, K₂Cu(PO₃)₄, and Cu₄O(PO₄)₂ in cement clinker. In general, trace elements from the MSS, especially phosphorus, decreased the solubility of heavy metals in cement clinker through changing the polymorphism of C₃S, and resulting in a decrease in the fixation ratios of heavy metals.
Background: Laryngeal cancer tends to have a very poor prognosis due to the unsatisfactory efficacy of chemotherapy for this cancer. Multidrug resistance (MDR) is the main cause of chemotherapy failure. The proto-oncogene c-fos has been shown to be involved in the development of MDR in several tumor types, but few studies have evaluated the relationship between c-fos and MDR in laryngeal cancer. We investigated the role of c-fos in MDR development in laryngeal cancer cells (cell line: human epithelial type 2, HEp-2) using the chemotherapeutic vincristine (VCR). Methods: HEp-2/VCR drug resistance was established by selection against an increasing drug concentration gradient. The expressions of c-fos and multidrug resistance 1 (mdr1) were measured using qPCR and western blot. C-fos overexpression or knockdown was performed in various cells. The intracellular rhodamine-123 (Rh-123) accumulation assay was used to detect the transport capacity of P-glycoprotein (P-gp, which is encoded by the mdr1 gene). Results: HEp-2 cells with VCR-induced resistance (HEp-2/VCR cells) were not only resistant to VCR but also evolved cross-resistance to other chemotherapeutic drugs. The expressions of the c-fos and mdr1genes were significantly higher in the HEp-2/ VCR cells than in control cells. C-fos overexpression in HEp-2 cells (c-fos WT) resulted in increased P-gp expression and increased the IC50 for 5-FU. C-fos knockdown in the HEp-2/VCR cells (c-fos shRNA) resulted in decreased P-gp expression and decreased IC50 for 5-FU. An intracellular Rh-123 accumulation assay showed that the mean intracellular fluorescence intensity (MFI) was lower in the HEp-2/VCR cells than in HEp-2 cells. C-fos WT cells also showed lower MFI. By contrast, c-fos shRNA cells exhibited a higher MFI than the control group. Conclusion: C-fos increased the expression of P-gp and mdr1 in the HEp-2/VCR cells, and enhanced the efflux function of the cells, thereby contributing to the development of MDR.
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