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Kamica szczawianowo-wapniowa u psów i kotów

100%

Wylupek D., Madany J.

Życie Weterynaryjne

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2012

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tom 87

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nr 04

2

Przydatność techniki LAMP w szybkim rozpoznawaniu babeszjozy u zwierząt

81%

Adaszek L., Jankowska M., Wylupek D., Winiarczyk S.

Medycyna Weterynaryjna

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2013

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tom 69

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nr 03

Nucleic acid-based methods offer a variety of tools for the detection of parasites. This field of veterinary and medical sciences is rapidly evolving. Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid method whereby DNA is amplified with high specificity, efficiency, and rapidity under isothermal conditions using a set of four specifically designed primers. In this paper we present the usefulness of LAMP method in the diagnosis of babesiosis in various animal species. Babesia species-specific LAMP assay may have potential clinical application in the detection and differentiation of Babesia species, particularly in countries in which babesiosis is endemic. It can be assumed that in the near future this technique will be a routinely used diagnostic test in veterinary practices.

3

Przydatność barwionego rozmazu krwi we wczesnej diagnostyce sepsy

71%

Milczak A., Madany J., Adaszek L., Surma-Kurusiewicz K., Wylupek D.

Magazyn Weterynaryjny

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2014

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tom 23

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nr 11

4

Applicability of 2D gel electrophoresis and liquid chromatography in proteomic analysis of urine using mass spectrometry MALDI-TOF

71%

Banach T., Adaszek L., Wylupek D., Winiarczyk M., Winiarczyk S.

Polish Journal of Veterinary Sciences

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2013

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tom 16

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nr 3

Proteomics including the studies of the structure, function and dependences between proteins is more and more extensively applied in human medicine and veterinary medicine. The analysis of protein profiles of tissues and body fluid from healthy and ill individuals allows to identify diagnostic, prognostic and predictive markers in various pathological states in people and animals. This paper presents preparation of urine samples for analysis in the mass spectrometer MALDI-TOF (Ultraflextreme, Bruker, Bremen, Germany) by means of two methods: liquid chromatography based on the system Nano-LC (PROTEINER FC II, Bruker Daltonics, Bremen Germany). and two-direction electrophoresis 2DE (GE Healthcare, United Kingdom). Both methods enable separation of the mixture under consideration into individual fractions of high purity indispensable for obtaining readable mass spectra. The purpose of this paper is to determine applicability of these methods in analysis of protein composition of urine samples.

5

Detection of Borrelia burgdorferi sensu lato DNA in the blood of wild bison from Bialowieza Primeval Forest in eastern Poland

51%

Adaszek L., Dziegiel B., Krzysiak M., Skrzypczak M., Adaszek M., Staniec M., Wylupek D., Winiarczyk S.

Polish Journal of Veterinary Sciences

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2014

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tom 17

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nr 4

The aim of the present study was to investigate the occurrence of Borrelia burgdorferi sensu lato DNA in a group of 120 wild bison (Bison bonasus) from the Bialowieza Primeval Forest in eastern Poland. The PCR technique revealed the presence of 16S RNA of Borrelia burgdorferi sensu lato in the blood of 16 out of 120 examined animals. DNA amplification by means of primers SC1 and SC2 gave a product with a size of 300-bp. The sequences of the PCR products obtained showed 100% homology with each other and 100% homology with B. burgdorferi s.l. 16S RNA gene DQ111061. Results of this study suggest that wild bison are important in maintaining agents of Lyme borreliosis, and that studies of reservoir competence of this species are indicated.

6

Application of rhinoscopy in the diagnostics of nasal tumors in dogs

51%

Adaszek L., Staniec M., Buczek K., Lopuszynski W., Smiech A., Milczak A., Debiak P., Klimiuk P., Wylupek D., Winiarczyk S.

Medycyna Weterynaryjna

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2014

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tom 70

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nr 01

The aim of this article was to describe cases of nasal tumors in dogs in which a rhinoscopy procedure was used as part of the process of disease diagnosis. The study included two dogs, aged 8 and 11 years, showing symptoms of epistaxis. The animals underwent a radiological examination and a rhinoscopy, during which bioptats were taken for histopathological examination. The radiological examination of the head did not reveal lesions characteristic of a neoplastic process. The rhinoscopic examinations showed a large hyperplasia closing the nasal canal in both dogs. The histopathological examination of the two bioptats sampled from the nose area demonstrated clusters of cells characteristic of a neoplastic process. The dogs were euthanized and subjected to a post-mortem examination. The histopathological examination of samples taken from the lesions in the nasal cavity confirmed olfactory neuroblastoma and transitional cell carcinoma in dogs 1 and 2, respectively. Rhinoscopy is a technique complementary to computer tomography, and, if the latter is impossible, it should represent, along with a radiological examination, the basis for a preliminary diagnosis of a neoplastic process, which ought to be confirmed by a cytological or histopathological examination of bioptats obtained from the sites of the lesions.

7

Prevalence of Babesia canis, Borrelia burgdorferi sensu lato, and Anaplasma phagocytophilum in hard ticks collected from meadows of Lubelskie Voivodship (eastern Poland)

51%

Dziegiel B., Kubrak T., Adaszek L., Debiak P., Wylupek D., Bogucka-Kocka A., Lechowski J., Winiarczyk S.

Bulletin of the Veterinary Institute in Puławy

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2014

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tom 58

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nr 1

The aim of the study was to assess the distribution of Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, and Babesia canis in adult females and males of Ixodes ricinus and Dermacentor reticulatus ticks, inhabiting meadows near large forest complexes throughout the Lubelskie Voivodship (eastern region of Poland). Ticks were collected using the flagging method. Among 720 ticks collected, 506 were identified as D. reticulatus, and 214 as I. ricinus. DNA of B. canis and B. burgdorferi s.l. was detected in 21.3% and 0.6% of D. retiadatus ticks, respectively. In I. ricinus ticks, DNA specific to B. burgdorferi s.l. and A. phagocytophilum was detected in 5.6% and 10.3%, respectively. Co-infections of B. burgdorferi s.l. and A. phagocytophilum were found in two I. ricinus ticks. These results indicate that the Lublin region is an area at risk of tick-borne diseases of humans and animals, which must be considered in clinical practice.

Ograniczanie wyników

2 Medycyna Weterynaryjna

2 Polish Journal of Veterinary Sciences

1 Bulletin of the Veterinary Institute in Puławy

1 Magazyn Weterynaryjny

1 Życie Weterynaryjne

7 Wylupek D.

6 Adaszek L.

5 Winiarczyk S.

2 Debiak P.

2 Dziegiel B.

2 Madany J.

2 Milczak A.

2 Staniec M.

1 Adaszek M.

1 Banach T.

1 Bogucka-Kocka A.

1 Buczek K.

1 Jankowska M.

1 Klimiuk P.

1 Krzysiak M.

1 Kubrak T.

1 Lechowski J.

1 Lopuszynski W.

1 Skrzypczak M.

1 Smiech A.

1 Surma-Kurusiewicz K.

1 Winiarczyk M.

4 2014

2 2013

1 2012

Kamica szczawianowo-wapniowa u psów i kotów

Przydatność techniki LAMP w szybkim rozpoznawaniu babeszjozy u zwierząt

Przydatność barwionego rozmazu krwi we wczesnej diagnostyce sepsy

Applicability of 2D gel electrophoresis and liquid chromatography in proteomic analysis of urine using mass spectrometry MALDI-TOF

Detection of Borrelia burgdorferi sensu lato DNA in the blood of wild bison from Bialowieza Primeval Forest in eastern Poland

Application of rhinoscopy in the diagnostics of nasal tumors in dogs

Prevalence of Babesia canis, Borrelia burgdorferi sensu lato, and Anaplasma phagocytophilum in hard ticks collected from meadows of Lubelskie Voivodship (eastern Poland)