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1

Characterization and identification of gut-associated phytase-producing bacteria in some fresh water fish cultured in ponds

100%

Khan A., Ghosh K.

Acta Ichthyologica et Piscatoria

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2012

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tom 42

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nr 1

Background. Phytase produced by gut bacteria increases the availability of phosphorus and other important nutrients in ruminants by virtue of enzymatic hydrolysis of the phytic acid, an antinutritional factor present in the majority of plant feedstuffs. This topic, however, has been insufficiently investigated in fish. This study was intended: to evaluate the presence of phytase-producing autochthonous bacteria in the gastrointestinal (GI) tracts of 14 freshwater teleost fishes; and to identify the most promising phytase-producing strains by phenotypic characterization and 16S rDNA. Materials and Methods. The GI tracts were removed and divided into proximal (PI) and distal (DI) intestine. Homogenates of intestinal segments were spread onto sterilized tryptone soya agar and modified phytase screening media (MPSM) plates to determine autochthonous culturable heterotrophic and phytase-producing microbiota, respectively. Data were presented as log viable counts (LVC) g-1 intestine. Out of 95 phytase-producing isolates, primarily selected 32 isolates were studied for phytase-assay using MPSM broth. Promising phytase-producing isolates were evaluated for other exo-enzymes (amylase, cellulase, protease, lipase) using 4 selective media. Two most promising phytase-producing isolates were identified by phenotypic characterization and 16S rDNA. Results. Population of heterotrophic bacteria was highest (LVC = 8.29 g-1 intestine) in the DI of Gudusia chapra followed by DI of Hypophthalmichthys molitrix (LVC = 6.82 g-1 intestine). However, more than log 4 reduction of the phytase-producing bacteria was observed compared to heterotrophic bacteria. Phytase-producing microbiota was highest in the PI of G. chapra (LVC = 3.95 g-1 intestine) followed by PI of Labeo calbasu (LVC = 3.78 g-1 intestine). The strain LB1.4 isolated from DI of Labeo bata showed highest phytase activity (2.33 ± 0.006 U · mL–1) followed by the strain GC1.2 (2.19 ± 0.018 U · mL–1) isolated from PI of G. chapra. Both isolates were efficient in producing other exo-enzymes. Phenotypic characterization and nucleotide homology analysis revealed that the isolates LB1.4 and GC1.2 were similar to Bacillus subtilis and Bacillus atrophaeus, respectively. Conclusion. Autochthonous phytate degrading bacteria were present in the GI tract of fish that might endow ecological advantages to overcome the anti-nutritional effects of plant phytate.

2

Effects of four host plants on biological parameters of Maconellicoccus hirsutus Green [Homoptera: Pseudococcidae] and efficacy of Anagyrus kamali Moursi [Hymenoptera: Encyrtidae]

100%

Persad A., Khan A.

Journal of Plant Protection Research

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2007

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tom 47

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nr 1

An evaluation of the effect of four host plants on biological parameters of Maconellicoccus hirsutus and efficacy of Anagyrus kamali was conducted in the laboratory. Hibiscus rosa-sinensis and Hibiscus sabdariffa produced M. hirsutus with life cycles which were 1-2 days shorter than those of M. hirsutus reared on Solanum tuberosum, and Cucurbita pepo L. Egg to adult M. hirsutus survival was lowest in C. pepo (8.8%) and highest for H. sabdariffa (21.8%). C. pepo, H. rosa-sinensis and H. sabdariffa produced a more female biased M. hirsutus sex ratio from single ovisacs, than S. tuberosum. Although adult M. hirsutus females exhibited variation in size and longevity on the host plants tested, total fecundity was not significantly different. Life cycle of both sexes and offspring sex ratio of A. kamali emerging from M. hirsutus cultured on the four host plants were statistically similar. M. hirsutus infested H. rosa-sinensis and H. sabdariffa produced A. kamali with significantly higher fecundity (post emergence adult counts) and lower longevity compared to the other host plants. Females with largest femur (Mean length = 0.2950 ± 0.0053 mm) were produced by M. hirsutus infested H. rosa-sinensis. A. kamali efficacy measured by percent parasitization and percent adult eclosion was statistically similar for all M. hirsutus infested host plants tested.

3

Disrupted brain thyroid hormone homeostasis and altered thyroid hormone-dependent brain gene expression in autism spectrum disorders

81%

Khan A., Harney J. W., Zavacki A. M., Sajdel-Sulkowska E. M.

Journal of Physiology and Pharmacology

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2014

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tom 65

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nr 2

4

On the long-term variability of temperature trends and changes in surface air temperature in Kolkata Weather Observatory, West Bengal, India

81%

Khan A., Chatterjee S., Bisai D.

Meteorology Hydrology and Water Management. Research and Operational Applications

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2015

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tom 03

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nr 2

The impact of climate change on annual air temperature has received a great deal of attention from climatologists worldwide. Many studies have been conducted to illustrate that changes in temperature are becoming evident on a global scale. Air temperature, one of the most important components of climate parameters, has been widely measured as a starting point towards the apprehension of climate change and variability. The main objective of this study is to analyse the temporal variability of mean monthly temperature for the period of 1941 to 2010 (70 years). To detect the magnitude of trend in mean monthly temperature time series, we have used non-parametric test methods such as The Mann-Kendall test, often combined with the Theil-Sen’s robust estimate of linear trend. Whatever test is used, the user should understand the underlying assumptions of both the technique used to generate the estimates of a trend and the statistical methods used for testing. The results of this analysis reveal that four months – January, February, March and December – indicate a decreasing trend in average temperature, while the remaining eight months have an increasing trend. The magnitude of Mann-Kendall trend statistic Zc for this declining temperature and the magnitude of slope for the months of January, February and December are confirmed at the high significance levels of α = 0.001, 0.01 and 0.1 respectively. Though, the overall trend is positive for monthly as well as seasonally efficient time series.

5

Functional characterization of human Kindlin-2 core promoter identifies a key role of SP1 in Kindlin-2 transcriptional regulation

81%

Khan A., Shimokawa T., Stromblad S., Zhang H.

Cellular and Molecular Biology Letters

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2011

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tom 16

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nr 4

Kindlin-2 is a recently identified FERM and PH domain containing integrin interacting protein. Kindlin-2 is ubiquitously expressed in normal tissues. So far, much effort has been spent exploring the functional aspects of Kindlin-2. However, the transcriptional regulation of Kindlin-2 has not yet been investigated. In this study we identified and functionally characterized the promoter of the human Kindlin-2 gene. We show that the core promoter of Kindlin-2 is a 39 base pair long GC rich fragment located −122/-83 upstream of the Kindlin-2 transcription start site. Functional characterization of this core promoter region by both in silico as well as in vitro/in vivo analysis shows that the transcription factor SP1 plays an important role in regulation of Kindlin-2 expression.

6

The interaction of metal nanoparticles (copper, silver, platinum, and gold) with cell line HS-5

81%

Lange A., Jaworski S., Khan A.

Folia Biologica

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2020

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tom 68

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nr 3

7

Salicylic acid mitigates salinity stress by improving antioxidant defence system and enhances vincristine and vinblastine alkaloids production in periwinkle [Catharanthus roseus (L.)G. Don]

71%

Idrees M., Naeem M., Aftab T., Masroor M., Khan A.

Acta Physiologiae Plantarum

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2011

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tom 33

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nr 3

A pot experiment was conducted to find out whether the foliar spray of salicylic acid (SA) could successfully ameliorate the adverse effects of salinity stress on periwinkle. Thirty-day-old plants were supplied with Control; 0 mM NaCl + 10⁻⁵ M SA (T₁); 50 mM NaCl + 0 SA (T₂); 100 mM NaCl + 0 SA (T₃); 150 mM NaCl + 0 SA (T₄); 50 mM NaCl + 10⁻⁵ M SA (T₅); 100 mM NaCl + 10⁻⁵ M SA (T₆); 150 mM NaCl + 10⁻⁵ M SA (T₇). The plants were sampled 90 days after sowing to assess the effect of SA on stressed and unstressed plants. Salt stress significantly reduced the growth attributes including plant height, leaf-area index, shoot and root fresh weights, shoot and root dry weights. Increasing NaCl concentrations led to a gradual decrease in photosynthetic parameters and activities of nitrate reductase and carbonic anhydrase. Ascorbic acid, total alkaloids and antioxidants enzymes superoxide dismutase, catalase and peroxidase also declined in NaCl-treated plants. The plants, undergoing NaCl stress, exhibited a significant increase in electrolyte leakage and proline content. Foliar application of SA (10⁻⁵ M) reduced the damaging effect of salinity on plant growth and accelerated the restoration of growth processes. It not only improved the growth parameters but also reversed the effects of salinity. Total alkaloid content was improved by SA application both in unstressed and stressed plants. The highest level of total alkaloid content recorded in leaves of SA-treated stressed plants was 11.1%. Foliar spray of SA overcame the adverse effect of salinity by improving the content of vincristine (14.0%) and vinblastine (14.6%) in plants treated with 100 M NaCl.

8

Sex-dependent changes in cerebellar thyroid hormone-dependent gene expression following perinatal exposure to thimerosal in rats

71%

Khan A., Sulkowski Z. L., Chen T., Zavacki A. M., Sajdel-Sulkowska E. M.

Journal of Physiology and Pharmacology

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2012

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tom 63

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nr 3

9

Increase in the effectiveness of somatodendritic 5-HT-1A receptors in a rat model of tardive dyskinesia

61%

Samad N., Khan A., Perveen T., Haider S., Haleem M. A., Haleem D. J.

Acta Neurobiologiae Experimentalis

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2007

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tom 67

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nr 4

The present study concerns responsiveness of pre- and postsynaptic 5-hydroxytryptamine (5-HT)-1A receptors in a rat model of tardive dyskinesia (TD). Vacuous chewing movements (VCMs) in rats are widely accepted as an animal model of TD. Results show that haloperidol injected at a dose of 1 mg/kg twice a day for 5 weeks elicited VCMs, which increased in a time dependent manner following the drug administration for 3Ė5 weeks. Tolerance was produced in motor coordination during the potentiation of VCMs. Exploratory activity in an open field and in an activity box decreased in haloperidol treated animals. The effects of 8-hydroxy-2-(di-n-propylamino)tetraline (8-OH-DPAT; 0.5 mg/kg) were monitored 48-h after withdrawal from repeated administration of haloperidol. 8-OH-DPAT-induced locomotion was greater in haloperidol treated rats. 5-HT synthesis increased in haloperidol treated animals, while 8-OH-DPAT-induced decreases of 5-HT synthesis were greater in repeated haloperidol than repeated saline injected animals. The results suggest that an increase in the effectiveness of somatodendritic 5-HT-1A receptors may decrease the inhibitory influence of 5-HT on the activity of dopaminergic neurons to precipitate VCMs. The 5-HT-1A agonist may help to alleviate neuroleptic-induced TD.

10

Mitigation of As toxicity in wheat by exogenous application of hydroxamate siderophore of Aspergillus origin

61%

Kumari S., Khan A., Singh P., Dwivedi S. K., Ojha K. K., Srivastava A.

Acta Physiologiae Plantarum

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2019

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tom 41

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nr 07

Siderophores are secondary metabolites having molecular weight less than 10 KD. They are specifically meant for chelation of ferric ions. They also tend to chelate metals under heavy metal stress, thus reducing their toxic effects. In the current study, experiments have been conducted on wheat plants to analyse siderophore’s ability to counteract the adverse impact of arsenic (As) toxicity on physiology of plant seedlings along with biochemical response. As toxicity has been observed to adversely affect the lengths of root and shoot, chlorophyll and carotenoid contents, and activities of various antioxidative enzymes. The present study revealed that the application of hydroxamate-type siderophore isolated from Aspergillus nidulans under toxic condition significantly recovered the growth and helped in amending the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) of wheat genotype (NW1014). At the same time, injury caused by lipid peroxidation was significantly reduced. In silico studies revealed better binding affinity of ferricrocin–arsenate complex leading to thermodynamically stable complex. Encouraging results of As containment by organic biomolecule-siderophore can lead to an emerging bioremediation mechanism brimming with opportunities for agricultural field and environmental clean-up.

11

Calcium chloride and gibberellic acid protect linseed (Linum usitatissimum L.) from NaCl stress by inducing antioxidative defence system and osmoprotectant accumulation

61%

Khan M. N., Siddiqui M. H., Mohammad F., Naeem M., Masroor M., Khan A.

Acta Physiologiae Plantarum

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2010

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tom 32

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nr 1

Salinity stress affects many metabolic facets of plants and induces anatomical and morphological changes resulting in reduced growth and productivity. To overcome the damaging effects of salinity, different strategies of the application of nutrients with plant hormones are being adopted. The present study was carried out with an aim to find out whether application of calcium chloride (CaCl₂) and gibberellic acid (GA₃) could alleviate the detrimental effects of salinity stress on plant metabolism. Fifteen days old plants were supplied with (1) 0 mM NaCl + 0 mg CaCl₂ kg⁻¹ sand + 0 M GA₃ (control, T0); (2) 0 mM NaCl + 10 mg CaCl₂ kg⁻¹ sand + 0 M GA₃ (T1); (3) 0 mM NaCl + 0 mg CaCl₂ kg⁻¹ sand + 10⁻⁶ M GA₃ (T2); (4) 150 mM NaCl + 0 mg CaCl₂ kg⁻¹ sand + 0 M GA₃ (T3); (5) 150 mM NaCl + 10 mg CaCl₂ kg⁻¹ sand + 0 M GA₃ (T4); (6) 150 mM NaCl + 0 mg CaCl₂ kg⁻¹ sand + 10⁻⁶ M GA₃ (T5); (7) 150 mM NaCl + 10 mg CaCl₂ kg⁻¹ sand + 10⁻⁶ M GA₃ (T6). To assess the response of the crop to NaCl, CaCl₂ and GA₃, plants were uprooted randomly at 60 days after sowing. The presence of NaCl in the growth medium decreased all the growth and physio-biochemical parameters, except electrolyte leakage, proline (Pro) and glycine betaine (GB) content, thiobarbituric acid reactive substances (TBARS), H₂O₂ content, activities of superoxide dismutase (SOD) and catalase (CAT) and leaf Na content, which exhibited an increase of 37.6, 29.3, 366.9, 107.5, 59.1, 17.1, 28.4 and 255.2%, respectively, compared to the control plants. However, application of CaCl₂ in combination with GA₃ appears to confer greater osmoprotection by the additive role with NaCl in Pro and GB accumulation. Although the activities of antioxidant enzymes (SOD, CAT and POX) were increased by salt stress, the combined application of CaCl₂ and GA₃ to salt-stressed plants further enhanced the activities of these enzymes by 25.1, 6.7 and 47.8%, respectively, compared to plants grown with NaCl alone. The present study showed that application of CaCl₂ and GA₃ alone as well as in combination mitigated the adverse effect of salinity, but combined application of these treatments proved more effective in alleviating the adverse effects of NaCl stress.

12

Retrospective study of cystic echinococcosis (CE) based on hospital record from five major metropolitan cities of Pakistan

61%

Muqaddas H., Arshad M., Ahmed H., Mehmood N., Khan A., Simsek S.

Acta Parasitologica

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2019

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tom 64

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nr 4

13

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Potential of chitosan alone and in combination with agricultural wastes against the root-knot nematode, Meloidogyne incognita infesting eggplant

61%

Asif M., Ahmad F., Tariq M., Khan A., Ansari T., Khan F., Siddiqui A. M.

Journal of Plant Protection Research

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2017

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tom 57

|

nr 3

The present investigation was carried out to evaluate the potential of chitosan alone and in combination with various agricultural wastes for the management of rootknot nematode, Meloidogyne incognita on eggplant cv. ‘BR-112’ under greenhouse conditions. The results showed that chitosan as a single or joint treatment with agricultural wastes significantly (p ≤ 0.05) reduced root-knot indices, and the nematode population in soil. As a result, of this, the growth and growth yielding attributes of eggplant were remarkably augmented. Chitosan as an elicitor induced plant mediated systemic resistance against M. incognita in eggplant. The results of the study demonstrated that maximum reduction in eggmass/root, eggs/eggmasses, nematode population and root-knot indices, was acquired by the treatments: chitosan + onion and chitosan + mentha. It was followed by chitosan + Brassica, chitosan + urad and chitosan + coconut whereas, chitosan combined with corn cob waste was found to be the least effective when compared to the control. The application of chitosan alone was effective but not very satisfactory. Compared to the control applications of all the treatments significantly increased plant growth in terms of length, fresh and dry weights, pollen fertility, yield and biochemical parameters such as chlorophyll, carotenoid content and antioxidant enzymes. This may have been due to the eliciting activity of chitosan, causing systemic resistance in the plant and the release of various toxic chemical compounds during decomposition which have lethal effects against the second stage juveniles of M. incognita and nematode multiplication.

14

Characterizing microbial populations in petroleum-contaminated soils of Swat district, Pakistan

52%

Uddin M. N., Ali M., Muhammad M., Farooq M., Ahmad N., Jamil J., Kalsoom J. J., Adnan M., Shah N., Khan A.

Polish Journal of Environmental Studies

|

2016

|

tom 25

|

nr 4

Soil samples from petroleum-contaminated soil were collected from 25 different petroleum filling stations and automobile workshops in the district of Swat, Khyber Pakhtunkhawa, Pakistan. A total of seven bacterial genera were isolated. All of the isolates were Gram-positive bacteria. The genera identified by the culture and cell morphological characteristics were: Bacillus, Streptococcus, Staphylococcus, Micrococcus, Corynebacterium Arthrobacter, and Streptomyces. Lipolytic and saline activities of the selected isolates were studied. Among the isolates, Arthrobacter, Staphylococcus, Bacillus, Micrococcus, Corynebacterium, and Streptomyces produced lipase enzymes, while no lipase was produced by Streptococcus. Dense growth of Bacillus and Streptococcus was observed at 1% NaCl. Dense growth of Streptomyces was observed at strength of 2% NaCl. At 3% NaCl concentration, dense growth of Staphylococcus, Micrococcus, Corynebacterium, and Arthrobacter was observed, indicating that they were moderately halotolerent. In our study, Bacillus, Arthrobacter, and Streptomyces showed optimum growth at pH 8.0, and Streptococcus, Staphylococcus, and Micrococcus showed optimum growth at pH 7.0. Only Corynebacterium showed optimum growth at pH 9.0, indicating that it is tolerant of higher pH conditions.

15

Quantitative determination of lethal concentration Lc50 of atrazine on biochemical parameters; total protein and serum albumin of freshwater fish grass carp (Ctenopharyngodon idella)

52%

Khan A., Shah S., Muhammad N., Khan M. S., Ahmad M. S., Farooq M., Adnan M., Jawad S. M., Ullah H., Yousafzai A. M.

Polish Journal of Environmental Studies

|

2016

|

tom 25

|

nr 4

The indiscriminate use of herbicides has led to the contamination of water bodies, possibly affecting the health of aquatic biota, especially fish. Atrazine is considered as toxicants for aquatic fauna, due to its high persistence in soil, high half-life and high mobility toward aquatic bodies as well as high solubility in water. The objective of the present study was to determine (LC50) and to evaluate the acute and chronic toxicity of atrazine on the biochemical parameters; total protein and serum albumin of freshwater grass carp (Ctenopharyngodon idella). Above 15 μlL⁻¹, the LC50 was recorded revealing sensitivity of grass carp to atrazine. Grass carp was exposed to atrazine for 01 (15 μlL⁻¹), 02 (13 μlL⁻¹), 03 (10 μlL⁻¹), and 04 (08 μlL⁻¹) days/concentration for scrutinizing acute toxicity. Likewise, fish were exposed to atrazine for 10 (06 μlL⁻¹), 15 (04 μlL⁻¹), and 25 (02 μlL⁻¹) days/concentration for scrutinizing chronic toxicity. Control group concentration was 8.3 gL⁻¹ and 3.5 gL⁻¹. Total protein concentration observed for acute toxicity was 7.5 gL⁻¹, 6.5 gL⁻¹, 4.6 gL⁻¹, and 3.2 g/L and serum albumin concentration was 2.7 gL⁻¹, 1.6 gL⁻¹, 1.4 gL⁻¹, and 1.1 gL⁻¹ respectively. Similarly total protein concentration observed for chronic toxicity was 8.2 gL⁻¹, 6.8 gL⁻¹, and 4.3 gL⁻¹ and serum albumin concentration was 2.1 gL⁻¹, 1.7 gL⁻¹, and 1.4 gL⁻¹ respectively. Markedly decline (denoted by P<0.05, P≤0.01 and P≤0.001) was noticed in both the parameters concentration during acute as well as chronic toxicity, when compared with control group concentration, indicating negatively impinge of atrazine on grass carp as well as atrazine present in aquatic bodies must jeopardize the health of other aquatic fauna.

16

Effect of heavy metal pollution on mineral absorption in sunflower (Helianthus annuus L.) hybrids

52%

Aslam U., Ahmad I., Hussain M., Khan A., Ghani A., Mustafa I., Jalal S., Aqeel M. A., Asif S., Ahmed H.

Acta Physiologiae Plantarum

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2014

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tom 36

|

nr 01

The present study was conducted in a potted experiment to examine the effects of chromium pollution on absorption of mineral nutrients and some morphophysiological attributes of two sunflower (Helianthus annuus L.) hybrids (FH-331 and FH-259) in the presence and absence of ethylene diamine tetra acetic acid (EDTA) used as a chelating agent. Four concentrations of chromium (Cr³⁺) i.e., 0, 20, 30 and 40 mg kg⁻¹ with and without 0.3 g kg⁻¹ , EDTA as chelating agent were applied to 25-day-old sunflower plants. A gradually decreasing trend in absorption of all minerals and other parameters studied were observed. Different treatments of Cr³⁺ as well as Cr³⁺ and EDTA significantly reduced root and shoot fresh weight; however, root, shoot and achene Cr³⁺ contents of two sunflowers hybrids under higher chromium and EDTA stress varied significantly whereas movement of Cr³⁺ contents to leaves was non-significant. Absorption of Na⁺, K⁺, N₂ and P through roots and shoots significantly reduced with increasing concentration of Cr³⁺ treatments. In fact addition of EDTA to the medium further enhanced the toxicity of chromium.

17

Different tolerance of photosynthetic apparatus to Cd stress in two rice cultivars with the same leaf Cd accumulation

52%

Chen Y.-E., Mao H.-T., Wu N., Khan A., Din A. M. U., Ding C.-B., Zhang Z.-W., Yuan S., Yuan M.

Acta Physiologiae Plantarum

|

2019

|

tom 41

|

nr 12

In plants, cadmium (Cd) is regarded as one of the most toxic metals and affects many physiological and biochemical processes. To investigate the effects of Cd on photosynthesis and antioxidant system of japonica and indica rice cultivars, Wuyu 21 (WY21) and IIyou 808 (IIY808) seedlings were exposed to different concentrations of Cd for 7 days. Our results indicated that Cd treatments resulted in the significant decrease in photosynthetic capacity and the obvious oxidative damage in WY21 and IIY808. Although Cd contents in the leaves and stem had no obvious difference between WY21 and IIY808 under Cd stress, japonica cultivar WY21 showed higher Cd contents of roots and photosynthetic efficiency compared with indica cultivar IIY808 under Cd stress. In contrast, the lower generation of reactive oxygen species (ROS) and cell death were observed in WY21 relative to IIY808. However, almost all antioxidant enzymes activities and the concentrations of four antioxidants showed no significant differences between WY21 and IIY808. Furthermore, the severe oxidative damage in IIY808 was accompanied by the marked decline in the levels of two photosystem II (PSII) proteins (D2 and D1) under high concentration of Cd. In conclusion, we concluded that high Cd resistance in japonica cultivar WY21 is probably attributed to the high photosynthesis under Cd stress.

18

Comparative study of toxicological impinge of glyphosate and atrazine (herbicide) on stress biomarkers; Blood biochemical and hematological parameters of the freshwater common carp (Cyprinus carpio)

52%

Khan A., Shah N., Gul A., Us Suhar N., Ismail A., Muhamad, Aziz F., Farooq M., Adnan M., Rizwan M.

Polish Journal of Environmental Studies

|

2016

|

tom 25

|

nr 5

The purpose of the present study was to scrutinize the effect of glyphosate and atrazine (herbicide) on blood biochemical and hematological parameters of common carp, (Cyprinus carpio), including plasma glucose (RBS), cholesterol (CH), serum protein (SP), creatinine phosphates (CPK), lactate dehydrogenase (LDH), WBC, hemoglobin (Hb), platelets (PT), lymphocytes (LP), monocytes (MT), esinophils (EN), and neutrophils (NT), and on behavioral aspects for (24, 48, 72, 96) hours under doses of (0.1, 0.07, 0.05, 0.02) and (0.2, 0.15, 0.1, 0.05)mlL-1 respectively of glyphosate and atrazine. For analysis of biochemical and hematological parameters, the protocol of biochemical analyzer set (Merck Micro Lab 300 biochemistry analyzer) and hematological analyzer (Mindray BC-2300 Hematology Analyzer) was followed in the laboratory. An upturn in RBS, CH, and WBC concentration was observed while SP, LDH, LT, MT, and EP concentrations were decreased against both herbicides. CPK and Hb concentrations were increased against atrazine, while against glyphosate the concentrations were decreased. PT and NT showed momentous upturn in concentrations against glyphosate, while showing a decline against atrazine. Both herbicides affected the blood biochemical and hematological parameters of the selected fish. Behaviorally, changes were observed against both herbicides, including loss of equilibrium, increase in the frequency of opercular movements, fast swimming and jumping, losing balance, becoming exhausted and lethargic, vertical swimming, and bleeding at the base of the eyeballs.

19

Potential of endochitinase gene to control Fusarium wilt and early blight disease in transgenic potato lines

52%

Fatima N., Tabassum B., Yousaf I., Malik M., Khan A., Sajid I. A., Tariq M., Toufiq N., Riaz S., Nasir I. A.

Journal of Plant Protection Research

|

2019

|

tom 59

|

nr 3

20

Enzymatic profile aactivity of grass carp (Ctenopharyngodon idella) after exposure to the pollutant named atrazine (herbicide)

52%

Khan A., Yousafzai A. M., Shah N., Muhammad, Ahmad M. S., Farooq M., Aziz F., Adnan M., Rizwan M., Jawad S. M.

Polish Journal of Environmental Studies

|

2016

|

tom 25

|

nr 5

The present study contemplates the enzymatic profile of grass carp, including lactate dehydrogenase (LDH), creatinine phosphokinases (CPK), serum glutamic-pyruvic transaminase (SGPT), and alkaline phosphatase (Alk Phosp) under atrazine’s acute toxicity effects (LC50) for 01 (15 μl/L), 02 (13 μl/L), 03 (10 μl/L), and 04 (08 μl/L) days/concentration, respectively. For analyzing the enzymatic profile we followed the biochemical analyzer set protocol (Merck micro lab 300 biochemistry analyzer) in the laboratory. Control group concentrations for LDH, CPK, SGPT, and Alk Phosp were 342 IU/ml, 7513.3 IU/ml, 46 mmol/l, and 126.6 IU/ml, respectively. After treatment LDH concentrations were 906, 851, 765, and 545 IU/ml, respectively. CPK concentrations were 1,737, 2,445, 3,718, and 5,767 IU/ml, respectively. SGPT concentrations were 27, 24.3, 13.67, and 8.67, respectively, and Alk Phosp concentrations were 50.3, 30, 22.3, and 17.6 IU/ml, respectively. Maximum inclined (P≤0.001) in concentration of LDH was observed after 24 hrs exposure because of hepatic tissue damage, resulting in increased membrane permeability causing enhanced leaching out of LDH and as LDH participates in an anaerobic pathway, so increase LDH mean increases of anaerobic metabolism resulting from depletion of energy under environmental stress conditions by atrazine, while other enzymatic components like CPK, SGPT, and Alk Phosp showed kindred attributes in their result, like all parameter concentrations showed perpetual decline (P≤0.001) in their concentrations indicating reduced enzymatic activity due to a reduction in permeability for these enzymes, forcing the enzymes to accumulate in the cells as well as decrease in enzyme synthesis due to intoxication of atrazine.

Ograniczanie wyników

Characterization and identification of gut-associated phytase-producing bacteria in some fresh water fish cultured in ponds

Effects of four host plants on biological parameters of Maconellicoccus hirsutus Green [Homoptera: Pseudococcidae] and efficacy of Anagyrus kamali Moursi [Hymenoptera: Encyrtidae]

Disrupted brain thyroid hormone homeostasis and altered thyroid hormone-dependent brain gene expression in autism spectrum disorders

On the long-term variability of temperature trends and changes in surface air temperature in Kolkata Weather Observatory, West Bengal, India

Functional characterization of human Kindlin-2 core promoter identifies a key role of SP1 in Kindlin-2 transcriptional regulation

The interaction of metal nanoparticles (copper, silver, platinum, and gold) with cell line HS-5

Salicylic acid mitigates salinity stress by improving antioxidant defence system and enhances vincristine and vinblastine alkaloids production in periwinkle [Catharanthus roseus (L.)G. Don]

Sex-dependent changes in cerebellar thyroid hormone-dependent gene expression following perinatal exposure to thimerosal in rats

Increase in the effectiveness of somatodendritic 5-HT-1A receptors in a rat model of tardive dyskinesia

Mitigation of As toxicity in wheat by exogenous application of hydroxamate siderophore of Aspergillus origin

Calcium chloride and gibberellic acid protect linseed (Linum usitatissimum L.) from NaCl stress by inducing antioxidative defence system and osmoprotectant accumulation

Retrospective study of cystic echinococcosis (CE) based on hospital record from five major metropolitan cities of Pakistan

Potential of chitosan alone and in combination with agricultural wastes against the root-knot nematode, Meloidogyne incognita infesting eggplant

Characterizing microbial populations in petroleum-contaminated soils of Swat district, Pakistan

Quantitative determination of lethal concentration Lc50 of atrazine on biochemical parameters; total protein and serum albumin of freshwater fish grass carp (Ctenopharyngodon idella)

Effect of heavy metal pollution on mineral absorption in sunflower (Helianthus annuus L.) hybrids

Different tolerance of photosynthetic apparatus to Cd stress in two rice cultivars with the same leaf Cd accumulation

Comparative study of toxicological impinge of glyphosate and atrazine (herbicide) on stress biomarkers; Blood biochemical and hematological parameters of the freshwater common carp (Cyprinus carpio)

Potential of endochitinase gene to control Fusarium wilt and early blight disease in transgenic potato lines

Enzymatic profile aactivity of grass carp (Ctenopharyngodon idella) after exposure to the pollutant named atrazine (herbicide)