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Priming is one of the most common methods improving quality of seeds. The purpose of the research was to study effects of osmopriming combined with grapefruit extract (Biosept 33 SL) treatment on zinnia seed germination, vigour and infestation with fungi at 10 and 20°C. Two seed samples, cv. Kirke and Orys, varying in initial infestation with Alternaria zinniae, were treated with Biosept 33 SL solutions at concentrations of 0.05, 0.25, 0.5 and 1.0%, during and after priming in polyethylene glycol (PEG 8000). Controls were untreated seeds, seeds treated with fungicide Sarfun T 65 DS and seeds soaked in 0.05, 0.25, 0.5 and 1.0% Biosept 33 SL solution. Priming alone, as well as priming combined with grapefruit extracts treatment, significantly improved total germination and vigour of the seeds. Impact of Biosept 33 SL treatment on seed health varied among samples and was strongly influenced by initial seed infestation with fungi, applied doses of the preparation and temperature of incubation.
Organic acids are known for their antibacterial and antifungal properties. The purpose of the research was to study the effect of acetic, ascorbic, citric and lactic acid treatments on zinnia seed germination, vigour and infestation with fungi. Two seed samples, cultivars ‘Jowita’ and ‘Scarlet Flame’, varying in fungi occurrence intensity, were treated with organic acids solutions at concentrations of 1.0, 2.5 and 5.0%. Controls were untreated seeds, seeds treated with fungicide Penncozeb 80 WP, and seeds soaked for 30 min in distilled water. Acetic acid in the highest extent limited fungal occurrence on seeds, however negatively affected seed germination and vigour. Ascorbic and citric acids had no effect on the total seed infestation with fungi. Moreover, the acids significantly stimulated growth of Botrytis cinerea on the seeds. Lactic acid decreased the number of seeds infected with fungi, especially with Alternaria zinniae and Fusarium spp., however at the highest concentration negatively affected germination parameters and deteriorated seed vigour.
The high infestation ratę of zinnia (Zinnia elegans Jacq.) seeds with fungi, especially pathogenic Alternaria zinniae, has freąuently resulted in a poor germination capacity associated with a high number of abnormal diseased seedlings. The effect of hydrogen peroxide (H,0,) treatment on the germination, vigour and health of zinnia seeds was investigated. Two samples of zinnia seeds - sample I, characterised with a Iow germination capacity (39.5%) and a high level of seed infection with ,4. zinniae (76%), and sample II, with a high germination capacity (87%) and free from this pathogen - were tested. For treatment, seeds were soaked in 3%, 6%, 9% and 12% H202 Solutions for 10, 20, 30 and 60 min. Initially, the Controls were seeds soaked in distilled water for 10, 20, 30 and 60 min, and then the results of selected treatments were compared with results obtained for untreated seeds and seeds treated with a fungicide (20% carbendazim and 45% thiram). The fungi Alternaria spp. and Fusarium spp. were freąuently identified on tested seeds. The concentration of hydrogen peroxide affected the ąuality of zinnia seeds to a higher extent than the treatment time. However, all of the treatments applied, regardless oftime and concentration of H00,, positively affected seed health, significantly reducing seed infestation by fungi in both samples. Moreover, the lowest level of A. zinniae infection was observed if seeds were soaked in 6% and 9% H,0, Solutions for 60 and 20 min, respectively. Higher concentrations of H202 (9% and 12%) negatively influenced seed germination and vigour, especially in sample II. These findings suggested that the treatment of zinnia seeds with hydrogen peroxide can effectively improve germination mostly in samples heavily infected with pathogens, which means that it should be preceded by seed health evaluation.
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