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1

Badania nad roslinami leczniczymi prowadzone w Katedrze Roslin Warzywnych i Leczniczych SGGW w Warszawie

100%
Weglarz Z., Przybyl J. L.
Herba Polonica
|
2007
|
tom 53
|
nr 2
78-79
2

Sterole w organach wegetatywnych i generatywnych wiesiołka dziwnego (Oenothera paradoxa Hudziok)

81%
Kosakowska O., Przybyl J. L., Weglarz Z.
Folia Horticulturae. Suplement
|
2006
|
nr 1
3

Porównanie zawartości związków fenolowych w wybranych populacjach kuklika pospolitego (Geum urbanum L.) pochodzących ze stanowisk naturalnych i z uprawy

81%
Kuczerenko A., Baczek K., Przybyl J. L., Weglarz Z.
Polish Journal of Agronomy
|
2014
|
nr 18
Celem pracy było określenie zróżnicowania kuklika pospolitego (Geum urbanum L.) pod względem zawartości związków fenolowych (garbników, kwasów fenolowych i eugenolu). Materiałem badawczym były organy podziemne (kłącza z korzeniami) 10 populacji kuklika rosnących na stanowiskach naturalnych i tych samych populacji wprowadzonych do uprawy. Uzyskane surowce analizowano pod względem zawartości związków fenolowych metodą HPLC i eugenolu metodą SPME-GC. W surowcu zidentyfikowano 5 związków z grupy garbników (katechinę, epikatechinę, epigalokatechinę, galusan epikatechiny, galusan epigalokatechiny) oraz kwas elagowy i kwas galusowy, a w olejku eterycznym – eugenol jako dominujący jego składnik. Badane populacje różniły się istotnie pod względem zawartości wszystkich ww. związków, których było nieznacznie więcej w surowcach pochodzących ze stanowisk naturalnych w porównaniu z surowcami pochodzącymi z uprawy.
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Accumulation of phenolics in Eleuthero (Eleutherococcus senticosus (Rupr. et Maxim.) Maxim.) as affected by plant development

81%
Baczek K., Przybyl J. L., Kosakowska O., Weglarz Z.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2017
|
tom 16
|
nr 4
The aim of the study was to determine the effect of plant age and growth phase on the accumulation of phenolics in stem bark, leaves and underground organs of Eleuthero. Their content was assessed using validated HPLC-DAD method. In underground organs and stem bark 7 phenolics were determined, i.e. eleutherosides B and E as well as caffeic, ferulic, chlorogenic, rosmarinic and protocatechuic acids. The content of eleutherosides B was significantly higher in stem bark while eleutherosides E, in underground organs. Accumulation of these compounds was the highest in the 4-year-old plants (87.43 mg·100 g–1 DW of eleutherosides B and 85.22 mg·100 g–1 DW of eleutherosides E in underground organs; 302.21 mg·100 g–1 DW of eleutherosides B and 24.89 mg·100 g–1 DW eleutherosides E in stem bark). In these organs, among identified phenolic acids chlorogenic acid was the dominant. In the leaves 4 phenolic acids (caffeic, ferulic, chlorogenic and rosmarinic acids), as well as 2 flavonoids (rutoside and hyperoside), were identified. Flavonoids and caffeic acid occurred in higher amounts at the beginning of leaf senescence, whereas the other phenolic acids – at the full vegetation. Their content was the highest in 2-year-old plants.
5
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Antimicrobial effect of an aqueous extract of Potentilla erecta rhizome

81%
Synowiec A., Gniewosz M., Baczek K., Przybyl J. L.
Herba Polonica
|
2014
|
tom 60
|
nr 2
Potentilla erecta is a therapeutic plant used in folk medicine to treat inflammatory states, wounds and diseases of the alimentary tract. The results of the study reveal the effects of an aqueous extract of P. erecta rhizome on certain microorganisms occurring in food. The main components of the extract were catechins. The extract was shown to display an inhibiting effect against Gram-positive bacteria such as Staphylococcus aureus ATCC 25923 and Bacillus subtilis ATCC 6633, as well as against yeast such as Candida lipolitica KKP 322 and Hansenula anomala R 26. The extract did inhibit the growth of Gram-negative bacteria, however, no inhibiting effects were observed on moulds in the studied range of concentrations, i.e. 0.13 to 64 mg dry matter/ml.
6

Porównanie przeciwdrobnoustrojowej aktywności etanolowego i wodnego ekstraktu z szałwii lekarskiej (Salvia officinalis L.)

81%
Gniewosz M., Krasniewska K., Weglarz Z., Przybyl J. L.
Bromatologia i Chemia Toksykologiczna
|
2012
|
tom 45
|
nr 3
7
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Content available

Accumulation of phenolic compounds in the purple betony herb (Stachys officinalis L.) originated from cultivation

81%
Baczek K., Kosakowska O., Przybyl J. L., Weglarz Z.
Herba Polonica
|
2016
|
tom 62
|
nr 2
Introduction: Purple betony (Stachys officinalis L., Lamiaceae) is a perennial of versatile medicinal usage. Nowadays, in Poland betony herb is collected exclusively from wild growing plants. Decreasing number of its natural sites results in lack of the herb supply and thus, in its limited usage. Objective: The aim of the study was to determine the effects of the age of plant and term of raw material harvest on its yield and quality in cultivation conditions. Methods: The observations were carried out on 2- and 3-year-old plants. During vegetation the herb was collected for four times. The raw material was subjected to chemical analysis. Tannins (as pyrogallol equivalent) were determined according to Polish Pharmacopoeia, phenolic acids and flavonoids – by HPLC. Results: The mass of herb, both in the second and third year, had increased from the beginning of vegetation up to seed setting. The highest content of tannins was found in the herb collected at the vegetative stage of plant development (2.05% in the second and 2.91% in the third year). Four phenolic acids (chlorogenic, ferulic, caffeic and rosmarinic acids) and five flavonoid compounds (orientin, luteolin-7-glucoside, apigenin-7-glucoside, apigenin-3-glucoside, apigenin) were identified in the obtained raw materials. In these groups, the dominant compounds were caffeic acid and apigenin. The highest content of caffeic acid was found at the beginning of plant vegetation, whereas apigenin – at the stage of full blooming and seed setting. Conclusion: In cultivation conditions, purple betony produces high mass of herb which may be used as a valuable raw material in herbal industry.
8

Content of biologically active compounds in roseroot [Rhodiola sp.] raw material of different derivation

81%
Altantsetseg K., Przybyl J. L., Weglarz Z., Geszprych A.
Herba Polonica
|
2007
|
tom 53
|
nr 4
Four populations of roseroot (Rhodiola rosea and Rh. quadrifida) originating from Poland and Mongolia were compared in respect of the content of main biologically active phenolic compounds (salidroside, p-tyrosol, trans-cinnamic alcohol, rosarin, rosavin and rosin) in the underground organs. Three solvents (75% ethanol, methanol and water) were used for the extraction of these phenolic compounds. Rh. rosea grown in Poland was characterised by similar content of salidroside and higher content of rosavin in comparison to the plants growing wild in Mongolia. The content of these compounds in Rh. quadrifida was lower than in Rh. rosea. Water appeared to be the best extraction medium for the isolation of salidroside and tyrosol. Cinnamic alcohol and its derivatives were more efficiently extracted with methanol and 75% ethanol.
9
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Seasonal variation of phenolics content in above- and underground organs of dropwort (Filipendula vulgaris Moench)

71%
Baczek K., Cygan M., Przybyl J. L., Kosakowska O., Weglarz Z.
Herba Polonica
|
2012
|
tom 58
|
nr 3
The content and chemical composition of phenolic compounds in above- and underground organs of dropwort during second year of plant vegetation were studied. Five flavonoids (hyperosid, astragalin, spireaoside, kaempferol, quercetin), 2 catechin derivatives ((+)-catechin, (-)-epigallocatechin), and 7 polyphenolic acids (ellagic, gallic, syringic, salicylic, chlorogenic, caffeic and rosmarinic) were identified in aboveground organs. Their content, both in flowers and leaves, was significantly higher at the beginning of flowering as compared with full flowering stage. In underground organs (+)-catechin and its derivatives ((-)-epigallocatechin, (-)-epigallocatechin gallate, (-)-epicatechin) as well as 2 polyphenolic acids (ellagic and gallic) were identified. Their content was not closely related to the stage of plant development.
10
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The influence of shadind on the yield and quality of southern sweet-grass (Hierochloe australis (Schrad.) Roem. and Schult.) raw material

71%
Podyma W., Baczek K., Angielczyk M., Przybyl J. L., Weglarz Z.
Herba Polonica
|
2010
|
tom 56
|
nr 4
Southern sweet-grass (Poaceae) rarely occurs in Polish coniferous or mixed forests. Leaves of this plant, rich in coumarin compounds, are mainly used as a flavouring raw material in alcohol, tobacco and cosmetic industry. The aim of the study was to investigate the influence of shading on the yield and quality of southern sweet-grass. The experiment was established at the certificated organic field of Department of Vegetable and Medicinal Plants. The sunlight was reduced up to 50 and 70% by using shading nets. The object of experiment was two-year-old plants grown on the alluvial soil. Leaves were harvested two times: in mid-May and in the last week of August (regrowth). The highest yield of leaves was observed at 50% of shading. The shaded plants were characterized by higher content of coumarin and 3,4-dihydrocoumarin and lower content of bergapten in comparison to non-shaded ones. In the leaves five phenolic acids and two flavonoids were identified.
11

Chemical diversity of silverweed (Potentilla anserina L.) growing at the edges of arable fields

71%
Angielczyk M., Baczek K., Geszprych A., Przybyl J. L., Weglarz Z.
Plant Breeding and Seed Science
|
2010
|
tom 61
12
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Chemical variability of common skullcap (Scutellaria galericulata L.) wild growing in the area of eastern Poland

71%
Kosakowska O., Baczek K., Przybyl J. L., Pioro-Jabrucka E., Weglarz Z.
Herba Polonica
|
2016
|
tom 62
|
nr 3
Introduction. In the group of plants revealing adaptogenic activity, species belonging to Scutellaria genus are considered to be the most important ones. Common skullcap (Scutellaria galericulata L.) is less known species from this genus, growing wild in Poland in wet habitats. Its herb has been used in the past as a sedative and diuretic agent. Objective. The aim of the study was to determine the variability of common skullcap in terms of the accumulation of flavonoids in the herb. Methods. The objects of the study were 17 common skullcap populations growing wild in eastern Poland. At each natural site, the list of main flowering plant species was carried out, according to Braun-Blanquet scale of plant abundance. The total content of flavonoids was determined according to EP 8th. The analysis of flavonoids was carried out using HPLC, Shimadzu chromatograph. Results. The total content of flavonoids ranged from 0.21 to 0.50% dry matter (DM). Using HPLC, eight compounds were identified within the group, with chrysin-7-O-glucuronide (30.91–589.27 mg × 100 g-1 DM), baicalin (61.90–482.93 mg × 100 g-1 DM) and scutellarin (43.77–213.54 mg × 100 g-1 DM) as dominants. Chrysin was the compound which in the highest degree differentiated investigated populations (CV=0.77). Conclusion. Obtained results indicate that investigated common skullcap populations differed in terms of the total content of flavonoids as well as the content of indentified flavonoids in the herb. However, there was no clear relationship between geographical localization of populations and the content of identified flavonoids in raw material.
13
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Accumulation of phenolic and sterol compounds in Euphorbia hirta (L.)

71%
Pioro-Jabrucka E., Pawelczak A., Przybyl J. L., Baczek K., Weglarz Z.
Herba Polonica
|
2011
|
tom 57
|
nr 2
Euphorbia hirta is an annual plant grownig commonly in the tropical and subtropical regions of Asia, Africa as well as Central and South America. Extracts from herb reveal diastolic, anti-inflammatory, diuretic and anthelminthic effects. Biologically active compounds of this plant are sterols, saponins, flavonoids and phenolic acids. The results of this study indicate that obtaining relatively high yield of herb and well germinating seeds which retain their germinability after two years of storage is possible in Poladn. Chemical analysis of the herb and callus obtained in vitro has shown that these raw materials accumulate phenolic compounds and sterols in high quantities.
14

Skład związków sterolowych w odmianach brokułu zależnie od metody uprawy

71%
Gajewski M., Przybyl J. L., Kosakowska O., Szymczak P., Bajer M.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2008
|
tom 527
Sterole roślinne wykazują różnorodną aktywność biologiczną, a w roślinie są podstawowymi składnikami strukturalnymi i funkcjonalnymi cytomembran. Celem pracy było określenie sumy i składu wolnych steroli w dwóch zróżnicowanych pod względem cech użytkowych odmianach brokułu, w zależności od metody i terminu uprawy. Badania przeprowadzono w latach 2005 i 2006. Brokuły uprawiano z rozsady na zbiór letni lub jesienny, metoda „na płask” lub na redlinach wykonanych wiosną. Wolne sterole w brokułach określano bezpośrednio po zbiorze metodą HPLC. Materiał roślinny poddano po wysuszeniu ekstrakcji w heksanie. Ekstrakty analizowano z wykorzystaniem chromatografu Shimadzu, z detektorem SPD-M10A VP. Wpływ metody uprawy okazał się istotny w zarówno przypadku sumy steroli, jak ich składu jakościowego. Ogólna zawartość wolnych steroli w częściach jadalnych brokułów wahała się w granicach 40-109 mg 100 g⁻¹ s.m. Zawartość wolnych steroli w brokułach różniła się w obu terminach uprawy. Dominującymi formami związków sterolowych były β-sitosterol i brassicasterol, ale skład i zawartość steroli zależały istotnie od odmiany brokułu.
15
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Variability of southern sweet-grass (Hierochloe australis /Schrad./ Roem. and Schult.) wild growing populations occurring in eastern Poland

61%
Baczek K., Angielczyk M., Przybyl J. L., Kosakowska O., Ejdys M., Weglarz Z.
Herba Polonica
|
2015
|
tom 61
|
nr 3
Southern sweet-grass is a perennial grass distributed through north-eastern Europe. In Poland, this rare, partially protected plant grows wild in well-lit coniferous or mixed forests. Its leaves, rich in coumarin compounds, are used for aromatization of alcohols. Taking into account high demand on this raw material and decrease in the number of southern sweetgrass populations it is recommended to introduce the plant into cultivation systems. In this study 12 populations of southern sweet-grass were selected and assessed in terms of their cultivation usefulness. The presence of associated plant species growing on its natural sites was investigated as well as light irradiance on those stands. The species was found in 3 types of forest communities. Air dry mass of leaves per plant varied in analyzed populations from 1.73 to 11.07 g. They were significantly diversified in the total content of coumarins (0.36–0.96%), flavonoids (0.09–0.26%) and polyphenolic acids (0.13–0.29%). The diversity concerning the content of coumarin, a quality indicator of leaves, was even higher. The content of this compound varied from 52.03 to 275.74 mg/100 g d. m. Among the analyzed populations, the most interesting seem to be population No. 12 (Jedwabno) and population No. 10 (Ruciane) characterized with relatively high mass of leaves and the highest content of the identified active compounds, including coumarin.
16

Intraspecific variability of fireweed (Chamaenerion angustifolium/L./Scop.) and evening primrose (Oenothera biennis L.) in respect of sterol content

61%
Weglarz Z., Kosakowska L., Pelc M., Geszprych A., Przybyl J. L., Baczek K.
Herba Polonica
|
2011
|
tom 57
|
nr 2
Composition of sterol fraction and content of identified compounds in herb of fireweed (Chamaenerion angustifolium fU Scop.) and leaves of evening primrose (Oenothera biennis L.) obtained from several populations of these species growing wild in Poland were determined by HPLC. The investigated species differed in respect of the composition and content of identified sterols. High intraspecific variability concerning accumulation of sterols was also observed. Herb of fireweed was characterised by relatively high content of p-sitosterol (85.80-171.18 mg/100 g), campesterol (24.24-334.49 mg/100 g), and p-sitosterol D-glucoside (26.33-86.32 mg/100 g). Leaves of evening primrose appeared to be much poorer source of sterols. Content of |3-sitosterol (the dominant compound in the sterol fraction) in this raw material ranged from 5.21 to 34.66 mg/100 g.
17
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Content available

Intraspecific variability of yarrow (Achillea millefolium L. s.l.) in respect of developmental and chemical traits

61%
Baczek K., Kosakowska O., Przybyl J. L., Kuzma P., Ejdys M., Obiedzinski M., Weglarz Z.
Herba Polonica
|
2015
|
tom 61
|
nr 3
The aim of study was to determine the variability of 20 yarrow populations introduced into ex situ conditions, in respect of selected developmental traits as well as content and composition of biologically active compounds (essential oil, tannins, flavonoids and phenolic acids). Field experiment was established at the Experimental Station, Department of Vegetable and Medicinal Plants. Morphological observations and harvest of raw material were carried out in the second year of plant vegetation, at the beginning of blooming. Investigated populations differed significantly in respect of developmental features as well as content and composition of identified compounds. The highest differences among the populations concerned fresh mass of herb (0.46–1.79 kg per plant), number of shoots per m2 (64–243) and length of the longest internode (42–158 mm). Total content of essential oil ranged from 0.10 to 1.00%. Among 24 identified compounds β-pinene, 1,8-cineole, terpinene-4-ol, nerolidol and chamazulene were the dominants. According to content of these compounds, three chemotypes were distinguished within investigated populations, i.e.: β-pinene, β-pinene + chamazulene and 1,8-cineole type. Content of tannins ranged from 0.38 to 0.90%. Four flavonoids were identified and apigenin 7-glucoside was present in the highest amount (from 9.87 to 475.21 mg × 100 g-1), while the highest differences between populations concerned the content of luteolin-3’,7-diglucoside. Within phenolic acids, three compounds (caffeic acid derivatives) were identified. Rosmarinic acid was the dominant one (75.64–660.54 mg × 100 g-1), while cichoric acid differentiated investigated populations the most.
18

Zrożnicowanie wewnątrzgatunkowe polskich dziko rosnących roślin leczniczych wprowadzanych do uprawy

61%
Weglarz Z., Geszprych A., Kosakowska O., Osinska E., Pelc M., Przybyl J. L.
Pamiętnik Puławski
|
2009
|
tom 151
|
nr 1
19
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Content available

Functional traits of selected clones of southern sweet-grass (Hierochloë australis /Schrad./ Roem. and Schult.)

61%
Baczek K., Angielczyk M., Przybyl J. L., Ejdys M., Geszprych A., Weglarz Z.
Herba Polonica
|
2014
|
tom 60
|
nr 3
Southern sweet-grass (Hierochloë australis /Schrad./ Roem. & Schult., Poaceae), commonly known as a bison grass, is a perennial tuft grass rarely occurring in mixed forests in Eastern and Northern Europe. The raw material collected from this plant are leaves rich in coumarin compounds (especially coumarin) responsible for sweet, specific aroma of these organs. In Poland, southern sweet-grass leaves are used mainly for alcohol products aromatisation. Growing demand for the raw material results in uncontrolled and excessive collecting of this plant. The best way to solve this problem is to introduce this plant into cultivation. Since southern sweet-grass is allogamous and heterozigotic, strong intraspecific variability of the species is observed. Fourteen clones of selected individuals from one population of southern sweet-grass naturally occurring in East Poland were compared in respect of morphological traits as well as accumulation of biologically active compounds. The clones differed significantly in the weight of leaves (3.76–22.59 g of air-dry weight per plant). The total coumarin content (determined by a spectrophotometric method) in this raw material for investigated clones ranged from 1.49 to 1.94%, flavonoids – from 0.25 to 0.55%, and phenolic acids – from 0.20 to 0.42%. Three coumarin compounds were identified by HPLC, namely coumarin, 3,4-dihydrocoumarin, and o-coumaric acid. The content of coumarin ranged from 84.00 to 310.85, 3,4-dihydrocoumarin: from 17.80 to 168.45, and o-coumaric acid: from 37.50 to 70.00 mg · 100 g-1 dry matter.
20
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Content available

Intraspecific variability of great burnet (Sanguisorba officinalis L.) in respect of sterols content

61%
Mirgos M., Przybyszewska E., Capecka E., Angielczyk M., Przybyl J. L., Baczek K., Weglarz Z.
Herba Polonica
|
2012
|
tom 58
|
nr 3
The content and composition of sterol compounds in wild growing great burnet (Sanguisorba officinalis L.) populations were determined. The herb and underground organs of this plant were collected from 10 natural sites in Poland. The HPLC analysis revealed the presence of 5 sterol compounds, namely β-sitosterol, β-sitosterol D-glucoside, campesterol, brassicasterol and stigmasterol in these raw materials. Among them, β-sitosterol Dglucoside was dominant one. The herb was richer in sterol compounds than underground organs. There were significant differences between the content of above mentioned compounds in these populations.
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